Poly(lactic acid-glycolic acid) nanoparticles markedly improve immunological protection provided by peptide P10 against murine paracoccidioidomycosis
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/32293 http://dx.doi.org/10.1111/j.1476-5381.2009.00617.x |
Resumo: | Background and purpose:The present study reports on the preparation and testing of a sustained delivery system for the immunomodulatory peptide P10 aimed at reducing the in vivo degradation of the peptide and the amount required to elicit a protective immune response against paracoccidioidomycosis.Experimental approach:BALB/c mice were infected with the yeast Paracoccidioides brasiliensis to mimic the chronic form of paracoccidioidomycosis. the animals were treated daily with sulfamethoxazole/trimethoprim alone or combined with peptide P10, either emulsified in Freund's adjuvant or entrapped in poly(lactic acid-glycolic acid) (PLGA) nanoparticles at different concentrations (1 mu g, 5 mu g, 10 mu g, 20 mu g or 40 mu g center dot 50 mu L-1). Therapeutic efficacy was assessed as fungal burden in tissues and the immune response by quantitative determination of cytokines.Key results:Animals given combined chemotherapy and P10 nanotherapy presented a marked reduction of fungal load in the lungs, compared with the non-treated animals. After 30 days of treatment, P10 entrapped within PLGA (1 mu g center dot 50 mu L-1) was more effective than 'free' P10 emulsified in Freund's adjuvant (20 mu g center dot 50 mu L-1), as an adjuvant to chemotherapy. After treatment for 90 days, the higher doses of P10 entrapped within PLGA (5 or 10 mu g center dot 50 mu L-1) were most effective. Treatment with P10 emulsified in Freund's adjuvant (20 mu g center dot 50 mu L-1) or P10 entrapped within PLGA (1 mu g center dot 50 mu L-1) were accompanied by high levels of interferon-gamma in lung.Conclusions and implications:Combination of sulfamethoxazole/trimethoprim with the P10 peptide entrapped within PLGA demonstrated increased therapeutic efficacy against paracoccidioidomycosis. P10 incorporation into PLGA nanoparticles dramatically reduced the peptide amount necessary to elicit a protective effect. |
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Amaral, Andre C.Marques, Alexandre F.Munoz, Julian E.Bocca, Anamelia L.Simioni, Andreza R.Tedesco, Antonio C.Morais, Paulo C.Travassos, Luiz Rodolpho [UNIFESP]Taborda, Carlos Pelleschi [UNIFESP]Felipe, Maria Sueli S.Universidade de Brasília (UnB)Univ Catolica BrasiliaUniversidade de São Paulo (USP)Universidade Federal de São Paulo (UNIFESP)2016-01-24T13:59:21Z2016-01-24T13:59:21Z2010-03-01British Journal of Pharmacology. Malden: Wiley-Blackwell Publishing, Inc, v. 159, n. 5, p. 1126-1132, 2010.0007-1188http://repositorio.unifesp.br/handle/11600/32293http://dx.doi.org/10.1111/j.1476-5381.2009.00617.x10.1111/j.1476-5381.2009.00617.xWOS:000275402000014Background and purpose:The present study reports on the preparation and testing of a sustained delivery system for the immunomodulatory peptide P10 aimed at reducing the in vivo degradation of the peptide and the amount required to elicit a protective immune response against paracoccidioidomycosis.Experimental approach:BALB/c mice were infected with the yeast Paracoccidioides brasiliensis to mimic the chronic form of paracoccidioidomycosis. the animals were treated daily with sulfamethoxazole/trimethoprim alone or combined with peptide P10, either emulsified in Freund's adjuvant or entrapped in poly(lactic acid-glycolic acid) (PLGA) nanoparticles at different concentrations (1 mu g, 5 mu g, 10 mu g, 20 mu g or 40 mu g center dot 50 mu L-1). Therapeutic efficacy was assessed as fungal burden in tissues and the immune response by quantitative determination of cytokines.Key results:Animals given combined chemotherapy and P10 nanotherapy presented a marked reduction of fungal load in the lungs, compared with the non-treated animals. After 30 days of treatment, P10 entrapped within PLGA (1 mu g center dot 50 mu L-1) was