Genetic Vaccination against Experimental Infection with Myotropic Parasite Strains of Trypanosoma cruzi
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | https://repositorio.unifesp.br/handle/11600/37146 https://dx.doi.org/10.1155/2014/605023 |
Resumo: | In earlier studies, we reported that a heterologous prime-boost regimen using recombinant plasmid DNA followed by replication-defective adenovirus vector, both containing Trypanosoma cruzi genes encoding trans-sialidase (TS) and amastigote surface protein (ASP) 2, provided protective immunity against experimental infection with a reticulotropic strain of this human protozoan parasite. Herein, we tested the outcome of genetic vaccination of F1 (CB10XBALB/c) mice challenged with myotropic parasite strains (Brazil and Colombian). Initially, we determined that the coadministration during priming of a DNA plasmid containing the murine IL-12 gene improved the immune response and was essential for protective immunity elicited by the heterologous prime-boost regimen in susceptible male mice against acute lethal infections with these parasites. the prophylactic or therapeutic vaccination of resistant female mice led to a drastic reduction in the number of inflammatory infiltrates in cardiac and skeletal muscles during the chronic phase of infection with either strain. Analysis of the electrocardiographic parameters showed that prophylactic vaccination reduced the frequencies of sinus arrhythmia and atrioventricular block. Our results confirmed that prophylactic vaccination using the TS and ASP-2 genes benefits the host against acute and chronic pathologies caused by T. cruzi and should be further evaluated for the development of a veterinary or human vaccine against Chagas disease. |
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Araujo, Adriano Fernando [UNIFESP]Oliveira, Gabriel deVasconcelos, Juliana FragaErsching, Jonatan [UNIFESP]Dominguez, Mariana Ribeiro [UNIFESP]Vasconcelos, Jose Ronnie Carvalho de [UNIFESP]Machado, Alexandre VieiraGazzinelli, Ricardo TostesBruna-Romero, OscarSoares, Milena BotelhoRodrigues, Mauricio Martins [UNIFESP]Universidade Federal de São Paulo (UNIFESP)Fiocruz MSHosp Sao RafaelUniversidade Federal de Minas Gerais (UFMG)Univ MassachusettsUniversidade Federal de Santa Catarina (UFSC)2016-01-24T14:34:56Z2016-01-24T14:34:56Z2014-01-01Mediators of Inflammation. New York: Hindawi Publishing Corporation, 13 p., 2014.0962-9351https://repositorio.unifesp.br/handle/11600/37146https://dx.doi.org/10.1155/2014/605023WOS000338549100001.pdf10.1155/2014/605023WOS:000338549100001In earlier studies, we reported that a heterologous prime-boost regimen using recombinant plasmid DNA followed by replication-defective adenovirus vector, both containing Trypanosoma cruzi genes encoding trans-sialidase (TS) and amastigote surface protein (ASP) 2, provided protective immunity against experimental infection with a reticulotropic strain of this human protozoan parasite. Herein, we tested the outcome of genetic vaccination of F1 (CB10XBALB/c) mice challenged with myotropic parasite strains (Brazil and Colombian). Initially, we determined that the coadministration during priming of a DNA plasmid containing the murine IL-12 gene improved the immune response and was essential for protective immunity elicited by the heterologous prime-boost regimen in susceptible male mice against acute lethal infections with these parasites. the prophylactic or therapeutic vaccination of resistant female mice led to a drastic reduction in the number of inflammatory infiltrates in cardiac and skeletal muscles during the chronic phase of infection with either strain. Analysis of the electrocardiographic parameters showed that prophylactic vaccination reduced the frequencies of sinus arrhythmia and atrioventricular block. Our results confirmed that prophylactic vaccination using the TS and ASP-2 genes benefits the host against acute and chronic pathologies caused by T. cruzi and should be further evaluated for the development of a veterinary or human vaccine against Chagas disease.