A Carbohydrate Moiety of Secreted Stage-Specific Glycoprotein 4 Participates in Host Cell Invasion by Trypanosoma cruzi Extracellular Amastigotes
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://dx.doi.org/10.3389/fmicb.2018.00693 https://repositorio.unifesp.br/handle/11600/55639 |
Resumo: | Trypanosoma cruzi is the etiologic agent of Chagas' disease. It is known that amastigotes derived from trypomastigotes in the extracellular milieu are infective in vitro and in vivo. Extracellular amastigotes (EAs) have a stage-specific surface antigen called Ssp-4, a GPI-anchored glycoprotein that is secreted by the parasites. By immunoprecipitation with the Ssp-4-specific monoclonal antibodies (mAb) 2C2 and 1D9, we isolated the glycoprotein from EAs. By mass spectrometry, we identified the core protein of Ssp-4 and evaluated mRNA expression and the presence of Ssp-4 carbohydrate epitopes recognized by mAb1D9. We demonstrated that the carbohydrate epitope recognized by mAb1D9 could promote host cell invasion by EAs. Although infectious EAs express lower amounts of Ssp-4 compared with less-infectious EAs (at the mRNA and protein levels), it is the glycosylation of Ssp-4 (identified by mAb1D9 staining only in infectious strains and recognized by galectin-3 on host cells) that is the determinant of EA invasion of host cells. Furthermore, Ssp-4 is secreted by EAs, either free or associated with parasite vesicles, and can participate in host-cell interactions. The results presented here describe the possible role of a carbohydrate moiety of T. cruzi surface glycoproteins in host cell invasion by EA forms, highlighting the potential of these moieties as therapeutic and vaccine targets for the treatment of Chagas' disease. |
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A Carbohydrate Moiety of Secreted Stage-Specific Glycoprotein 4 Participates in Host Cell Invasion by Trypanosoma cruzi Extracellular AmastigotesTrypanosoma cruziextracellular amastigotesSsp-4 glycoproteingalectin-3Trypanosoma cruzi is the etiologic agent of Chagas' disease. It is known that amastigotes derived from trypomastigotes in the extracellular milieu are infective in vitro and in vivo. Extracellular amastigotes (EAs) have a stage-specific surface antigen called Ssp-4, a GPI-anchored glycoprotein that is secreted by the parasites. By immunoprecipitation with the Ssp-4-specific monoclonal antibodies (mAb) 2C2 and 1D9, we isolated the glycoprotein from EAs. By mass spectrometry, we identified the core protein of Ssp-4 and evaluated mRNA expression and the presence of Ssp-4 carbohydrate epitopes recognized by mAb1D9. We demonstrated that the carbohydrate epitope recognized by mAb1D9 could promote host cell invasion by EAs. Although infectious EAs express lower amounts of Ssp-4 compared with less-infectious EAs (at the mRNA and protein levels), it is the glycosylation of Ssp-4 (identified by mAb1D9 staining only in infectious strains and recognized by galectin-3 on host cells) that is the determinant of EA invasion of host cells. Furthermore, Ssp-4 is secreted by EAs, either free or associated with parasite vesicles, and can participate in host-cell interactions. The results presented here describe the possible role of a carbohydrate moiety of T. cruzi surface glycoproteins in host cell invasion by EA forms, highlighting the potential of these moieties as therapeutic and vaccine targets for the treatment of Chagas' disease.Univ Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, Sao Paulo, BrazilINSERM, Inst Cochin, U1016, Paris, FranceUniv Fed Sao Paulo, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, Sao Paulo, BrazilButantan Inst, Immune Response & Cell Signaling CeTICS, Ctr Toxins, Special Lab Cell Cycle, Sao Paulo, BrazilUniv Mayor, Ctr Genom & Bioinformat, Fac Ciencias, Santiago, ChilePurdue Univ, Bindley Biosci Ctr, W Lafayette, IN USAUniv Fed Sao Paulo, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, Sao Paulo, BrazilWeb of ScienceFundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)FAPESP: 2011/51475-3FAPESP: 2012/23150-5CNPq: 302068/2016-3Frontiers Media Sa2020-07-20T16:31:00Z2020-07-20T16:31:00Z2018info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion-application/pdfhttp://dx.doi.org/10.3389/fmicb.2018.00693Frontiers In Microbiology. Lausanne, v. 9, p. -, 2018.10.3389/fmicb.2018.00693WOS000429562900001.pdf1664-302Xhttps://repositorio.unifesp.br/handle/11600/55639WOS:000429562900001engFrontiers In MicrobiologyLausanneinfo:eu-repo/semantics/openAccessFlorentino, Pilar T. V.Real, FernandoOrikaza, Cristina Mary [UNIFESP]Cunha, Julia P. C. daVitorino, Francisca N. L.Cordero, Esteban M.Sobreira, Tiago J. P.Mortara, Renato Arruda [UNIFESP]reponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-03T08:05:50Zoai:repositorio.unifesp.br/:11600/55639Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-03T08:05:50Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
