Inhibitory effect of the Pseudobrickellia brasiliensis (Spreng) RM King & H. Rob. aqueous extract on human lymphocyte proliferation and IFN-gamma and TNF-alpha production in vitro

Detalhes bibliográficos
Autor(a) principal: Almeida, V. G.
Data de Publicação: 2017
Outros Autores: Avelar-Freitas, B. A., Santos, M. G., Costa, L. A., Silva, T. J., Pereira, W. F., Amorim, M. L. L., Grael, C. F. F., Gregorio, Luiz Elídio [UNIFESP], Rocha-Vieira, E., Brito-Melo, G. E. A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1590/1414-431X20175163
https://repositorio.unifesp.br/handle/11600/56256
Resumo: Pseudobrickellia brasiliensis (Asteraceae) is a plant commonly known as arnica-do-campo and belongs to the native flora of the Brazilian Cerrado. The alcoholic extract of the plant has been used as an anti-inflammatory agent in folk medicine, but the biological mechanism of action has not been elucidated. The present study evaluated the composition of P. brasiliensis aqueous extract and its effects on pro-inflammatory cytokine production and lymphocyte proliferation. The extracts were prepared by sequential maceration of P. brasiliensis leaves in ethanol, ethyl acetate, and water. Extract cytotoxicity was evaluated by trypan blue exclusion assay, and apoptosis and necrosis were measured by staining with annexin V-FITC and propidium iodide. The ethanolic (ETA) and acetate (ACE) extracts showed cytotoxic effects. The aqueous extract (AQU) was not cytotoxic. Peripheral blood mononuclear cells stimulated with phorbol myristate acetate and ionomycin and treated with AQU (100 mu g/mL) showed reduced interferon (IFN)-gamma and tumor necrosis factor (TNF)-alpha expression. AQU also inhibited lymphocyte proliferative response after nonspecific stimulation with phytohemagglutinin. The aqueous extract was analyzed by liquid chromatography coupled with photodiode array detection and mass spectrometry. Quinic acid and its derivatives 5-caffeoylquinic acid and 3,5dicaffeoylquinic acid, as well as the flavonoids luteolin and luteolin dihexoside, were detected. All these compounds are known to exhibit anti-inflammatory activity. Taken together, these findings demonstrate that P. brasiliensis aqueous extract can inhibit the pro-inflammatory cytokine production and proliferative response of lymphocytes. These effects may be related to the presence of chemical substances with anti-inflammatory actions previously reported in scientific literature.
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spelling Inhibitory effect of the Pseudobrickellia brasiliensis (Spreng) RM King & H. Rob. aqueous extract on human lymphocyte proliferation and IFN-gamma and TNF-alpha production in vitroCytokinesLymphocyte proliferationLuteolinMedicinal plantPseudobrickellia brasiliensisQuinic acidPseudobrickellia brasiliensis (Asteraceae) is a plant commonly known as arnica-do-campo and belongs to the native flora of the Brazilian Cerrado. The alcoholic extract of the plant has been used as an anti-inflammatory agent in folk medicine, but the biological mechanism of action has not been elucidated. The present study evaluated the composition of P. brasiliensis aqueous extract and its effects on pro-inflammatory cytokine production and lymphocyte proliferation. The extracts were prepared by sequential maceration of P. brasiliensis leaves in ethanol, ethyl acetate, and water. Extract cytotoxicity was evaluated by trypan blue exclusion assay, and apoptosis and necrosis were measured by staining with annexin V-FITC and propidium iodide. The ethanolic (ETA) and acetate (ACE) extracts showed cytotoxic effects. The aqueous extract (AQU) was not cytotoxic. Peripheral blood mononuclear cells stimulated with phorbol myristate acetate and ionomycin and treated with AQU (100 mu g/mL) showed reduced interferon (IFN)-gamma and tumor necrosis factor (TNF)-alpha expression. AQU also inhibited lymphocyte proliferative response after nonspecific stimulation with