The Genome Sequence of Leishmania (Leishmania) amazonensis: Functional Annotation and Extended Analysis of Gene Models

Detalhes bibliográficos
Autor(a) principal: Real, Fernando [UNIFESP]
Data de Publicação: 2013
Outros Autores: Vidal, Ramon Oliveira, Carazzolle, Marcelo Falsarella, Mondego, Jorge Mauricio Costa, Costa, Gustavo Gilson Lacerda, Herai, Roberto Hirochi, Wurtele, Martin [UNIFESP], Carvalho, Lucas Miguel de, Ferreira, Renata Carmona [UNIFESP], Mortara, Renato Arruda [UNIFESP], Barbieri, Clara Lucia [UNIFESP], Mieczkowski, Piotr, Silveira, Jose Franco da [UNIFESP], Briones, Marcelo Ribeiro da Silva [UNIFESP], Pereira, Goncalo Amarante Guimaraes, Bahia, Diana [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1093/dnares/dst031
http://repositorio.unifesp.br/handle/11600/37004
Resumo: We present the sequencing and annotation of the Leishmania (Leishmania) amazonensis genome, an etiological agent of human cutaneous leishmaniasis in the Amazon region of Brazil. L. (L.) amazonensis shares features with Leishmania (L.) mexicana but also exhibits unique characteristics regarding geographical distribution and clinical manifestations of cutaneous lesions (e.g. borderline disseminated cutaneous leishmaniasis). Predicted genes were scored for orthologous gene families and conserved domains in comparison with other human pathogenic Leishmania spp. Carboxypeptidase, aminotransferase, and 3'-nucleotidase genes and ATPase, thioredoxin, and chaperone-related domains were represented more abundantly in L. (L.) amazonensis and L. (L.) mexicana species. Phylogenetic analysis revealed that these two species share groups of amastin surface proteins unique to the genus that could be related to specific features of disease outcomes and host cell interactions. Additionally, we describe a hypothetical hybrid interactome of potentially secreted L. (L.) amazonensis proteins and host proteins under the assumption that parasite factors mimic their mammalian counterparts. the model predicts an interaction between an L. (L.) amazonensis heat-shock protein and mammalian Toll-like receptor 9, which is implicated in important immune responses such as cytokine and nitric oxide production. the analysis presented here represents valuable information for future studies of leishmaniasis pathogenicity and treatment.
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spelling The Genome Sequence of Leishmania (Leishmania) amazonensis: Functional Annotation and Extended Analysis of Gene ModelsgenomeLeishmania amazonensisinteractomeamastinheat-shock proteinWe present the sequencing and annotation of the Leishmania (Leishmania) amazonensis genome, an etiological agent of human cutaneous leishmaniasis in the Amazon region of Brazil. L. (L.) amazonensis shares features with Leishmania (L.) mexicana but also exhibits unique characteristics regarding geographical distribution and clinical manifestations of cutaneous lesions (e.g. borderline disseminated cutaneous leishmaniasis). Predicted genes were scored for orthologous gene families and conserved domains in comparison with other human pathogenic Leishmania spp. Carboxypeptidase, aminotransferase, and 3'-nucleotidase genes and ATPase, thioredoxin, and chaperone-related domains were represented more abundantly in L. (L.) amazonensis and L. (L.) mexicana species. Phylogenetic analysis revealed that these two species share groups of amastin surface proteins unique to the genus that could be related to specific features of disease outcomes and host cell interactions. Additionally, we describe a hypothetical hybrid interactome of potentially secreted L. (L.) amazonensis proteins and host proteins under the assumption that parasite factors mimic their mammalian counterparts. the model predicts an interaction between an L. (L.) amazonensis heat-shock protein and mammalian Toll-like receptor 9, which is implicated in important immune responses such as cytokine and nitric oxide production. the analysis presented here represents valuable information for future studies of