Collagen and reticular fibers in left ventricular muscle in diabetic rats: Physical exercise prevents its changes?
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | https://repositorio.unifesp.br/handle/11600/33387 https://dx.doi.org/10.1016/j.tice.2010.11.001 |
Resumo: | Diabetic cardiomyopathy contributes to the high incidence of mortality in both types of diabetes. We aimed to investigate the histochemical aspects of collagen and reticular fibers in the cardiac muscle and evaluate the influence of physical exercise on these aspects. Wistar rats were divided in 4 groups: sedentary control (SC), trained control (TC), sedentary diabetic (SD) and trained diabetic (TD). Diabetes was induced with alloxan (35 mg/kg). Training program consisted of swimming 1 h/day with a load of 4.8% of body weight for TD and 5.2% for TC. during 8 weeks. At the end of the experiment, samples of the left ventricle were collected, fixed in Bouin and processed on historesin. Sections were stained with periodic acid of Schiff, picrosirius-hematoxylin and ammoniacal silver. the PAS technique shows that individuals of group SD presented more intense reaction that the other groups. Picrosirius-hematoxylin technique showed a possible deposition of collagen fibers in SD. the TD group presented a reaction a lot similar to the controls' for both techniques', showing a possible prevention of this deposition. These results indicate that physical exercises might have an important role on the prevention of some negative alterations caused by experimental diabetes. (C) 2010 Elsevier B.V. All rights reserved. |
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Castellar, AlexandreRemedio, Rafael NeodiniBarbosa, Rodrigo AvelairaGomes, Ricardo José [UNIFESP]Caetano, Flavio HenriqueSão Paulo State UnivUniversidade Federal de São Paulo (UNIFESP)2016-01-24T14:06:05Z2016-01-24T14:06:05Z2011-02-01Tissue & Cell. Edinburgh: Churchill Livingstone, v. 43, n. 1, p. 24-28, 2011.0040-8166https://repositorio.unifesp.br/handle/11600/33387https://dx.doi.org/10.1016/j.tice.2010.11.001WOS000288637500003.pdf10.1016/j.tice.2010.11.001WOS:000288637500003Diabetic cardiomyopathy contributes to the high incidence of mortality in both types of diabetes. We aimed to investigate the histochemical aspects of collagen and reticular fibers in the cardiac muscle and evaluate the influence of physical exercise on these aspects. Wistar rats were divided in 4 groups: sedentary control (SC), trained control (TC), sedentary diabetic (SD) and trained diabetic (TD). Diabetes was induced with alloxan (35 mg/kg). Training program consisted of swimming 1 h/day with a load of 4.8% of body weight for TD and 5.2% for TC. during 8 weeks. At the end of the experiment, samples of the left ventricle were collected, fixed in Bouin and processed on historesin. Sections were stained with periodic acid of Schiff, picrosirius-hematoxylin and ammoniacal silver. the PAS technique shows that individuals of group SD presented more intense reaction that the other groups. Picrosirius-hematoxylin technique showed a possible deposition of collagen fibers in SD. the TD group presented a reaction a lot similar to the controls' for both techniques', showing a possible prevention of this deposition. These results indicate that physical exercises might have an important role on the prevention of some negative alterations caused by experimental diabetes. (C) 2010 Elsevier B.V. All rights reserved.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)São Paulo State Univ, Dept Biol, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Biosci, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Biosci, São Paulo, BrazilWeb of Science24-28engChurchill LivingstoneTissue & CellDiabetes mellitusMyocardiumExerciseHistochemistryAlloxanCollagen and reticular fibers in left ventricular muscle in diabetic rats: Physical exercise prevents its changes?info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000288637500003.pdfapplication/pdf939925${dspace.ui.url}/bitstream/11600/33387/1/WOS000288637500003.pdf37a87bbe261a901fee5f79e8ea607aceMD51open accessTEXTWOS000288637500003.pdf.txtWOS000288637500003.pdf.txtExtracted texttext/plain24599${dspace.ui.url}/bitstream/11600/33387/6/WOS000288637500003.pdf.txte2b6805212603615e96b927ed2e148a8MD56open accessTHUMBNAILWOS000288637500003.pdf.jpgWOS000288637500003.pdf.jpgIM Thumbnailimage/jpeg7156${dspace.ui.url}/bitstream/11600/33387/8/WOS000288637500003.pdf.jpg1b74ef9a8f083071c6837ad83034471dMD58open access11600/333872023-06-05 19:16:00.606open accessoai:repositorio.unifesp.br:11600/33387Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-06-05T22:16Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Collagen and reticular fibers in left ventricular muscle in diabetic rats: Physical exercise prevents its changes? |
