Avaliação do potencial de aplicação biológica de nanopartículas ferrimagnéticas conjugadas ou não a Quantum Dots

• Autor(a) principal: Kawasaki, Karine Akemi [UNIFESP]
• Data de Publicação: 2019
• Outros Autores: http://lattes.cnpq.br/5841052829044974
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da UNIFESP
• Texto Completo: https://sucupira.capes.gov.br/sucupira/public/consultas/coleta/trabalhoConclusao/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=7993093; https://repositorio.unifesp.br/handle/11600/59228
• topic: Ferrimagnetic Nanoparticles; Quantum Dots; Cancer Therapy; Dendritic Cells; B16F10-Nex2; Nanopartículas Ferrimagnéticas; Quantum Dots; Terapia Antitumoral; Células Dendríticas; B16F10-Nex2

New therapeutic strategies for cancer treatment have often been developed to enhance responses and lessen side effects. One of these strategies is the use of nanoparticles, which can be used as carriers, adjuvants, nanovacins or as biomarkers. Ferrimagnetic nanoparticles of magnetite and maghemite have been well tolerated in therapies and diagnostics because they are considered biocompatible. Moreover, the effectiveness of these particles can be improved by concentrating them at the action site using a magnetic field. However, it is difficult to map the pathway of these nanoparticles because of their poor visibility in biological systems. To solve this problem, the use of nanoparticles associated with Quantum Dots has become a major facilitator of this endeavor. Quantum Dots have also been employed in cancer therapy studies, delaying tumor development. Thus, we developed a new ferrimagnetic nanoparticle aggregated with cadmium telluride Quantum Dots, called in here composite. In B16F10-Nex2 murine melanoma tumor cells, the composite was toxic by inhibiting cell proliferation in vitro in a dose-dependent manner, and the nanoparticle internalization was ATP-dependent. In bone marrow-derived dendritic cells (BMDCs), the composite was not toxic at any of the concentrations analyzed, with an ATP-dependent mechanism of internalization. Particle internalization in BMDCs, assessed by fluorescence of cadmium telluride Quantum Dots, occurred in a dose-dependent manner. The composite induced a moderate inflammatory profile in these cells in vitro, with activation of the NLRP3 inflammasome (increased IL-1β levels expressed), and increased IL-6 and TNF expression. We concluded that the composite was toxic to B16F10-Nex2 murine melanoma cells, and well tolerated by BMDCs, inducing a moderate inflammatory profile in them. Thus, in this study we performed the initial characterization of these ferrimagnetic nanoparticles agregated to Quantum dots, which demonstrated a promising use in cancer therapy, as well as a biomarker.