Proteomic study revealed cellular assembly and lipid metabolism dysregulation in sepsis secondary to community-acquired pneumonia
Autor(a) principal: | |
---|---|
Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | https://repositorio.unifesp.br/handle/11600/58170 http://dx.doi.org/10.1038/s41598-017-15755-1 |
Resumo: | Sepsis is a life-threatening disorder characterized by organ dysfunction and a major cause of mortality worldwide. The major challenge in studying sepsis is its diversity in such factors as age, source of infection and etiology. Recently, genomic and proteomic approaches have improved our understanding of its complex pathogenesis. In the present study, we use quantitative proteomics to evaluate the host proteome response in septic patients secondary to community-acquired pneumonia (CAP). Samples obtained at admission and after 7 days of follow-up were analyzed according to the outcomes of septic patients. The patients' proteome profiles were compared with age-and gender-matched healthy volunteers. Bioinformatic analyses of differentially expressed proteins showed alteration in the cytoskeleton, cellular assembly, movement, lipid metabolism and immune responses in septic patients. Actin and gelsolin changes were assessed in mononuclear cells using immunofluorescence, and a higher expression of gelsolin and depletion of actin were observed in survivor patients. Regarding lipid metabolism, changes in cholesterol, HDL and apolipoproteins were confirmed using enzymatic colorimetric methods in plasma. Transcriptomic studies revealed a massive change in gene expression in sepsis. Our proteomic results stressed important changes in cellular structure and metabolism, which are possible targets for future interventions of sepsis. |
id |
UFSP_4c55722884c10b8087079b0627507c74 |
---|---|
oai_identifier_str |
oai:repositorio.unifesp.br:11600/58170 |
network_acronym_str |
UFSP |
network_name_str |
Repositório Institucional da UNIFESP |
repository_id_str |
3465 |
spelling |
Sharma, Narendra Kumar [UNIFESP]Tashima, Alexandre Keiji [UNIFESP]Colo Brunialti, Milena Karina [UNIFESP]Ferreira, Eden Ramalho [UNIFESP]Soares Torquato, Ricardo Jose [UNIFESP]Mortara, Renato Arruda [UNIFESP]Machado, Flavia Ribeiro [UNIFESP]Assuncao, MurilloRigato, Otelo [UNIFESP]Salomao, Reinaldo [UNIFESP]2020-09-01T13:21:17Z2020-09-01T13:21:17Z2017Scientific Reports. London, v. 7, p. -, 2017.2045-2322https://repositorio.unifesp.br/handle/11600/58170http://dx.doi.org/10.1038/s41598-017-15755-1WOS000415265800017.pdf10.1038/s41598-017-15755-1WOS:000415265800017Sepsis is a life-threatening disorder characterized by organ dysfunction and a major cause of mortality worldwide. The major challenge in studying sepsis is its diversity in such factors as age, source of infection and etiology. Recently, genomic and proteomic approaches have improved our understanding of its complex pathogenesis. In the present study, we use quantitative proteomics to evaluate the host proteome response in septic patients secondary to community-acquired pneumonia (CAP). Samples obtained at admission and after 7 days of follow-up were analyzed according to the outcomes of septic patients. The patients' proteome profiles were compared with age-and gender-matched healthy volunteers. Bioinformatic analyses of differentially expressed proteins showed alteration in the cytoskeleton, cellular assembly, movement, lipid metabolism and immune responses in septic patients. Actin and gelsolin changes were assessed in mononuclear cells using immunofluorescence, and a higher expression of gelsolin and depletion of actin were observed in survivor patients. Regarding lipid metabolism, changes in cholesterol, HDL and apolipoproteins were confirmed using enzymatic colorimetric methods in plasma. Transcriptomic studies revealed a massive change in gene expression in sepsis. Our proteomic results stressed important changes in cellular structure and metabolism, which are possible targets for future interventions of sepsis.Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)Conselho Nacional de Desenvolvimento Cientifico e Tecnologico, CNPqFAPESPUniv Fed Sao Paulo, Hosp Sao Paulo, Div Infect Dis, Escola Paulista Med, BR-04039032 Sao Paulo, BrazilUniv Fed Sao Paulo, Dept Biochem, Escola Paulista Med, BR-04023900 Sao Paulo, SP, BrazilUniv Fed Sao Paulo, Dept Microbiol Immunol & Parasitol, Escola Paulista Med, BR-04023062 Sao Paulo, BrazilUniv Fed Sao Paulo, Intens Care Unit, Hosp Sao Paulo, Escola Paulista Med, BR-04024002 Sao Paulo, BrazilHosp Israelita Albert Einstein, Intens Care Unit, BR-05652900 Sao Paulo, BrazilHosp Sirio Libanes, Intens Care Unit, BR-01409001 Sao Paulo, BrazilUniv Fed Sao Paulo, Hosp Sao Paulo, Div Infect Dis, Escola Paulista Med, BR-04039032 Sao Paulo, BrazilUniv Fed Sao Paulo, Dept Biochem, Escola Paulista Med, BR-04023900 Sao Paulo, SP, BrazilUniv Fed Sao Paulo, Dept Microbiol Immunol & Parasitol, Escola Paulista Med, BR-04023062 Sao Paulo, BrazilUniv Fed Sao Paulo, Intens Care Unit, Hosp Sao Paulo, Escola Paulista Med, BR-04024002 Sao Paulo, BrazilFAPESP: 2011/20401-4FAPESP: 2013/15636-8CNPq: 305685/2011-2Web of Science-engNature Publishing GroupScientific ReportsProteomic study revealed cellular assembly and lipid metabolism dysregulation in sepsis secondary to community-acquired pneumoniainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleLondon7info:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000415265800017.pdfapplication/pdf4788359${dspace.ui.url}/bitstream/11600/58170/1/WOS000415265800017.pdf8a22954b76e9e0b4749b5ddf32f64134MD51open accessTEXTWOS000415265800017.pdf.txtWOS000415265800017.pdf.txtExtracted texttext/plain57492${dspace.ui.url}/bitstream/11600/58170/2/WOS000415265800017.pdf.txte9a0250d1e86fbef2697fa512131affcMD52open accessTHUMBNAILWOS000415265800017.pdf.jpgWOS000415265800017.pdf.jpgIM Thumbnailimage/jpeg7534${dspace.ui.url}/bitstream/11600/58170/4/WOS000415265800017.pdf.jpgc9b5c6316434a0296697dd02143f2419MD54open access11600/581702022-07-31 18:47:04.447open accessoai:repositorio.unifesp.br:11600/58170Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652022-07-31T21:47:04Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Proteomic study revealed cellular assembly and lipid metabolism dysregulation in sepsis secondary to community-acquired pneumonia |
title |
Proteomic study revealed cellular assembly and lipid metabolism dysregulation in sepsis secondary to community-acquired pneumonia |
spellingShingle |
Proteomic study revealed cellular assembly and lipid metabolism dysregulation in sepsis secondary to community-acquired pneumonia Sharma, Narendra Kumar [UNIFESP] |
title_short |
Proteomic study revealed cellular assembly and lipid metabolism dysregulation in sepsis secondary to community-acquired pneumonia |
title_full |
Proteomic study revealed cellular assembly and lipid metabolism dysregulation in sepsis secondary to community-acquired pneumonia |
title_fullStr |
Proteomic study revealed cellular assembly and lipid metabolism dysregulation in sepsis secondary to community-acquired pneumonia |
title_full_unstemmed |
Proteomic study revealed cellular assembly and lipid metabolism dysregulation in sepsis secondary to community-acquired pneumonia |
title_sort |
Proteomic study revealed cellular assembly and lipid metabolism dysregulation in sepsis secondary to community-acquired pneumonia |
author |
Sharma, Narendra Kumar [UNIFESP] |
author_facet |
Sharma, Narendra Kumar [UNIFESP] Tashima, Alexandre Keiji [UNIFESP] Colo Brunialti, Milena Karina [UNIFESP] Ferreira, Eden Ramalho [UNIFESP] Soares Torquato, Ricardo Jose [UNIFESP] Mortara, Renato Arruda [UNIFESP] Machado, Flavia Ribeiro [UNIFESP] Assuncao, Murillo Rigato, Otelo [UNIFESP] Salomao, Reinaldo [UNIFESP] |
