Fractures of the proximal femur: correlation with vitamin D receptor gene polymorphism
Autor(a) principal: | |
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Data de Publicação: | 1998 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/650 http://dx.doi.org/10.1590/S0100-879X1998000700006 |
Resumo: | Fractures are the feared consequences of osteoporosis and fractures of the proximal femur (FPF) are those that involve the highest morbidity and mortality. Thus far, evaluation of bone mineral density (BMD) is the best way to determine the risk of fracture. Genetic inheritance, in turn, is one of the major determinants of BMD. A correlation between different genotypes of the vitamin D receptor (VDR) and BMD has been recently reported. On this basis, we decided to determine the importance of the determination of VDR genotype in the presence of an osteoporotic FPF in a Brazilian population. We studied three groups: group I consisted of 73 elderly subjects older than 65 years (78.5 ± 7.2 years) hospitalized for nonpathological FPF; group II consisted of 50 individuals older than 65 years (72.9 ± 5.2 years) without FPF and group III consisted of 98 young normal Brazilian individuals aged 32.6 ± 6.6 years (mean ± SD). Analysis of VDR gene polymorphism by restriction fragment length polymorphism (RFLP) was performed by PCR amplification followed by BsmI digestion of DNA isolated from peripheral leukocytes. The genotype distribution in group I was 20.5% BB, 42.5% Bb and 37% bb and did not differ significantly from the values obtained for group II (16% BB, 36% Bb and 48% bb) or for group III (10.2% BB, 47.6% Bb and 41.8% bb). No differences in genotype distribution were observed between sexes or between the young and elderly groups. We conclude that determination of VDR polymorphism is of no practical use for the prediction of FPF. Other nongenetic factors probably start to affect bone mass, the risk to fall and consequently the occurrence of osteoporotic fractures with advancing age. |
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Ramalho, Ana Claudia [UNIFESP]Lazaretti-Castro, Marise [UNIFESP]Hauache, Omar Magid [UNIFESP]Kasamatsu, Teresa Sayoko [UNIFESP]Brandão, C.Reis, André Fernandes [UNIFESP]Takata, Edmilson Takehiro [UNIFESP]Cafalli, Francisco [UNIFESP]Tavares, F.Gimeno, Suely Godoy Agostinho [UNIFESP]Vieira, J.g.h.A01Universidade Federal de São Paulo (UNIFESP)Hospital do Servidor Público Estadual de São Paulo2015-06-14T13:24:45Z2015-06-14T13:24:45Z1998-07-01Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 31, n. 7, p. 921-927, 1998.0100-879Xhttp://repositorio.unifesp.br/handle/11600/650http://dx.doi.org/10.1590/S0100-879X1998000700006S0100-879X1998000700006.pdfS0100-879X199800070000610.1590/S0100-879X1998000700006WOS:000074293300006Fractures are the feared consequences of osteoporosis and fractures of the proximal femur (FPF) are those that involve the highest morbidity and mortality. Thus far, evaluation of bone mineral density (BMD) is the best way to determine the risk of fracture. Genetic inheritance, in turn, is one of the major determinants of BMD. A correlation between different genotypes of the vitamin D receptor (VDR) and BMD has been recently reported. On this basis, we decided to determine the importance of the determination of VDR genotype in the presence of an osteoporotic FPF in a Brazilian population. We studied three groups: group I consisted of 73 elderly subjects older than 65 years (78.5 ± 7.2 years) hospitalized for nonpathological FPF; group II consisted of 50 individuals older than 65 years (72.9 ± 5.2 years) without FPF and group III consisted of 98 young normal Brazilian individuals aged 32.6 ± 6.6 years (mean ± SD). Analysis of VDR gene polymorphism by restriction fragment length polymorphism (RFLP) was performed by PCR amplification followed by BsmI digestion of DNA isolated from peripheral leukocytes. The genotype distribution in group I was 20.5% BB, 42.5% Bb and 37% bb and did not differ significantly from the values obtained for group II (16% BB, 36% Bb and 48% bb) or for group III (10.2% BB, 47.6% Bb and 41.8% bb). No differences in genotype distribution were observed between sexes or between the young and elderly groups. We conclude that determination of VDR polymorphism is of no practical use for the prediction of FPF. Other nongenetic factors probably start to affect bone mass, the risk to fall and consequently the occurrence of osteoporotic fractures with advancing age.A01Universidade Federal de São Paulo (UNIFESP)Hospital do Servidor Público Estadual de São PauloUNIFESP, EPM, São PauloSciELO921-927engAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Researchosteoporosisfracture of proximal femur (FPF)vitamin D receptor polymorphismFractures of the proximal femur: correlation with vitamin D receptor gene polymorphisminfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALS0100-879X1998000700006.pdfapplication/pdf37954${dspace.ui.url}/bitstream/11600/650/1/S0100-879X1998000700006.pdf7d380f93a76d4be119693488e2535b61MD51open accessTEXTS0100-879X1998000700006.pdf.txtS0100-879X1998000700006.pdf.txtExtracted texttext/plain25059${dspace.ui.url}/bitstream/11600/650/2/S0100-879X1998000700006.pdf.txt99285aefbb8385e6ae7a7d980776126aMD52open access11600/6502022-09-27 09:48:32.946open accessoai:repositorio.unifesp.br:11600/650Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652022-09-27T12:48:32Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Fractures of the proximal femur: correlation with vitamin D receptor gene polymorphism |
title |
Fractures of the proximal femur: correlation with vitamin D receptor gene polymorphism |
spellingShingle |
