Walker 256 tumor growth suppression by crotoxin involves formyl peptide receptors and lipoxin a(4)
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://dx.doi.org/10.1155/2016/2457532 http://repositorio.unifesp.br/handle/11600/49524 |
Resumo: | We investigated the effects of Crotoxin (CTX), the main toxin of South American rattlesnake (Crotalus durissus terrificus) venom, on Walker 256 tumor growth, the pain symptoms associated (hyperalgesia and allodynia), and participation of endogenous lipoxin A(4). Treatment with CTX (s.c.), daily, for 5 days reduced tumor growth at the 5th day after injection of Walker 256 carcinoma cells into the plantar surface of adult rat hind paw. This observation was associated with inhibition of new blood vessel formation and decrease in blood vessel diameter. The treatment with CTX raised plasma concentrations of lipoxin A 4 and its natural analogue 15-epi-LXA(4) 4, an effect mediated by formyl peptide receptors (FPRs). In fact, the treatment with Boc-2, an inhibitor of FPRs, abolished the increase in plasma levels of these mediators triggered by CTX. The blockage of these receptors also abolished the inhibitory action of CTX on tumor growth and blood vessel formation and the decrease in blood vessel diameter. Together, the results herein presented demonstrate that CTX increases plasma concentrations of lipoxin A 4 and 15-epi-LXA 4, which might inhibit both tumor growth and formation of new vessels via FPRs. |
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Walker 256 tumor growth suppression by crotoxin involves formyl peptide receptors and lipoxin a(4)Crotalus-Durissus-TerrificusAspirin-Triggered LipoxinsArachidonic-AcidSnake-VenomAntiangiogenic TherapyCell-ProliferationLipid MediatorsAnalog SuppressCobra VenomCancer PainWe investigated the effects of Crotoxin (CTX), the main toxin of South American rattlesnake (Crotalus durissus terrificus) venom, on Walker 256 tumor growth, the pain symptoms associated (hyperalgesia and allodynia), and participation of endogenous lipoxin A(4). Treatment with CTX (s.c.), daily, for 5 days reduced tumor growth at the 5th day after injection of Walker 256 carcinoma cells into the plantar surface of adult rat hind paw. This observation was associated with inhibition of new blood vessel formation and decrease in blood vessel diameter. The treatment with CTX raised plasma concentrations of lipoxin A 4 and its natural analogue 15-epi-LXA(4) 4, an effect mediated by formyl peptide receptors (FPRs). In fact, the treatment with Boc-2, an inhibitor of FPRs, abolished the increase in plasma levels of these mediators triggered by CTX. The blockage of these receptors also abolished the inhibitory action of CTX on tumor growth and blood vessel formation and the decrease in blood vessel diameter. Together, the results herein presented demonstrate that CTX increases plasma concentrations of lipoxin A 4 and 15-epi-LXA 4, which might inhibit both tumor growth and formation of new vessels via FPRs.Special Laboratory of Pain and Signaling, Butantan Institute, Avenida Vital Brazil 1500, 05503-900 São Paulo, SP, BrazilCEIS/Department of Biology, Institute of Biosciences of Rio Claro, São Paulo State University (UNESP), Rio Claro, SP, BrazilLaboratory of Pathophysiology, Butantan Institute, Avenida Vital Brazil 1500, 05503-900 São Paulo, SP, BrazilDepartment of Natural Sciences, Mathematics and Education, Agricultural Sciences Center, Federal University of São Carlos, Rodovia Anhanguera Km 174, 13600-970 Araras, SP, BrazilLaboratory of Inflammation and Vascular Pharmacology, Federal University of São Paulo, Rua São Nicolau 210, 09913-030 Diadema, SP, BrazilDepartment of Physiology and Biophysics, Institute of Biomedical Sciences, University of São Paulo, Avenida Professor Lineu Prestes 1524, 05508-900 São Paulo, SP, BrazilDepartment of Pharmacology, Institute of Biomedical Sciences, University of São Paulo, Avenida Professor Lineu Prestes 1524, 05508-900 São Paulo, SP, BrazilLaboratory of Inflammation and Vascular Pharmacology, Federal University of São Paulo, Rua São Nicolau 210, 09913-030 Diadema, SP, BrazilWeb of ScienceCoordenacao de Aperfeicoamento de Pessoal de Nivel Superior (fellowship-CAPES)PAP (fellowship-Secretaria da Saude do Estado de Sao Paulo)FAPESP [07/52447-8]Guggenheim FoundationFAPESP: 07/52447-8Hindawi ltd2019-01-21T10:30:00Z2019-01-21T10:30:00Z2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://dx.doi.org/10.1155/2016/2457532Mediators Of Inflammation. London, 2016.10.1155/2016/2457532WOS000374851200001.pdf0962-9351http://repositorio.unifesp.br/handle/11600/49524WOS:000374851200001engMediators Of Inflammationinfo:eu-repo/semantics/openAccessBrigatte, PatriciaFaiad, Odair JorgeFerreira Nocelli, Roberta CornelioLandgraf, Richardt G. [UNIFESP]Palma, Mario SergioCury, YaraCuri, RuiSampaio, Sandra Coccuzzoreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-02T07:58:15Zoai:repositorio.unifesp.br/:11600/49524Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-02T07:58:15Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
