Effects of topical 2% cyclosporine A on the corneas of dogs subjected to lamellar keratoplasty with a graft of equine pericardium preserved in glycerin: clinical and morphological evaluation

Detalhes bibliográficos
Autor(a) principal: Teixeira, Roseli Borges
Data de Publicação: 2004
Outros Autores: Barros, Paulo Sergio De Moraes, Martins, Maria Cristina [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1590/S0103-84782004000100020
http://repositorio.unifesp.br/handle/11600/2015
Resumo: The effects of topical 2% cyclosporine A on the cornea of dogs subjected to experimental lamellar keratoplasty with an equine pericardial graft were evaluated. Ten dogs were grouped to be evaluated 3, 7, 15, 30 and 60 days after surgery. Animals received bilateral grafts followed by the application of a 2% cyclosporine A ointment on the left eye (treated eye) and the ointment base on the right eye (control eye) twice a day. The ophthalmic evaluation showed profound bilateral blepharospasm, photophobia and a mucous secretion until the 7th day after surgery; corneal vascularization starting in the limbus was observed as early as the 3rd day in both eyes; opacification and vascularization were more intense in the treated cornea on days 15 and 30 after surgery. Vascularization was still evident on the 60th day, and looked similar in treated eyes and control eyes. The histologic evaluation showed a complete bilateral reepithelization and corneal vascularization three days after the surgery; intense vascularization in both eyes on days 15 and 30, that was much more pronounced in the treated cornea; and a bilateral predominance of polymorphonuclear cells until day 15, and mononuclear cells on day 30. Intact epithelium and stroma with new vessels, as well as graft absorption, in both eyes, were seen on the 60th day. The lamellar keratoplasty with equine pericardial graft was an effective model to study the inflammatory kinetics and corneal vascularization phenomenon. In this study, cyclosporine A did not inhibit corneal vascularization and it did not interfere in the corneal cicatricial process.
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spelling Effects of topical 2% cyclosporine A on the corneas of dogs subjected to lamellar keratoplasty with a graft of equine pericardium preserved in glycerin: clinical and morphological evaluationEfeitos da aplicação tópica da ciclosporina A a 2% sobre a córnea de cães submetidos à ceratoplastia lamelar com implante de pericárdio de eqüino preservado em glicerina: avaliação clínica e morfológicacyclosporinecorneapericardiumvascularizationkeratoplastydogsciclosporinacórneapericárdiovascularizaçãoceratoplastiacãesThe effects of topical 2% cyclosporine A on the cornea of dogs subjected to experimental lamellar keratoplasty with an equine pericardial graft were evaluated. Ten dogs were grouped to be evaluated 3, 7, 15, 30 and 60 days after surgery. Animals received bilateral grafts followed by the application of a 2% cyclosporine A ointment on the left eye (treated eye) and the ointment base on the right eye (control eye) twice a day. The ophthalmic evaluation showed profound bilateral blepharospasm, photophobia and a mucous secretion until the 7th day after surgery; corneal vascularization starting in the limbus was observed as early as the 3rd day in both eyes; opacification and vascularization were more intense in the treated cornea on days 15 and 30 after surgery. Vascularization was still evident on the 60th day, and looked similar in treated eyes and control eyes. The histologic evaluation showed a complete bilateral reepithelization and corneal vascularization three days after the surgery; intense vascularization in both eyes on days 15 and 30, that was much more pronounced in the treated cornea; and a bilateral predominance of polymorphonuclear cells until day 15, and mononuclear cells on day 30. Intact epithelium and stroma with new vessels, as well as graft absorption, in both eyes, were seen on the 60th day. The lamellar keratoplasty with equine pericardial graft was an effective model