Behavioral and electroencephalographic analysis of seizures induced by intrahippocampal injection of granulitoxin, a neurotoxic peptide from the sea anemone Bunodosoma granulifera
Autor(a) principal: | |
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Data de Publicação: | 2001 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/1188 http://dx.doi.org/10.1590/S0100-879X2001000600016 |
Resumo: | In this study, the behavioral and electroencephalographic (EEG) analysis of seizures induced by the intrahippocampal injection in rats of granulitoxin, a neurotoxic peptide from the sea anemone Bunodosoma granulifera, was determined. The first alterations occurred during microinjection of granulitoxin (8 µg) into the dorsal hippocampus and consisted of seizure activity that began in the hippocampus and spread rapidly to the occipital cortex. This activity lasted 20-30 s, and during this period the rats presented immobility. During the first 40-50 min after its administration, three to four other similar short EEG seizure periods occurred and the rats presented the following behavioral alterations: akinesia, facial automatisms, head tremor, salivation, rearing, jumping, barrel-rolling, wet dog shakes and forelimb clonic movements. Within 40-50 min, the status epilepticus was established and lasted 8-12 h. These results are similar to those observed in the acute phase of the pilocarpine model of temporal lobe epilepsy and suggest that granulitoxin may be a useful tool not only to study the sodium channels, but also to develop a new experimental model of status epilepticus. |
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Santana, Alfredo Nicodemus da CruzTrindade Filho, Euclides Mauricio [UNIFESP]Cunha, R.b.Sousa, M.v.Cavalheiro, Esper Abrão [UNIFESP]Carvalho, K.m.Universidade Estadual do Ceará Departamento de Ciências Fisiológicas Laboratório de NeurofarmacologiaUniversidade Federal de São Paulo (UNIFESP)Universidade de Brasília Departamento de Biologia Celular Centro Brasileiro de Serviços e Pesquisas em Proteínas2015-06-14T13:29:26Z2015-06-14T13:29:26Z2001-06-01Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 34, n. 6, p. 797-801, 2001.0100-879Xhttp://repositorio.unifesp.br/handle/11600/1188http://dx.doi.org/10.1590/S0100-879X2001000600016S0100-879X2001000600016.pdfS0100-879X200100060001610.1590/S0100-879X2001000600016WOS:000169443400016In this study, the behavioral and electroencephalographic (EEG) analysis of seizures induced by the intrahippocampal injection in rats of granulitoxin, a neurotoxic peptide from the sea anemone Bunodosoma granulifera, was determined. The first alterations occurred during microinjection of granulitoxin (8 µg) into the dorsal hippocampus and consisted of seizure activity that began in the hippocampus and spread rapidly to the occipital cortex. This activity lasted 20-30 s, and during this period the rats presented immobility. During the first 40-50 min after its administration, three to four other similar short EEG seizure periods occurred and the rats presented the following behavioral alterations: akinesia, facial automatisms, head tremor, salivation, rearing, jumping, barrel-rolling, wet dog shakes and forelimb clonic movements. Within 40-50 min, the status epilepticus was established and lasted 8-12 h. These results are similar to those observed in the acute phase of the pilocarpine model of temporal lobe epilepsy and suggest that granulitoxin may be a useful tool not only to study the sodium channels, but also to develop a new experimental model of status epilepticus.Universidade Estadual do Ceará Departamento de Ciências Fisiológicas Laboratório de NeurofarmacologiaUniversidade Federal de São Paulo (UNIFESP) Departamento de Neurologia e Neurocirurgia Laboratório de Neurologia ExperimentalUniversidade de Brasília Departamento de Biologia Celular Centro Brasileiro de Serviços e Pesquisas em ProteínasUNIFESP, Depto. de Neurologia e Neurocirurgia Laboratório de Neurologia ExperimentalSciELO797-801engAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological ResearchBunodosoma granuliferagranulitoxinsea anemonestoxic peptidesseizureelectroencephalographic assayBehavioral and electroencephalographic analysis of seizures induced by intrahippocampal injection of granulitoxin, a neurotoxic peptide from the sea anemone Bunodosoma granuliferainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALS0100-879X2001000600016.pdfapplication/pdf142176${dspace.ui.url}/bitstream/11600/1188/1/S0100-879X2001000600016.pdf6fe5fc6e766dff37c65a2a1ed1232467MD51open accessTEXTS0100-879X2001000600016.pdf.txtS0100-879X2001000600016.pdf.txtExtracted texttext/plain17567${dspace.ui.url}/bitstream/11600/1188/2/S0100-879X2001000600016.pdf.txte5e55afef73ed07e9a83d14b6787ffd7MD52open access11600/11882021-09-30 11:01:06.809open accessoai:repositorio.unifesp.br:11600/1188Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-05-25T12:25:21.487809Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Behavioral and electroencephalographic analysis of seizures induced by intrahippocampal injection of granulitoxin, a neurotoxic peptide from the sea anemone Bunodosoma granulifera |
title |
