Assessment of the use of cryopreserved x freeze-dried amniotic membrane (AM) for reconstruction of ocular surface in rabbit model

Detalhes bibliográficos
Autor(a) principal: Libera, Rodrigo Doyle [UNIFESP]
Data de Publicação: 2008
Outros Autores: Melo, Gustavo Barreto de, Lima, Acacio de Souza, Haapalainen, Edna Freymuller [UNIFESP], Cristovam, Priscila, Pereira Gomes, Jose Alvaro
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1590/S0004-27492008000500011
http://repositorio.unifesp.br/handle/11600/30868
Resumo: Purpose: To determine the efficacy of freeze-dried amniotic membrane (AM) for reconstruction of the ocular surface in rabbit eyes. Methods: the sterilized, freeze-dried amniotic membrane (lyophilized or FD-AM) is a preservative method that uses the drying by freezing process to maintain the AM well preserved for a long time even at room temperature. This paper is an experimental animal interventional study. One eye of each of 15 male New Zealand rabbits (1.5-3.0 kg) had the central cornea marked with a 6.0 mm trephine. the marked area was deepithelialized with a No. 15 blade. the denuded corneal surface was covered as follows: Group 1: cryopreserved AM (n=6); Group 2: freeze-dried AM (n=6); and Group 3: not covered (control group, n=3). the AM in group 1 and 2 and the periphery of the denuded area in group 3 were secured with continuous 10-0 nylon sutures. the clinical evaluation was made by a blinded observer and graded on a four-point scale (1=minimal, 4=marked) for conjunctival and ciliary hyperemia, eyelid edema, corneal neovascularization, corneal opacity and reepithelialization on postoperative (PO) days 1, 7 and 30. After PO day 30, the rabbits were euthanized and their corneas were sent for histopathological and ultrastructural analysis to evaluate tissue inflammation, reepithelialization, and basement membrane integrity. Results: Two eyes in group 2 had a corneal infection and were excluded from the analysis. No statistically significant differences among the three groups were found (p>0.05) regarding the clinical evaluation on 1(st), 7(th) and 30(th) PO days. On transmission electron microscopy, the basement membrane in lyophilized and control groups was more continuous and homogeneous than in the glycerol group. Conclusions: the freeze-drying method seems to be a good option to preserve human amniotic membrane to be used in ocular surface reconstruction. This preservative method reduces the preservation costs and may enhance the use of AM, facilitating its storage and transport.
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spelling Assessment of the use of cryopreserved x freeze-dried amniotic membrane (AM) for reconstruction of ocular surface in rabbit modelAmnion/transplantationFreeze-dryingCryopreservationTissue preservation/methodsRabbitsPurpose: To determine the efficacy of freeze-dried amniotic membrane (AM) for reconstruction of the ocular surface in rabbit eyes. Methods: the sterilized, freeze-dried amniotic membrane (lyophilized or FD-AM) is a preservative method that uses the drying by freezing process to maintain the AM well preserved for a long time even at room temperature. This paper is an experimental animal interventional study. One eye of each of 15 male New Zealand rabbits (1.5-3.0 kg) had the central cornea marked with a 6.0 mm trephine. the marked area was deepithelialized with a No. 15 blade. the denuded corneal surface was covered as follows: Group 1: cryopreserved AM (n=6); Group 2: freeze-dried AM (n=6); and Group 3: not covered (control group, n=3). the AM in group 1 and 2 and the periphery of the denuded area in group 3 were secured with continuous 10-0 nylon sutures. the clinical evaluation was made by a blinded observer and graded on a four-point scale (1=minimal, 4=marked) for conjunctival and ciliary hyperemia, eyelid edema, corneal neovascularization, corneal opacity and reepithelialization on postoperative (PO) days 1, 7 and 30. After PO day 30, the rabbits were euthanized and their corneas were sent for histopathological and ultrastructural analysis to evaluate tissue inflammation, reepithelialization, and basement membrane integrity. Results: Two eyes in group 2 had a corneal infection and were excluded from the analysis. No statistically significant differences among the three groups were found (p>0.05) regarding the clinical evaluation on 1(st), 7(th) and 30(th) PO days. On transmission electron microscopy, the basement membrane in lyophilized and control groups was more continuous and homogeneous than in the glycerol group. Conclusions: the freeze-drying method seems to be a good option to preserve human amniotic membrane to be used in ocular surface reconstruction. This preservative method reduces the preservation costs and may enhance the use of AM, facilitating its storage and transport.Universidade Federal de São Paulo, Dept Ophthalmol, BR-04023062 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Ophthalmol, BR-04023062 São Paulo, BrazilWeb of ScienceConsel Brasil OftalmologiaUniversidade Federal de São Paulo (UNIFESP)Libera, Rodrigo Doyle [UNIFESP]Melo, Gustavo Barreto deLima, Acacio de SouzaHaapalainen, Edna Freymuller [UNIFESP]Cristovam, PriscilaPereira Gomes, Jose Alvaro2016-01-24T13:51:38Z2016-01-24T13:51:38Z2008-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion669-673application/pdfhttp://dx.doi.org/10.1590/S0004-27492008000500011Arquivos Brasileiros de Oftalmologia. São Paulo: Consel Brasil Oftalmologia, v. 71, n. 5, p. 669-673, 2008.10.1590/S0004-27492008000500011S0004-27492008000500011.pdf0004-2749S0004-27492008000500011http://repositorio.unifesp.br/handle/11600/30868WOS:000273039800011engArquivos Brasileiros de Oftalmologiainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-07-30T11:51:50Zoai:repositorio.unifesp.br/:11600/30868Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-07-30T11:51:50Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Assessment of the use of cryopreserved x freeze-dried amniotic membrane (AM) for reconstruction of ocular surface in rabbit model