to study the inflammatory kinetics and corneal vascularization phenomenon. In this study, cyclosporine A did not inhibit corneal vascularization and it did not interfere in the corneal cicatricial process.Foram avaliados os efeitos da ciclosporina A a 2% sobre a córnea de cães submetidos à ceratoplastia lamelar experimental com implante de pericárdio de equino. Dez cães foram divididos em grupos para estudo aos três, sete, 15, 30 e 60 dias de pós-operatório, recebendo implantes bilaterais e em seguida aplicação da pomada com ciclosporina A a 2% no olho esquerdo (olho tratado)e somente a base da mesma pomada no olho direito (olho controle), duas vezes ao dia. À avaliação oftalmológica observou-se, bilateralmente, blefarospasmo, fotofobia e secreção mucosa evidentes até 7 dias de pós-operatório; início de vascularização a partir do limbo aos três dias, bilateralmente; opacidade e vascularização mais intensos em córnea esquerda aos 15 e 30 dias; vascularização ainda evidente, bilateralmente, com aspecto semelhante aos 60 dias. À avaliação histopatológica observou-se, bilateralmente, completa reepitelização e vascularização aos três dias de pós-operatório; vascularização intensa aos 15 e 30 dias, bilateralmente, porém acentuada na córnea esquerda; predomínio de polimorfonucleares até os 15 dias e mononucleares aos 30 dias, bilateralmente; epitélio e estroma íntegros com vasos ainda evidentes e implante incorporado ao estroma, bilateralmente, aos 60 dias. A utilização do implante de pericárdio de equino em ceratoplastias lamelares em cães constitui uma técnica eficaz, aplicável ao estudo da cinética inflamatória e fenômeno de vascularização. A ciclosporina A a 2% não inibiu ou diminuiu a formação vascular na córnea e também não interferiu no processo cicatricial neste estudo.Universidade de São Paulo Faculdade de Medicina Veterinária e Zootecnia Department of SurgeryUNIFESP-EPM Department of OphthalmologyUNIFESP, EPM, Department of OphthalmologySciELOFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade Federal de Santa MariaUniversidade de São Paulo (USP)Universidade Federal de São Paulo (UNIFESP)Teixeira, Roseli BorgesBarros, Paulo Sergio De MoraesMartins, Maria Cristina [UNIFESP]2015-06-14T13:30:19Z2015-06-14T13:30:19Z2004-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion131-138application/pdfhttp://dx.doi.org/10.1590/S0103-84782004000100020Ciência Rural. Universidade Federal de Santa Maria, v. 34, n. 1, p. 131-138, 2004.10.1590/S0103-84782004000100020S0103-84782004000100020.pdf0103-8478S0103-84782004000100020http://repositorio.unifesp.br/handle/11600/2015engCiência Ruralinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-04T11:52:23Zoai:repositorio.unifesp.br/:11600/2015Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-04T11:52:23Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Effects of topical 2% cyclosporine A on the corneas of dogs subjected to lamellar keratoplasty with a graft of equine pericardium preserved in glycerin: clinical and morphological evaluation
Efeitos da aplicação tópica da ciclosporina A a 2% sobre a córnea de cães submetidos à ceratoplastia lamelar com implante de pericárdio de eqüino preservado em glicerina: avaliação clínica e morfológica
title Effects of topical 2% cyclosporine A on the corneas of dogs subjected to lamellar keratoplasty with a graft of equine pericardium preserved in glycerin: clinical and morphological evaluation
spellingShingle Effects of topical 2% cyclosporine A on the corneas of dogs subjected to lamellar keratoplasty with a graft of equine pericardium preserved in glycerin: clinical and morphological evaluation
Teixeira, Roseli Borges
cyclosporine
cornea
pericardium
vascularization
keratoplasty
dogs
ciclosporina
córnea
pericárdio
vascularização
ceratoplastia
cães
title_short Effects of topical 2% cyclosporine A on the corneas of dogs subjected to lamellar keratoplasty with a graft of equine pericardium preserved in glycerin: clinical and morphological evaluation
