Vitamin B-12-induced spermatogenesis recovery in cimetidine-treated rats: effect on the spermatogonia number and sperm concentration

Detalhes bibliográficos
Autor(a) principal: Beltrame, Flavia L. [UNIFESP]
Data de Publicação: 2017
Outros Autores: Sasso-Cerri, Estela
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.4103/1008-682X.182397
http://repositorio.unifesp.br/handle/11600/51353
Resumo: The H-2-receptor antagonist cimetidine is an antiulcer drug also used for the treatment of cancer due to its antiangiogenic effect. However, this drug has caused structural changes in the seminiferous tubules. Vitamin B-12 has been used as a therapeutic agent for the treatment of male infertility. The supplementation of rats with vitamin B-12 during cimetidine treatment has recovered the damaged seminiferous tubules, but how this vitamin restores the seminiferous epithelium has not been clarified. In this study, we evaluated whether vitamin B-12 improves the number of spermatogonia, spermatocytes, and sperm concentration in cimetidine-treated rats. Adult male rats were treated for 50 days as follows: cimetidine group received 100 mg kg(-1) b.w. of cimetidine, cimetidine-B-12 group received cimetidine and 3 mu g of vitamin B-12-hydroxocobalamin, B-12 group received only 3 mu g of vitamin, and control group received saline. Sperm concentration was calculated and historesin-embedded testes sections were used for the quantitative analyses of spermatogonia (A
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spelling Vitamin B-12-induced spermatogenesis recovery in cimetidine-treated rats: effect on the spermatogonia number and sperm concentrationcimetidinefertilityspermatogenesistestisvitamin B-12The H-2-receptor antagonist cimetidine is an antiulcer drug also used for the treatment of cancer due to its antiangiogenic effect. However, this drug has caused structural changes in the seminiferous tubules. Vitamin B-12 has been used as a therapeutic agent for the treatment of male infertility. The supplementation of rats with vitamin B-12 during cimetidine treatment has recovered the damaged seminiferous tubules, but how this vitamin restores the seminiferous epithelium has not been clarified. In this study, we evaluated whether vitamin B-12 improves the number of spermatogonia, spermatocytes, and sperm concentration in cimetidine-treated rats. Adult male rats were treated for 50 days as follows: cimetidine group received 100 mg kg(-1) b.w. of cimetidine, cimetidine-B-12 group received cimetidine and 3 mu g of vitamin B-12-hydroxocobalamin, B-12 group received only 3 mu g of vitamin, and control group received saline. Sperm concentration was calculated and historesin-embedded testes sections were used for the quantitative analyses of spermatogonia (AIn/B) and spermatocytes. TUNEL method and PCNA immunofluorescence were performed. Cimetidine caused a significant reduction in sperm concentration. TUNEL-positive spermatogonia and spermatocytes were correlated to a significant reduction in the number of these cells. In cimetidine-B-12 group, sperm concentration was higher than cimetidine group and a significant increase in the number of spermatogonia (stages II-VI) was correlated to a high incidence of PCNA-immunolabeled spermatogonia and spermatocytes. The results show that the supplementation of rats with vitamin B-12 during cimetidine treatment increases sperm concentration and exerts a potential effect in the recovery of spermatogonia and spermatocytes.Fed Univ São Paulo UNIFESP EPM, Dept Morphol & Genet, São Paulo, BrazilSão Paulo State Univ UNESP FOAr, Sch Dent, Lab Histol & Embryol, Dept Morphol, São Paulo, BrazilFed Univ São Paulo UNIFESP EPM, Dept Morphol & Genet, São Paulo, BrazilWeb of ScienceFAPESP [2006/54776-6, 2010/02409-5, 2012/23845-3, 2013/25322-0]CNPqMedknow Publications & Media Pvt Ltd2019-08-19T11:49:36Z2019-08-19T11:49:36Z2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion567-572application/pdfhttp://dx.doi.org/10.4103/1008-682X.182397Asian Journal Of Andrology. Mumbai, v. 19, n. 5, p. 567-572, 2017.10.4103/1008-682X.182397WOS000407906900012.pdf1008-682Xhttp://repositorio.unifesp.br/handle/11600/51353WOS:000407906900012enginfo:eu-repo/semantics/openAccessBeltrame, Flavia L. [UNIFESP]Sasso-Cerri, Estelareponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-03T01:24:56Zoai:repositorio.unifesp.br/:11600/51353Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-03T01:24:56Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Vitamin B-12-induced spermatogenesis recovery in cimetidine-treated rats: effect on the spermatogonia number and sperm concentration