leishmaniasis pathogenicity and treatment.Universidade Federal de São Paulo, EPM UNIFESP, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, BR-04023062 São Paulo, BrazilLNBio CNPEM, Lab Nacl Biociencias, Campinas, SP, BrazilLGE UNICAMP, Lab Genom & Expressao, Campinas, SP, BrazilInst Agron Campinas, Ctr Pesquisa & Desenvolvimento Recursos Geneti Ve, Campinas, SP, BrazilUniv Calif San Diego, Sch Med, Dept Pediat, San Diego, CA 92103 USAUniversidade Federal de São Paulo, UNIFESP, Dept Ciencia & Tecnol, Sao Jose Dos Campos, BrazilUniv N Carolina, Sch Med, Dept Genet, Chapel Hill, NC USAUniv Fed Minas Gerais, ICB UFMG, Inst Ciencias Biol, Dept Biol Geral, Belo Horizonte, MG, BrazilUniversidade Federal de São Paulo, EPM UNIFESP, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, BR-04023062 São Paulo, BrazilUniversidade Federal de São Paulo, UNIFESP, Dept Ciencia & Tecnol, Sao Jose Dos Campos, BrazilWeb of ScienceFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)FAPESP: 07/50551-2FAPESP: 10/19335-4Oxford Univ PressUniversidade Federal de São Paulo (UNIFESP)LNBio CNPEMUniversidade Estadual de Campinas (UNICAMP)Inst Agron CampinasUniv Calif San DiegoUniv N CarolinaUniversidade Federal de Minas Gerais (UFMG)Real, Fernando [UNIFESP]Vidal, Ramon OliveiraCarazzolle, Marcelo FalsarellaMondego, Jorge Mauricio CostaCosta, Gustavo Gilson LacerdaHerai, Roberto HirochiWurtele, Martin [UNIFESP]Carvalho, Lucas Miguel deFerreira, Renata Carmona [UNIFESP]Mortara, Renato Arruda [UNIFESP]Barbieri, Clara Lucia [UNIFESP]Mieczkowski, PiotrSilveira, Jose Franco da [UNIFESP]Briones, Marcelo Ribeiro da Silva [UNIFESP]Pereira, Goncalo Amarante GuimaraesBahia, Diana [UNIFESP]2016-01-24T14:34:45Z2016-01-24T14:34:45Z2013-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion567-581application/pdfhttp://dx.doi.org/10.1093/dnares/dst031Dna Research. Oxford: Oxford Univ Press, v. 20, n. 6, p. 567-581, 2013.10.1093/dnares/dst031WOS000330168100004.pdf1340-2838http://repositorio.unifesp.br/handle/11600/37004WOS:000330168100004engDna Researchinfo:eu-repo/semantics/openAccesshttp://www.oxfordjournals.org/access_purchase/self-archiving_policyb.htmlreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-07-31T16:24:03Zoai:repositorio.unifesp.br/:11600/37004Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-07-31T16:24:03Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv The Genome Sequence of Leishmania (Leishmania) amazonensis: Functional Annotation and Extended Analysis of Gene Models
title The Genome Sequence of Leishmania (Leishmania) amazonensis: Functional Annotation and Extended Analysis of Gene Models
spellingShingle The Genome Sequence of Leishmania (Leishmania) amazonensis: Functional Annotation and Extended Analysis of Gene Models
Real, Fernando [UNIFESP]
genome
Leishmania amazonensis
interactome
amastin
heat-shock protein
title_short The Genome Sequence of Leishmania (Leishmania) amazonensis: Functional Annotation and Extended Analysis of Gene Models
title_full The Genome Sequence of Leishmania (Leishmania) amazonensis: Functional Annotation and Extended Analysis of Gene Models
title_fullStr The Genome Sequence of Leishmania (Leishmania) amazonensis: Functional Annotation and Extended Analysis of Gene Models
title_full_unstemmed The Genome Sequence of Leishmania (Leishmania) amazonensis: Functional Annotation and Extended Analysis of Gene Models
title_sort The Genome Sequence of Leishmania (Leishmania) amazonensis: Functional Annotation and Extended Analysis of Gene Models
author Real, Fernando [UNIFESP]
author_facet Real, Fernando [UNIFESP]
Vidal, Ramon Oliveira
Carazzolle, Marcelo Falsarella
Mondego, Jorge Mauricio Costa
Costa, Gustavo Gilson Lacerda
Herai, Roberto Hirochi
Wurtele, Martin [UNIFESP]
Carvalho, Lucas Miguel de
Ferreira, Renata Carmona [UNIFESP]
Mortara, Renato Arruda [UNIFESP]
Barbieri, Clara Lucia [UNIFESP]
Mieczkowski, Piotr
Silveira, Jose Franco da [UNIFESP]
Briones, Marcelo Ribeiro da Silva [UNIFESP]
Pereira, Goncalo Amarante Guimaraes
Bahia, Diana [UNIFESP]
author_role author