phytohemagglutinin. The aqueous extract was analyzed by liquid chromatography coupled with photodiode array detection and mass spectrometry. Quinic acid and its derivatives 5-caffeoylquinic acid and 3,5dicaffeoylquinic acid, as well as the flavonoids luteolin and luteolin dihexoside, were detected. All these compounds are known to exhibit anti-inflammatory activity. Taken together, these findings demonstrate that P. brasiliensis aqueous extract can inhibit the pro-inflammatory cytokine production and proliferative response of lymphocytes. These effects may be related to the presence of chemical substances with anti-inflammatory actions previously reported in scientific literature.Univ Fed Vales Jequitinhonha & Mucuri, Programa Multictr Posgrad Ciencias Fisiol, Diamantina, MG, BrazilUniv Fed Vales Jequitinhonha & Mucuri, Ctr Integrado Posgrad & Pesquisa Saude Vale Jequi, Lab Imunol, Diamantina, MG, BrazilUniv Fed Vales Jequitinhonha & Mucuri, Dept Farm, Lab Farmacognosia, Diamantina, MG, BrazilUniv Fed Sao Paulo, Dept Ciencias Farmaceut, Lab Insumos Nat & Sintet, Diadema, SP, BrazilLaboratório de Insumos Naturais e Sintéticos, Departamento de Ciências Farmacêuticas, Universidade Federal de São Paulo, Diadema, SP, BrasilWeb of ScienceFundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Assoc Bras Divulg Cientifica2020-07-31T12:46:29Z2020-07-31T12:46:29Z2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion-application/pdfhttp://dx.doi.org/10.1590/1414-431X20175163Brazilian Journal Of Medical And Biological Research. Sao Paulo, v. 50, n. 8, p. -, 2017.10.1590/1414-431X20175163S0100-879X2017000800603.pdf0100-879XS0100-879X2017000800603https://repositorio.unifesp.br/handle/11600/56256WOS:000405405900001engBrazilian Journal Of Medical And Biological ResearchSao Pauloinfo:eu-repo/semantics/openAccessAlmeida, V. G.Avelar-Freitas, B. A.Santos, M. G.Costa, L. A.Silva, T. J.Pereira, W. F.Amorim, M. L. L.Grael, C. F. F.Gregorio, Luiz Elídio [UNIFESP]Rocha-Vieira, E.Brito-Melo, G. E. A.reponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-10T07:50:58Zoai:repositorio.unifesp.br/:11600/56256Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-10T07:50:58Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Inhibitory effect of the Pseudobrickellia brasiliensis (Spreng) RM King & H. Rob. aqueous extract on human lymphocyte proliferation and IFN-gamma and TNF-alpha production in vitro
title Inhibitory effect of the Pseudobrickellia brasiliensis (Spreng) RM King & H. Rob. aqueous extract on human lymphocyte proliferation and IFN-gamma and TNF-alpha production in vitro
spellingShingle Inhibitory effect of the Pseudobrickellia brasiliensis (Spreng) RM King & H. Rob. aqueous extract on human lymphocyte proliferation and IFN-gamma and TNF-alpha production in vitro
Almeida, V. G.
Cytokines
Lymphocyte proliferation
Luteolin
Medicinal plant
Pseudobrickellia brasiliensis
Quinic acid
title_short Inhibitory effect of the Pseudobrickellia brasiliensis (Spreng) RM King & H. Rob. aqueous extract on human lymphocyte proliferation and IFN-gamma and TNF-alpha production in vitro
title_full Inhibitory effect of the Pseudobrickellia brasiliensis (Spreng) RM King & H. Rob. aqueous extract on human lymphocyte proliferation and IFN-gamma and TNF-alpha production in vitro
title_fullStr Inhibitory effect of the Pseudobrickellia brasiliensis (Spreng) RM King & H. Rob. aqueous extract on human lymphocyte proliferation and IFN-gamma and TNF-alpha production in vitro
title_full_unstemmed Inhibitory effect of the Pseudobrickellia brasiliensis (Spreng) RM King & H. Rob. aqueous extract on human lymphocyte proliferation and IFN-gamma and TNF-alpha production in vitro
title_sort Inhibitory effect of the Pseudobrickellia brasiliensis (Spreng) RM King & H. Rob. aqueous extract on human lymphocyte proliferation and IFN-gamma and TNF-alpha production in vitro
author Almeida, V. G.
author_facet Almeida, V. G.
Avelar-Freitas, B. A.
Santos, M. G.
