Rac1/WAVE2 and Cdc42/N-WASP Participation in Actin-Dependent Host Cell Invasion by Extracellular Amastigotes of Trypanosoma cruzi
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | https://repositorio.unifesp.br/handle/11600/54106 http://dx.doi.org/10.3389/fmicb.2018.00360 |
Resumo: | This study evaluated the participation of host cell Rho-family GTPases and their effector proteins in the actin-dependent invasion by Trypanosoma cruzi extracellular amastigotes (EAs). We observed that all proteins were recruited and colocalized with actin at EA invasion sites in live or fixed cells. EA internalization was inhibited in cells depleted in Rac1, N-WASP, and WAVE2. Time-lapse experiments with Rac1, N-WASP and WAVE2 depleted cells revealed that EA internalization kinetics is delayed even though no differences were observed in the proportion of EA-induced actin recruitment in these groups. Overexpression of constitutively active constructs of Rac1 and RhoA altered the morphology of actin recruitments to EA invasion sites. Additionally, EA internalization was increased in cells overexpressing CA-Rac1 but inhibited in cells overexpressing CA-RhoA. WT-Cdc42 expression increased EA internalization, but curiously, CA-Cdc42 inhibited it. Altogether, these results corroborate the hypothesis of EA internalization in non-phagocytic cells by a phagocytosis-like mechanism and present Rac1 as the key Rho-family GTPase in this process. |
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Bonfim-Melo, Alexis [UNIFESP]Ferreira, Eden R. [UNIFESP]Mortara, Renato A. [UNIFESP]2020-07-08T13:09:38Z2020-07-08T13:09:38Z2018Frontiers In Microbiology. Lausanne, v. 9, p. -, 2018.1664-302Xhttps://repositorio.unifesp.br/handle/11600/54106http://dx.doi.org/10.3389/fmicb.2018.00360WOS000426303300002.pdf10.3389/fmicb.2018.00360WOS:000426303300002This study evaluated the participation of host cell Rho-family GTPases and their effector proteins in the actin-dependent invasion by Trypanosoma cruzi extracellular amastigotes (EAs). We observed that all proteins were recruited and colocalized with actin at EA invasion sites in live or fixed cells. EA internalization was inhibited in cells depleted in Rac1, N-WASP, and WAVE2. Time-lapse experiments with Rac1, N-WASP and WAVE2 depleted cells revealed that EA internalization kinetics is delayed even though no differences were observed in the proportion of EA-induced actin recruitment in these groups. Overexpression of constitutively active constructs of Rac1 and RhoA altered the morphology of actin recruitments to EA invasion sites. Additionally, EA internalization was increased in cells overexpressing CA-Rac1 but inhibited in cells overexpressing CA-RhoA. WT-Cdc42 expression increased EA internalization, but curiously, CA-Cdc42 inhibited it. Altogether, these results corroborate the hypothesis of EA internalization in non-phagocytic cells by a phagocytosis-like mechanism and present Rac1 as the key Rho-family GTPase in this process.Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)Conselho Nacional de Desenvolvimento Cientifico e TecnologicoUniv Fed Sao Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, Sao Paulo, BrazilUniv Fed Sao Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, Sao Paulo, BrazilFAPESP: 2012/21335-8, 2011/51475-3CNPq: 302068/2016-3Web of Science-engFrontiers Media SaFrontiers In MicrobiologyTrypanosoma cruziextracellular amastigotesRho GTPasesactinhost cell invasionRac1/WAVE2 and Cdc42/N-WASP Participation in Actin-Dependent Host Cell Invasion by Extracellular Amastigotes of Trypanosoma cruziinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleLausanne9info:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000426303300002.pdfapplication/pdf4077894${dspace.ui.url}/bitstream/11600/54106/1/WOS000426303300002.pdf131345364f18394db85e80c7df8336d9MD51open accessTEXTWOS000426303300002.pdf.txtWOS000426303300002.pdf.txtExtracted texttext/plain61642${dspace.ui.url}/bitstream/11600/54106/2/WOS000426303300002.pdf.txt9d7c54c278f788d5bb92b72491b5e9d0MD52open accessTHUMBNAILWOS000426303300002.pdf.jpgWOS000426303300002.pdf.jpgIM Thumbnailimage/jpeg7338${dspace.ui.url}/bitstream/11600/54106/4/WOS000426303300002.pdf.jpg4cbd293c41a37b3f11219107c9391e16MD54open access11600/541062022-08-01 08:05:25.729open accessoai:repositorio.unifesp.br:11600/54106Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652022-08-01T11:05:25Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Rac1/WAVE2 and Cdc42/N-WASP Participation in Actin-Dependent Host Cell Invasion by Extracellular Amastigotes of Trypanosoma cruzi |
