Nanopartícula pH responsiva para administração oral de antiparasitários no tratamento de peixes ornamentais

• Autor(a) principal: Patta, Ana Carolina Monge Fernandes [UNIFESP]
• Data de Publicação: 2020
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da UNIFESP
• Texto Completo: https://sucupira.capes.gov.br/sucupira/public/consultas/coleta/trabalhoConclusao/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=8465036; https://hdl.handle.net/11600/64289
• topic: Chitosan; Arginine; Alginate; Nanoparticle; Carrier; Anthelmintic Drugs; Ornamental Fish; Quitosana; Arginina; Alginato; Nanopartícula; Carreador; Fármacos Anti-Helmínticos; Peixes Ornamentais

Studies show that the global trade in ornamental fish has a high economic impact, as it involves buying and selling millions of specimens a year, generating thousands of jobs and revenues. Meanwhile, it increases the risk of cross-border spread of infectious diseases through the spread of live animals, with devastating consequences. The present study aimed to develop a strategy of innovative aspects in the treatment of endoparasites in ornamental fish, through the production, characterization and application of nanoparticles with specific molecular characteristics of biological interaction. These chitosan-N-arginine and alginate composite nanoparticles were developed as a carrier system for anthelmintic ivermectin and praziquantel drugs and with muco-adhesive and pH responsive characteristics as a function of the application medium. For the development and study, physicochemical techniques of characterization were applied and application studies of the composites containing drugs were performed by oral administration in adult ornamental fish Corydoras schwartzi, parasitized with nematodes and digenetic trematodes, for evaluation of endoparasitic treatment. Studies have shown that the particles have pH-dependent surface charge and colloidal structure characteristics. With in vivo experiments it was observed that the particles remained in the fish's gastrointestinal system for periods longer than eight hours after administration, favoring the attenuated release of the drugs and the efficacy of the treatments, which achieved a reduction of up to 95% of the fish intestinal parasites. Thus, it was evidenced that the particles were effective in transporting and releasing drugs to the target region, enabling an innovative and effective treatment. The studies also open perspectives for the use of fish as an in vivo biological model to evaluate the applicability of bioactive carrier systems.