Costa, L. A.
Silva, T. J.
Pereira, W. F.
Amorim, M. L. L.
Grael, C. F. F.
Gregorio, Luiz Elídio [UNIFESP]
Rocha-Vieira, E.
Brito-Melo, G. E. A.
author_role author
author2 Avelar-Freitas, B. A.
Santos, M. G.
Costa, L. A.
Silva, T. J.
Pereira, W. F.
Amorim, M. L. L.
Grael, C. F. F.
Gregorio, Luiz Elídio [UNIFESP]
Rocha-Vieira, E.
Brito-Melo, G. E. A.
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Almeida, V. G.
Avelar-Freitas, B. A.
Santos, M. G.
Costa, L. A.
Silva, T. J.
Pereira, W. F.
Amorim, M. L. L.
Grael, C. F. F.
Gregorio, Luiz Elídio [UNIFESP]
Rocha-Vieira, E.
Brito-Melo, G. E. A.
dc.subject.por.fl_str_mv Cytokines
Lymphocyte proliferation
Luteolin
Medicinal plant
Pseudobrickellia brasiliensis
Quinic acid
topic Cytokines
Lymphocyte proliferation
Luteolin
Medicinal plant
Pseudobrickellia brasiliensis
Quinic acid
description Pseudobrickellia brasiliensis (Asteraceae) is a plant commonly known as arnica-do-campo and belongs to the native flora of the Brazilian Cerrado. The alcoholic extract of the plant has been used as an anti-inflammatory agent in folk medicine, but the biological mechanism of action has not been elucidated. The present study evaluated the composition of P. brasiliensis aqueous extract and its effects on pro-inflammatory cytokine production and lymphocyte proliferation. The extracts were prepared by sequential maceration of P. brasiliensis leaves in ethanol, ethyl acetate, and water. Extract cytotoxicity was evaluated by trypan blue exclusion assay, and apoptosis and necrosis were measured by staining with annexin V-FITC and propidium iodide. The ethanolic (ETA) and acetate (ACE) extracts showed cytotoxic effects. The aqueous extract (AQU) was not cytotoxic. Peripheral blood mononuclear cells stimulated with phorbol myristate acetate and ionomycin and treated with AQU (100 mu g/mL) showed reduced interferon (IFN)-gamma and tumor necrosis factor (TNF)-alpha expression. AQU also inhibited lymphocyte proliferative response after nonspecific stimulation with phytohemagglutinin. The aqueous extract was analyzed by liquid chromatography coupled with photodiode array detection and mass spectrometry. Quinic acid and its derivatives 5-caffeoylquinic acid and 3,5dicaffeoylquinic acid, as well as the flavonoids luteolin and luteolin dihexoside, were detected. All these compounds are known to exhibit anti-inflammatory activity. Taken together, these findings demonstrate that P. brasiliensis aqueous extract can inhibit the pro-inflammatory cytokine production and proliferative response of lymphocytes. These effects may be related to the presence of chemical substances with anti-inflammatory actions previously reported in scientific literature.
publishDate 2017
dc.date.none.fl_str_mv 2017
2020-07-31T12:46:29Z
2020-07-31T12:46:29Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/1414-431X20175163
Brazilian Journal Of Medical And Biological Research. Sao Paulo, v. 50, n. 8, p. -, 2017.
10.1590/1414-431X20175163
S0100-879X2017000800603.pdf
0100-879X
S0100-879X2017000800603
https://repositorio.unifesp.br/handle/11600/56256
WOS:000405405900001
url http://dx.doi.org/10.1590/1414-431X20175163
https://repositorio.unifesp.br/handle/11600/56256
identifier_str_mv Brazilian Journal Of Medical And Biological Research. Sao Paulo, v. 50, n. 8, p. -, 2017.
10.1590/1414-431X20175163
S0100-879X2017000800603.pdf
0100-879X
S0100-879X2017000800603
WOS:000405405900001
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Brazilian Journal Of Medical And Biological Research
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv -
application/pdf
dc.coverage.none.fl_str_mv Sao Paulo
dc.publisher.none.fl_str_mv Assoc Bras Divulg Cientifica
publisher.none.fl_str_mv Assoc Bras Divulg Cientifica
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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