title |
Rac1/WAVE2 and Cdc42/N-WASP Participation in Actin-Dependent Host Cell Invasion by Extracellular Amastigotes of Trypanosoma cruzi |
spellingShingle |
Rac1/WAVE2 and Cdc42/N-WASP Participation in Actin-Dependent Host Cell Invasion by Extracellular Amastigotes of Trypanosoma cruzi Bonfim-Melo, Alexis [UNIFESP] Trypanosoma cruzi extracellular amastigotes Rho GTPases actin host cell invasion |
title_short |
Rac1/WAVE2 and Cdc42/N-WASP Participation in Actin-Dependent Host Cell Invasion by Extracellular Amastigotes of Trypanosoma cruzi |
title_full |
Rac1/WAVE2 and Cdc42/N-WASP Participation in Actin-Dependent Host Cell Invasion by Extracellular Amastigotes of Trypanosoma cruzi |
title_fullStr |
Rac1/WAVE2 and Cdc42/N-WASP Participation in Actin-Dependent Host Cell Invasion by Extracellular Amastigotes of Trypanosoma cruzi |
title_full_unstemmed |
Rac1/WAVE2 and Cdc42/N-WASP Participation in Actin-Dependent Host Cell Invasion by Extracellular Amastigotes of Trypanosoma cruzi |
title_sort |
Rac1/WAVE2 and Cdc42/N-WASP Participation in Actin-Dependent Host Cell Invasion by Extracellular Amastigotes of Trypanosoma cruzi |
author |
Bonfim-Melo, Alexis [UNIFESP] |
author_facet |
Bonfim-Melo, Alexis [UNIFESP] Ferreira, Eden R. [UNIFESP] Mortara, Renato A. [UNIFESP] |
author_role |
author |
author2 |
Ferreira, Eden R. [UNIFESP] Mortara, Renato A. [UNIFESP] |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Bonfim-Melo, Alexis [UNIFESP] Ferreira, Eden R. [UNIFESP] Mortara, Renato A. [UNIFESP] |
dc.subject.eng.fl_str_mv |
Trypanosoma cruzi extracellular amastigotes Rho GTPases actin host cell invasion |
topic |
Trypanosoma cruzi extracellular amastigotes Rho GTPases actin host cell invasion |
description |
This study evaluated the participation of host cell Rho-family GTPases and their effector proteins in the actin-dependent invasion by Trypanosoma cruzi extracellular amastigotes (EAs). We observed that all proteins were recruited and colocalized with actin at EA invasion sites in live or fixed cells. EA internalization was inhibited in cells depleted in Rac1, N-WASP, and WAVE2. Time-lapse experiments with Rac1, N-WASP and WAVE2 depleted cells revealed that EA internalization kinetics is delayed even though no differences were observed in the proportion of EA-induced actin recruitment in these groups. Overexpression of constitutively active constructs of Rac1 and RhoA altered the morphology of actin recruitments to EA invasion sites. Additionally, EA internalization was increased in cells overexpressing CA-Rac1 but inhibited in cells overexpressing CA-RhoA. WT-Cdc42 expression increased EA internalization, but curiously, CA-Cdc42 inhibited it. Altogether, these results corroborate the hypothesis of EA internalization in non-phagocytic cells by a phagocytosis-like mechanism and present Rac1 as the key Rho-family GTPase in this process. |
publishDate |
2018 |
dc.date.issued.fl_str_mv |
2018 |
dc.date.accessioned.fl_str_mv |
2020-07-08T13:09:38Z |
dc.date.available.fl_str_mv |
2020-07-08T13:09:38Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Frontiers In Microbiology. Lausanne, v. 9, p. -, 2018. |
dc.identifier.uri.fl_str_mv |
https://repositorio.unifesp.br/handle/11600/54106 http://dx.doi.org/10.3389/fmicb.2018.00360 |
dc.identifier.issn.none.fl_str_mv |
1664-302X |
dc.identifier.file.none.fl_str_mv |
WOS000426303300002.pdf |
dc.identifier.doi.none.fl_str_mv |
10.3389/fmicb.2018.00360 |
dc.identifier.wos.none.fl_str_mv |
WOS:000426303300002 |
identifier_str_mv |
Frontiers In Microbiology. Lausanne, v. 9, p. -, 2018. 1664-302X WOS000426303300002.pdf 10.3389/fmicb.2018.00360 WOS:000426303300002 |
url |
https://repositorio.unifesp.br/handle/11600/54106 http://dx.doi.org/10.3389/fmicb.2018.00360 |
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Frontiers In Microbiology |
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Frontiers Media Sa |
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Frontiers Media Sa |
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