author_role |
author |
author2 |
Tashima, Alexandre Keiji [UNIFESP] Colo Brunialti, Milena Karina [UNIFESP] Ferreira, Eden Ramalho [UNIFESP] Soares Torquato, Ricardo Jose [UNIFESP] Mortara, Renato Arruda [UNIFESP] Machado, Flavia Ribeiro [UNIFESP] Assuncao, Murillo Rigato, Otelo [UNIFESP] Salomao, Reinaldo [UNIFESP] |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Sharma, Narendra Kumar [UNIFESP] Tashima, Alexandre Keiji [UNIFESP] Colo Brunialti, Milena Karina [UNIFESP] Ferreira, Eden Ramalho [UNIFESP] Soares Torquato, Ricardo Jose [UNIFESP] Mortara, Renato Arruda [UNIFESP] Machado, Flavia Ribeiro [UNIFESP] Assuncao, Murillo Rigato, Otelo [UNIFESP] Salomao, Reinaldo [UNIFESP] |
description |
Sepsis is a life-threatening disorder characterized by organ dysfunction and a major cause of mortality worldwide. The major challenge in studying sepsis is its diversity in such factors as age, source of infection and etiology. Recently, genomic and proteomic approaches have improved our understanding of its complex pathogenesis. In the present study, we use quantitative proteomics to evaluate the host proteome response in septic patients secondary to community-acquired pneumonia (CAP). Samples obtained at admission and after 7 days of follow-up were analyzed according to the outcomes of septic patients. The patients' proteome profiles were compared with age-and gender-matched healthy volunteers. Bioinformatic analyses of differentially expressed proteins showed alteration in the cytoskeleton, cellular assembly, movement, lipid metabolism and immune responses in septic patients. Actin and gelsolin changes were assessed in mononuclear cells using immunofluorescence, and a higher expression of gelsolin and depletion of actin were observed in survivor patients. Regarding lipid metabolism, changes in cholesterol, HDL and apolipoproteins were confirmed using enzymatic colorimetric methods in plasma. Transcriptomic studies revealed a massive change in gene expression in sepsis. Our proteomic results stressed important changes in cellular structure and metabolism, which are possible targets for future interventions of sepsis. |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017 |
dc.date.accessioned.fl_str_mv |
2020-09-01T13:21:17Z |
dc.date.available.fl_str_mv |
2020-09-01T13:21:17Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Scientific Reports. London, v. 7, p. -, 2017. |
dc.identifier.uri.fl_str_mv |
https://repositorio.unifesp.br/handle/11600/58170 http://dx.doi.org/10.1038/s41598-017-15755-1 |
dc.identifier.issn.none.fl_str_mv |
2045-2322 |
dc.identifier.file.none.fl_str_mv |
WOS000415265800017.pdf |
dc.identifier.doi.none.fl_str_mv |
10.1038/s41598-017-15755-1 |
dc.identifier.wos.none.fl_str_mv |
WOS:000415265800017 |
identifier_str_mv |
Scientific Reports. London, v. 7, p. -, 2017. 2045-2322 WOS000415265800017.pdf 10.1038/s41598-017-15755-1 WOS:000415265800017 |
url |
https://repositorio.unifesp.br/handle/11600/58170 http://dx.doi.org/10.1038/s41598-017-15755-1 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Scientific Reports |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
- |
dc.coverage.none.fl_str_mv |
London |
dc.publisher.none.fl_str_mv |
Nature Publishing Group |
publisher.none.fl_str_mv |
Nature Publishing Group |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
bitstream.url.fl_str_mv |
${dspace.ui.url}/bitstream/11600/58170/1/WOS000415265800017.pdf ${dspace.ui.url}/bitstream/11600/58170/2/WOS000415265800017.pdf.txt ${dspace.ui.url}/bitstream/11600/58170/4/WOS000415265800017.pdf.jpg |
bitstream.checksum.fl_str_mv |
8a22954b76e9e0b4749b5ddf32f64134 e9a0250d1e86fbef2697fa512131affc c9b5c6316434a0296697dd02143f2419 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
|
_version_ |
1802764161134886912 |