Macrophages and apoptosis in the stellate reticulum of the rat enamel organ

Detalhes bibliográficos
Autor(a) principal: Baratella, Liriane [UNIFESP]
Data de Publicação: 2000
Outros Autores: Arana-Chavez, V. E., Katchburian, Eduardo [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://repositorio.unifesp.br/handle/11600/26348
http://dx.doi.org/10.1046/j.1469-7580.2000.19720303.x
Resumo: Tooth germs of upper first molars of 1, 3, and 5-d-old rats, fixed in formaldehyde, were stained for the detection of apoptosis by the TUNEL method, and by the ate-dye method for the demonstration of acid phosphatase. for conventional light and electron microscopy the specimens were fixed in glutaraldehyde-formaldehyde and embedded in glycol methacrylate and Araldite. Results showed that macrophages, present in the stellate reticulum, contained basophilic bodies and TUNEL-positive globules, i.e. apoptotic bodies, in their interior. Macrophages also possessed strong acid phosphatase activity. Electron microscopy showed the presence of large vacuoles inside the macrophages containing dense fragmented material. Taken together these results suggest that the intra-epithelial macrophages of the stellate reticulum engulf apoptotic bodies.
id UFSP_483b60ce3c1289265bad200616934760
oai_identifier_str oai:repositorio.unifesp.br:11600/26348
network_acronym_str UFSP
network_name_str Repositório Institucional da UNIFESP
repository_id_str 3465
spelling Baratella, Liriane [UNIFESP]Arana-Chavez, V. E.Katchburian, Eduardo [UNIFESP]Universidade Federal de São Paulo (UNIFESP)Universidade de São Paulo (USP)Univ Ponta Grossa2016-01-24T12:31:08Z2016-01-24T12:31:08Z2000-08-01Journal of Anatomy. New York: Cambridge Univ Press, v. 197, p. 303-306, 2000.0021-8782http://repositorio.unifesp.br/handle/11600/26348http://dx.doi.org/10.1046/j.1469-7580.2000.19720303.x10.1046/j.1469-7580.2000.19720303.xWOS:000089306100015Tooth germs of upper first molars of 1, 3, and 5-d-old rats, fixed in formaldehyde, were stained for the detection of apoptosis by the TUNEL method, and by the ate-dye method for the demonstration of acid phosphatase. for conventional light and electron microscopy the specimens were fixed in glutaraldehyde-formaldehyde and embedded in glycol methacrylate and Araldite. Results showed that macrophages, present in the stellate reticulum, contained basophilic bodies and TUNEL-positive globules, i.e. apoptotic bodies, in their interior. Macrophages also possessed strong acid phosphatase activity. Electron microscopy showed the presence of large vacuoles inside the macrophages containing dense fragmented material. Taken together these results suggest that the intra-epithelial macrophages of the stellate reticulum engulf apoptotic bodies.Universidade Federal de São Paulo, Sch Med, Dept Morphol, BR-04023900 São Paulo, BrazilUniv São Paulo, Inst Biomed Sci, Dept Histol & Embryol, São Paulo, BrazilUniv Ponta Grossa, Dept Biol, Ponta Grossa, PR USAUniversidade Federal de São Paulo, Sch Med, Dept Morphol, BR-04023900 São Paulo, BrazilWeb of Science303-306engCambridge Univ PressJournal of Anatomyhttp://journals.cambridge.org/action/displaySpecialPage?pageId=4676info:eu-repo/semantics/openAccesstooth germamelogenesisodontogenesisenamelMacrophages and apoptosis in the stellate reticulum of the rat enamel organinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlereponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP11600/263482022-11-04 14:22:09.646metadata only accessoai:repositorio.unifesp.br:11600/26348Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652022-11-04T17:22:09Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.en.fl_str_mv Macrophages and apoptosis in the stellate reticulum of the rat enamel organ
title Macrophages and apoptosis in the stellate reticulum of the rat enamel organ
spellingShingle Macrophages and apoptosis in the stellate reticulum of the rat enamel organ
Baratella, Liriane [UNIFESP]
tooth germ
amelogenesis
odontogenesis
enamel
title_short Macrophages and apoptosis in the stellate reticulum of the rat enamel organ
title_full Macrophages and apoptosis in the stellate reticulum of the rat enamel organ
title_fullStr Macrophages and apoptosis in the stellate reticulum of the rat enamel organ
title_full_unstemmed Macrophages and apoptosis in the stellate reticulum of the rat enamel organ
title_sort Macrophages and apoptosis in the stellate reticulum of the rat enamel organ
author Baratella, Liriane [UNIFESP]
author_facet Baratella, Liriane [UNIFESP]
Arana-Chavez, V. E.
Katchburian, Eduardo [UNIFESP]
author_role author
author2 Arana-Chavez, V. E.
Katchburian, Eduardo [UNIFESP]
author2_role author
author
dc.contributor.institution.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Univ Ponta Grossa
dc.contributor.author.fl_str_mv Baratella, Liriane [UNIFESP]
Arana-Chavez, V. E.
Katchburian, Eduardo [UNIFESP]
dc.subject.eng.fl_str_mv tooth germ
amelogenesis
odontogenesis
enamel
topic tooth germ
amelogenesis
odontogenesis
enamel
description Tooth germs of upper first molars of 1, 3, and 5-d-old rats, fixed in formaldehyde, were stained for the detection of apoptosis by the TUNEL method, and by the ate-dye method for the demonstration of acid phosphatase. for conventional light and electron microscopy the specimens were fixed in glutaraldehyde-formaldehyde and embedded in glycol methacrylate and Araldite. Results showed that macrophages, present in the stellate reticulum, contained basophilic bodies and TUNEL-positive globules, i.e. apoptotic bodies, in their interior. Macrophages also possessed strong acid phosphatase activity. Electron microscopy showed the presence of large vacuoles inside the macrophages containing dense fragmented material. Taken together these results suggest that the intra-epithelial macrophages of the stellate reticulum engulf apoptotic bodies.
publishDate 2000
dc.date.issued.fl_str_mv 2000-08-01
dc.date.accessioned.fl_str_mv 2016-01-24T12:31:08Z
dc.date.available.fl_str_mv 2016-01-24T12:31:08Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv Journal of Anatomy. New York: Cambridge Univ Press, v. 197, p. 303-306, 2000.
dc.identifier.uri.fl_str_mv http://repositorio.unifesp.br/handle/11600/26348
http://dx.doi.org/10.1046/j.1469-7580.2000.19720303.x
dc.identifier.issn.none.fl_str_mv 0021-8782
dc.identifier.doi.none.fl_str_mv 10.1046/j.1469-7580.2000.19720303.x
dc.identifier.wos.none.fl_str_mv WOS:000089306100015
identifier_str_mv Journal of Anatomy. New York: Cambridge Univ Press, v. 197, p. 303-306, 2000.
0021-8782
10.1046/j.1469-7580.2000.19720303.x
WOS:000089306100015
url http://repositorio.unifesp.br/handle/11600/26348
http://dx.doi.org/10.1046/j.1469-7580.2000.19720303.x
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.none.fl_str_mv Journal of Anatomy
dc.rights.driver.fl_str_mv http://journals.cambridge.org/action/displaySpecialPage?pageId=4676
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://journals.cambridge.org/action/displaySpecialPage?pageId=4676
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 303-306
dc.publisher.none.fl_str_mv Cambridge Univ Press
publisher.none.fl_str_mv Cambridge Univ Press
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv
_version_ 1802764181144862720

Registros relacionados