Assessment of the use of cryopreserved x freeze-dried amniotic membrane (AM) for reconstruction of ocular surface in rabbit model
Autor(a) principal: | |
---|---|
Data de Publicação: | 2008 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://dx.doi.org/10.1590/S0004-27492008000500011 http://repositorio.unifesp.br/handle/11600/30868 |
Resumo: | Purpose: To determine the efficacy of freeze-dried amniotic membrane (AM) for reconstruction of the ocular surface in rabbit eyes. Methods: the sterilized, freeze-dried amniotic membrane (lyophilized or FD-AM) is a preservative method that uses the drying by freezing process to maintain the AM well preserved for a long time even at room temperature. This paper is an experimental animal interventional study. One eye of each of 15 male New Zealand rabbits (1.5-3.0 kg) had the central cornea marked with a 6.0 mm trephine. the marked area was deepithelialized with a No. 15 blade. the denuded corneal surface was covered as follows: Group 1: cryopreserved AM (n=6); Group 2: freeze-dried AM (n=6); and Group 3: not covered (control group, n=3). the AM in group 1 and 2 and the periphery of the denuded area in group 3 were secured with continuous 10-0 nylon sutures. the clinical evaluation was made by a blinded observer and graded on a four-point scale (1=minimal, 4=marked) for conjunctival and ciliary hyperemia, eyelid edema, corneal neovascularization, corneal opacity and reepithelialization on postoperative (PO) days 1, 7 and 30. After PO day 30, the rabbits were euthanized and their corneas were sent for histopathological and ultrastructural analysis to evaluate tissue inflammation, reepithelialization, and basement membrane integrity. Results: Two eyes in group 2 had a corneal infection and were excluded from the analysis. No statistically significant differences among the three groups were found (p>0.05) regarding the clinical evaluation on 1(st), 7(th) and 30(th) PO days. On transmission electron microscopy, the basement membrane in lyophilized and control groups was more continuous and homogeneous than in the glycerol group. Conclusions: the freeze-drying method seems to be a good option to preserve human amniotic membrane to be used in ocular surface reconstruction. This preservative method reduces the preservation costs and may enhance the use of AM, facilitating its storage and transport. |
id |
UFSP_3f6d68c1013d07fe2dc0d6298d030715 |
---|---|
oai_identifier_str |
oai:repositorio.unifesp.br/:11600/30868 |
network_acronym_str |
UFSP |
network_name_str |
Repositório Institucional da UNIFESP |
repository_id_str |
3465 |
spelling |
Assessment of the use of cryopreserved x freeze-dried amniotic membrane (AM) for reconstruction of ocular surface in rabbit modelAmnion/transplantationFreeze-dryingCryopreservationTissue preservation/methodsRabbitsPurpose: To determine the efficacy of freeze-dried amniotic membrane (AM) for reconstruction of the ocular surface in rabbit eyes. Methods: the sterilized, freeze-dried amniotic membrane (lyophilized or FD-AM) is a preservative method that uses the drying by freezing process to maintain the AM well preserved for a long time even at room temperature. This paper is an experimental animal interventional study. One eye of each of 15 male New Zealand rabbits (1.5-3.0 kg) had the central cornea marked with a 6.0 mm trephine. the marked area was deepithelialized with a No. 15 blade. the denuded corneal surface was covered as follows: Group 1: cryopreserved AM (n=6); Group 2: freeze-dried AM (n=6); and Group 3: not covered (control group, n=3). the AM in group 1 and 2 and the periphery of the denuded area in group 3 were secured with continuous 10-0 nylon sutures. the clinical evaluation was made by a blinded observer and graded on a four-point scale (1=minimal, 4=marked) for conjunctival and ciliary hyperemia, eyelid edema, corneal neovascularization, corneal opacity and reepithelialization on postoperative (PO) days 1, 7 and 30. After PO day 30, the rabbits were euthanized and their corneas were sent for histopathological and ultrastructural analysis to evaluate tissue inflammation, reepithelialization, and basement membrane integrity. Results: Two eyes in group 2 had a corneal infection and were excluded from the analysis. No statistically significant differences among the three groups were found (p>0.05) regarding the clinical evaluation on 1(st), 7(th) and 30(th) PO days. On transmission electron microscopy, the basement membrane in lyophilized and control groups was more continuous and homogeneous than in the glycerol group. Conclusions: the freeze-drying method seems to be a good option to preserve human amniotic membrane to be used in ocular surface reconstruction. This preservative method reduces the preservation costs and may enhance the use of AM, facilitating its storage and transport.Universidade Federal de São Paulo, Dept Ophthalmol, BR-04023062 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Ophthalmol, BR-04023062 São Paulo, BrazilWeb of ScienceConsel Brasil OftalmologiaUniversidade Federal de São Paulo (UNIFESP)Libera, Rodrigo Doyle [UNIFESP]Melo, Gustavo Barreto deLima, Acacio de SouzaHaapalainen, Edna Freymuller [UNIFESP]Cristovam, PriscilaPereira Gomes, Jose Alvaro2016-01-24T13:51:38Z2016-01-24T13:51:38Z2008-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion669-673application/pdfhttp://dx.doi.org/10.1590/S0004-27492008000500011Arquivos Brasileiros de Oftalmologia. São Paulo: Consel Brasil Oftalmologia, v. 71, n. 5, p. 669-673, 2008.10.1590/S0004-27492008000500011S0004-27492008000500011.pdf0004-2749S0004-27492008000500011http://repositorio.unifesp.br/handle/11600/30868WOS:000273039800011engArquivos Brasileiros de Oftalmologiainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-07-30T11:51:50Zoai:repositorio.unifesp.br/:11600/30868Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-07-30T11:51:50Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
