Disruption of beta3 adrenergic receptor increases susceptibility to DIO in mouse
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2016 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UNIFESP |
| dARK ID: | ark:/48912/001300000z13b |
| Texto Completo: | http://dx.doi.org/10.1530/JOE-16-0199 https://repositorio.unifesp.br/handle/11600/56682 |
Resumo: | The brown adipose tissue (BAT) mediates adaptive changes in metabolic rate by responding to the sympathetic nervous system through beta-adrenergic receptors (AR). Here, we wished to define the role played by the AR beta(3) isoform in this process. This study focused on the AR beta(3) knockout mice (AR beta 3KO), including responsiveness to cold exposure, diet-induced obesity, intolerance to glucose, dyslipidaemia and lipolysis in white adipose tissue (WAT). AR beta 3KO mice defend core temperature during cold exposure (4 degrees C for 5h), with faster BAT thermal response to norepinephrine (NE) infusion when compared with wild-type (WT) mice. Despite normal BAT thermogenesis, AR beta 3KO mice kept on a high-fat diet (HFD |
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Disruption of beta3 adrenergic receptor increases susceptibility to DIO in mouseobesitylipolysisadaptive thermogenesisbrown adipose tissuebeta(3) adrenergic receptorThe brown adipose tissue (BAT) mediates adaptive changes in metabolic rate by responding to the sympathetic nervous system through beta-adrenergic receptors (AR). Here, we wished to define the role played by the AR beta(3) isoform in this process. This study focused on the AR beta(3) knockout mice (AR beta 3KO), including responsiveness to cold exposure, diet-induced obesity, intolerance to glucose, dyslipidaemia and lipolysis in white adipose tissue (WAT). AR beta 3KO mice defend core temperature during cold exposure (4 degrees C for 5h), with faster BAT thermal response to norepinephrine (NE) infusion when compared with wild-type (WT) mice. Despite normal BAT thermogenesis, AR beta 3KO mice kept on a high-fat diet (HFD40% fat) for 8 weeks exhibited greater susceptibility to diet-induced obesity, markedly increased epididymal adipocyte area with clear signs of inflammation. The HFD-induced glucose intolerance was similar in both groups but serum hypertriglyceridemia and hypercholesterolemia were less intense in AR beta 3KO animals when compared with WT controls. Isoproterenol-induced lipolysis in isolated white adipocytes as assessed by glycerol release was significantly impaired in AR beta 3KO animals despite normal expression of key proteins involved in lipid metabolism. In conclusion, AR beta(3) inactivation does not affect BAT thermogenesis but increases susceptibility to diet-induced obesity by dampening WAT lipolytic response to adrenergic stimulation.Univ Prebiteriana Mackenzie, Ctr Biol & Hlth Sci, Sao Paulo, SP, BrazilUniv Fed Sao Paulo, Dept Translat Med, EPM, Sao Paulo, SP, BrazilUniv Sao Paulo, Expt Pathophysiol Dept, Fac Med, BR-05508 Sao Paulo, SP, BrazilUniv Sao Paulo, Sch Arts Sci & Humanities, Sao Paulo, SP, BrazilSch Med Sci, Dept Pathol, Sao Paulo, SP, BrazilUniv Mogi das Cruzes, Integrated Grp Biotechnol, Lab Adipose Tissue Biol, Mogi Das Cruzes, SP, BrazilRush Univ & Med Ctr, Dept Internal Med, Div Endocrinol & Metab, Chicago, IL USADepartment of Translational Medicine, EPM, Universidade Federal de São Paulo (UNIFESP), Sao Paulo, SP, BrazilWeb of ScienceFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)FAPESP: 2009/50353-1FAPESP: 2015/19259-0Bioscientifica Ltd2020-07-31T12:47:14Z2020-07-31T12:47:14Z2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion259-269http://dx.doi.org/10.1530/JOE-16-0199Journal Of Endocrinology. Bristol, v. 231, n. 3, p. 259-269, 2016.10.1530/JOE-16-01990022-0795https://repositorio.unifesp.br/handle/11600/56682WOS:000387982200009ark:/48912/001300000z13bengJournal Of EndocrinologyBristolinfo:eu-repo/semantics/openAccessPreite, Nailliw Zanini [UNIFESP]Nascimento, Bruna Pascarelli Pedrico do [UNIFESP]Muller, Cynthia R.Americo, Anna Laura V.Higa, Talita S.Evangelista, Fabiana S.Lancellotti, Carmen L.Henriques, Felipe dos SantosBatista, Miguel Luiz, Jr.Bianco, Antonio C.Ribeiro, Miriam O.reponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2022-02-10T21:18:19Zoai:repositorio.unifesp.br/:11600/56682Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-12-11T20:44:27.413595Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
| dc.title.none.fl_str_mv |
Disruption of beta3 adrenergic receptor increases susceptibility to DIO in mouse |
| title |
Disruption of beta3 adrenergic receptor increases susceptibility to DIO in mouse |