A Carbohydrate Moiety of Secreted Stage-Specific Glycoprotein 4 Participates in Host Cell Invasion by Trypanosoma cruzi Extracellular Amastigotes |
title |
A Carbohydrate Moiety of Secreted Stage-Specific Glycoprotein 4 Participates in Host Cell Invasion by Trypanosoma cruzi Extracellular Amastigotes |
spellingShingle |
A Carbohydrate Moiety of Secreted Stage-Specific Glycoprotein 4 Participates in Host Cell Invasion by Trypanosoma cruzi Extracellular Amastigotes Florentino, Pilar T. V. Trypanosoma cruzi extracellular amastigotes Ssp-4 glycoprotein galectin-3 |
title_short |
A Carbohydrate Moiety of Secreted Stage-Specific Glycoprotein 4 Participates in Host Cell Invasion by Trypanosoma cruzi Extracellular Amastigotes |
title_full |
A Carbohydrate Moiety of Secreted Stage-Specific Glycoprotein 4 Participates in Host Cell Invasion by Trypanosoma cruzi Extracellular Amastigotes |
title_fullStr |
A Carbohydrate Moiety of Secreted Stage-Specific Glycoprotein 4 Participates in Host Cell Invasion by Trypanosoma cruzi Extracellular Amastigotes |
title_full_unstemmed |
A Carbohydrate Moiety of Secreted Stage-Specific Glycoprotein 4 Participates in Host Cell Invasion by Trypanosoma cruzi Extracellular Amastigotes |
title_sort |
A Carbohydrate Moiety of Secreted Stage-Specific Glycoprotein 4 Participates in Host Cell Invasion by Trypanosoma cruzi Extracellular Amastigotes |
author |
Florentino, Pilar T. V. |
author_facet |
Florentino, Pilar T. V. Real, Fernando Orikaza, Cristina Mary [UNIFESP] Cunha, Julia P. C. da Vitorino, Francisca N. L. Cordero, Esteban M. Sobreira, Tiago J. P. Mortara, Renato Arruda [UNIFESP] |
author_role |
author |
author2 |
Real, Fernando Orikaza, Cristina Mary [UNIFESP] Cunha, Julia P. C. da Vitorino, Francisca N. L. Cordero, Esteban M. Sobreira, Tiago J. P. Mortara, Renato Arruda [UNIFESP] |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Florentino, Pilar T. V. Real, Fernando Orikaza, Cristina Mary [UNIFESP] Cunha, Julia P. C. da Vitorino, Francisca N. L. Cordero, Esteban M. Sobreira, Tiago J. P. Mortara, Renato Arruda [UNIFESP] |
dc.subject.por.fl_str_mv |
Trypanosoma cruzi extracellular amastigotes Ssp-4 glycoprotein galectin-3 |
topic |
Trypanosoma cruzi extracellular amastigotes Ssp-4 glycoprotein galectin-3 |
description |
Trypanosoma cruzi is the etiologic agent of Chagas' disease. It is known that amastigotes derived from trypomastigotes in the extracellular milieu are infective in vitro and in vivo. Extracellular amastigotes (EAs) have a stage-specific surface antigen called Ssp-4, a GPI-anchored glycoprotein that is secreted by the parasites. By immunoprecipitation with the Ssp-4-specific monoclonal antibodies (mAb) 2C2 and 1D9, we isolated the glycoprotein from EAs. By mass spectrometry, we identified the core protein of Ssp-4 and evaluated mRNA expression and the presence of Ssp-4 carbohydrate epitopes recognized by mAb1D9. We demonstrated that the carbohydrate epitope recognized by mAb1D9 could promote host cell invasion by EAs. Although infectious EAs express lower amounts of Ssp-4 compared with less-infectious EAs (at the mRNA and protein levels), it is the glycosylation of Ssp-4 (identified by mAb1D9 staining only in infectious strains and recognized by galectin-3 on host cells) that is the determinant of EA invasion of host cells. Furthermore, Ssp-4 is secreted by EAs, either free or associated with parasite vesicles, and can participate in host-cell interactions. The results presented here describe the possible role of a carbohydrate moiety of T. cruzi surface glycoproteins in host cell invasion by EA forms, highlighting the potential of these moieties as therapeutic and vaccine targets for the treatment of Chagas' disease. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018 2020-07-20T16:31:00Z 2020-07-20T16:31:00Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3389/fmicb.2018.00693 Frontiers In Microbiology. Lausanne, v. 9, p. -, 2018. 10.3389/fmicb.2018.00693 WOS000429562900001.pdf 1664-302X https://repositorio.unifesp.br/handle/11600/55639 WOS:000429562900001 |
url |
http://dx.doi.org/10.3389/fmicb.2018.00693 https://repositorio.unifesp.br/handle/11600/55639 |
identifier_str_mv |
Frontiers In Microbiology. Lausanne, v. 9, p. -, 2018. 10.3389/fmicb.2018.00693 WOS000429562900001.pdf 1664-302X WOS:000429562900001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Frontiers In Microbiology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
- application/pdf |
dc.coverage.none.fl_str_mv |
Lausanne |
dc.publisher.none.fl_str_mv |
Frontiers Media Sa |
publisher.none.fl_str_mv |
Frontiers Media Sa |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
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1814268459302780928 |