Jaw and Long Bone Marrows Have a Different Osteoclastogenic Potential
Autor(a) principal: | |
---|---|
Data de Publicação: | 2011 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://dx.doi.org/10.1007/s00223-010-9418-4 http://repositorio.unifesp.br/handle/11600/33179 |
Resumo: | Osteoclasts, the multinucleated bone-resorbing cells, arise through fusion of precursors from the myeloid lineage. However, not all osteoclasts are alike; osteoclasts at different bone sites appear to differ in numerous respects. We investigated whether bone marrow cells obtained from jaw and long bone differed in their osteoclastogenic potential. Bone marrow cells from murine mandible and tibiae were isolated and cultured for 4 and 6 days on plastic or 6 and 10 days on dentin. Osteoclastogenesis was assessed by counting the number of TRAP(+) multinucleated cells. Bone marrow cell composition was analyzed by FACS. the expression of osteoclast- and osteoclastogenesis-related genes was studied by qPCR. TRAP activity and resorptive activity of osteoclasts were measured by absorbance and morphometric analyses, respectively. At day 4 more osteoclasts were formed in long bone cultures than in jaw cultures. At day 6 the difference in number was no longer observed. the jaw cultures, however, contained more large osteoclasts on plastic and on dentin. Long bone marrow contained more osteoclast precursors, in particular the myeloid blasts, and qPCR revealed that the RANKL:OPG ratio was higher in long bone cultures. TRAP expression was higher for the long bone cultures on dentin. Although jaw osteoclasts were larger than long bone osteoclasts, no differences were found between their resorptive activities. in conclusion, bone marrow cells from different skeletal locations (jaw and long bone) have different dynamics of osteoclastogenesis. We propose that this is primarily due to differences in the cellular composition of the bone site-specific marrow. |
id |
UFSP_5131614bcb8e716bef2c03e90a557d05 |
---|---|
oai_identifier_str |
oai:repositorio.unifesp.br/:11600/33179 |
network_acronym_str |
UFSP |
network_name_str |
Repositório Institucional da UNIFESP |
repository_id_str |
3465 |
spelling |
Jaw and Long Bone Marrows Have a Different Osteoclastogenic PotentialJawLong boneOsteoclastogenesisOsteoclast precursorHeterogeneityMarrowOsteoclasts, the multinucleated bone-resorbing cells, arise through fusion of precursors from the myeloid lineage. However, not all osteoclasts are alike; osteoclasts at different bone sites appear to differ in numerous respects. We investigated whether bone marrow cells obtained from jaw and long bone differed in their osteoclastogenic potential. Bone marrow cells from murine mandible and tibiae were isolated and cultured for 4 and 6 days on plastic or 6 and 10 days on dentin. Osteoclastogenesis was assessed by counting the number of TRAP(+) multinucleated cells. Bone marrow cell composition was analyzed by FACS. the expression of osteoclast- and osteoclastogenesis-related genes was studied by qPCR. TRAP activity and resorptive activity of osteoclasts were measured by absorbance and morphometric analyses, respectively. At day 4 more osteoclasts were formed in long bone cultures than in jaw cultures. At day 6 the difference in number was no longer observed. the jaw cultures, however, contained more large osteoclasts on plastic and on dentin. Long bone marrow contained more osteoclast precursors, in particular the myeloid blasts, and qPCR revealed that the RANKL:OPG ratio was higher in long bone cultures. TRAP expression was higher for the long bone cultures on dentin. Although jaw osteoclasts were larger than long bone osteoclasts, no differences were found between their resorptive activities. in conclusion, bone marrow cells from different skeletal locations (jaw and long bone) have different dynamics of osteoclastogenesis. We propose that this is primarily due to differences in the cellular composition of the bone site-specific marrow.Univ Amsterdam, Dept Oral Cell Biol & Periodontol, Acad Ctr Dent Amsterdam ACTA, Res Inst Move, NL-1081 LA Amsterdam, NetherlandsVrije Univ Amsterdam, NL-1081 LA Amsterdam, NetherlandsUniversidade Federal de São Paulo UNIFESP, Dept Morphol & Genet, BR-04023900 São Paulo, BrazilUniv Estadual Paulista UNESP, Dept Morphol, Sch Dent, BR-14801903 Araraquara, SP, BrazilUniversidade Federal de São Paulo UNIFESP, Dept Morphol & Genet, BR-04023900 São Paulo, BrazilWeb of ScienceCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)CAPES: BEX:1174/08-8SpringerUniv AmsterdamVrije Univ AmsterdamUniversidade Federal de São Paulo (UNIFESP)Univ Estadual Paulista UNESPFaloni, Ana Paula de Souza [UNIFESP]Schoenmaker, TonAzari, AzinKatchburian, Eduardo [UNIFESP]Cerri, Paulo Sergio [UNIFESP]Vries, Teun J. deEverts, Vincent2016-01-24T14:05:50Z2016-01-24T14:05:50Z2011-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion63-74application/pdfhttp://dx.doi.org/10.1007/s00223-010-9418-4Calcified Tissue International. New York: Springer, v. 88, n. 1, p. 63-74, 2011.10.1007/s00223-010-9418-4WOS000286201200009.pdf0171-967Xhttp://repositorio.unifesp.br/handle/11600/33179WOS:000286201200009engCalcified Tissue Internationalinfo:eu-repo/semantics/openAccesshttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0reponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-08T00:01:34Zoai:repositorio.unifesp.br/:11600/33179Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-08T00:01:34Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
