Clinical implications of autoantibodies in HIV infection

Detalhes bibliográficos
Autor(a) principal: Massaki, Paulo Sergio [UNIFESP]
Data de Publicação: 1997
Outros Autores: Accetturi, Conceicao Aparecida [UNIFESP], Nishie, I. A., Silva, Neusa Pereira da [UNIFESP], Sato, Emília Inoue [UNIFESP], Andrade, Luís E. C [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1097/00002030-199715000-00009
http://repositorio.unifesp.br/handle/11600/25816
Resumo: Objective: To study the frequency and specificity of autoantibodies in HIV-infected subjects and their association with rheumatic manifestations, immunodeficiency, and prognosis.Design: Prospective study of sequentially selected HIV-infected patients. Indirect immunofluorescence reading was performed by two independent observers blinded for the patient diagnosis. Enzyme-linked immunosorbent assay (ELISA) was performed using coded serum samples.Setting: the study was performed at the Infectious Disease and Rheumatology Divisions of a tertiary care university hospital.Patients: One hundred sequentially selected HIV-infected patients formed group A. Controls included 80 non-HIV-infected high-risk individuals (group B), 20 herpesvirus-infected patients (group C), and 30 healthy blood donors (group D).Main outcome measures: Patients were followed for 2 years and evaluated for the presence of immunodeficiency, rheumatic manifestations, circulating autoantibodies and total CD4+ cell count. Indirect immunofluorescence was used to investigate antinuclear antibodies, antibodies to native DNA, smooth muscle, parietal cell, glomeruli, thyroid, and neutrophil cytoplasm. Agglutination was used to detect antibodies to erythrocytes and rheumatoid factor. ELISA was used to determine antibodies to cardiolipin and denatured DNA. CD4+ lymphocytes were counted by flow cytometry. Immunoglobulin (Ig) G, IgM and IgA serum levels were determined by radial immunodiffusion.Results: HIV-infected patients presented higher overall frequency of autoantibodies than the other groups. No difference was observed between immunodeficient and asymptomatic HIV-infected patients. the most frequent specificities were antibodies to cardiolipin and to denatured DNA. Ig serum levels did not correlate with the occurrence of autoantibodies. the presence of autoantibodies was associated with lower CD4+ cell counts and with higher mortality within 2 years. Rheumatic manifestations were observed in 35 HIV-infected patients and were not associated with the occurrence of autoantibodies or the presence of immunodeficiency.Conclusions: HIV infection is associated with an increased incidence of autoantibodies. Although not related to the occurrence of rheumatic manifestations, the presence of autoantibodies was significantly associated with lower CD4+ lymphocyte counts and increased mortality, which implies prognostic significance to this phenomenon in the context of HIV infection.
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spelling Clinical implications of autoantibodies in HIV infectionAIDSautoantibodiesantinuclear antibodiesObjective: To study the frequency and specificity of autoantibodies in HIV-infected subjects and their association with rheumatic manifestations, immunodeficiency, and prognosis.Design: Prospective study of sequentially selected HIV-infected patients. Indirect immunofluorescence reading was performed by two independent observers blinded for the patient diagnosis. Enzyme-linked immunosorbent assay (ELISA) was performed using coded serum samples.Setting: the study was performed at the Infectious Disease and Rheumatology Divisions of a tertiary care university hospital.Patients: One hundred sequentially selected HIV-infected patients formed group A. Controls included 80 non-HIV-infected high-risk individuals (group B), 20 herpesvirus-infected patients (group C), and 30 healthy blood donors (group D).Main outcome measures: Patients were followed for 2 years and evaluated for the presence of immunodeficiency, rheumatic manifestations, circulating autoantibodies and total CD4+ cell count. Indirect immunofluorescence was used to investigate antinuclear antibodies, antibodies to native DNA, smooth muscle, parietal cell, glomeruli, thyroid, and neutrophil cytoplasm. Agglutination was used to detect antibodies to erythrocytes and rheumatoid factor. ELISA was used to determine antibodies to cardiolipin and denatured DNA. CD4+ lymphocytes were counted by flow cytometry. Immunoglobulin (Ig) G, IgM and IgA serum levels were determined by radial immunodiffusion.Results: HIV-infected patients presented higher overall frequency of autoantibodies than the other groups. No difference was observed between immunodeficient and asymptomatic HIV-infected patients. the most frequent specificities were antibodies to cardiolipin and to denatured DNA. Ig serum levels did not correlate with the occurrence of autoantibodies. the presence of autoantibodies was associated with lower CD4+ cell counts and with higher mortality within 2 years. Rheumatic manifestations were observed in 35 HIV-infected patients and were not associated with the occurrence of autoantibodies or the presence of immunodeficiency.Conclusions: HIV infection is associated with an increased incidence of autoantibodies. Although not related to the occurrence of rheumatic manifestations, the presence of autoantibodies was significantly associated with lower CD4+ lymphocyte counts and increased mortality, which implies prognostic significance to this phenomenon in the context of HIV infection.Universidade Federal de São Paulo, DIV RHEUMATOL, ESCOLA PAULISTA MED, BR-04023900 São Paulo, BRAZILUniversidade Federal de São Paulo, DIV INFECT DIS, ESCOLA PAULISTA MED, BR-04023900 São Paulo, BRAZILUniversidade Federal de São Paulo, DIV RHEUMATOL, ESCOLA PAULISTA MED, BR-04023900 São Paulo, BRAZILUniversidade Federal de São Paulo, DIV INFECT DIS, ESCOLA PAULISTA MED, BR-04023900 São Paulo, BRAZILWeb of ScienceLippincott Williams & WilkinsUniversidade Federal de São Paulo (UNIFESP)Massaki, Paulo Sergio [UNIFESP]Accetturi, Conceicao Aparecida [UNIFESP]Nishie, I. A.Silva, Neusa Pereira da [UNIFESP]Sato, Emília Inoue [UNIFESP]Andrade, Luís E. C [UNIFESP]2016-01-24T12:30:29Z2016-01-24T12:30:29Z1997-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion1845-1850http://dx.doi.org/10.1097/00002030-199715000-00009Aids. Philadelphia: Lippincott Williams & Wilkins, v. 11, n. 15, p. 1845-1850, 1997.10.1097/00002030-199715000-000090269-9370http://repositorio.unifesp.br/handle/11600/25816WOS:A1997YH35800009engAidsinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2023-01-30T22:20:24Zoai:repositorio.unifesp.br/:11600/25816Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652023-01-30T22:20:24Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Clinical implications of autoantibodies in HIV infection
title Clinical implications of autoantibodies in HIV infection
spellingShingle Clinical implications of autoantibodies in HIV infection
Massaki, Paulo Sergio [UNIFESP]
AIDS
autoantibodies
antinuclear antibodies
title_short Clinical implications of autoantibodies in HIV infection
title_full Clinical implications of autoantibodies in HIV infection
title_fullStr Clinical implications of autoantibodies in HIV infection
title_full_unstemmed Clinical implications of autoantibodies in HIV infection
title_sort Clinical implications of autoantibodies in HIV infection
author Massaki, Paulo Sergio [UNIFESP]
author_facet Massaki, Paulo Sergio [UNIFESP]
Accetturi, Conceicao Aparecida [UNIFESP]
Nishie, I. A.
