Proteolytic processed form of CXCL12 abolishes migration and induces apoptosis in neural stem cells in vitro
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://dx.doi.org/10.1016/j.scr.2017.05.013 https://repositorio.unifesp.br/handle/11600/53576 |
Resumo: | The subventricular zone (SVZ) of the adult mammalian brain hosts full potential neural stem cells (NSCs). NSCs are able to respond to extracellular signals in the brain, amplifying the pool of progenitor cells and giving rise to neuroblasts that showability to migrate towards an injury site. These signals can come fromvascular system, cerebrospinal fluid, glial cells, or projections of neurons in adjoining regions. CXCL12, a chemokine secreted after brain injury, reaches the SVZ in a gradient manner and drives neuroblasts towards the lesion area. Among many other molecules, matrix metalloproteinase 2 and 9 (MMP-2/9) are also released during brain injury. MMP-2/9 can cleave CXCL12 generating a new molecule, CXCL12(5-67), and its effects on NSCs viability is not well described. Here we produced recombinant CXCL12 and CXCL12(5-67) and evaluated their effect inmurine adultNSCsmigration and survival in vitro. We showed CXCL12(5-67) does not promote NSCsmigration, but does induce cell death. The NSC death induced by CXCL12(5-67) involves caspases 9 and 3/7 activation, implying the intrinsic apoptotic pathway in this phenomenon. Our evidences in vitromake CXCL12(5-67) and its receptor potential candidates for brain injuries and neurodegeneration studies. (C) 2017 The Author(s). Published by Elsevier B.V. |
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Adelita, Tais [UNIFESP]Stilhano, Roberta Sessa [UNIFESP]Han, SangWon [UNIFESP]Justo, Giselle Zenker [UNIFESP]Porcionatto, Marimelia [UNIFESP]2020-06-26T16:30:30Z2020-06-26T16:30:30Z2017http://dx.doi.org/10.1016/j.scr.2017.05.013Stem Cell Research. Amsterdam, v. 22, p. 61-69, 2017.1873-5061https://repositorio.unifesp.br/handle/11600/53576WOS000405469000009.pdf10.1016/j.scr.2017.05.013WOS:000405469000009The subventricular zone (SVZ) of the adult mammalian brain hosts full potential neural stem cells (NSCs). NSCs are able to respond to extracellular signals in the brain, amplifying the pool of progenitor cells and giving rise to neuroblasts that showability to migrate towards an injury site. These signals can come fromvascular system, cerebrospinal fluid, glial cells, or projections of neurons in adjoining regions. CXCL12, a chemokine secreted after brain injury, reaches the SVZ in a gradient manner and drives neuroblasts towards the lesion area. Among many other molecules, matrix metalloproteinase 2 and 9 (MMP-2/9) are also released during brain injury. MMP-2/9 can cleave CXCL12 generating a new molecule, CXCL12(5-67), and its effects on NSCs viability is not well described. Here we produced recombinant CXCL12 and CXCL12(5-67) and evaluated their effect inmurine adultNSCsmigration and survival in vitro. We showed CXCL12(5-67) does not promote NSCsmigration, but does induce cell death. The NSC death induced by CXCL12(5-67) involves caspases 9 and 3/7 activation, implying the intrinsic apoptotic pathway in this phenomenon. Our evidences in vitromake CXCL12(5-67) and its receptor potential candidates for brain injuries and neurodegeneration studies. (C) 2017 The Author(s). Published by Elsevier B.V.FAPESPCNPqUniv Fed Sao Paulo, Escola Paulista Med, Dept Biochem, BR-04039 Sao Paulo, BrazilUniv Fed Sao Paulo, Escola Paulista Med, Dept Biophys, BR-04044 Sao Paulo, BrazilUniv Fed Sao Paulo, Dept Cell Biol, BR-09920 Diadema, BrazilUniv Fed Sao Paulo, Escola Paulista Med, Dept Biochem, BR-04039 Sao Paulo, BrazilUniv Fed Sao Paulo, Escola Paulista Med, Dept Biophys, BR-04044 Sao Paulo, BrazilUniv Fed Sao Paulo, Dept Cell Biol, BR-09920 Diadema, BrazilFAPESP: 2011/11388-4FAPESP: 2012/00652-5FAPESP: 2015/19231-8CNPq: 404646/2012-3Web of Science61-69engElsevier Science BvStem Cell ResearchCXCL12CXCL12(5-67)Adult neural stem cellMigrationApoptosisIn vitro studyProteolytic processed form of CXCL12 abolishes migration and induces apoptosis in neural stem cells in vitroinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleAmsterdam22info:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000405469000009.pdfapplication/pdf1172711${dspace.ui.url}/bitstream/11600/53576/1/WOS000405469000009.pdfed268737c97aa27c51b61c3b73f613edMD51open accessTEXTWOS000405469000009.pdf.txtWOS000405469000009.pdf.txtExtracted texttext/plain50449${dspace.ui.url}/bitstream/11600/53576/8/WOS000405469000009.pdf.txta8b4a3151c572ea5bb9503847dbaf929MD58open accessTHUMBNAILWOS000405469000009.pdf.jpgWOS000405469000009.pdf.jpgIM Thumbnailimage/jpeg8290${dspace.ui.url}/bitstream/11600/53576/10/WOS000405469000009.pdf.jpgf1571345d9c67ded65a74d489119f84fMD510open access11600/535762023-06-05 19:28:06.625open accessoai:repositorio.unifesp.br:11600/53576Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-06-05T22:28:06Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Proteolytic processed form of CXCL12 abolishes migration and induces apoptosis in neural stem cells in vitro |