title Vitamin B-12-induced spermatogenesis recovery in cimetidine-treated rats: effect on the spermatogonia number and sperm concentration
spellingShingle Vitamin B-12-induced spermatogenesis recovery in cimetidine-treated rats: effect on the spermatogonia number and sperm concentration
Beltrame, Flavia L. [UNIFESP]
cimetidine
fertility
spermatogenesis
testis
vitamin B-12
title_short Vitamin B-12-induced spermatogenesis recovery in cimetidine-treated rats: effect on the spermatogonia number and sperm concentration
title_full Vitamin B-12-induced spermatogenesis recovery in cimetidine-treated rats: effect on the spermatogonia number and sperm concentration
title_fullStr Vitamin B-12-induced spermatogenesis recovery in cimetidine-treated rats: effect on the spermatogonia number and sperm concentration
title_full_unstemmed Vitamin B-12-induced spermatogenesis recovery in cimetidine-treated rats: effect on the spermatogonia number and sperm concentration
title_sort Vitamin B-12-induced spermatogenesis recovery in cimetidine-treated rats: effect on the spermatogonia number and sperm concentration
author Beltrame, Flavia L. [UNIFESP]
author_facet Beltrame, Flavia L. [UNIFESP]
Sasso-Cerri, Estela
author_role author
author2 Sasso-Cerri, Estela
author2_role author
dc.contributor.author.fl_str_mv Beltrame, Flavia L. [UNIFESP]
Sasso-Cerri, Estela
dc.subject.por.fl_str_mv cimetidine
fertility
spermatogenesis
testis
vitamin B-12
topic cimetidine
fertility
spermatogenesis
testis
vitamin B-12
description The H-2-receptor antagonist cimetidine is an antiulcer drug also used for the treatment of cancer due to its antiangiogenic effect. However, this drug has caused structural changes in the seminiferous tubules. Vitamin B-12 has been used as a therapeutic agent for the treatment of male infertility. The supplementation of rats with vitamin B-12 during cimetidine treatment has recovered the damaged seminiferous tubules, but how this vitamin restores the seminiferous epithelium has not been clarified. In this study, we evaluated whether vitamin B-12 improves the number of spermatogonia, spermatocytes, and sperm concentration in cimetidine-treated rats. Adult male rats were treated for 50 days as follows: cimetidine group received 100 mg kg(-1) b.w. of cimetidine, cimetidine-B-12 group received cimetidine and 3 mu g of vitamin B-12-hydroxocobalamin, B-12 group received only 3 mu g of vitamin, and control group received saline. Sperm concentration was calculated and historesin-embedded testes sections were used for the quantitative analyses of spermatogonia (A
publishDate 2017
dc.date.none.fl_str_mv 2017
2019-08-19T11:49:36Z
2019-08-19T11:49:36Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.4103/1008-682X.182397
Asian Journal Of Andrology. Mumbai, v. 19, n. 5, p. 567-572, 2017.
10.4103/1008-682X.182397
WOS000407906900012.pdf
1008-682X
http://repositorio.unifesp.br/handle/11600/51353
WOS:000407906900012
url http://dx.doi.org/10.4103/1008-682X.182397
http://repositorio.unifesp.br/handle/11600/51353
identifier_str_mv Asian Journal Of Andrology. Mumbai, v. 19, n. 5, p. 567-572, 2017.
10.4103/1008-682X.182397
WOS000407906900012.pdf
1008-682X
WOS:000407906900012
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 567-572
application/pdf
dc.publisher.none.fl_str_mv Medknow Publications & Media Pvt Ltd
publisher.none.fl_str_mv Medknow Publications & Media Pvt Ltd
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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