The Genome Sequence of Leishmania (Leishmania) amazonensis: Functional Annotation and Extended Analysis of Gene Models
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://dx.doi.org/10.1093/dnares/dst031 http://repositorio.unifesp.br/handle/11600/37004 |
Resumo: | We present the sequencing and annotation of the Leishmania (Leishmania) amazonensis genome, an etiological agent of human cutaneous leishmaniasis in the Amazon region of Brazil. L. (L.) amazonensis shares features with Leishmania (L.) mexicana but also exhibits unique characteristics regarding geographical distribution and clinical manifestations of cutaneous lesions (e.g. borderline disseminated cutaneous leishmaniasis). Predicted genes were scored for orthologous gene families and conserved domains in comparison with other human pathogenic Leishmania spp. Carboxypeptidase, aminotransferase, and 3'-nucleotidase genes and ATPase, thioredoxin, and chaperone-related domains were represented more abundantly in L. (L.) amazonensis and L. (L.) mexicana species. Phylogenetic analysis revealed that these two species share groups of amastin surface proteins unique to the genus that could be related to specific features of disease outcomes and host cell interactions. Additionally, we describe a hypothetical hybrid interactome of potentially secreted L. (L.) amazonensis proteins and host proteins under the assumption that parasite factors mimic their mammalian counterparts. the model predicts an interaction between an L. (L.) amazonensis heat-shock protein and mammalian Toll-like receptor 9, which is implicated in important immune responses such as cytokine and nitric oxide production. the analysis presented here represents valuable information for future studies of leishmaniasis pathogenicity and treatment. |
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The Genome Sequence of Leishmania (Leishmania) amazonensis: Functional Annotation and Extended Analysis of Gene ModelsgenomeLeishmania amazonensisinteractomeamastinheat-shock proteinWe present the sequencing and annotation of the Leishmania (Leishmania) amazonensis genome, an etiological agent of human cutaneous leishmaniasis in the Amazon region of Brazil. L. (L.) amazonensis shares features with Leishmania (L.) mexicana but also exhibits unique characteristics regarding geographical distribution and clinical manifestations of cutaneous lesions (e.g. borderline disseminated cutaneous leishmaniasis). Predicted genes were scored for orthologous gene families and conserved domains in comparison with other human pathogenic Leishmania spp. Carboxypeptidase, aminotransferase, and 3'-nucleotidase genes and ATPase, thioredoxin, and chaperone-related domains were represented more abundantly in L. (L.) amazonensis and L. (L.) mexicana species. Phylogenetic analysis revealed that these two species share groups of amastin surface proteins unique to the genus that could be related to specific features of disease outcomes and host cell interactions. Additionally, we describe a hypothetical hybrid interactome of potentially secreted L. (L.) amazonensis proteins and host proteins under the assumption that parasite factors mimic their mammalian counterparts. the model predicts an interaction between an L. (L.) amazonensis heat-shock protein and mammalian Toll-like receptor 9, which is implicated in important immune responses such as cytokine and nitric oxide production. the analysis presented here represents valuable information for future studies of leishmaniasis pathogenicity and treatment.Universidade Federal de São Paulo, EPM UNIFESP, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, BR-04023062 São Paulo, BrazilLNBio CNPEM, Lab Nacl Biociencias, Campinas, SP, BrazilLGE UNICAMP, Lab Genom & Expressao, Campinas, SP, BrazilInst Agron Campinas, Ctr Pesquisa & Desenvolvimento Recursos Geneti Ve, Campinas, SP, BrazilUniv Calif San