more effective than 'free' P10 emulsified in Freund's adjuvant (20 mu g center dot 50 mu L-1), as an adjuvant to chemotherapy. After treatment for 90 days, the higher doses of P10 entrapped within PLGA (5 or 10 mu g center dot 50 mu L-1) were most effective. Treatment with P10 emulsified in Freund's adjuvant (20 mu g center dot 50 mu L-1) or P10 entrapped within PLGA (1 mu g center dot 50 mu L-1) were accompanied by high levels of interferon-gamma in lung.Conclusions and implications:Combination of sulfamethoxazole/trimethoprim with the P10 peptide entrapped within PLGA demonstrated increased therapeutic efficacy against paracoccidioidomycosis. P10 incorporation into PLGA nanoparticles dramatically reduced the peptide amount necessary to elicit a protective effect.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Brasilia, Dept Biol Celular, Mol Biol Lab, Inst Biol Sci, BR-70910900 Brasilia, DF, BrazilUniv Brasilia, Inst Phys, BR-70910900 Brasilia, DF, BrazilUniv Catolica Brasilia, Brasilia, DF, BrazilUniv São Paulo, Inst Chem, BR-14049 Ribeirao Preto, BrazilUniv São Paulo, Inst Biomed Sci, São Paulo, BrazilUniv São Paulo, Med Mycol Lab, LIM53, MTSP, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, São Paulo, BrazilWeb of Science1126-1132engWiley-BlackwellBritish Journal of Pharmacologyhttp://olabout.wiley.com/WileyCDA/Section/id-406071.htmlinfo:eu-repo/semantics/openAccessimmunomodulatory peptideantifungal therapybiodegradable polymersdrug deliverynanobiotechnologyPoly(lactic acid-glycolic acid) nanoparticles markedly improve immunological protection provided by peptide P10 against murine paracoccidioidomycosisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlereponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP11600/322932022-11-04 15:08:28.744metadata only accessoai:repositorio.unifesp.br:11600/32293Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-05-25T12:28:49.401628Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Poly(lactic acid-glycolic acid) nanoparticles markedly improve immunological protection provided by peptide P10 against murine paracoccidioidomycosis |
title |
Poly(lactic acid-glycolic acid) nanoparticles markedly improve immunological protection provided by peptide P10 against murine paracoccidioidomycosis |
spellingShingle |
Poly(lactic acid-glycolic acid) nanoparticles markedly improve immunological protection provided by peptide P10 against murine paracoccidioidomycosis Amaral, Andre C. immunomodulatory peptide antifungal therapy biodegradable polymers drug delivery nanobiotechnology |
title_short |
Poly(lactic acid-glycolic acid) nanoparticles markedly improve immunological protection provided by peptide P10 against murine paracoccidioidomycosis |
title_full |
Poly(lactic acid-glycolic acid) nanoparticles markedly improve immunological protection provided by peptide P10 against murine paracoccidioidomycosis |
title_fullStr |
Poly(lactic acid-glycolic acid) nanoparticles markedly improve immunological protection provided by peptide P10 against murine paracoccidioidomycosis |
title_full_unstemmed |
Poly(lactic acid-glycolic acid) nanoparticles markedly improve immunological protection provided by peptide P10 against murine paracoccidioidomycosis |
title_sort |
Poly(lactic acid-glycolic acid) nanoparticles markedly improve immunological protection provided by peptide P10 against murine paracoccidioidomycosis |
author |
Amaral, Andre C. |
author_facet |
Amaral, Andre C. Marques, Alexandre F. Munoz, Julian E. Bocca, Anamelia L. Simioni, Andreza R. Tedesco, Antonio C. Morais, Paulo C. Travassos, Luiz Rodolpho [UNIFESP] Taborda, Carlos Pelleschi [UNIFESP] Felipe, Maria Sueli S. |
author_role |
author |
author2 |
Marques, Alexandre F. Munoz, Julian E. Bocca, Anamelia L. Simioni, Andreza R. Tedesco, Antonio C. Morais, Paulo C. Travassos, Luiz Rodolpho [UNIFESP] Taborda, Carlos Pelleschi [UNIFESP] Felipe, Maria Sueli S. |
author2_role |