Fractures of the proximal femur: correlation with vitamin D receptor gene polymorphism Ramalho, Ana Claudia [UNIFESP] osteoporosis fracture of proximal femur (FPF) vitamin D receptor polymorphism |
title_short |
Fractures of the proximal femur: correlation with vitamin D receptor gene polymorphism |
title_full |
Fractures of the proximal femur: correlation with vitamin D receptor gene polymorphism |
title_fullStr |
Fractures of the proximal femur: correlation with vitamin D receptor gene polymorphism |
title_full_unstemmed |
Fractures of the proximal femur: correlation with vitamin D receptor gene polymorphism |
title_sort |
Fractures of the proximal femur: correlation with vitamin D receptor gene polymorphism |
author |
Ramalho, Ana Claudia [UNIFESP] |
author_facet |
Ramalho, Ana Claudia [UNIFESP] Lazaretti-Castro, Marise [UNIFESP] Hauache, Omar Magid [UNIFESP] Kasamatsu, Teresa Sayoko [UNIFESP] Brandão, C. Reis, André Fernandes [UNIFESP] Takata, Edmilson Takehiro [UNIFESP] Cafalli, Francisco [UNIFESP] Tavares, F. Gimeno, Suely Godoy Agostinho [UNIFESP] Vieira, J.g.h. |
author_role |
author |
author2 |
Lazaretti-Castro, Marise [UNIFESP] Hauache, Omar Magid [UNIFESP] Kasamatsu, Teresa Sayoko [UNIFESP] Brandão, C. Reis, André Fernandes [UNIFESP] Takata, Edmilson Takehiro [UNIFESP] Cafalli, Francisco [UNIFESP] Tavares, F. Gimeno, Suely Godoy Agostinho [UNIFESP] Vieira, J.g.h. |
author2_role |
author author author author author author author author author author |
dc.contributor.institution.none.fl_str_mv |
A01 Universidade Federal de São Paulo (UNIFESP) Hospital do Servidor Público Estadual de São Paulo |
dc.contributor.author.fl_str_mv |
Ramalho, Ana Claudia [UNIFESP] Lazaretti-Castro, Marise [UNIFESP] Hauache, Omar Magid [UNIFESP] Kasamatsu, Teresa Sayoko [UNIFESP] Brandão, C. Reis, André Fernandes [UNIFESP] Takata, Edmilson Takehiro [UNIFESP] Cafalli, Francisco [UNIFESP] Tavares, F. Gimeno, Suely Godoy Agostinho [UNIFESP] Vieira, J.g.h. |
dc.subject.eng.fl_str_mv |
osteoporosis fracture of proximal femur (FPF) vitamin D receptor polymorphism |
topic |
osteoporosis fracture of proximal femur (FPF) vitamin D receptor polymorphism |
description |
Fractures are the feared consequences of osteoporosis and fractures of the proximal femur (FPF) are those that involve the highest morbidity and mortality. Thus far, evaluation of bone mineral density (BMD) is the best way to determine the risk of fracture. Genetic inheritance, in turn, is one of the major determinants of BMD. A correlation between different genotypes of the vitamin D receptor (VDR) and BMD has been recently reported. On this basis, we decided to determine the importance of the determination of VDR genotype in the presence of an osteoporotic FPF in a Brazilian population. We studied three groups: group I consisted of 73 elderly subjects older than 65 years (78.5 ± 7.2 years) hospitalized for nonpathological FPF; group II consisted of 50 individuals older than 65 years (72.9 ± 5.2 years) without FPF and group III consisted of 98 young normal Brazilian individuals aged 32.6 ± 6.6 years (mean ± SD). Analysis of VDR gene polymorphism by restriction fragment length polymorphism (RFLP) was performed by PCR amplification followed by BsmI digestion of DNA isolated from peripheral leukocytes. The genotype distribution in group I was 20.5% BB, 42.5% Bb and 37% bb and did not differ significantly from the values obtained for group II (16% BB, 36% Bb and 48% bb) or for group III (10.2% BB, 47.6% Bb and 41.8% bb). No differences in genotype distribution were observed between sexes or between the young and elderly groups. We conclude that determination of VDR polymorphism is of no practical use for the prediction of FPF. Other nongenetic factors probably start to affect bone mass, the risk to fall and consequently the occurrence of osteoporotic fractures with advancing age. |
publishDate |
1998 |
dc.date.issued.fl_str_mv |
1998-07-01 |
dc.date.accessioned.fl_str_mv |
2015-06-14T13:24:45Z |
dc.date.available.fl_str_mv |
2015-06-14T13:24:45Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 31, n. 7, p. 921-927, 1998. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/650 http://dx.doi.org/10.1590/S0100-879X1998000700006 |
dc.identifier.issn.none.fl_str_mv |
0100-879X |
dc.identifier.file.none.fl_str_mv |
S0100-879X1998000700006.pdf |
dc.identifier.scielo.none.fl_str_mv |
S0100-879X1998000700006 |
dc.identifier.doi.none.fl_str_mv |
10.1590/S0100-879X1998000700006 |
dc.identifier.wos.none.fl_str_mv |
WOS:000074293300006 |
identifier_str_mv |
Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 31, n. 7, p. 921-927, 1998. 0100-879X S0100-879X1998000700006.pdf S0100-879X1998000700006 10.1590/S0100-879X1998000700006 WOS:000074293300006 |
url |
http://repositorio.unifesp.br/handle/11600/650 http://dx.doi.org/10.1590/S0100-879X1998000700006 |
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eng |
language |
eng |
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Brazilian Journal of Medical and Biological Research |
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info:eu-repo/semantics/openAccess |
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openAccess |
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921-927 |
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Associação Brasileira de Divulgação Científica |
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Associação Brasileira de Divulgação Científica |
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Repositório Institucional da UNIFESP |
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Repositório Institucional da UNIFESP |
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