Retinyl palmitate polymeric nanocapsules as carriers of bioactives

• Autor(a) principal: Teixeira, Zaine
• Data de Publicação: 2012
• Outros Autores: Dreiss, Cecile A., Lawrence, M. Jayne, Heenan, Richard K., Machado, Daisy, Justo, Giselle Zenker [UNIFESP], Guterres, Silvia Stanisçuaski., Duran, Nelson
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNIFESP
• dARK ID: ark:/48912/001300000ss96
• DOI: 10.1016/j.jcis.2012.05.042
• Texto Completo: http://dx.doi.org/10.1016/j.jcis.2012.05.042; http://repositorio.unifesp.br/handle/11600/35273
• topic: Polymeric nanoparticles; Poly(D,L-lactide); Nanocapsules; Skin permeation; Phototoxicity; Cytotoxicity; Small-angle neutron scattering

Nanocapsules containing poly(D,L-lactide) shell and retinyl palmitate core have been prepared by the preformed polymer interfacial deposition method. Dynamic light scattering measurements yielded an average hydrodynamic diameter of similar to 220 nm and a polydispersity index of similar to 0.12. Small-angle neutron scattering experiments revealed the presence of two populations of nanocapsules of core diameters similar to 192 and 65 nm. Freeze fracture transmission electron microscopy showed a polydisperse population of nanocapsules (NC), with a poly(D,L-lactide) shell thickness between 11 and 3 nm. for comparison purposes, nanoemulsions (NE, no polymer) and nanospheres (NS, polymer matrix) were also prepared. Each type of nanoparticles exhibited a different morphology (when examined by electron microscopy), in particular NC showed deformability by capillary adhesion. All three types of nanoparticles successfully encapsulated the poorly water-soluble molecules baicalein and benzophenone-3. the thermal behavior of the various nanoparticles was different to a physical mixture of its individual components. Cytotoxicity and phototoxicity assays, performed in human keratinocytes (HaCaT) and murine fibroblasts (BALB/c3T3), showed that the NC were only cytotoxic at high concentrations. in vitro release studies of benzophenone-3, by the dialysis bag method using NC and NS, showed a sustained release; however, permeation studies using plastic surgery human abdominal skin in Franz diffusion cells showed that a higher amount of benzophenone-3 from NC penetrated into the skin, most probably due to the deformable nature of these nanoparticles. (C) 2012 Elsevier Inc. All rights reserved.