title Assessment of the use of cryopreserved x freeze-dried amniotic membrane (AM) for reconstruction of ocular surface in rabbit model
spellingShingle Assessment of the use of cryopreserved x freeze-dried amniotic membrane (AM) for reconstruction of ocular surface in rabbit model
Libera, Rodrigo Doyle [UNIFESP]
Amnion/transplantation
Freeze-drying
Cryopreservation
Tissue preservation/methods
Rabbits
title_short Assessment of the use of cryopreserved x freeze-dried amniotic membrane (AM) for reconstruction of ocular surface in rabbit model
title_full Assessment of the use of cryopreserved x freeze-dried amniotic membrane (AM) for reconstruction of ocular surface in rabbit model
title_fullStr Assessment of the use of cryopreserved x freeze-dried amniotic membrane (AM) for reconstruction of ocular surface in rabbit model
title_full_unstemmed Assessment of the use of cryopreserved x freeze-dried amniotic membrane (AM) for reconstruction of ocular surface in rabbit model
title_sort Assessment of the use of cryopreserved x freeze-dried amniotic membrane (AM) for reconstruction of ocular surface in rabbit model
author Libera, Rodrigo Doyle [UNIFESP]
author_facet Libera, Rodrigo Doyle [UNIFESP]
Melo, Gustavo Barreto de
Lima, Acacio de Souza
Haapalainen, Edna Freymuller [UNIFESP]
Cristovam, Priscila
Pereira Gomes, Jose Alvaro
author_role author
author2 Melo, Gustavo Barreto de
Lima, Acacio de Souza
Haapalainen, Edna Freymuller [UNIFESP]
Cristovam, Priscila
Pereira Gomes, Jose Alvaro
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Libera, Rodrigo Doyle [UNIFESP]
Melo, Gustavo Barreto de
Lima, Acacio de Souza
Haapalainen, Edna Freymuller [UNIFESP]
Cristovam, Priscila
Pereira Gomes, Jose Alvaro
dc.subject.por.fl_str_mv Amnion/transplantation
Freeze-drying
Cryopreservation
Tissue preservation/methods
Rabbits
topic Amnion/transplantation
Freeze-drying
Cryopreservation
Tissue preservation/methods
Rabbits
description Purpose: To determine the efficacy of freeze-dried amniotic membrane (AM) for reconstruction of the ocular surface in rabbit eyes. Methods: the sterilized, freeze-dried amniotic membrane (lyophilized or FD-AM) is a preservative method that uses the drying by freezing process to maintain the AM well preserved for a long time even at room temperature. This paper is an experimental animal interventional study. One eye of each of 15 male New Zealand rabbits (1.5-3.0 kg) had the central cornea marked with a 6.0 mm trephine. the marked area was deepithelialized with a No. 15 blade. the denuded corneal surface was covered as follows: Group 1: cryopreserved AM (n=6); Group 2: freeze-dried AM (n=6); and Group 3: not covered (control group, n=3). the AM in group 1 and 2 and the periphery of the denuded area in group 3 were secured with continuous 10-0 nylon sutures. the clinical evaluation was made by a blinded observer and graded on a four-point scale (1=minimal, 4=marked) for conjunctival and ciliary hyperemia, eyelid edema, corneal neovascularization, corneal opacity and reepithelialization on postoperative (PO) days 1, 7 and 30. After PO day 30, the rabbits were euthanized and their corneas were sent for histopathological and ultrastructural analysis to evaluate tissue inflammation, reepithelialization, and basement membrane integrity. Results: Two eyes in group 2 had a corneal infection and were excluded from the analysis. No statistically significant differences among the three groups were found (p>0.05) regarding the clinical evaluation on 1(st), 7(th) and 30(th) PO days. On transmission electron microscopy, the basement membrane in lyophilized and control groups was more continuous and homogeneous than in the glycerol group. Conclusions: the freeze-drying method seems to be a good option to preserve human amniotic membrane to be used in ocular surface reconstruction. This preservative method reduces the preservation costs and may enhance the use of AM, facilitating its storage and transport.
publishDate 2008
dc.date.none.fl_str_mv 2008-09-01
2016-01-24T13:51:38Z
2016-01-24T13:51:38Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0004-27492008000500011
Arquivos Brasileiros de Oftalmologia. São Paulo: Consel Brasil Oftalmologia, v. 71, n. 5, p. 669-673, 2008.
10.1590/S0004-27492008000500011
S0004-27492008000500011.pdf
0004-2749
S0004-27492008000500011
http://repositorio.unifesp.br/handle/11600/30868
WOS:000273039800011
url http://dx.doi.org/10.1590/S0004-27492008000500011
http://repositorio.unifesp.br/handle/11600/30868
identifier_str_mv Arquivos Brasileiros de Oftalmologia. São Paulo: Consel Brasil Oftalmologia, v. 71, n. 5, p. 669-673, 2008.
10.1590/S0004-27492008000500011
S0004-27492008000500011.pdf
0004-2749
S0004-27492008000500011
WOS:000273039800011
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Arquivos Brasileiros de Oftalmologia
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 669-673
application/pdf
dc.publisher.none.fl_str_mv Consel Brasil Oftalmologia
publisher.none.fl_str_mv Consel Brasil Oftalmologia
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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