Jaw and Long Bone Marrows Have a Different Osteoclastogenic Potential

Detalhes bibliográficos
Autor(a) principal: Faloni, Ana Paula de Souza [UNIFESP]
Data de Publicação: 2011
Outros Autores: Schoenmaker, Ton, Azari, Azin, Katchburian, Eduardo [UNIFESP], Cerri, Paulo Sergio [UNIFESP], Vries, Teun J. de, Everts, Vincent
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1007/s00223-010-9418-4
http://repositorio.unifesp.br/handle/11600/33179
Resumo: Osteoclasts, the multinucleated bone-resorbing cells, arise through fusion of precursors from the myeloid lineage. However, not all osteoclasts are alike; osteoclasts at different bone sites appear to differ in numerous respects. We investigated whether bone marrow cells obtained from jaw and long bone differed in their osteoclastogenic potential. Bone marrow cells from murine mandible and tibiae were isolated and cultured for 4 and 6 days on plastic or 6 and 10 days on dentin. Osteoclastogenesis was assessed by counting the number of TRAP(+) multinucleated cells. Bone marrow cell composition was analyzed by FACS. the expression of osteoclast- and osteoclastogenesis-related genes was studied by qPCR. TRAP activity and resorptive activity of osteoclasts were measured by absorbance and morphometric analyses, respectively. At day 4 more osteoclasts were formed in long bone cultures than in jaw cultures. At day 6 the difference in number was no longer observed. the jaw cultures, however, contained more large osteoclasts on plastic and on dentin. Long bone marrow contained more osteoclast precursors, in particular the myeloid blasts, and qPCR revealed that the RANKL:OPG ratio was higher in long bone cultures. TRAP expression was higher for the long bone cultures on dentin. Although jaw osteoclasts were larger than long bone osteoclasts, no differences were found between their resorptive activities. in conclusion, bone marrow cells from different skeletal locations (jaw and long bone) have different dynamics of osteoclastogenesis. We propose that this is primarily due to differences in the cellular composition of the bone site-specific marrow.
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spelling Jaw and Long Bone Marrows Have a Different Osteoclastogenic PotentialJawLong boneOsteoclastogenesisOsteoclast precursorHeterogeneityMarrowOsteoclasts, the multinucleated bone-resorbing cells, arise through fusion of precursors from the myeloid lineage. However, not all osteoclasts are alike; osteoclasts at different bone sites appear to differ in numerous respects. We investigated whether bone marrow cells obtained from jaw and long bone differed in their osteoclastogenic potential. Bone marrow cells from murine mandible and tibiae were isolated and cultured for 4 and 6 days on plastic or 6 and 10 days on dentin. Osteoclastogenesis was assessed by counting the number of TRAP(+) multinucleated cells. Bone marrow cell composition was analyzed by FACS. the expression of osteoclast- and osteoclastogenesis-related genes was studied by qPCR. TRAP activity and resorptive activity of osteoclasts were measured by absorbance and morphometric analyses, respectively. At day 4 more osteoclasts were formed in long bone cultures than in jaw cultures. At day 6 the difference in number was no longer observed. the jaw cultures, however, contained more large osteoclasts on plastic and on dentin. Long bone marrow contained more osteoclast precursors, in particular the myeloid blasts, and qPCR revealed that the RANKL:OPG ratio was higher in long bone cultures. TRAP expression was higher for the long bone cultures on dentin. Although jaw osteoclasts were larger than long bone osteoclasts, no differences were found between their resorptive activities. in conclusion, bone marrow cells from different skeletal locations (jaw and long bone) have different dynamics of osteoclastogenesis. We propose that this is primarily due to differences in the cellular composition of the bone site-specific marrow.Univ Amsterdam, Dept Oral Cell Biol & Periodontol, Acad Ctr Dent Amsterdam ACTA, Res Inst Move, NL-1081 LA Amsterdam, NetherlandsVrije Univ Amsterdam, NL-1081 LA Amsterdam, NetherlandsUniversidade Federal de São Paulo UNIFESP, Dept Morphol & Genet, BR-04023900 São Paulo, BrazilUniv Estadual Paulista UNESP, Dept Morphol, Sch Dent, BR-14801903 Araraquara, SP, BrazilUniversidade Federal de São Paulo UNIFESP, Dept Morphol & Genet, BR-04023900 São Paulo, BrazilWeb of ScienceCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)CAPES: BEX:1174/08-8SpringerUniv AmsterdamVrije Univ AmsterdamUniversidade Federal de São Paulo (UNIFESP)Univ Estadual Paulista UNESPFaloni, Ana Paula de Souza [UNIFESP]Schoenmaker, TonAzari, AzinKatchburian, Eduardo [UNIFESP]Cerri, Paulo Sergio [UNIFESP]Vries, Teun J. deEverts, Vincent2016-01-24T14:05:50Z2016-01-24T14:05:50Z2011-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion63-74application/pdfhttp://dx.doi.org/10.1007/s00223-010-9418-4Calcified Tissue International. New York: Springer, v. 88, n. 1, p. 63-74, 2011.10.1007/s00223-010-9418-4WOS000286201200009.pdf0171-967Xhttp://repositorio.unifesp.br/handle/11600/33179WOS:000286201200009engCalcified Tissue Internationalinfo:eu-repo/semantics/openAccesshttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0reponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-08T00:01:34Zoai:repositorio.unifesp.br/:11600/33179Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-08T00:01:34Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Jaw and Long Bone Marrows Have a Different Osteoclastogenic Potential
