Characterization of monoclonal antibody MEST-2 specific to glucosylceramide of fungi and plants
Autor(a) principal: | |
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Data de Publicação: | 2001 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/26477 http://dx.doi.org/10.1093/glycob/11.2.105 |
Resumo: | An IgG2a monoclonal antibody anti-glucosylceramide was established and termed MEST-2. High performance thin layer chromatography immunostaining, and solid-phase radioimmunoassay showed that MEST-2 reacts with glucosylceramide from yeast and mycelium forms of Paracoccidioides brasiliensis, Histoplasma capsulatum, and Sporothrix schenckii; from hyphae of Aspergillus fumigatus; and from yeast forms of Candida albicans, Cryptococcus neoformans, Cryptococcus laurentii, and Cryptococcus albidus, Studies on the fine specificity of MEST-2 showed that it recognizes the beta -D-glucose residue, and that the 2-hydroxy group present in the fatty acid is an important auxiliary feature for the antibody binding. It was also demonstrated that phosphatidylcholine and ergosterol modulate MEST-2 reactivity to glucosylceramide, by solid-phase radioimmunoassay. Indirect immunofluorescence showed that MEST-2 reacts with the surface of yeast forms of P. brasiliensis, H. capsulatum and S. schenckii. Weak staining of mycelial forms of P, brasiliensis and hyphae of A. fumigatus was also observed. the availability of a monoclonal antibody specific to fungal glucosylceramide, and its potential use in analyzing biological roles attributed to glucosylceramide in fungi are discussed. |
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Toledo, Marcos Sergio de [UNIFESP]Suzuki, Erika [UNIFESP]Levery, SBStraus, Anita Hilda [UNIFESP]Takahashi, Helio Kiyoshi [UNIFESP]Universidade Federal de São Paulo (UNIFESP)Univ Georgia2016-01-24T12:31:18Z2016-01-24T12:31:18Z2001-02-01Glycobiology. Cary: Oxford Univ Press Inc, v. 11, n. 2, p. 105-112, 2001.0959-6658http://repositorio.unifesp.br/handle/11600/26477http://dx.doi.org/10.1093/glycob/11.2.10510.1093/glycob/11.2.105WOS:000167957700004An IgG2a monoclonal antibody anti-glucosylceramide was established and termed MEST-2. High performance thin layer chromatography immunostaining, and solid-phase radioimmunoassay showed that MEST-2 reacts with glucosylceramide from yeast and mycelium forms of Paracoccidioides brasiliensis, Histoplasma capsulatum, and Sporothrix schenckii; from hyphae of Aspergillus fumigatus; and from yeast forms of Candida albicans, Cryptococcus neoformans, Cryptococcus laurentii, and Cryptococcus albidus, Studies on the fine specificity of MEST-2 showed that it recognizes the beta -D-glucose residue, and that the 2-hydroxy group present in the fatty acid is an important auxiliary feature for the antibody binding. It was also demonstrated that phosphatidylcholine and ergosterol modulate MEST-2 reactivity to glucosylceramide, by solid-phase radioimmunoassay. Indirect immunofluorescence showed that MEST-2 reacts with the surface of yeast forms of P. brasiliensis, H. capsulatum and S. schenckii. Weak staining of mycelial forms of P, brasiliensis and hyphae of A. fumigatus was also observed. the availability of a monoclonal antibody specific to fungal glucosylceramide, and its potential use in analyzing biological roles attributed to glucosylceramide in fungi are discussed.Universidade Federal de São Paulo, Escola Paulista Med, Dept Biochem, BR-04023900 São Paulo, BrazilUniv Georgia, Complex Carbohydrate Res Ctr, Athens, GA 30602 USAUniversidade Federal de São Paulo, Escola Paulista Med, Dept Biochem, BR-04023900 São Paulo, BrazilWeb of Science105-112engOxford Univ Press IncGlycobiologyhttp://www.oxfordjournals.org/access_purchase/self-archiving_policyb.htmlinfo:eu-repo/semantics/openAccess2-hydroxy fatty acidglucosylceramidemonoclonal antibodyparacoccidioides brasiliensispathogenic fungiCharacterization of monoclonal antibody MEST-2 specific to glucosylceramide of fungi and plantsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlereponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP11600/264772022-09-27 12:00:36.234metadata only accessoai:repositorio.unifesp.br:11600/26477Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-05-25T12:26:10.892663Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Characterization of monoclonal antibody MEST-2 specific to glucosylceramide of fungi and plants |
title |
Characterization of monoclonal antibody MEST-2 specific to glucosylceramide of fungi and plants |