author2 Vidal, Ramon Oliveira
Carazzolle, Marcelo Falsarella
Mondego, Jorge Mauricio Costa
Costa, Gustavo Gilson Lacerda
Herai, Roberto Hirochi
Wurtele, Martin [UNIFESP]
Carvalho, Lucas Miguel de
Ferreira, Renata Carmona [UNIFESP]
Mortara, Renato Arruda [UNIFESP]
Barbieri, Clara Lucia [UNIFESP]
Mieczkowski, Piotr
Silveira, Jose Franco da [UNIFESP]
Briones, Marcelo Ribeiro da Silva [UNIFESP]
Pereira, Goncalo Amarante Guimaraes
Bahia, Diana [UNIFESP]
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
LNBio CNPEM
Universidade Estadual de Campinas (UNICAMP)
Inst Agron Campinas
Univ Calif San Diego
Univ N Carolina
Universidade Federal de Minas Gerais (UFMG)
dc.contributor.author.fl_str_mv Real, Fernando [UNIFESP]
Vidal, Ramon Oliveira
Carazzolle, Marcelo Falsarella
Mondego, Jorge Mauricio Costa
Costa, Gustavo Gilson Lacerda
Herai, Roberto Hirochi
Wurtele, Martin [UNIFESP]
Carvalho, Lucas Miguel de
Ferreira, Renata Carmona [UNIFESP]
Mortara, Renato Arruda [UNIFESP]
Barbieri, Clara Lucia [UNIFESP]
Mieczkowski, Piotr
Silveira, Jose Franco da [UNIFESP]
Briones, Marcelo Ribeiro da Silva [UNIFESP]
Pereira, Goncalo Amarante Guimaraes
Bahia, Diana [UNIFESP]
dc.subject.por.fl_str_mv genome
Leishmania amazonensis
interactome
amastin
heat-shock protein
topic genome
Leishmania amazonensis
interactome
amastin
heat-shock protein
description We present the sequencing and annotation of the Leishmania (Leishmania) amazonensis genome, an etiological agent of human cutaneous leishmaniasis in the Amazon region of Brazil. L. (L.) amazonensis shares features with Leishmania (L.) mexicana but also exhibits unique characteristics regarding geographical distribution and clinical manifestations of cutaneous lesions (e.g. borderline disseminated cutaneous leishmaniasis). Predicted genes were scored for orthologous gene families and conserved domains in comparison with other human pathogenic Leishmania spp. Carboxypeptidase, aminotransferase, and 3'-nucleotidase genes and ATPase, thioredoxin, and chaperone-related domains were represented more abundantly in L. (L.) amazonensis and L. (L.) mexicana species. Phylogenetic analysis revealed that these two species share groups of amastin surface proteins unique to the genus that could be related to specific features of disease outcomes and host cell interactions. Additionally, we describe a hypothetical hybrid interactome of potentially secreted L. (L.) amazonensis proteins and host proteins under the assumption that parasite factors mimic their mammalian counterparts. the model predicts an interaction between an L. (L.) amazonensis heat-shock protein and mammalian Toll-like receptor 9, which is implicated in important immune responses such as cytokine and nitric oxide production. the analysis presented here represents valuable information for future studies of leishmaniasis pathogenicity and treatment.
publishDate 2013
dc.date.none.fl_str_mv 2013-12-01
2016-01-24T14:34:45Z
2016-01-24T14:34:45Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1093/dnares/dst031
Dna Research. Oxford: Oxford Univ Press, v. 20, n. 6, p. 567-581, 2013.
10.1093/dnares/dst031
WOS000330168100004.pdf
1340-2838
http://repositorio.unifesp.br/handle/11600/37004
WOS:000330168100004
url http://dx.doi.org/10.1093/dnares/dst031
http://repositorio.unifesp.br/handle/11600/37004
identifier_str_mv Dna Research. Oxford: Oxford Univ Press, v. 20, n. 6, p. 567-581, 2013.
10.1093/dnares/dst031
WOS000330168100004.pdf
1340-2838
WOS:000330168100004
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Dna Research
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
http://www.oxfordjournals.org/access_purchase/self-archiving_policyb.html
eu_rights_str_mv openAccess
rights_invalid_str_mv http://www.oxfordjournals.org/access_purchase/self-archiving_policyb.html
dc.format.none.fl_str_mv 567-581
application/pdf
dc.publisher.none.fl_str_mv Oxford Univ Press
publisher.none.fl_str_mv Oxford Univ Press
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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