Chromosome localization changes in the Trypanosoma cruzi nucleus
Autor(a) principal: | |
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Data de Publicação: | 2002 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/27048 http://dx.doi.org/10.1128/EC.1.6.944-953.2002 |
Resumo: | Chromosome localization in the interphase nuclei of eukaryotes depends on gene replication and transcription. Little is known about chromosome localization in protozoan parasites such as trypanosomes, which have unique mechanisms for the control of gene expression, with most genes being posttranscriptionally regulated. in the present study, we examined where the chromosomes are replicated in Trypanosoma cruzi, the agent of Chagas' disease. the replication sites, identified by the incorporation of 5-bromodeoxyuridine, are located at the nuclear periphery in proliferating epimastigote forms in the early S phase of the cell cycle. When the S phase ends and cells progress through the cell cycle, 5-bromodeoxyuridine labeling is observed in the nuclear interior, suggesting that chromosomes move. We next monitored chromosome locations in different stages of the cell cycle by using a satellite DNA sequence as a probe in a fluorescence in situ hybridization assay. We found two distinct labeling patterns according to the cell cycle stage. the first one is seen in the G, phase, in hydroxyurea-arrested epimastigotes or in trypomastigotes, which are differentiated nondividing forms. in all of these forms the satellite DNA is found in dots randomly dispersed in the nucleus. the other pattern is found in cells from the S phase to the G, phase. in these cells, the satellite DNA is found preferentially at the nuclear periphery. the labeling at the nuclear periphery disappears only after mitosis. Also, DNA detected with terminal deoxynucleotidyl transferase is found distributed throughout the nuclear space in the G, phase but concentrated at the nuclear periphery in the S phase to the G, phase. These results strongly suggest that T. cruzi chromosomes move and, after entering the S phase, become constrained at the nuclear periphery, where replication occurs. |
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Carolina, M.Elias, Q. B.Faria, M.Mortara, R. A.Motta, MCMSouza, W. deThiry, M.Schenkman, S. [UNIFESP]Universidade Federal de São Paulo (UNIFESP)Universidade Federal do Rio de Janeiro (UFRJ)Univ Liege2016-01-24T12:33:36Z2016-01-24T12:33:36Z2002-12-01Eukaryotic Cell. Washington: Amer Soc Microbiology, v. 1, n. 6, p. 944-953, 2002.1535-9778http://repositorio.unifesp.br/handle/11600/27048http://dx.doi.org/10.1128/EC.1.6.944-953.2002WOS000179722200011.pdf10.1128/EC.1.6.944-953.2002WOS:000179722200011Chromosome localization in the interphase nuclei of eukaryotes depends on gene replication and transcription. Little is known about chromosome localization in protozoan parasites such as trypanosomes, which have unique mechanisms for the control of gene expression, with most genes being posttranscriptionally regulated. in the present study, we examined where the chromosomes are replicated in Trypanosoma cruzi, the agent of Chagas' disease. the replication sites, identified by the incorporation of 5-bromodeoxyuridine, are located at the nuclear periphery in proliferating epimastigote forms in the early S phase of the cell cycle. When the S phase ends and cells progress through the cell cycle, 5-bromodeoxyuridine labeling is observed in the nuclear interior, suggesting that chromosomes move. We next monitored chromosome locations in different stages of the cell cycle by using a satellite DNA sequence as a probe in a fluorescence in situ hybridization assay. We found two distinct labeling patterns according to the cell cycle stage. the first one is seen in the G, phase, in hydroxyurea-arrested epimastigotes or in trypomastigotes, which are differentiated nondividing forms. in all of these forms the satellite DNA is found in dots randomly dispersed in the nucleus. the other pattern is found in cells from the S phase to the G, phase. in these cells, the satellite DNA is found preferentially at the nuclear periphery. the labeling at the nuclear periphery disappears only after mitosis. Also, DNA detected with terminal deoxynucleotidyl transferase is found distributed throughout the nuclear space in the G, phase but concentrated at the nuclear periphery in the S phase to the G, phase. These results strongly suggest that T. cruzi chromosomes move and, after entering the S phase, become constrained at the nuclear periphery, where replication occurs.Universidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, BR-04023062 São Paulo, BrazilUniv Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, Lab Ultraestrutura Celular Hertha Meyer, BR-21941 Rio de Janeiro, BrazilUniv Liege, Lab Cellular & Tissue Biol, Liege, BelgiumUniversidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, BR-04023062 São Paulo, BrazilWeb of Science944-953engAmer Soc MicrobiologyEukaryotic CellChromosome localization changes in the Trypanosoma cruzi nucleusinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000179722200011.pdfapplication/pdf1730448${dspace.ui.url}/bitstream/11600/27048/1/WOS000179722200011.pdf857e082093950a8fd5371ada42b77d64MD51open accessTEXTWOS000179722200011.pdf.txtWOS000179722200011.pdf.txtExtracted texttext/plain50167${dspace.ui.url}/bitstream/11600/27048/2/WOS000179722200011.pdf.txt782099f18b99782f74e0c634d7c43ff3MD52open access11600/270482023-01-12 21:52:30.183open accessoai:repositorio.unifesp.br:11600/27048Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-01-13T00:52:30Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Chromosome localization changes in the Trypanosoma cruzi nucleus |
