Immunohistochemical evaluation of fibrillar components of the extracellular matrix of transversalis fascia and anterior abdominal rectus sheath in men with inguinal hernia

Detalhes bibliográficos
Autor(a) principal: Gonçalves, Rogério de Oliveira [UNIFESP]
Data de Publicação: 2014
Outros Autores: Silva, Evandro de Moraes e, Lopes Filho, Gaspar de Jesus [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1590/S0100-69912014000100006
http://repositorio.unifesp.br/handle/11600/8151
Resumo: OBJECTIVE: to evaluate the role of fibrillar extracellular matrix components in the pathogenesis of inguinal hernias. METHODS: samples of the transverse fascia and of the anterior sheath of the rectus abdominis muscle were collected from 40 men aged between 20 and 60 years with type II and IIIA Nyhus inguinal hernia and from 10 fresh male cadavers (controls) without hernia in the same age range. The staining technique was immunohistochemistry for collagen I, collagen III and elastic fibers; quantification of fibrillar components was performed with an image analysis processing software. RESULTS: no statistically significant differences were found in the amount of elastic fibers, collagen I and collagen III, and the ratio of collagen I / III among patients with inguinal hernia when compared with subjects without hernia. CONCLUSION: the amount of fibrillar extracellular matrix components did not change in patients with and without inguinal hernia.
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spelling Immunohistochemical evaluation of fibrillar components of the extracellular matrix of transversalis fascia and anterior abdominal rectus sheath in men with inguinal herniaAvaliação imunoistoquímica dos componentes fibrilares na matriz extracelular da fáscia transversal e da bainha do músculo reto abdominal de homens portadores de hérnia inguinalImmunohistochemistryCollagenExtracellular matrixFasciaHernia, inguinalImunoistoquímicaColágenoMatriz extracelularFásciaHérnia inguinalOBJECTIVE: to evaluate the role of fibrillar extracellular matrix components in the pathogenesis of inguinal hernias. METHODS: samples of the transverse fascia and of the anterior sheath of the rectus abdominis muscle were collected from 40 men aged between 20 and 60 years with type II and IIIA Nyhus inguinal hernia and from 10 fresh male cadavers (controls) without hernia in the same age range. The staining technique was immunohistochemistry for collagen I, collagen III and elastic fibers; quantification of fibrillar components was performed with an image analysis processing software. RESULTS: no statistically significant differences were found in the amount of elastic fibers, collagen I and collagen III, and the ratio of collagen I / III among patients with inguinal hernia when compared with subjects without hernia. CONCLUSION: the amount of fibrillar extracellular matrix components did not change in patients with and without inguinal hernia.OBJETIVO: avaliar a participação dos componentes fibrilares da matriz extracelular na etiopatogenia das hérnias inguinais. MÉTODOS:foram retiradas amostras da fáscia transversal e da bainha anterior do músculo reto abdominal de 40 homens na faixa etária entre 20 e 60 anos, portadores de hérnia inguinal tipo II e IIIA de Nyhus e de 10 controles constituído por cadáveres frescos, na mesma faixa etária, sem hérnia. A técnica de coloração foi a imunoistoquímica para colágeno I, colágeno III e fibras elásticas e a quantificação dos componentes fibrilares foi realizada através de sistema computadorizado e software de processamento e análise de imagem. RESULTADOS: não foram encontradas diferenças estatisticamente significantes na quantidade de fibras elásticas, colágeno I e colágeno III, e na proporção de colágeno I/III entre os pacientes portadores de hérnia inguinal em comparação com indivíduos sem hérnia. CONCLUSÃO:a quantidade dos componentes fibrilares da matriz extracelular não se alterou nos pacientes com e sem hérnia inguinal.UnifoaUNIFESP-EPMUNIFESP, EPMSciELOColégio Brasileiro de CirurgiõesUnifoaUniversidade Federal de São Paulo (UNIFESP)Gonçalves, Rogério de Oliveira [UNIFESP]Silva, Evandro de Moraes eLopes Filho, Gaspar de Jesus [UNIFESP]2015-06-14T13:46:50Z2015-06-14T13:46:50Z2014-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion23-29application/pdfhttp://dx.doi.org/10.1590/S0100-69912014000100006Revista do Colégio Brasileiro de Cirurgiões. Colégio Brasileiro de Cirurgiões, v. 41, n. 1, p. 23-29, 2014.10.1590/S0100-69912014000100006S0100-69912014000100023.pdf0100-6991S0100-69912014000100023http://repositorio.unifesp.br/handle/11600/8151engRevista do Colégio Brasileiro de Cirurgiõesinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-07-29T16:10:03Zoai:repositorio.unifesp.br/:11600/8151Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-07-29T16:10:03Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Immunohistochemical evaluation of fibrillar components of the extracellular matrix of transversalis fascia and anterior abdominal rectus sheath in men with inguinal hernia