Jaw and Long Bone Marrows Have a Different Osteoclastogenic Potential |
title |
Jaw and Long Bone Marrows Have a Different Osteoclastogenic Potential |
spellingShingle |
Jaw and Long Bone Marrows Have a Different Osteoclastogenic Potential Faloni, Ana Paula de Souza [UNIFESP] Jaw Long bone Osteoclastogenesis Osteoclast precursor Heterogeneity Marrow |
title_short |
Jaw and Long Bone Marrows Have a Different Osteoclastogenic Potential |
title_full |
Jaw and Long Bone Marrows Have a Different Osteoclastogenic Potential |
title_fullStr |
Jaw and Long Bone Marrows Have a Different Osteoclastogenic Potential |
title_full_unstemmed |
Jaw and Long Bone Marrows Have a Different Osteoclastogenic Potential |
title_sort |
Jaw and Long Bone Marrows Have a Different Osteoclastogenic Potential |
author |
Faloni, Ana Paula de Souza [UNIFESP] |
author_facet |
Faloni, Ana Paula de Souza [UNIFESP] Schoenmaker, Ton Azari, Azin Katchburian, Eduardo [UNIFESP] Cerri, Paulo Sergio [UNIFESP] Vries, Teun J. de Everts, Vincent |
author_role |
author |
author2 |
Schoenmaker, Ton Azari, Azin Katchburian, Eduardo [UNIFESP] Cerri, Paulo Sergio [UNIFESP] Vries, Teun J. de Everts, Vincent |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Univ Amsterdam Vrije Univ Amsterdam Universidade Federal de São Paulo (UNIFESP) Univ Estadual Paulista UNESP |
dc.contributor.author.fl_str_mv |
Faloni, Ana Paula de Souza [UNIFESP] Schoenmaker, Ton Azari, Azin Katchburian, Eduardo [UNIFESP] Cerri, Paulo Sergio [UNIFESP] Vries, Teun J. de Everts, Vincent |
dc.subject.por.fl_str_mv |
Jaw Long bone Osteoclastogenesis Osteoclast precursor Heterogeneity Marrow |
topic |
Jaw Long bone Osteoclastogenesis Osteoclast precursor Heterogeneity Marrow |
description |
Osteoclasts, the multinucleated bone-resorbing cells, arise through fusion of precursors from the myeloid lineage. However, not all osteoclasts are alike; osteoclasts at different bone sites appear to differ in numerous respects. We investigated whether bone marrow cells obtained from jaw and long bone differed in their osteoclastogenic potential. Bone marrow cells from murine mandible and tibiae were isolated and cultured for 4 and 6 days on plastic or 6 and 10 days on dentin. Osteoclastogenesis was assessed by counting the number of TRAP(+) multinucleated cells. Bone marrow cell composition was analyzed by FACS. the expression of osteoclast- and osteoclastogenesis-related genes was studied by qPCR. TRAP activity and resorptive activity of osteoclasts were measured by absorbance and morphometric analyses, respectively. At day 4 more osteoclasts were formed in long bone cultures than in jaw cultures. At day 6 the difference in number was no longer observed. the jaw cultures, however, contained more large osteoclasts on plastic and on dentin. Long bone marrow contained more osteoclast precursors, in particular the myeloid blasts, and qPCR revealed that the RANKL:OPG ratio was higher in long bone cultures. TRAP expression was higher for the long bone cultures on dentin. Although jaw osteoclasts were larger than long bone osteoclasts, no differences were found between their resorptive activities. in conclusion, bone marrow cells from different skeletal locations (jaw and long bone) have different dynamics of osteoclastogenesis. We propose that this is primarily due to differences in the cellular composition of the bone site-specific marrow. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-01-01 2016-01-24T14:05:50Z 2016-01-24T14:05:50Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s00223-010-9418-4 Calcified Tissue International. New York: Springer, v. 88, n. 1, p. 63-74, 2011. 10.1007/s00223-010-9418-4 WOS000286201200009.pdf 0171-967X http://repositorio.unifesp.br/handle/11600/33179 WOS:000286201200009 |
url |
http://dx.doi.org/10.1007/s00223-010-9418-4 http://repositorio.unifesp.br/handle/11600/33179 |
identifier_str_mv |
Calcified Tissue International. New York: Springer, v. 88, n. 1, p. 63-74, 2011. 10.1007/s00223-010-9418-4 WOS000286201200009.pdf 0171-967X WOS:000286201200009 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Calcified Tissue International |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess http://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0 |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0 |
dc.format.none.fl_str_mv |
63-74 application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
_version_ |
1814268321126678528 |