Diego, Sch Med, Dept Pediat, San Diego, CA 92103 USAUniversidade Federal de São Paulo, UNIFESP, Dept Ciencia & Tecnol, Sao Jose Dos Campos, BrazilUniv N Carolina, Sch Med, Dept Genet, Chapel Hill, NC USAUniv Fed Minas Gerais, ICB UFMG, Inst Ciencias Biol, Dept Biol Geral, Belo Horizonte, MG, BrazilUniversidade Federal de São Paulo, EPM UNIFESP, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, BR-04023062 São Paulo, BrazilUniversidade Federal de São Paulo, UNIFESP, Dept Ciencia & Tecnol, Sao Jose Dos Campos, BrazilWeb of ScienceFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)FAPESP: 07/50551-2FAPESP: 10/19335-4Oxford Univ PressUniversidade Federal de São Paulo (UNIFESP)LNBio CNPEMUniversidade Estadual de Campinas (UNICAMP)Inst Agron CampinasUniv Calif San DiegoUniv N CarolinaUniversidade Federal de Minas Gerais (UFMG)Real, Fernando [UNIFESP]Vidal, Ramon OliveiraCarazzolle, Marcelo FalsarellaMondego, Jorge Mauricio CostaCosta, Gustavo Gilson LacerdaHerai, Roberto HirochiWurtele, Martin [UNIFESP]Carvalho, Lucas Miguel deFerreira, Renata Carmona [UNIFESP]Mortara, Renato Arruda [UNIFESP]Barbieri, Clara Lucia [UNIFESP]Mieczkowski, PiotrSilveira, Jose Franco da [UNIFESP]Briones, Marcelo Ribeiro da Silva [UNIFESP]Pereira, Goncalo Amarante GuimaraesBahia, Diana [UNIFESP]2016-01-24T14:34:45Z2016-01-24T14:34:45Z2013-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion567-581application/pdfhttp://dx.doi.org/10.1093/dnares/dst031Dna Research. Oxford: Oxford Univ Press, v. 20, n. 6, p. 567-581, 2013.10.1093/dnares/dst031WOS000330168100004.pdf1340-2838http://repositorio.unifesp.br/handle/11600/37004WOS:000330168100004engDna Researchinfo:eu-repo/semantics/openAccesshttp://www.oxfordjournals.org/access_purchase/self-archiving_policyb.htmlreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-07-31T16:24:03Zoai:repositorio.unifesp.br/:11600/37004Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-07-31T16:24:03Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
The Genome Sequence of Leishmania (Leishmania) amazonensis: Functional Annotation and Extended Analysis of Gene Models |
title |
The Genome Sequence of Leishmania (Leishmania) amazonensis: Functional Annotation and Extended Analysis of Gene Models |
spellingShingle |
The Genome Sequence of Leishmania (Leishmania) amazonensis: Functional Annotation and Extended Analysis of Gene Models Real, Fernando [UNIFESP] genome Leishmania amazonensis interactome amastin heat-shock protein |
title_short |
The Genome Sequence of Leishmania (Leishmania) amazonensis: Functional Annotation and Extended Analysis of Gene Models |
title_full |
The Genome Sequence of Leishmania (Leishmania) amazonensis: Functional Annotation and Extended Analysis of Gene Models |
title_fullStr |
The Genome Sequence of Leishmania (Leishmania) amazonensis: Functional Annotation and Extended Analysis of Gene Models |
title_full_unstemmed |
The Genome Sequence of Leishmania (Leishmania) amazonensis: Functional Annotation and Extended Analysis of Gene Models |
title_sort |
The Genome Sequence of Leishmania (Leishmania) amazonensis: Functional Annotation and Extended Analysis of Gene Models |
author |
Real, Fernando [UNIFESP] |
author_facet |
Real, Fernando [UNIFESP] Vidal, Ramon Oliveira Carazzolle, Marcelo Falsarella Mondego, Jorge Mauricio Costa Costa, Gustavo Gilson Lacerda Herai, Roberto Hirochi Wurtele, Martin [UNIFESP] Carvalho, Lucas Miguel de Ferreira, Renata Carmona [UNIFESP] Mortara, Renato Arruda [UNIFESP] Barbieri, Clara Lucia [UNIFESP] Mieczkowski, Piotr Silveira, Jose Franco da [UNIFESP] Briones, Marcelo Ribeiro da Silva [UNIFESP] Pereira, Goncalo Amarante Guimaraes Bahia, Diana [UNIFESP] |
author_role |
author |
author2 |