title_full Effects of topical 2% cyclosporine A on the corneas of dogs subjected to lamellar keratoplasty with a graft of equine pericardium preserved in glycerin: clinical and morphological evaluation
title_fullStr Effects of topical 2% cyclosporine A on the corneas of dogs subjected to lamellar keratoplasty with a graft of equine pericardium preserved in glycerin: clinical and morphological evaluation
title_full_unstemmed Effects of topical 2% cyclosporine A on the corneas of dogs subjected to lamellar keratoplasty with a graft of equine pericardium preserved in glycerin: clinical and morphological evaluation
title_sort Effects of topical 2% cyclosporine A on the corneas of dogs subjected to lamellar keratoplasty with a graft of equine pericardium preserved in glycerin: clinical and morphological evaluation
author Teixeira, Roseli Borges
author_facet Teixeira, Roseli Borges
Barros, Paulo Sergio De Moraes
Martins, Maria Cristina [UNIFESP]
author_role author
author2 Barros, Paulo Sergio De Moraes
Martins, Maria Cristina [UNIFESP]
author2_role author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Teixeira, Roseli Borges
Barros, Paulo Sergio De Moraes
Martins, Maria Cristina [UNIFESP]
dc.subject.por.fl_str_mv cyclosporine
cornea
pericardium
vascularization
keratoplasty
dogs
ciclosporina
córnea
pericárdio
vascularização
ceratoplastia
cães
topic cyclosporine
cornea
pericardium
vascularization
keratoplasty
dogs
ciclosporina
córnea
pericárdio
vascularização
ceratoplastia
cães
description The effects of topical 2% cyclosporine A on the cornea of dogs subjected to experimental lamellar keratoplasty with an equine pericardial graft were evaluated. Ten dogs were grouped to be evaluated 3, 7, 15, 30 and 60 days after surgery. Animals received bilateral grafts followed by the application of a 2% cyclosporine A ointment on the left eye (treated eye) and the ointment base on the right eye (control eye) twice a day. The ophthalmic evaluation showed profound bilateral blepharospasm, photophobia and a mucous secretion until the 7th day after surgery; corneal vascularization starting in the limbus was observed as early as the 3rd day in both eyes; opacification and vascularization were more intense in the treated cornea on days 15 and 30 after surgery. Vascularization was still evident on the 60th day, and looked similar in treated eyes and control eyes. The histologic evaluation showed a complete bilateral reepithelization and corneal vascularization three days after the surgery; intense vascularization in both eyes on days 15 and 30, that was much more pronounced in the treated cornea; and a bilateral predominance of polymorphonuclear cells until day 15, and mononuclear cells on day 30. Intact epithelium and stroma with new vessels, as well as graft absorption, in both eyes, were seen on the 60th day. The lamellar keratoplasty with equine pericardial graft was an effective model to study the inflammatory kinetics and corneal vascularization phenomenon. In this study, cyclosporine A did not inhibit corneal vascularization and it did not interfere in the corneal cicatricial process.
publishDate 2004
dc.date.none.fl_str_mv 2004-02-01
2015-06-14T13:30:19Z
2015-06-14T13:30:19Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0103-84782004000100020
Ciência Rural. Universidade Federal de Santa Maria, v. 34, n. 1, p. 131-138, 2004.
10.1590/S0103-84782004000100020
S0103-84782004000100020.pdf
0103-8478
S0103-84782004000100020
http://repositorio.unifesp.br/handle/11600/2015
url http://dx.doi.org/10.1590/S0103-84782004000100020
http://repositorio.unifesp.br/handle/11600/2015
identifier_str_mv Ciência Rural. Universidade Federal de Santa Maria, v. 34, n. 1, p. 131-138, 2004.
10.1590/S0103-84782004000100020
S0103-84782004000100020.pdf
0103-8478
S0103-84782004000100020
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Ciência Rural
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 131-138
application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
publisher.none.fl_str_mv Universidade Federal de Santa Maria
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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