title Jaw and Long Bone Marrows Have a Different Osteoclastogenic Potential
spellingShingle Jaw and Long Bone Marrows Have a Different Osteoclastogenic Potential
Faloni, Ana Paula de Souza [UNIFESP]
Jaw
Long bone
Osteoclastogenesis
Osteoclast precursor
Heterogeneity
Marrow
title_short Jaw and Long Bone Marrows Have a Different Osteoclastogenic Potential
title_full Jaw and Long Bone Marrows Have a Different Osteoclastogenic Potential
title_fullStr Jaw and Long Bone Marrows Have a Different Osteoclastogenic Potential
title_full_unstemmed Jaw and Long Bone Marrows Have a Different Osteoclastogenic Potential
title_sort Jaw and Long Bone Marrows Have a Different Osteoclastogenic Potential
author Faloni, Ana Paula de Souza [UNIFESP]
author_facet Faloni, Ana Paula de Souza [UNIFESP]
Schoenmaker, Ton
Azari, Azin
Katchburian, Eduardo [UNIFESP]
Cerri, Paulo Sergio [UNIFESP]
Vries, Teun J. de
Everts, Vincent
author_role author
author2 Schoenmaker, Ton
Azari, Azin
Katchburian, Eduardo [UNIFESP]
Cerri, Paulo Sergio [UNIFESP]
Vries, Teun J. de
Everts, Vincent
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Univ Amsterdam
Vrije Univ Amsterdam
Universidade Federal de São Paulo (UNIFESP)
Univ Estadual Paulista UNESP
dc.contributor.author.fl_str_mv Faloni, Ana Paula de Souza [UNIFESP]
Schoenmaker, Ton
Azari, Azin
Katchburian, Eduardo [UNIFESP]
Cerri, Paulo Sergio [UNIFESP]
Vries, Teun J. de
Everts, Vincent
dc.subject.por.fl_str_mv Jaw
Long bone
Osteoclastogenesis
Osteoclast precursor
Heterogeneity
Marrow
topic Jaw
Long bone
Osteoclastogenesis
Osteoclast precursor
Heterogeneity
Marrow
description Osteoclasts, the multinucleated bone-resorbing cells, arise through fusion of precursors from the myeloid lineage. However, not all osteoclasts are alike; osteoclasts at different bone sites appear to differ in numerous respects. We investigated whether bone marrow cells obtained from jaw and long bone differed in their osteoclastogenic potential. Bone marrow cells from murine mandible and tibiae were isolated and cultured for 4 and 6 days on plastic or 6 and 10 days on dentin. Osteoclastogenesis was assessed by counting the number of TRAP(+) multinucleated cells. Bone marrow cell composition was analyzed by FACS. the expression of osteoclast- and osteoclastogenesis-related genes was studied by qPCR. TRAP activity and resorptive activity of osteoclasts were measured by absorbance and morphometric analyses, respectively. At day 4 more osteoclasts were formed in long bone cultures than in jaw cultures. At day 6 the difference in number was no longer observed. the jaw cultures, however, contained more large osteoclasts on plastic and on dentin. Long bone marrow contained more osteoclast precursors, in particular the myeloid blasts, and qPCR revealed that the RANKL:OPG ratio was higher in long bone cultures. TRAP expression was higher for the long bone cultures on dentin. Although jaw osteoclasts were larger than long bone osteoclasts, no differences were found between their resorptive activities. in conclusion, bone marrow cells from different skeletal locations (jaw and long bone) have different dynamics of osteoclastogenesis. We propose that this is primarily due to differences in the cellular composition of the bone site-specific marrow.
publishDate 2011
dc.date.none.fl_str_mv 2011-01-01
2016-01-24T14:05:50Z
2016-01-24T14:05:50Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s00223-010-9418-4
Calcified Tissue International. New York: Springer, v. 88, n. 1, p. 63-74, 2011.
10.1007/s00223-010-9418-4
WOS000286201200009.pdf
0171-967X
http://repositorio.unifesp.br/handle/11600/33179
WOS:000286201200009
url http://dx.doi.org/10.1007/s00223-010-9418-4
http://repositorio.unifesp.br/handle/11600/33179
identifier_str_mv Calcified Tissue International. New York: Springer, v. 88, n. 1, p. 63-74, 2011.
10.1007/s00223-010-9418-4
WOS000286201200009.pdf
0171-967X
WOS:000286201200009
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Calcified Tissue International
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
http://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
eu_rights_str_mv openAccess
rights_invalid_str_mv http://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.format.none.fl_str_mv 63-74
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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