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Universidade Federal de São Paulo, Escola Paulista Med, Ctr Terapia Celular & Mol CTCMol, BR-04044010 São Paulo, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, BR-04044010 São Paulo, BrazilFiocruz MS, Inst Oswaldo Cruz, Lab Biol Celular, BR-21040360 Rio de Janeiro, RJ, BrazilFiocruz MS, Ctr Pesquisas Goncalo Moniz, BR-40296710 Salvador, BA, BrazilHosp Sao Rafael, BR-41253190 Salvador, BA, BrazilUNIFESP, Inst Saude Soc, Dept Biociencias, BR-11015020 Santos, SP, BrazilFiocruz MS, Ctr Pesquisas Rene Rachou, BR-30190002 Belo Horizonte, MG, BrazilUniv Fed Minas Gerais, Inst Ciencias Biol, Dept Bioquim & Imunol, BR-31270901 Belo Horizonte, MG, BrazilUniv Massachusetts, Sch Med, Dept Med, Div Infect Dis & Immunol, Worcester, MA 01655 USAUniv Fed Santa Catarina, Dept Microbiol Immunol & Parasitol, BR-88040900 Florianopolis, SC, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Ctr Terapia Celular & Mol CTCMol, BR-04044010 São Paulo, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, BR-04044010 São Paulo, BrazilUNIFESP, Inst Saude Soc, Dept Biociencias, BR-11015020 Santos, SP, BrazilFAPESP: 2009/06820-4FAPESP: 2013/13668/0FAPESP: 2012/22514-3Web of Science13 f.engHindawi Publishing CorporationMediators of InflammationGenetic Vaccination against Experimental Infection with Myotropic Parasite Strains of Trypanosoma cruziinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000338549100001.pdfapplication/pdf1985687${dspace.ui.url}/bitstream/11600/37146/1/WOS000338549100001.pdf982fba8723c861b375d5cd50ca36ad44MD51open accessTEXTWOS000338549100001.pdf.txtWOS000338549100001.pdf.txtExtracted texttext/plain49558${dspace.ui.url}/bitstream/11600/37146/6/WOS000338549100001.pdf.txt82a44b24c5aba8cf37db1cf4d6ecbb4bMD56open accessTHUMBNAILWOS000338549100001.pdf.jpgWOS000338549100001.pdf.jpgIM Thumbnailimage/jpeg6475${dspace.ui.url}/bitstream/11600/37146/8/WOS000338549100001.pdf.jpg662b23b80eed3c8f1c6b058798f35316MD58open access11600/371462023-06-05 19:08:10.983open accessoai:repositorio.unifesp.br:11600/37146Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-06-05T22:08:10Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Genetic Vaccination against Experimental Infection with Myotropic Parasite Strains of Trypanosoma cruzi |
title |
Genetic Vaccination against Experimental Infection with Myotropic Parasite Strains of Trypanosoma cruzi |
spellingShingle |
Genetic Vaccination against Experimental Infection with Myotropic Parasite Strains of Trypanosoma cruzi Araujo, Adriano Fernando [UNIFESP] |
title_short |
Genetic Vaccination against Experimental Infection with Myotropic Parasite Strains of Trypanosoma cruzi |
title_full |
Genetic Vaccination against Experimental Infection with Myotropic Parasite Strains of Trypanosoma cruzi |
title_fullStr |
Genetic Vaccination against Experimental Infection with Myotropic Parasite Strains of Trypanosoma cruzi |
title_full_unstemmed |
Genetic Vaccination against Experimental Infection with Myotropic Parasite Strains of Trypanosoma cruzi |
title_sort |
Genetic Vaccination against Experimental Infection with Myotropic Parasite Strains of Trypanosoma cruzi |
author |
Araujo, Adriano Fernando [UNIFESP] |
author_facet |
Araujo, Adriano Fernando [UNIFESP] Oliveira, Gabriel de Vasconcelos, Juliana Fraga Ersching, Jonatan [UNIFESP] Dominguez, Mariana Ribeiro [UNIFESP] Vasconcelos, Jose Ronnie Carvalho de [UNIFESP] Machado, Alexandre Vieira Gazzinelli, Ricardo Tostes Bruna-Romero, Oscar Soares, Milena Botelho Rodrigues, Mauricio Martins [UNIFESP] |
author_role |
author |
author2 |