Vidal, Ramon Oliveira Carazzolle, Marcelo Falsarella Mondego, Jorge Mauricio Costa Costa, Gustavo Gilson Lacerda Herai, Roberto Hirochi Wurtele, Martin [UNIFESP] Carvalho, Lucas Miguel de Ferreira, Renata Carmona [UNIFESP] Mortara, Renato Arruda [UNIFESP] Barbieri, Clara Lucia [UNIFESP] Mieczkowski, Piotr Silveira, Jose Franco da [UNIFESP] Briones, Marcelo Ribeiro da Silva [UNIFESP] Pereira, Goncalo Amarante Guimaraes Bahia, Diana [UNIFESP] |
author2_role |
author author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) LNBio CNPEM Universidade Estadual de Campinas (UNICAMP) Inst Agron Campinas Univ Calif San Diego Univ N Carolina Universidade Federal de Minas Gerais (UFMG) |
dc.contributor.author.fl_str_mv |
Real, Fernando [UNIFESP] Vidal, Ramon Oliveira Carazzolle, Marcelo Falsarella Mondego, Jorge Mauricio Costa Costa, Gustavo Gilson Lacerda Herai, Roberto Hirochi Wurtele, Martin [UNIFESP] Carvalho, Lucas Miguel de Ferreira, Renata Carmona [UNIFESP] Mortara, Renato Arruda [UNIFESP] Barbieri, Clara Lucia [UNIFESP] Mieczkowski, Piotr Silveira, Jose Franco da [UNIFESP] Briones, Marcelo Ribeiro da Silva [UNIFESP] Pereira, Goncalo Amarante Guimaraes Bahia, Diana [UNIFESP] |
dc.subject.por.fl_str_mv |
genome Leishmania amazonensis interactome amastin heat-shock protein |
topic |
genome Leishmania amazonensis interactome amastin heat-shock protein |
description |
We present the sequencing and annotation of the Leishmania (Leishmania) amazonensis genome, an etiological agent of human cutaneous leishmaniasis in the Amazon region of Brazil. L. (L.) amazonensis shares features with Leishmania (L.) mexicana but also exhibits unique characteristics regarding geographical distribution and clinical manifestations of cutaneous lesions (e.g. borderline disseminated cutaneous leishmaniasis). Predicted genes were scored for orthologous gene families and conserved domains in comparison with other human pathogenic Leishmania spp. Carboxypeptidase, aminotransferase, and 3'-nucleotidase genes and ATPase, thioredoxin, and chaperone-related domains were represented more abundantly in L. (L.) amazonensis and L. (L.) mexicana species. Phylogenetic analysis revealed that these two species share groups of amastin surface proteins unique to the genus that could be related to specific features of disease outcomes and host cell interactions. Additionally, we describe a hypothetical hybrid interactome of potentially secreted L. (L.) amazonensis proteins and host proteins under the assumption that parasite factors mimic their mammalian counterparts. the model predicts an interaction between an L. (L.) amazonensis heat-shock protein and mammalian Toll-like receptor 9, which is implicated in important immune responses such as cytokine and nitric oxide production. the analysis presented here represents valuable information for future studies of leishmaniasis pathogenicity and treatment. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-12-01 2016-01-24T14:34:45Z 2016-01-24T14:34:45Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1093/dnares/dst031 Dna Research. Oxford: Oxford Univ Press, v. 20, n. 6, p. 567-581, 2013. 10.1093/dnares/dst031 WOS000330168100004.pdf 1340-2838 http://repositorio.unifesp.br/handle/11600/37004 WOS:000330168100004 |
url |
http://dx.doi.org/10.1093/dnares/dst031 http://repositorio.unifesp.br/handle/11600/37004 |
identifier_str_mv |
Dna Research. Oxford: Oxford Univ Press, v. 20, n. 6, p. 567-581, 2013. 10.1093/dnares/dst031 WOS000330168100004.pdf 1340-2838 WOS:000330168100004 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Dna Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess http://www.oxfordjournals.org/access_purchase/self-archiving_policyb.html |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://www.oxfordjournals.org/access_purchase/self-archiving_policyb.html |
dc.format.none.fl_str_mv |
567-581 application/pdf |
dc.publisher.none.fl_str_mv |
Oxford Univ Press |
publisher.none.fl_str_mv |
Oxford Univ Press |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
_version_ |
1814268383464521728 |