| spellingShingle |
Disruption of beta3 adrenergic receptor increases susceptibility to DIO in mouse Preite, Nailliw Zanini [UNIFESP] obesity lipolysis adaptive thermogenesis brown adipose tissue beta(3) adrenergic receptor |
| title_short |
Disruption of beta3 adrenergic receptor increases susceptibility to DIO in mouse |
| title_full |
Disruption of beta3 adrenergic receptor increases susceptibility to DIO in mouse |
| title_fullStr |
Disruption of beta3 adrenergic receptor increases susceptibility to DIO in mouse |
| title_full_unstemmed |
Disruption of beta3 adrenergic receptor increases susceptibility to DIO in mouse |
| title_sort |
Disruption of beta3 adrenergic receptor increases susceptibility to DIO in mouse |
| author |
Preite, Nailliw Zanini [UNIFESP] |
| author_facet |
Preite, Nailliw Zanini [UNIFESP] Nascimento, Bruna Pascarelli Pedrico do [UNIFESP] Muller, Cynthia R. Americo, Anna Laura V. Higa, Talita S. Evangelista, Fabiana S. Lancellotti, Carmen L. Henriques, Felipe dos Santos Batista, Miguel Luiz, Jr. Bianco, Antonio C. Ribeiro, Miriam O. |
| author_role |
author |
| author2 |
Nascimento, Bruna Pascarelli Pedrico do [UNIFESP] Muller, Cynthia R. Americo, Anna Laura V. Higa, Talita S. Evangelista, Fabiana S. Lancellotti, Carmen L. Henriques, Felipe dos Santos Batista, Miguel Luiz, Jr. Bianco, Antonio C. Ribeiro, Miriam O. |
| author2_role |
author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Preite, Nailliw Zanini [UNIFESP] Nascimento, Bruna Pascarelli Pedrico do [UNIFESP] Muller, Cynthia R. Americo, Anna Laura V. Higa, Talita S. Evangelista, Fabiana S. Lancellotti, Carmen L. Henriques, Felipe dos Santos Batista, Miguel Luiz, Jr. Bianco, Antonio C. Ribeiro, Miriam O. |
| dc.subject.por.fl_str_mv |
obesity lipolysis adaptive thermogenesis brown adipose tissue beta(3) adrenergic receptor |
| topic |
obesity lipolysis adaptive thermogenesis brown adipose tissue beta(3) adrenergic receptor |
| description |
The brown adipose tissue (BAT) mediates adaptive changes in metabolic rate by responding to the sympathetic nervous system through beta-adrenergic receptors (AR). Here, we wished to define the role played by the AR beta(3) isoform in this process. This study focused on the AR beta(3) knockout mice (AR beta 3KO), including responsiveness to cold exposure, diet-induced obesity, intolerance to glucose, dyslipidaemia and lipolysis in white adipose tissue (WAT). AR beta 3KO mice defend core temperature during cold exposure (4 degrees C for 5h), with faster BAT thermal response to norepinephrine (NE) infusion when compared with wild-type (WT) mice. Despite normal BAT thermogenesis, AR beta 3KO mice kept on a high-fat diet (HFD |
| publishDate |
2016 |
| dc.date.none.fl_str_mv |
2016 2020-07-31T12:47:14Z 2020-07-31T12:47:14Z |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1530/JOE-16-0199 Journal Of Endocrinology. Bristol, v. 231, n. 3, p. 259-269, 2016. 10.1530/JOE-16-0199 0022-0795 https://repositorio.unifesp.br/handle/11600/56682 WOS:000387982200009 |
| dc.identifier.dark.fl_str_mv |
ark:/48912/001300000z13b |
| url |
http://dx.doi.org/10.1530/JOE-16-0199 https://repositorio.unifesp.br/handle/11600/56682 |
| identifier_str_mv |
Journal Of Endocrinology. Bristol, v. 231, n. 3, p. 259-269, 2016. 10.1530/JOE-16-0199 0022-0795 WOS:000387982200009 ark:/48912/001300000z13b |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
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Journal Of Endocrinology |
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info:eu-repo/semantics/openAccess |
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openAccess |
| dc.format.none.fl_str_mv |
259-269 |
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Bristol |
| dc.publisher.none.fl_str_mv |
Bioscientifica Ltd |
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Bioscientifica Ltd |
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reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
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Universidade Federal de São Paulo (UNIFESP) |
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UNIFESP |
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UNIFESP |
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Repositório Institucional da UNIFESP |
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Repositório Institucional da UNIFESP |
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Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
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biblioteca.csp@unifesp.br |
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1818602536974680064 |