Sistema RANK/RANKL e OPG em cultura de fibroblastos adultos do ligamento periodontal humano sob pressão contínua

Detalhes bibliográficos
Autor(a) principal: Pereira, Mateus de Abreu [UNIFESP]
Data de Publicação: 2016
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: https://sucupira.capes.gov.br/sucupira/public/consultas/coleta/trabalhoConclusao/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=3862286
http://repositorio.unifesp.br/handle/11600/47185
Resumo: Introduction: The cultive of periodontal ligament fibroblasts associated with simulation of pressure or tension on the cell allows analysis in its morphology and form, identify proteins and molecules involved in this process. The system formed by the protein Nuclear Factor Activator Receptor ?B (RANK) \ Nuclear factor Activator Receptor ?B Ligand (RANKL) and osteoprotegerin (OPG) are directly linked to the regulation of osteoclast and osteoclastogenesis, formation and / or bone resorption. Objective: Evaluate the fibroblasts of RANK \ RANKL and OPG in adult fibroblasts of human periodontal ligament under continuous pressure. Methods: The tissue was collected from periodontal ligament of 40 third molars of adult patients who were grown in culture flasks until the sixth passage. The cells were placed on plates with 6 wells with a 3 mm diameter which were subjected to the continuous pressure (3 to 4 g / mm2) for 6 hours in monolayers. From the samples, the mRNA was extracted and evaluated by method Polymerase Chain Reaction Real-Time (qPCR).Results: In the continuous pressure model by using the fibroblasts of the periodontal ligament, in all the groups there was no expression of RANK \ RANKL, and all groups expressed OPG, and was significantly higher in the experimental group 2 (EG2) compared to control (CG) and experimental group 1 (EG1) (P = 0.005). Conclusion: The adult fibroblast from human periodontal ligament when under continuous pressure not expressed mRNA RANK \ RANKL and osteoprotegerin mRNA was expressed.
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spelling Sistema RANK/RANKL e OPG em cultura de fibroblastos adultos do ligamento periodontal humano sob pressão contínuaSystem RANK/RANKL and OPG in cultured adult human periodontal ligament fibroblast under pressure continuesLigamento periodontalLigante rankOsteoprotegerinaFibroblastosIntroduction: The cultive of periodontal ligament fibroblasts associated with simulation of pressure or tension on the cell allows analysis in its morphology and form, identify proteins and molecules involved in this process. The system formed by the protein Nuclear Factor Activator Receptor ?B (RANK) \ Nuclear factor Activator Receptor ?B Ligand (RANKL) and osteoprotegerin (OPG) are directly linked to the regulation of osteoclast and osteoclastogenesis, formation and / or bone resorption. Objective: Evaluate the fibroblasts of RANK \ RANKL and OPG in adult fibroblasts of human periodontal ligament under continuous pressure. Methods: The tissue was collected from periodontal ligament of 40 third molars of adult patients who were grown in culture flasks until the sixth passage. The cells were placed on plates with 6 wells with a 3 mm diameter which were subjected to the continuous pressure (3 to 4 g / mm2) for 6 hours in monolayers. From the samples, the mRNA was extracted and evaluated by method Polymerase Chain Reaction Real-Time (qPCR).Results: In the continuous pressure model by using the fibroblasts of the periodontal ligament, in all the groups there was no expression of RANK \ RANKL, and all groups expressed OPG, and was significantly higher in the experimental group 2 (EG2) compared to control (CG) and experimental group 1 (EG1) (P = 0.005). Conclusion: The adult fibroblast from human periodontal ligament when under continuous pressure not expressed mRNA RANK \ RANKL and osteoprotegerin mRNA was expressed.Introdução: O cultivo de fibroblastos do ligamento periodontal, associada a simulação de cargas de pressão ou tensão sobre a célula, permite analisar as modificações da sua morfologia e identificar proteínas e moléculas formadas e envolvidas neste processo. O sistema formado pelas proteínas receptor ativador de fator nuclear ?B (RANK), receptor ativador de fator nuclear ?B Ligante (RANKL) e a osteoprotegerina (OPG) está diretamente ligado a regulação de osteoclastos e a osteoclastogênese, na formação e reabsorção do tecido ósseo. Objetivo: Avaliar a expressão do RNAm das proteínas RANK\RANKL e OPG em fibroblastos adultos do ligamento periodontal humano sob pressão contínua. Métodos: Os fibroblastos foram coletados do ligamento periodontal de 40 terceiros molares inclusos de pacientes adultos e foram cultivados em frascos de culturas até a sexta passagem. As células foram alojadas em placas com 6 poços com 3 mm de diâmetro onde foram submetidos a pressão contínua (3 e 4 g/cm2 ) durante 6 horas em monocamadas. Das amostras, o RNAm foi extraído e avaliado pelo método de Reação em Cadeia de Polimerase em Tempo Real qPCR Resultados: No modelo de pressão contínua utilizando fibroblastos do ligamento periodontal, nos grupos avaliados, não houve a expressão de RANK\RANKL, mas expressaram OPG, tendo sido significativamente maior no grupo experimental 2 (GE2) em comparação com os grupos controle (GC) e experimental 1 (GE1). Conclusão: Os fibroblastos adultos de ligamento periodontal humano quando sob pressão contínua não expressaram o RNAm de RANK\RANKL e expressaram o RNAm da Osteoprotegerina.Dados abertos - Sucupira - Teses e dissertações (2013 a 2016)Universidade Federal de São Paulo (UNIFESP)Ferreira, Lydia Masako [UNIFESP]http://lattes.cnpq.br/1619822351741819http://lattes.cnpq.br/1607299661585252Universidade Federal de São Paulo (UNIFESP)Pereira, Mateus de Abreu [UNIFESP]2018-07-30T11:43:54Z2018-07-30T11:43:54Z2016-05-20info:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/publishedVersion77 F.application/pdfhttps://sucupira.capes.gov.br/sucupira/public/consultas/coleta/trabalhoConclusao/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=3862286PEREIRA, Mateus de Abreu. Sistema RANK/RANKL e OPG em cultura de fibroblastos adultos do ligamento periodontal humano sob pressão contínua. 