Silva, Neusa Pereira da [UNIFESP]
Sato, Emília Inoue [UNIFESP]
Andrade, Luís E. C [UNIFESP]
author_role author
author2 Accetturi, Conceicao Aparecida [UNIFESP]
Nishie, I. A.
Silva, Neusa Pereira da [UNIFESP]
Sato, Emília Inoue [UNIFESP]
Andrade, Luís E. C [UNIFESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Massaki, Paulo Sergio [UNIFESP]
Accetturi, Conceicao Aparecida [UNIFESP]
Nishie, I. A.
Silva, Neusa Pereira da [UNIFESP]
Sato, Emília Inoue [UNIFESP]
Andrade, Luís E. C [UNIFESP]
dc.subject.por.fl_str_mv AIDS
autoantibodies
antinuclear antibodies
topic AIDS
autoantibodies
antinuclear antibodies
description Objective: To study the frequency and specificity of autoantibodies in HIV-infected subjects and their association with rheumatic manifestations, immunodeficiency, and prognosis.Design: Prospective study of sequentially selected HIV-infected patients. Indirect immunofluorescence reading was performed by two independent observers blinded for the patient diagnosis. Enzyme-linked immunosorbent assay (ELISA) was performed using coded serum samples.Setting: the study was performed at the Infectious Disease and Rheumatology Divisions of a tertiary care university hospital.Patients: One hundred sequentially selected HIV-infected patients formed group A. Controls included 80 non-HIV-infected high-risk individuals (group B), 20 herpesvirus-infected patients (group C), and 30 healthy blood donors (group D).Main outcome measures: Patients were followed for 2 years and evaluated for the presence of immunodeficiency, rheumatic manifestations, circulating autoantibodies and total CD4+ cell count. Indirect immunofluorescence was used to investigate antinuclear antibodies, antibodies to native DNA, smooth muscle, parietal cell, glomeruli, thyroid, and neutrophil cytoplasm. Agglutination was used to detect antibodies to erythrocytes and rheumatoid factor. ELISA was used to determine antibodies to cardiolipin and denatured DNA. CD4+ lymphocytes were counted by flow cytometry. Immunoglobulin (Ig) G, IgM and IgA serum levels were determined by radial immunodiffusion.Results: HIV-infected patients presented higher overall frequency of autoantibodies than the other groups. No difference was observed between immunodeficient and asymptomatic HIV-infected patients. the most frequent specificities were antibodies to cardiolipin and to denatured DNA. Ig serum levels did not correlate with the occurrence of autoantibodies. the presence of autoantibodies was associated with lower CD4+ cell counts and with higher mortality within 2 years. Rheumatic manifestations were observed in 35 HIV-infected patients and were not associated with the occurrence of autoantibodies or the presence of immunodeficiency.Conclusions: HIV infection is associated with an increased incidence of autoantibodies. Although not related to the occurrence of rheumatic manifestations, the presence of autoantibodies was significantly associated with lower CD4+ lymphocyte counts and increased mortality, which implies prognostic significance to this phenomenon in the context of HIV infection.
publishDate 1997
dc.date.none.fl_str_mv 1997-12-01
2016-01-24T12:30:29Z
2016-01-24T12:30:29Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1097/00002030-199715000-00009
Aids. Philadelphia: Lippincott Williams & Wilkins, v. 11, n. 15, p. 1845-1850, 1997.
10.1097/00002030-199715000-00009
0269-9370
http://repositorio.unifesp.br/handle/11600/25816
WOS:A1997YH35800009
url http://dx.doi.org/10.1097/00002030-199715000-00009
http://repositorio.unifesp.br/handle/11600/25816
identifier_str_mv Aids. Philadelphia: Lippincott Williams & Wilkins, v. 11, n. 15, p. 1845-1850, 1997.
10.1097/00002030-199715000-00009
0269-9370
WOS:A1997YH35800009
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Aids
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1845-1850
dc.publisher.none.fl_str_mv Lippincott Williams & Wilkins
publisher.none.fl_str_mv Lippincott Williams & Wilkins
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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