title |
Collagen and reticular fibers in left ventricular muscle in diabetic rats: Physical exercise prevents its changes? |
spellingShingle |
Collagen and reticular fibers in left ventricular muscle in diabetic rats: Physical exercise prevents its changes? Castellar, Alexandre Diabetes mellitus Myocardium Exercise Histochemistry Alloxan |
title_short |
Collagen and reticular fibers in left ventricular muscle in diabetic rats: Physical exercise prevents its changes? |
title_full |
Collagen and reticular fibers in left ventricular muscle in diabetic rats: Physical exercise prevents its changes? |
title_fullStr |
Collagen and reticular fibers in left ventricular muscle in diabetic rats: Physical exercise prevents its changes? |
title_full_unstemmed |
Collagen and reticular fibers in left ventricular muscle in diabetic rats: Physical exercise prevents its changes? |
title_sort |
Collagen and reticular fibers in left ventricular muscle in diabetic rats: Physical exercise prevents its changes? |
author |
Castellar, Alexandre |
author_facet |
Castellar, Alexandre Remedio, Rafael Neodini Barbosa, Rodrigo Avelaira Gomes, Ricardo José [UNIFESP] Caetano, Flavio Henrique |
author_role |
author |
author2 |
Remedio, Rafael Neodini Barbosa, Rodrigo Avelaira Gomes, Ricardo José [UNIFESP] Caetano, Flavio Henrique |
author2_role |
author author author author |
dc.contributor.institution.none.fl_str_mv |
São Paulo State Univ Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Castellar, Alexandre Remedio, Rafael Neodini Barbosa, Rodrigo Avelaira Gomes, Ricardo José [UNIFESP] Caetano, Flavio Henrique |
dc.subject.eng.fl_str_mv |
Diabetes mellitus Myocardium Exercise Histochemistry Alloxan |
topic |
Diabetes mellitus Myocardium Exercise Histochemistry Alloxan |
description |
Diabetic cardiomyopathy contributes to the high incidence of mortality in both types of diabetes. We aimed to investigate the histochemical aspects of collagen and reticular fibers in the cardiac muscle and evaluate the influence of physical exercise on these aspects. Wistar rats were divided in 4 groups: sedentary control (SC), trained control (TC), sedentary diabetic (SD) and trained diabetic (TD). Diabetes was induced with alloxan (35 mg/kg). Training program consisted of swimming 1 h/day with a load of 4.8% of body weight for TD and 5.2% for TC. during 8 weeks. At the end of the experiment, samples of the left ventricle were collected, fixed in Bouin and processed on historesin. Sections were stained with periodic acid of Schiff, picrosirius-hematoxylin and ammoniacal silver. the PAS technique shows that individuals of group SD presented more intense reaction that the other groups. Picrosirius-hematoxylin technique showed a possible deposition of collagen fibers in SD. the TD group presented a reaction a lot similar to the controls' for both techniques', showing a possible prevention of this deposition. These results indicate that physical exercises might have an important role on the prevention of some negative alterations caused by experimental diabetes. (C) 2010 Elsevier B.V. All rights reserved. |
publishDate |
2011 |
dc.date.issued.fl_str_mv |
2011-02-01 |
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2016-01-24T14:06:05Z |
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2016-01-24T14:06:05Z |
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info:eu-repo/semantics/publishedVersion |
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article |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
Tissue & Cell. Edinburgh: Churchill Livingstone, v. 43, n. 1, p. 24-28, 2011. |
dc.identifier.uri.fl_str_mv |
https://repositorio.unifesp.br/handle/11600/33387 https://dx.doi.org/10.1016/j.tice.2010.11.001 |
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0040-8166 |
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WOS000288637500003.pdf |
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10.1016/j.tice.2010.11.001 |
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WOS:000288637500003 |
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Tissue & Cell. Edinburgh: Churchill Livingstone, v. 43, n. 1, p. 24-28, 2011. 0040-8166 WOS000288637500003.pdf 10.1016/j.tice.2010.11.001 WOS:000288637500003 |
url |
https://repositorio.unifesp.br/handle/11600/33387 https://dx.doi.org/10.1016/j.tice.2010.11.001 |
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eng |
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Tissue & Cell |
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24-28 |
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Churchill Livingstone |
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Churchill Livingstone |
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