Avaliação imunoistoquímica dos componentes fibrilares na matriz extracelular da fáscia transversal e da bainha do músculo reto abdominal de homens portadores de hérnia inguinal
title Immunohistochemical evaluation of fibrillar components of the extracellular matrix of transversalis fascia and anterior abdominal rectus sheath in men with inguinal hernia
spellingShingle Immunohistochemical evaluation of fibrillar components of the extracellular matrix of transversalis fascia and anterior abdominal rectus sheath in men with inguinal hernia
Gonçalves, Rogério de Oliveira [UNIFESP]
Immunohistochemistry
Collagen
Extracellular matrix
Fascia
Hernia, inguinal
Imunoistoquímica
Colágeno
Matriz extracelular
Fáscia
Hérnia inguinal
title_short Immunohistochemical evaluation of fibrillar components of the extracellular matrix of transversalis fascia and anterior abdominal rectus sheath in men with inguinal hernia
title_full Immunohistochemical evaluation of fibrillar components of the extracellular matrix of transversalis fascia and anterior abdominal rectus sheath in men with inguinal hernia
title_fullStr Immunohistochemical evaluation of fibrillar components of the extracellular matrix of transversalis fascia and anterior abdominal rectus sheath in men with inguinal hernia
title_full_unstemmed Immunohistochemical evaluation of fibrillar components of the extracellular matrix of transversalis fascia and anterior abdominal rectus sheath in men with inguinal hernia
title_sort Immunohistochemical evaluation of fibrillar components of the extracellular matrix of transversalis fascia and anterior abdominal rectus sheath in men with inguinal hernia
author Gonçalves, Rogério de Oliveira [UNIFESP]
author_facet Gonçalves, Rogério de Oliveira [UNIFESP]
Silva, Evandro de Moraes e
Lopes Filho, Gaspar de Jesus [UNIFESP]
author_role author
author2 Silva, Evandro de Moraes e
Lopes Filho, Gaspar de Jesus [UNIFESP]
author2_role author
author
dc.contributor.none.fl_str_mv Unifoa
Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Gonçalves, Rogério de Oliveira [UNIFESP]
Silva, Evandro de Moraes e
Lopes Filho, Gaspar de Jesus [UNIFESP]
dc.subject.por.fl_str_mv Immunohistochemistry
Collagen
Extracellular matrix
Fascia
Hernia, inguinal
Imunoistoquímica
Colágeno
Matriz extracelular
Fáscia
Hérnia inguinal
topic Immunohistochemistry
Collagen
Extracellular matrix
Fascia
Hernia, inguinal
Imunoistoquímica
Colágeno
Matriz extracelular
Fáscia
Hérnia inguinal
description OBJECTIVE: to evaluate the role of fibrillar extracellular matrix components in the pathogenesis of inguinal hernias. METHODS: samples of the transverse fascia and of the anterior sheath of the rectus abdominis muscle were collected from 40 men aged between 20 and 60 years with type II and IIIA Nyhus inguinal hernia and from 10 fresh male cadavers (controls) without hernia in the same age range. The staining technique was immunohistochemistry for collagen I, collagen III and elastic fibers; quantification of fibrillar components was performed with an image analysis processing software. RESULTS: no statistically significant differences were found in the amount of elastic fibers, collagen I and collagen III, and the ratio of collagen I / III among patients with inguinal hernia when compared with subjects without hernia. CONCLUSION: the amount of fibrillar extracellular matrix components did not change in patients with and without inguinal hernia.
publishDate 2014
dc.date.none.fl_str_mv 2014-01-01
2015-06-14T13:46:50Z
2015-06-14T13:46:50Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0100-69912014000100006
Revista do Colégio Brasileiro de Cirurgiões. Colégio Brasileiro de Cirurgiões, v. 41, n. 1, p. 23-29, 2014.
10.1590/S0100-69912014000100006
S0100-69912014000100023.pdf
0100-6991
S0100-69912014000100023
http://repositorio.unifesp.br/handle/11600/8151
url http://dx.doi.org/10.1590/S0100-69912014000100006
http://repositorio.unifesp.br/handle/11600/8151
identifier_str_mv Revista do Colégio Brasileiro de Cirurgiões. Colégio Brasileiro de Cirurgiões, v. 41, n. 1, p. 23-29, 2014.
10.1590/S0100-69912014000100006
S0100-69912014000100023.pdf
0100-6991
S0100-69912014000100023
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Revista do Colégio Brasileiro de Cirurgiões
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 23-29
application/pdf
dc.publisher.none.fl_str_mv Colégio Brasileiro de Cirurgiões
publisher.none.fl_str_mv Colégio Brasileiro de Cirurgiões
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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