Behavioral and electroencephalographic analysis of seizures induced by intrahippocampal injection of granulitoxin, a neurotoxic peptide from the sea anemone Bunodosoma granulifera |
spellingShingle |
Behavioral and electroencephalographic analysis of seizures induced by intrahippocampal injection of granulitoxin, a neurotoxic peptide from the sea anemone Bunodosoma granulifera Santana, Alfredo Nicodemus da Cruz Bunodosoma granulifera granulitoxin sea anemones toxic peptides seizure electroencephalographic assay |
title_short |
Behavioral and electroencephalographic analysis of seizures induced by intrahippocampal injection of granulitoxin, a neurotoxic peptide from the sea anemone Bunodosoma granulifera |
title_full |
Behavioral and electroencephalographic analysis of seizures induced by intrahippocampal injection of granulitoxin, a neurotoxic peptide from the sea anemone Bunodosoma granulifera |
title_fullStr |
Behavioral and electroencephalographic analysis of seizures induced by intrahippocampal injection of granulitoxin, a neurotoxic peptide from the sea anemone Bunodosoma granulifera |
title_full_unstemmed |
Behavioral and electroencephalographic analysis of seizures induced by intrahippocampal injection of granulitoxin, a neurotoxic peptide from the sea anemone Bunodosoma granulifera |
title_sort |
Behavioral and electroencephalographic analysis of seizures induced by intrahippocampal injection of granulitoxin, a neurotoxic peptide from the sea anemone Bunodosoma granulifera |
author |
Santana, Alfredo Nicodemus da Cruz |
author_facet |
Santana, Alfredo Nicodemus da Cruz Trindade Filho, Euclides Mauricio [UNIFESP] Cunha, R.b. Sousa, M.v. Cavalheiro, Esper Abrão [UNIFESP] Carvalho, K.m. |
author_role |
author |
author2 |
Trindade Filho, Euclides Mauricio [UNIFESP] Cunha, R.b. Sousa, M.v. Cavalheiro, Esper Abrão [UNIFESP] Carvalho, K.m. |
author2_role |
author author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Estadual do Ceará Departamento de Ciências Fisiológicas Laboratório de Neurofarmacologia Universidade Federal de São Paulo (UNIFESP) Universidade de Brasília Departamento de Biologia Celular Centro Brasileiro de Serviços e Pesquisas em Proteínas |
dc.contributor.author.fl_str_mv |
Santana, Alfredo Nicodemus da Cruz Trindade Filho, Euclides Mauricio [UNIFESP] Cunha, R.b. Sousa, M.v. Cavalheiro, Esper Abrão [UNIFESP] Carvalho, K.m. |
dc.subject.eng.fl_str_mv |
Bunodosoma granulifera granulitoxin sea anemones toxic peptides seizure electroencephalographic assay |
topic |
Bunodosoma granulifera granulitoxin sea anemones toxic peptides seizure electroencephalographic assay |
description |
In this study, the behavioral and electroencephalographic (EEG) analysis of seizures induced by the intrahippocampal injection in rats of granulitoxin, a neurotoxic peptide from the sea anemone Bunodosoma granulifera, was determined. The first alterations occurred during microinjection of granulitoxin (8 µg) into the dorsal hippocampus and consisted of seizure activity that began in the hippocampus and spread rapidly to the occipital cortex. This activity lasted 20-30 s, and during this period the rats presented immobility. During the first 40-50 min after its administration, three to four other similar short EEG seizure periods occurred and the rats presented the following behavioral alterations: akinesia, facial automatisms, head tremor, salivation, rearing, jumping, barrel-rolling, wet dog shakes and forelimb clonic movements. Within 40-50 min, the status epilepticus was established and lasted 8-12 h. These results are similar to those observed in the acute phase of the pilocarpine model of temporal lobe epilepsy and suggest that granulitoxin may be a useful tool not only to study the sodium channels, but also to develop a new experimental model of status epilepticus. |
publishDate |
2001 |
dc.date.issued.fl_str_mv |
2001-06-01 |
dc.date.accessioned.fl_str_mv |
2015-06-14T13:29:26Z |
dc.date.available.fl_str_mv |
2015-06-14T13:29:26Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 34, n. 6, p. 797-801, 2001. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/1188 http://dx.doi.org/10.1590/S0100-879X2001000600016 |
dc.identifier.issn.none.fl_str_mv |
0100-879X |
dc.identifier.file.none.fl_str_mv |
S0100-879X2001000600016.pdf |
dc.identifier.scielo.none.fl_str_mv |
S0100-879X2001000600016 |
dc.identifier.doi.none.fl_str_mv |
10.1590/S0100-879X2001000600016 |
dc.identifier.wos.none.fl_str_mv |
WOS:000169443400016 |
identifier_str_mv |
Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 34, n. 6, p. 797-801, 2001. 0100-879X S0100-879X2001000600016.pdf S0100-879X2001000600016 10.1590/S0100-879X2001000600016 WOS:000169443400016 |
url |
http://repositorio.unifesp.br/handle/11600/1188 http://dx.doi.org/10.1590/S0100-879X2001000600016 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Brazilian Journal of Medical and Biological Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
797-801 |
dc.publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
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UNIFESP |
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Repositório Institucional da UNIFESP |
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