author author author author author author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade de Brasília (UnB) Univ Catolica Brasilia Universidade de São Paulo (USP) Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Amaral, Andre C. Marques, Alexandre F. Munoz, Julian E. Bocca, Anamelia L. Simioni, Andreza R. Tedesco, Antonio C. Morais, Paulo C. Travassos, Luiz Rodolpho [UNIFESP] Taborda, Carlos Pelleschi [UNIFESP] Felipe, Maria Sueli S. |
dc.subject.eng.fl_str_mv |
immunomodulatory peptide antifungal therapy biodegradable polymers drug delivery nanobiotechnology |
topic |
immunomodulatory peptide antifungal therapy biodegradable polymers drug delivery nanobiotechnology |
description |
Background and purpose:The present study reports on the preparation and testing of a sustained delivery system for the immunomodulatory peptide P10 aimed at reducing the in vivo degradation of the peptide and the amount required to elicit a protective immune response against paracoccidioidomycosis.Experimental approach:BALB/c mice were infected with the yeast Paracoccidioides brasiliensis to mimic the chronic form of paracoccidioidomycosis. the animals were treated daily with sulfamethoxazole/trimethoprim alone or combined with peptide P10, either emulsified in Freund's adjuvant or entrapped in poly(lactic acid-glycolic acid) (PLGA) nanoparticles at different concentrations (1 mu g, 5 mu g, 10 mu g, 20 mu g or 40 mu g center dot 50 mu L-1). Therapeutic efficacy was assessed as fungal burden in tissues and the immune response by quantitative determination of cytokines.Key results:Animals given combined chemotherapy and P10 nanotherapy presented a marked reduction of fungal load in the lungs, compared with the non-treated animals. After 30 days of treatment, P10 entrapped within PLGA (1 mu g center dot 50 mu L-1) was more effective than 'free' P10 emulsified in Freund's adjuvant (20 mu g center dot 50 mu L-1), as an adjuvant to chemotherapy. After treatment for 90 days, the higher doses of P10 entrapped within PLGA (5 or 10 mu g center dot 50 mu L-1) were most effective. Treatment with P10 emulsified in Freund's adjuvant (20 mu g center dot 50 mu L-1) or P10 entrapped within PLGA (1 mu g center dot 50 mu L-1) were accompanied by high levels of interferon-gamma in lung.Conclusions and implications:Combination of sulfamethoxazole/trimethoprim with the P10 peptide entrapped within PLGA demonstrated increased therapeutic efficacy against paracoccidioidomycosis. P10 incorporation into PLGA nanoparticles dramatically reduced the peptide amount necessary to elicit a protective effect. |
publishDate |
2010 |
dc.date.issued.fl_str_mv |
2010-03-01 |
dc.date.accessioned.fl_str_mv |
2016-01-24T13:59:21Z |
dc.date.available.fl_str_mv |
2016-01-24T13:59:21Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
British Journal of Pharmacology. Malden: Wiley-Blackwell Publishing, Inc, v. 159, n. 5, p. 1126-1132, 2010. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/32293 http://dx.doi.org/10.1111/j.1476-5381.2009.00617.x |
dc.identifier.issn.none.fl_str_mv |
0007-1188 |
dc.identifier.doi.none.fl_str_mv |
10.1111/j.1476-5381.2009.00617.x |
dc.identifier.wos.none.fl_str_mv |
WOS:000275402000014 |
identifier_str_mv |
British Journal of Pharmacology. Malden: Wiley-Blackwell Publishing, Inc, v. 159, n. 5, p. 1126-1132, 2010. 0007-1188 10.1111/j.1476-5381.2009.00617.x WOS:000275402000014 |
url |
http://repositorio.unifesp.br/handle/11600/32293 http://dx.doi.org/10.1111/j.1476-5381.2009.00617.x |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
British Journal of Pharmacology |
dc.rights.driver.fl_str_mv |
http://olabout.wiley.com/WileyCDA/Section/id-406071.html info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://olabout.wiley.com/WileyCDA/Section/id-406071.html |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1126-1132 |
dc.publisher.none.fl_str_mv |
Wiley-Blackwell |
publisher.none.fl_str_mv |
Wiley-Blackwell |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
|
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1783460296597176320 |