title |
Proteolytic processed form of CXCL12 abolishes migration and induces apoptosis in neural stem cells in vitro |
spellingShingle |
Proteolytic processed form of CXCL12 abolishes migration and induces apoptosis in neural stem cells in vitro Adelita, Tais [UNIFESP] CXCL12 CXCL12(5-67) Adult neural stem cell Migration Apoptosis In vitro study |
title_short |
Proteolytic processed form of CXCL12 abolishes migration and induces apoptosis in neural stem cells in vitro |
title_full |
Proteolytic processed form of CXCL12 abolishes migration and induces apoptosis in neural stem cells in vitro |
title_fullStr |
Proteolytic processed form of CXCL12 abolishes migration and induces apoptosis in neural stem cells in vitro |
title_full_unstemmed |
Proteolytic processed form of CXCL12 abolishes migration and induces apoptosis in neural stem cells in vitro |
title_sort |
Proteolytic processed form of CXCL12 abolishes migration and induces apoptosis in neural stem cells in vitro |
author |
Adelita, Tais [UNIFESP] |
author_facet |
Adelita, Tais [UNIFESP] Stilhano, Roberta Sessa [UNIFESP] Han, SangWon [UNIFESP] Justo, Giselle Zenker [UNIFESP] Porcionatto, Marimelia [UNIFESP] |
author_role |
author |
author2 |
Stilhano, Roberta Sessa [UNIFESP] Han, SangWon [UNIFESP] Justo, Giselle Zenker [UNIFESP] Porcionatto, Marimelia [UNIFESP] |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Adelita, Tais [UNIFESP] Stilhano, Roberta Sessa [UNIFESP] Han, SangWon [UNIFESP] Justo, Giselle Zenker [UNIFESP] Porcionatto, Marimelia [UNIFESP] |
dc.subject.eng.fl_str_mv |
CXCL12 CXCL12(5-67) Adult neural stem cell Migration Apoptosis In vitro study |
topic |
CXCL12 CXCL12(5-67) Adult neural stem cell Migration Apoptosis In vitro study |
description |
The subventricular zone (SVZ) of the adult mammalian brain hosts full potential neural stem cells (NSCs). NSCs are able to respond to extracellular signals in the brain, amplifying the pool of progenitor cells and giving rise to neuroblasts that showability to migrate towards an injury site. These signals can come fromvascular system, cerebrospinal fluid, glial cells, or projections of neurons in adjoining regions. CXCL12, a chemokine secreted after brain injury, reaches the SVZ in a gradient manner and drives neuroblasts towards the lesion area. Among many other molecules, matrix metalloproteinase 2 and 9 (MMP-2/9) are also released during brain injury. MMP-2/9 can cleave CXCL12 generating a new molecule, CXCL12(5-67), and its effects on NSCs viability is not well described. Here we produced recombinant CXCL12 and CXCL12(5-67) and evaluated their effect inmurine adultNSCsmigration and survival in vitro. We showed CXCL12(5-67) does not promote NSCsmigration, but does induce cell death. The NSC death induced by CXCL12(5-67) involves caspases 9 and 3/7 activation, implying the intrinsic apoptotic pathway in this phenomenon. Our evidences in vitromake CXCL12(5-67) and its receptor potential candidates for brain injuries and neurodegeneration studies. (C) 2017 The Author(s). Published by Elsevier B.V. |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017 |
dc.date.accessioned.fl_str_mv |
2020-06-26T16:30:30Z |
dc.date.available.fl_str_mv |
2020-06-26T16:30:30Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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article |
status_str |
publishedVersion |
dc.identifier.].fl_str_mv |
http://dx.doi.org/10.1016/j.scr.2017.05.013 |
dc.identifier.citation.fl_str_mv |
Stem Cell Research. Amsterdam, v. 22, p. 61-69, 2017. |
dc.identifier.uri.fl_str_mv |
https://repositorio.unifesp.br/handle/11600/53576 |
dc.identifier.issn.none.fl_str_mv |
1873-5061 |
dc.identifier.file.none.fl_str_mv |
WOS000405469000009.pdf |
dc.identifier.doi.none.fl_str_mv |
10.1016/j.scr.2017.05.013 |
dc.identifier.wos.none.fl_str_mv |
WOS:000405469000009 |
url |
http://dx.doi.org/10.1016/j.scr.2017.05.013 https://repositorio.unifesp.br/handle/11600/53576 |
identifier_str_mv |
Stem Cell Research. Amsterdam, v. 22, p. 61-69, 2017. 1873-5061 WOS000405469000009.pdf 10.1016/j.scr.2017.05.013 WOS:000405469000009 |
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Stem Cell Research |
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Elsevier Science Bv |
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Elsevier Science Bv |
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