title |
Chromosome localization changes in the Trypanosoma cruzi nucleus |
spellingShingle |
Chromosome localization changes in the Trypanosoma cruzi nucleus Carolina, M. |
title_short |
Chromosome localization changes in the Trypanosoma cruzi nucleus |
title_full |
Chromosome localization changes in the Trypanosoma cruzi nucleus |
title_fullStr |
Chromosome localization changes in the Trypanosoma cruzi nucleus |
title_full_unstemmed |
Chromosome localization changes in the Trypanosoma cruzi nucleus |
title_sort |
Chromosome localization changes in the Trypanosoma cruzi nucleus |
author |
Carolina, M. |
author_facet |
Carolina, M. Elias, Q. B. Faria, M. Mortara, R. A. Motta, MCM Souza, W. de Thiry, M. Schenkman, S. [UNIFESP] |
author_role |
author |
author2 |
Elias, Q. B. Faria, M. Mortara, R. A. Motta, MCM Souza, W. de Thiry, M. Schenkman, S. [UNIFESP] |
author2_role |
author author author author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Universidade Federal do Rio de Janeiro (UFRJ) Univ Liege |
dc.contributor.author.fl_str_mv |
Carolina, M. Elias, Q. B. Faria, M. Mortara, R. A. Motta, MCM Souza, W. de Thiry, M. Schenkman, S. [UNIFESP] |
description |
Chromosome localization in the interphase nuclei of eukaryotes depends on gene replication and transcription. Little is known about chromosome localization in protozoan parasites such as trypanosomes, which have unique mechanisms for the control of gene expression, with most genes being posttranscriptionally regulated. in the present study, we examined where the chromosomes are replicated in Trypanosoma cruzi, the agent of Chagas' disease. the replication sites, identified by the incorporation of 5-bromodeoxyuridine, are located at the nuclear periphery in proliferating epimastigote forms in the early S phase of the cell cycle. When the S phase ends and cells progress through the cell cycle, 5-bromodeoxyuridine labeling is observed in the nuclear interior, suggesting that chromosomes move. We next monitored chromosome locations in different stages of the cell cycle by using a satellite DNA sequence as a probe in a fluorescence in situ hybridization assay. We found two distinct labeling patterns according to the cell cycle stage. the first one is seen in the G, phase, in hydroxyurea-arrested epimastigotes or in trypomastigotes, which are differentiated nondividing forms. in all of these forms the satellite DNA is found in dots randomly dispersed in the nucleus. the other pattern is found in cells from the S phase to the G, phase. in these cells, the satellite DNA is found preferentially at the nuclear periphery. the labeling at the nuclear periphery disappears only after mitosis. Also, DNA detected with terminal deoxynucleotidyl transferase is found distributed throughout the nuclear space in the G, phase but concentrated at the nuclear periphery in the S phase to the G, phase. These results strongly suggest that T. cruzi chromosomes move and, after entering the S phase, become constrained at the nuclear periphery, where replication occurs. |
publishDate |
2002 |
dc.date.issued.fl_str_mv |
2002-12-01 |
dc.date.accessioned.fl_str_mv |
2016-01-24T12:33:36Z |
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2016-01-24T12:33:36Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Eukaryotic Cell. Washington: Amer Soc Microbiology, v. 1, n. 6, p. 944-953, 2002. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/27048 http://dx.doi.org/10.1128/EC.1.6.944-953.2002 |
dc.identifier.issn.none.fl_str_mv |
1535-9778 |
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WOS000179722200011.pdf |
dc.identifier.doi.none.fl_str_mv |
10.1128/EC.1.6.944-953.2002 |
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WOS:000179722200011 |
identifier_str_mv |
Eukaryotic Cell. Washington: Amer Soc Microbiology, v. 1, n. 6, p. 944-953, 2002. 1535-9778 WOS000179722200011.pdf 10.1128/EC.1.6.944-953.2002 WOS:000179722200011 |
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http://repositorio.unifesp.br/handle/11600/27048 http://dx.doi.org/10.1128/EC.1.6.944-953.2002 |
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Amer Soc Microbiology |
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Amer Soc Microbiology |
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