Oliveira, Gabriel de Vasconcelos, Juliana Fraga Ersching, Jonatan [UNIFESP] Dominguez, Mariana Ribeiro [UNIFESP] Vasconcelos, Jose Ronnie Carvalho de [UNIFESP] Machado, Alexandre Vieira Gazzinelli, Ricardo Tostes Bruna-Romero, Oscar Soares, Milena Botelho Rodrigues, Mauricio Martins [UNIFESP] |
author2_role |
author author author author author author author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Fiocruz MS Hosp Sao Rafael Universidade Federal de Minas Gerais (UFMG) Univ Massachusetts Universidade Federal de Santa Catarina (UFSC) |
dc.contributor.author.fl_str_mv |
Araujo, Adriano Fernando [UNIFESP] Oliveira, Gabriel de Vasconcelos, Juliana Fraga Ersching, Jonatan [UNIFESP] Dominguez, Mariana Ribeiro [UNIFESP] Vasconcelos, Jose Ronnie Carvalho de [UNIFESP] Machado, Alexandre Vieira Gazzinelli, Ricardo Tostes Bruna-Romero, Oscar Soares, Milena Botelho Rodrigues, Mauricio Martins [UNIFESP] |
description |
In earlier studies, we reported that a heterologous prime-boost regimen using recombinant plasmid DNA followed by replication-defective adenovirus vector, both containing Trypanosoma cruzi genes encoding trans-sialidase (TS) and amastigote surface protein (ASP) 2, provided protective immunity against experimental infection with a reticulotropic strain of this human protozoan parasite. Herein, we tested the outcome of genetic vaccination of F1 (CB10XBALB/c) mice challenged with myotropic parasite strains (Brazil and Colombian). Initially, we determined that the coadministration during priming of a DNA plasmid containing the murine IL-12 gene improved the immune response and was essential for protective immunity elicited by the heterologous prime-boost regimen in susceptible male mice against acute lethal infections with these parasites. the prophylactic or therapeutic vaccination of resistant female mice led to a drastic reduction in the number of inflammatory infiltrates in cardiac and skeletal muscles during the chronic phase of infection with either strain. Analysis of the electrocardiographic parameters showed that prophylactic vaccination reduced the frequencies of sinus arrhythmia and atrioventricular block. Our results confirmed that prophylactic vaccination using the TS and ASP-2 genes benefits the host against acute and chronic pathologies caused by T. cruzi and should be further evaluated for the development of a veterinary or human vaccine against Chagas disease. |
publishDate |
2014 |
dc.date.issued.fl_str_mv |
2014-01-01 |
dc.date.accessioned.fl_str_mv |
2016-01-24T14:34:56Z |
dc.date.available.fl_str_mv |
2016-01-24T14:34:56Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Mediators of Inflammation. New York: Hindawi Publishing Corporation, 13 p., 2014. |
dc.identifier.uri.fl_str_mv |
https://repositorio.unifesp.br/handle/11600/37146 https://dx.doi.org/10.1155/2014/605023 |
dc.identifier.issn.none.fl_str_mv |
0962-9351 |
dc.identifier.file.none.fl_str_mv |
WOS000338549100001.pdf |
dc.identifier.doi.none.fl_str_mv |
10.1155/2014/605023 |
dc.identifier.wos.none.fl_str_mv |
WOS:000338549100001 |
identifier_str_mv |
Mediators of Inflammation. New York: Hindawi Publishing Corporation, 13 p., 2014. 0962-9351 WOS000338549100001.pdf 10.1155/2014/605023 WOS:000338549100001 |
url |
https://repositorio.unifesp.br/handle/11600/37146 https://dx.doi.org/10.1155/2014/605023 |
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eng |
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eng |
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Mediators of Inflammation |
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openAccess |
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13 f. |
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Hindawi Publishing Corporation |
publisher.none.fl_str_mv |
Hindawi Publishing Corporation |
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