Walker 256 tumor growth suppression by crotoxin involves formyl peptide receptors and lipoxin a(4) |
title |
Walker 256 tumor growth suppression by crotoxin involves formyl peptide receptors and lipoxin a(4) |
spellingShingle |
Walker 256 tumor growth suppression by crotoxin involves formyl peptide receptors and lipoxin a(4) Brigatte, Patricia Crotalus-Durissus-Terrificus Aspirin-Triggered Lipoxins Arachidonic-Acid Snake-Venom Antiangiogenic Therapy Cell-Proliferation Lipid Mediators Analog Suppress Cobra Venom Cancer Pain |
title_short |
Walker 256 tumor growth suppression by crotoxin involves formyl peptide receptors and lipoxin a(4) |
title_full |
Walker 256 tumor growth suppression by crotoxin involves formyl peptide receptors and lipoxin a(4) |
title_fullStr |
Walker 256 tumor growth suppression by crotoxin involves formyl peptide receptors and lipoxin a(4) |
title_full_unstemmed |
Walker 256 tumor growth suppression by crotoxin involves formyl peptide receptors and lipoxin a(4) |
title_sort |
Walker 256 tumor growth suppression by crotoxin involves formyl peptide receptors and lipoxin a(4) |
author |
Brigatte, Patricia |
author_facet |
Brigatte, Patricia Faiad, Odair Jorge Ferreira Nocelli, Roberta Cornelio Landgraf, Richardt G. [UNIFESP] Palma, Mario Sergio Cury, Yara Curi, Rui Sampaio, Sandra Coccuzzo |
author_role |
author |
author2 |
Faiad, Odair Jorge Ferreira Nocelli, Roberta Cornelio Landgraf, Richardt G. [UNIFESP] Palma, Mario Sergio Cury, Yara Curi, Rui Sampaio, Sandra Coccuzzo |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Brigatte, Patricia Faiad, Odair Jorge Ferreira Nocelli, Roberta Cornelio Landgraf, Richardt G. [UNIFESP] Palma, Mario Sergio Cury, Yara Curi, Rui Sampaio, Sandra Coccuzzo |
dc.subject.por.fl_str_mv |
Crotalus-Durissus-Terrificus Aspirin-Triggered Lipoxins Arachidonic-Acid Snake-Venom Antiangiogenic Therapy Cell-Proliferation Lipid Mediators Analog Suppress Cobra Venom Cancer Pain |
topic |
Crotalus-Durissus-Terrificus Aspirin-Triggered Lipoxins Arachidonic-Acid Snake-Venom Antiangiogenic Therapy Cell-Proliferation Lipid Mediators Analog Suppress Cobra Venom Cancer Pain |
description |
We investigated the effects of Crotoxin (CTX), the main toxin of South American rattlesnake (Crotalus durissus terrificus) venom, on Walker 256 tumor growth, the pain symptoms associated (hyperalgesia and allodynia), and participation of endogenous lipoxin A(4). Treatment with CTX (s.c.), daily, for 5 days reduced tumor growth at the 5th day after injection of Walker 256 carcinoma cells into the plantar surface of adult rat hind paw. This observation was associated with inhibition of new blood vessel formation and decrease in blood vessel diameter. The treatment with CTX raised plasma concentrations of lipoxin A 4 and its natural analogue 15-epi-LXA(4) 4, an effect mediated by formyl peptide receptors (FPRs). In fact, the treatment with Boc-2, an inhibitor of FPRs, abolished the increase in plasma levels of these mediators triggered by CTX. The blockage of these receptors also abolished the inhibitory action of CTX on tumor growth and blood vessel formation and the decrease in blood vessel diameter. Together, the results herein presented demonstrate that CTX increases plasma concentrations of lipoxin A 4 and 15-epi-LXA 4, which might inhibit both tumor growth and formation of new vessels via FPRs. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016 2019-01-21T10:30:00Z 2019-01-21T10:30:00Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1155/2016/2457532 Mediators Of Inflammation. London, 2016. 10.1155/2016/2457532 WOS000374851200001.pdf 0962-9351 http://repositorio.unifesp.br/handle/11600/49524 WOS:000374851200001 |
url |
http://dx.doi.org/10.1155/2016/2457532 http://repositorio.unifesp.br/handle/11600/49524 |
identifier_str_mv |
Mediators Of Inflammation. London, 2016. 10.1155/2016/2457532 WOS000374851200001.pdf 0962-9351 WOS:000374851200001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Mediators Of Inflammation |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Hindawi ltd |
publisher.none.fl_str_mv |
Hindawi ltd |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
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1814268390969180160 |