Walker 256 tumor growth suppression by crotoxin involves formyl peptide receptors and lipoxin a(4)

Detalhes bibliográficos
Autor(a) principal: Brigatte, Patricia
Data de Publicação: 2016
Outros Autores: Faiad, Odair Jorge, Ferreira Nocelli, Roberta Cornelio, Landgraf, Richardt G. [UNIFESP], Palma, Mario Sergio, Cury, Yara, Curi, Rui, Sampaio, Sandra Coccuzzo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1155/2016/2457532
http://repositorio.unifesp.br/handle/11600/49524
Resumo: We investigated the effects of Crotoxin (CTX), the main toxin of South American rattlesnake (Crotalus durissus terrificus) venom, on Walker 256 tumor growth, the pain symptoms associated (hyperalgesia and allodynia), and participation of endogenous lipoxin A(4). Treatment with CTX (s.c.), daily, for 5 days reduced tumor growth at the 5th day after injection of Walker 256 carcinoma cells into the plantar surface of adult rat hind paw. This observation was associated with inhibition of new blood vessel formation and decrease in blood vessel diameter. The treatment with CTX raised plasma concentrations of lipoxin A 4 and its natural analogue 15-epi-LXA(4) 4, an effect mediated by formyl peptide receptors (FPRs). In fact, the treatment with Boc-2, an inhibitor of FPRs, abolished the increase in plasma levels of these mediators triggered by CTX. The blockage of these receptors also abolished the inhibitory action of CTX on tumor growth and blood vessel formation and the decrease in blood vessel diameter. Together, the results herein presented demonstrate that CTX increases plasma concentrations of lipoxin A 4 and 15-epi-LXA 4, which might inhibit both tumor growth and formation of new vessels via FPRs.
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spelling Walker 256 tumor growth suppression by crotoxin involves formyl peptide receptors and lipoxin a(4)Crotalus-Durissus-TerrificusAspirin-Triggered LipoxinsArachidonic-AcidSnake-VenomAntiangiogenic TherapyCell-ProliferationLipid MediatorsAnalog SuppressCobra VenomCancer PainWe investigated the effects of Crotoxin (CTX), the main toxin of South American rattlesnake (Crotalus durissus terrificus) venom, on Walker 256 tumor growth, the pain symptoms associated (hyperalgesia and allodynia), and participation of endogenous lipoxin A(4). Treatment with CTX (s.c.), daily, for 5 days reduced tumor growth at the 5th day after injection of Walker 256 carcinoma cells into the plantar surface of adult rat hind paw. This observation was associated with inhibition of new blood vessel formation and decrease in blood vessel diameter. The treatment with CTX raised plasma concentrations of lipoxin A 4 and its natural analogue 15-epi-LXA(4) 4, an effect mediated by formyl peptide receptors (FPRs). In fact, the treatment with Boc-2, an inhibitor of FPRs, abolished the increase in plasma levels of these mediators triggered by CTX. The blockage of these receptors also abolished the inhibitory action of CTX on tumor growth and blood vessel formation and the decrease in blood vessel diameter. Together, the results herein presented demonstrate that CTX increases plasma concentrations of lipoxin A 4 and 15-epi-LXA 4, which might inhibit both tumor growth and formation of new vessels via FPRs.Special Laboratory of Pain and Signaling, Butantan Institute, Avenida Vital Brazil 1500, 05503-900 São Paulo, SP, BrazilCEIS/Department of Biology, Institute of Biosciences of Rio Claro, São Paulo State University (UNESP), Rio Claro, SP, BrazilLaboratory of Pathophysiology, Butantan Institute, Avenida Vital Brazil 1500, 05503-900 São Paulo, SP, BrazilDepartment of Natural Sciences, Mathematics and Education, Agricultural Sciences Center, Federal University of São Carlos, Rodovia Anhanguera Km 174, 13600-970 Araras, SP, BrazilLaboratory of Inflammation and Vascular Pharmacology, Federal University of São Paulo, Rua São Nicolau 210, 09913-030 Diadema, SP, BrazilDepartment of Physiology and Biophysics, Institute of Biomedical Sciences, University of São Paulo, Avenida Professor Lineu Prestes 1524, 05508-900 São Paulo, SP, BrazilDepartment of Pharmacology, Institute of Biomedical Sciences, University of São Paulo, Avenida Professor Lineu Prestes 1524, 05508-900 São Paulo, SP, BrazilLaboratory of Inflammation and Vascular Pharmacology, Federal University of São Paulo, Rua São Nicolau 210, 09913-030 Diadema, SP, BrazilWeb of ScienceCoordenacao de Aperfeicoamento de Pessoal de Nivel Superior (fellowship-CAPES)PAP (fellowship-Secretaria da Saude do Estado de Sao Paulo)FAPESP [07/52447-8]Guggenheim FoundationFAPESP: 07/52447-8Hindawi ltd2019-01-21T10:30:00Z2019-01-21T10:30:00Z2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://dx.doi.org/10.1155/2016/2457532Mediators Of Inflammation. London, 2016.10.1155/2016/2457532WOS000374851200001.pdf0962-9351http://repositorio.unifesp.br/handle/11600/49524WOS:000374851200001engMediators Of Inflammationinfo:eu-repo/semantics/openAccessBrigatte, PatriciaFaiad, Odair JorgeFerreira Nocelli, Roberta CornelioLandgraf, Richardt G. [UNIFESP]Palma, Mario SergioCury, YaraCuri, RuiSampaio, Sandra Coccuzzoreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-02T07:58:15Zoai:repositorio.unifesp.br/:11600/49524Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-02T07:58:15Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Walker 256 tumor growth suppression by crotoxin involves formyl peptide receptors and lipoxin a(4)