2016. 77 f. Dissertação (Mestrado em Cirurgia Translacional) - Escola Paulista de Medicina, Universidade Federal de São Paulo (UNIFESP), São Paulo, 2016.MATEUS DE ABREU PEREIRA - PDF A.pdfhttp://repositorio.unifesp.br/handle/11600/47185porSão Pauloinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-01T13:47:09Zoai:repositorio.unifesp.br/:11600/47185Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-01T13:47:09Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Sistema RANK/RANKL e OPG em cultura de fibroblastos adultos do ligamento periodontal humano sob pressão contínua
System RANK/RANKL and OPG in cultured adult human periodontal ligament fibroblast under pressure continues
title Sistema RANK/RANKL e OPG em cultura de fibroblastos adultos do ligamento periodontal humano sob pressão contínua
spellingShingle Sistema RANK/RANKL e OPG em cultura de fibroblastos adultos do ligamento periodontal humano sob pressão contínua
Pereira, Mateus de Abreu [UNIFESP]
Ligamento periodontal
Ligante rank
Osteoprotegerina
Fibroblastos
title_short Sistema RANK/RANKL e OPG em cultura de fibroblastos adultos do ligamento periodontal humano sob pressão contínua
title_full Sistema RANK/RANKL e OPG em cultura de fibroblastos adultos do ligamento periodontal humano sob pressão contínua
title_fullStr Sistema RANK/RANKL e OPG em cultura de fibroblastos adultos do ligamento periodontal humano sob pressão contínua
title_full_unstemmed Sistema RANK/RANKL e OPG em cultura de fibroblastos adultos do ligamento periodontal humano sob pressão contínua
title_sort Sistema RANK/RANKL e OPG em cultura de fibroblastos adultos do ligamento periodontal humano sob pressão contínua
author Pereira, Mateus de Abreu [UNIFESP]
author_facet Pereira, Mateus de Abreu [UNIFESP]
author_role author
dc.contributor.none.fl_str_mv Ferreira, Lydia Masako [UNIFESP]
http://lattes.cnpq.br/1619822351741819
http://lattes.cnpq.br/1607299661585252
Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Pereira, Mateus de Abreu [UNIFESP]
dc.subject.por.fl_str_mv Ligamento periodontal
Ligante rank
Osteoprotegerina
Fibroblastos
topic Ligamento periodontal
Ligante rank
Osteoprotegerina
Fibroblastos
description Introduction: The cultive of periodontal ligament fibroblasts associated with simulation of pressure or tension on the cell allows analysis in its morphology and form, identify proteins and molecules involved in this process. The system formed by the protein Nuclear Factor Activator Receptor ?B (RANK) \ Nuclear factor Activator Receptor ?B Ligand (RANKL) and osteoprotegerin (OPG) are directly linked to the regulation of osteoclast and osteoclastogenesis, formation and / or bone resorption. Objective: Evaluate the fibroblasts of RANK \ RANKL and OPG in adult fibroblasts of human periodontal ligament under continuous pressure. Methods: The tissue was collected from periodontal ligament of 40 third molars of adult patients who were grown in culture flasks until the sixth passage. The cells were placed on plates with 6 wells with a 3 mm diameter which were subjected to the continuous pressure (3 to 4 g / mm2) for 6 hours in monolayers. From the samples, the mRNA was extracted and evaluated by method Polymerase Chain Reaction Real-Time (qPCR).Results: In the continuous pressure model by using the fibroblasts of the periodontal ligament, in all the groups there was no expression of RANK \ RANKL, and all groups expressed OPG, and was significantly higher in the experimental group 2 (EG2) compared to control (CG) and experimental group 1 (EG1) (P = 0.005). Conclusion: The adult fibroblast from human periodontal ligament when under continuous pressure not expressed mRNA RANK \ RANKL and osteoprotegerin mRNA was expressed.
publishDate 2016
dc.date.none.fl_str_mv 2016-05-20
2018-07-30T11:43:54Z
2018-07-30T11:43:54Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://sucupira.capes.gov.br/sucupira/public/consultas/coleta/trabalhoConclusao/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=3862286
PEREIRA, Mateus de Abreu. Sistema RANK/RANKL e OPG em cultura de fibroblastos adultos do ligamento periodontal humano sob pressão contínua. 2016. 77 f. Dissertação (Mestrado em Cirurgia Translacional) - Escola Paulista de Medicina, Universidade Federal de São Paulo (UNIFESP), São Paulo, 2016.
MATEUS DE ABREU PEREIRA - PDF A.pdf
http://repositorio.unifesp.br/handle/11600/47185
url https://sucupira.capes.gov.br/sucupira/public/consultas/coleta/trabalhoConclusao/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=3862286
http://repositorio.unifesp.br/handle/11600/47185
identifier_str_mv PEREIRA, Mateus de Abreu. Sistema RANK/RANKL e OPG em cultura de fibroblastos adultos do ligamento periodontal humano sob pressão contínua. 2016. 77 f. Dissertação (Mestrado em Cirurgia Translacional) - Escola Paulista de Medicina, Universidade Federal de São Paulo (UNIFESP), São Paulo, 2016.
MATEUS DE ABREU PEREIRA - PDF A.pdf
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 77 F.
application/pdf
dc.coverage.none.fl_str_mv São Paulo
dc.publisher.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
publisher.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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