Assessment of the use of cryopreserved x freeze-dried amniotic membrane (AM) for reconstruction of ocular surface in rabbit model |
title |
Assessment of the use of cryopreserved x freeze-dried amniotic membrane (AM) for reconstruction of ocular surface in rabbit model |
spellingShingle |
Assessment of the use of cryopreserved x freeze-dried amniotic membrane (AM) for reconstruction of ocular surface in rabbit model Libera, Rodrigo Doyle [UNIFESP] Amnion/transplantation Freeze-drying Cryopreservation Tissue preservation/methods Rabbits |
title_short |
Assessment of the use of cryopreserved x freeze-dried amniotic membrane (AM) for reconstruction of ocular surface in rabbit model |
title_full |
Assessment of the use of cryopreserved x freeze-dried amniotic membrane (AM) for reconstruction of ocular surface in rabbit model |
title_fullStr |
Assessment of the use of cryopreserved x freeze-dried amniotic membrane (AM) for reconstruction of ocular surface in rabbit model |
title_full_unstemmed |
Assessment of the use of cryopreserved x freeze-dried amniotic membrane (AM) for reconstruction of ocular surface in rabbit model |
title_sort |
Assessment of the use of cryopreserved x freeze-dried amniotic membrane (AM) for reconstruction of ocular surface in rabbit model |
author |
Libera, Rodrigo Doyle [UNIFESP] |
author_facet |
Libera, Rodrigo Doyle [UNIFESP] Melo, Gustavo Barreto de Lima, Acacio de Souza Haapalainen, Edna Freymuller [UNIFESP] Cristovam, Priscila Pereira Gomes, Jose Alvaro |
author_role |
author |
author2 |
Melo, Gustavo Barreto de Lima, Acacio de Souza Haapalainen, Edna Freymuller [UNIFESP] Cristovam, Priscila Pereira Gomes, Jose Alvaro |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Libera, Rodrigo Doyle [UNIFESP] Melo, Gustavo Barreto de Lima, Acacio de Souza Haapalainen, Edna Freymuller [UNIFESP] Cristovam, Priscila Pereira Gomes, Jose Alvaro |
dc.subject.por.fl_str_mv |
Amnion/transplantation Freeze-drying Cryopreservation Tissue preservation/methods Rabbits |
topic |
Amnion/transplantation Freeze-drying Cryopreservation Tissue preservation/methods Rabbits |
description |
Purpose: To determine the efficacy of freeze-dried amniotic membrane (AM) for reconstruction of the ocular surface in rabbit eyes. Methods: the sterilized, freeze-dried amniotic membrane (lyophilized or FD-AM) is a preservative method that uses the drying by freezing process to maintain the AM well preserved for a long time even at room temperature. This paper is an experimental animal interventional study. One eye of each of 15 male New Zealand rabbits (1.5-3.0 kg) had the central cornea marked with a 6.0 mm trephine. the marked area was deepithelialized with a No. 15 blade. the denuded corneal surface was covered as follows: Group 1: cryopreserved AM (n=6); Group 2: freeze-dried AM (n=6); and Group 3: not covered (control group, n=3). the AM in group 1 and 2 and the periphery of the denuded area in group 3 were secured with continuous 10-0 nylon sutures. the clinical evaluation was made by a blinded observer and graded on a four-point scale (1=minimal, 4=marked) for conjunctival and ciliary hyperemia, eyelid edema, corneal neovascularization, corneal opacity and reepithelialization on postoperative (PO) days 1, 7 and 30. After PO day 30, the rabbits were euthanized and their corneas were sent for histopathological and ultrastructural analysis to evaluate tissue inflammation, reepithelialization, and basement membrane integrity. Results: Two eyes in group 2 had a corneal infection and were excluded from the analysis. No statistically significant differences among the three groups were found (p>0.05) regarding the clinical evaluation on 1(st), 7(th) and 30(th) PO days. On transmission electron microscopy, the basement membrane in lyophilized and control groups was more continuous and homogeneous than in the glycerol group. Conclusions: the freeze-drying method seems to be a good option to preserve human amniotic membrane to be used in ocular surface reconstruction. This preservative method reduces the preservation costs and may enhance the use of AM, facilitating its storage and transport. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-09-01 2016-01-24T13:51:38Z 2016-01-24T13:51:38Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0004-27492008000500011 Arquivos Brasileiros de Oftalmologia. São Paulo: Consel Brasil Oftalmologia, v. 71, n. 5, p. 669-673, 2008. 10.1590/S0004-27492008000500011 S0004-27492008000500011.pdf 0004-2749 S0004-27492008000500011 http://repositorio.unifesp.br/handle/11600/30868 WOS:000273039800011 |
url |
http://dx.doi.org/10.1590/S0004-27492008000500011 http://repositorio.unifesp.br/handle/11600/30868 |
identifier_str_mv |
Arquivos Brasileiros de Oftalmologia. São Paulo: Consel Brasil Oftalmologia, v. 71, n. 5, p. 669-673, 2008. 10.1590/S0004-27492008000500011 S0004-27492008000500011.pdf 0004-2749 S0004-27492008000500011 WOS:000273039800011 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Arquivos Brasileiros de Oftalmologia |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
669-673 application/pdf |
dc.publisher.none.fl_str_mv |
Consel Brasil Oftalmologia |
publisher.none.fl_str_mv |
Consel Brasil Oftalmologia |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
_version_ |
1814268423871397888 |