title Walker 256 tumor growth suppression by crotoxin involves formyl peptide receptors and lipoxin a(4)
spellingShingle Walker 256 tumor growth suppression by crotoxin involves formyl peptide receptors and lipoxin a(4)
Brigatte, Patricia
Crotalus-Durissus-Terrificus
Aspirin-Triggered Lipoxins
Arachidonic-Acid
Snake-Venom
Antiangiogenic Therapy
Cell-Proliferation
Lipid Mediators
Analog Suppress
Cobra Venom
Cancer Pain
title_short Walker 256 tumor growth suppression by crotoxin involves formyl peptide receptors and lipoxin a(4)
title_full Walker 256 tumor growth suppression by crotoxin involves formyl peptide receptors and lipoxin a(4)
title_fullStr Walker 256 tumor growth suppression by crotoxin involves formyl peptide receptors and lipoxin a(4)
title_full_unstemmed Walker 256 tumor growth suppression by crotoxin involves formyl peptide receptors and lipoxin a(4)
title_sort Walker 256 tumor growth suppression by crotoxin involves formyl peptide receptors and lipoxin a(4)
author Brigatte, Patricia
author_facet Brigatte, Patricia
Faiad, Odair Jorge
Ferreira Nocelli, Roberta Cornelio
Landgraf, Richardt G. [UNIFESP]
Palma, Mario Sergio
Cury, Yara
Curi, Rui
Sampaio, Sandra Coccuzzo
author_role author
author2 Faiad, Odair Jorge
Ferreira Nocelli, Roberta Cornelio
Landgraf, Richardt G. [UNIFESP]
Palma, Mario Sergio
Cury, Yara
Curi, Rui
Sampaio, Sandra Coccuzzo
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Brigatte, Patricia
Faiad, Odair Jorge
Ferreira Nocelli, Roberta Cornelio
Landgraf, Richardt G. [UNIFESP]
Palma, Mario Sergio
Cury, Yara
Curi, Rui
Sampaio, Sandra Coccuzzo
dc.subject.por.fl_str_mv Crotalus-Durissus-Terrificus
Aspirin-Triggered Lipoxins
Arachidonic-Acid
Snake-Venom
Antiangiogenic Therapy
Cell-Proliferation
Lipid Mediators
Analog Suppress
Cobra Venom
Cancer Pain
topic Crotalus-Durissus-Terrificus
Aspirin-Triggered Lipoxins
Arachidonic-Acid
Snake-Venom
Antiangiogenic Therapy
Cell-Proliferation
Lipid Mediators
Analog Suppress
Cobra Venom
Cancer Pain
description We investigated the effects of Crotoxin (CTX), the main toxin of South American rattlesnake (Crotalus durissus terrificus) venom, on Walker 256 tumor growth, the pain symptoms associated (hyperalgesia and allodynia), and participation of endogenous lipoxin A(4). Treatment with CTX (s.c.), daily, for 5 days reduced tumor growth at the 5th day after injection of Walker 256 carcinoma cells into the plantar surface of adult rat hind paw. This observation was associated with inhibition of new blood vessel formation and decrease in blood vessel diameter. The treatment with CTX raised plasma concentrations of lipoxin A 4 and its natural analogue 15-epi-LXA(4) 4, an effect mediated by formyl peptide receptors (FPRs). In fact, the treatment with Boc-2, an inhibitor of FPRs, abolished the increase in plasma levels of these mediators triggered by CTX. The blockage of these receptors also abolished the inhibitory action of CTX on tumor growth and blood vessel formation and the decrease in blood vessel diameter. Together, the results herein presented demonstrate that CTX increases plasma concentrations of lipoxin A 4 and 15-epi-LXA 4, which might inhibit both tumor growth and formation of new vessels via FPRs.
publishDate 2016
dc.date.none.fl_str_mv 2016
2019-01-21T10:30:00Z
2019-01-21T10:30:00Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1155/2016/2457532
Mediators Of Inflammation. London, 2016.
10.1155/2016/2457532
WOS000374851200001.pdf
0962-9351
http://repositorio.unifesp.br/handle/11600/49524
WOS:000374851200001
url http://dx.doi.org/10.1155/2016/2457532
http://repositorio.unifesp.br/handle/11600/49524
identifier_str_mv Mediators Of Inflammation. London, 2016.
10.1155/2016/2457532
WOS000374851200001.pdf
0962-9351
WOS:000374851200001
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Mediators Of Inflammation
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Hindawi ltd
publisher.none.fl_str_mv Hindawi ltd
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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