spellingShingle |
Characterization of monoclonal antibody MEST-2 specific to glucosylceramide of fungi and plants Toledo, Marcos Sergio de [UNIFESP] 2-hydroxy fatty acid glucosylceramide monoclonal antibody paracoccidioides brasiliensis pathogenic fungi |
title_short |
Characterization of monoclonal antibody MEST-2 specific to glucosylceramide of fungi and plants |
title_full |
Characterization of monoclonal antibody MEST-2 specific to glucosylceramide of fungi and plants |
title_fullStr |
Characterization of monoclonal antibody MEST-2 specific to glucosylceramide of fungi and plants |
title_full_unstemmed |
Characterization of monoclonal antibody MEST-2 specific to glucosylceramide of fungi and plants |
title_sort |
Characterization of monoclonal antibody MEST-2 specific to glucosylceramide of fungi and plants |
author |
Toledo, Marcos Sergio de [UNIFESP] |
author_facet |
Toledo, Marcos Sergio de [UNIFESP] Suzuki, Erika [UNIFESP] Levery, SB Straus, Anita Hilda [UNIFESP] Takahashi, Helio Kiyoshi [UNIFESP] |
author_role |
author |
author2 |
Suzuki, Erika [UNIFESP] Levery, SB Straus, Anita Hilda [UNIFESP] Takahashi, Helio Kiyoshi [UNIFESP] |
author2_role |
author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Univ Georgia |
dc.contributor.author.fl_str_mv |
Toledo, Marcos Sergio de [UNIFESP] Suzuki, Erika [UNIFESP] Levery, SB Straus, Anita Hilda [UNIFESP] Takahashi, Helio Kiyoshi [UNIFESP] |
dc.subject.eng.fl_str_mv |
2-hydroxy fatty acid glucosylceramide monoclonal antibody paracoccidioides brasiliensis pathogenic fungi |
topic |
2-hydroxy fatty acid glucosylceramide monoclonal antibody paracoccidioides brasiliensis pathogenic fungi |
description |
An IgG2a monoclonal antibody anti-glucosylceramide was established and termed MEST-2. High performance thin layer chromatography immunostaining, and solid-phase radioimmunoassay showed that MEST-2 reacts with glucosylceramide from yeast and mycelium forms of Paracoccidioides brasiliensis, Histoplasma capsulatum, and Sporothrix schenckii; from hyphae of Aspergillus fumigatus; and from yeast forms of Candida albicans, Cryptococcus neoformans, Cryptococcus laurentii, and Cryptococcus albidus, Studies on the fine specificity of MEST-2 showed that it recognizes the beta -D-glucose residue, and that the 2-hydroxy group present in the fatty acid is an important auxiliary feature for the antibody binding. It was also demonstrated that phosphatidylcholine and ergosterol modulate MEST-2 reactivity to glucosylceramide, by solid-phase radioimmunoassay. Indirect immunofluorescence showed that MEST-2 reacts with the surface of yeast forms of P. brasiliensis, H. capsulatum and S. schenckii. Weak staining of mycelial forms of P, brasiliensis and hyphae of A. fumigatus was also observed. the availability of a monoclonal antibody specific to fungal glucosylceramide, and its potential use in analyzing biological roles attributed to glucosylceramide in fungi are discussed. |
publishDate |
2001 |
dc.date.issued.fl_str_mv |
2001-02-01 |
dc.date.accessioned.fl_str_mv |
2016-01-24T12:31:18Z |
dc.date.available.fl_str_mv |
2016-01-24T12:31:18Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Glycobiology. Cary: Oxford Univ Press Inc, v. 11, n. 2, p. 105-112, 2001. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/26477 http://dx.doi.org/10.1093/glycob/11.2.105 |
dc.identifier.issn.none.fl_str_mv |
0959-6658 |
dc.identifier.doi.none.fl_str_mv |
10.1093/glycob/11.2.105 |
dc.identifier.wos.none.fl_str_mv |
WOS:000167957700004 |
identifier_str_mv |
Glycobiology. Cary: Oxford Univ Press Inc, v. 11, n. 2, p. 105-112, 2001. 0959-6658 10.1093/glycob/11.2.105 WOS:000167957700004 |
url |
http://repositorio.unifesp.br/handle/11600/26477 http://dx.doi.org/10.1093/glycob/11.2.105 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Glycobiology |
dc.rights.driver.fl_str_mv |
http://www.oxfordjournals.org/access_purchase/self-archiving_policyb.html info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://www.oxfordjournals.org/access_purchase/self-archiving_policyb.html |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
105-112 |
dc.publisher.none.fl_str_mv |
Oxford Univ Press Inc |
publisher.none.fl_str_mv |
Oxford Univ Press Inc |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
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UNIFESP |
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UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
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