MYCN gene amplification - Identification of cell populations containing double minutes and homogeneously staining regions in neuroblastoma tumors
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 1999 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UNIFESP |
| Texto Completo: | http://dx.doi.org/10.1016/S0002-9440(10)65457-0 http://repositorio.unifesp.br/handle/11600/26163 |
Resumo: | Neuroblastoma is the second most common solid tumor occurring in children. Amplification of the MYCN oncogene is associated with poor prognosis. To identify neuroblastoma tumors with MYCN amplification, we studied the number of copies of MYCN in interphase cells by fluorescence in situ hybridization in 20 neuroblastoma patients. MYCN amplification appeared in 7 tumor specimens. Interphase and metaphase studies showed a tumor cell population with both forms of amplification, double minutes and homogeneously staining regions, in two patients. These patients showed a smaller tumor cell subpopulation with the presence of more than one homogeneously staining region, suggesting that gene amplification was undergoing karyotype evolution. |
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MYCN gene amplification - Identification of cell populations containing double minutes and homogeneously staining regions in neuroblastoma tumorsNeuroblastoma is the second most common solid tumor occurring in children. Amplification of the MYCN oncogene is associated with poor prognosis. To identify neuroblastoma tumors with MYCN amplification, we studied the number of copies of MYCN in interphase cells by fluorescence in situ hybridization in 20 neuroblastoma patients. MYCN amplification appeared in 7 tumor specimens. Interphase and metaphase studies showed a tumor cell population with both forms of amplification, double minutes and homogeneously staining regions, in two patients. These patients showed a smaller tumor cell subpopulation with the presence of more than one homogeneously staining region, suggesting that gene amplification was undergoing karyotype evolution.Universidade Federal de São Paulo, Dept Morphol, Div Genet, Escola Paulista Med, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Pathol, Div Med Pathol, Escola Paulista Med, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Surg, Div Pediat Surg, Escola Paulista Med, São Paulo, BrazilUniversidade Federal de São Paulo, Inst Oncol Pediat, Escola Paulista Med, São Paulo, BrazilHosp Servidor Publ Estadual, Div Pediat Oncol, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Morphol, Div Genet, Escola Paulista Med, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Pathol, Div Med Pathol, Escola Paulista Med, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Surg, Div Pediat Surg, Escola Paulista Med, São Paulo, BrazilUniversidade Federal de São Paulo, Inst Oncol Pediat, Escola Paulista Med, São Paulo, BrazilWeb of ScienceAmer Soc Investigative Pathology, IncUniversidade Federal de São Paulo (UNIFESP)Hosp Servidor Publ EstadualYoshimoto, Maisa [UNIFESP]Toledo, Silvia Regina Caminada de [UNIFESP]Caran, Eliana Maria Monteiro [UNIFESP]Seixas, Maria Teresa de [UNIFESP]Lee, Maria Lucia de Martino [UNIFESP]Abib, Simone de Campos Vieira [UNIFESP]Vianna, Sonia Maria RossiSchettini, Sergio Tomaz [UNIFESP]Andrade, Joyce Anderson Duffles [UNIFESP]2016-01-24T12:30:54Z2016-01-24T12:30:54Z1999-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion1439-1443http://dx.doi.org/10.1016/S0002-9440(10)65457-0American Journal of Pathology. Baltimore: Amer Soc Investigative Pathology, Inc, v. 155, n. 5, p. 1439-1443, 1999.10.1016/S0002-9440(10)65457-00002-9440http://repositorio.unifesp.br/handle/11600/26163WOS:000083587600007engAmerican Journal of Pathologyinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2016-03-16T15:49:48Zoai:repositorio.unifesp.br/:11600/26163Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652016-03-16T15:49:48Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
| dc.title.none.fl_str_mv |
MYCN gene amplification - Identification of cell populations containing double minutes and homogeneously staining regions in neuroblastoma tumors |
| title |
MYCN gene amplification - Identification of cell populations containing double minutes and homogeneously staining regions in neuroblastoma tumors |
| spellingShingle |
MYCN gene amplification - Identification of cell populations containing double minutes and homogeneously staining regions in neuroblastoma tumors Yoshimoto, Maisa [UNIFESP] |
| title_short |
MYCN gene amplification - Identification of cell populations containing double minutes and homogeneously staining regions in neuroblastoma tumors |
| title_full |
MYCN gene amplification - Identification of cell populations containing double minutes and homogeneously staining regions in neuroblastoma tumors |
| title_fullStr |
MYCN gene amplification - Identification of cell populations containing double minutes and homogeneously staining regions in neuroblastoma tumors |
| title_full_unstemmed |
MYCN gene amplification - Identification of cell populations containing double minutes and homogeneously staining regions in neuroblastoma tumors |
| title_sort |
MYCN gene amplification - Identification of cell populations containing double minutes and homogeneously staining regions in neuroblastoma tumors |
| author |
Yoshimoto, Maisa [UNIFESP] |
| author_facet |
Yoshimoto, Maisa [UNIFESP] Toledo, Silvia Regina Caminada de [UNIFESP] Caran, Eliana Maria Monteiro [UNIFESP] Seixas, Maria Teresa de [UNIFESP] Lee, Maria Lucia de Martino [UNIFESP] Abib, Simone de Campos Vieira [UNIFESP] Vianna, Sonia Maria Rossi Schettini, Sergio Tomaz [UNIFESP] Andrade, Joyce Anderson Duffles [UNIFESP] |
| author_role |
author |
| author2 |
Toledo, Silvia Regina Caminada de [UNIFESP] Caran, Eliana Maria Monteiro [UNIFESP] Seixas, Maria Teresa de [UNIFESP] Lee, Maria Lucia de Martino [UNIFESP] Abib, Simone de Campos Vieira [UNIFESP] Vianna, Sonia Maria Rossi Schettini, Sergio Tomaz [UNIFESP] Andrade, Joyce Anderson Duffles [UNIFESP] |
| author2_role |
author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Hosp Servidor Publ Estadual |
| dc.contributor.author.fl_str_mv |
Yoshimoto, Maisa [UNIFESP] Toledo, Silvia Regina Caminada de [UNIFESP] Caran, Eliana Maria Monteiro [UNIFESP] Seixas, Maria Teresa de [UNIFESP] Lee, Maria Lucia de Martino [UNIFESP] Abib, Simone de Campos Vieira [UNIFESP] Vianna, Sonia Maria Rossi Schettini, Sergio Tomaz [UNIFESP] Andrade, Joyce Anderson Duffles [UNIFESP] |
| description |
Neuroblastoma is the second most common solid tumor occurring in children. Amplification of the MYCN oncogene is associated with poor prognosis. To identify neuroblastoma tumors with MYCN amplification, we studied the number of copies of MYCN in interphase cells by fluorescence in situ hybridization in 20 neuroblastoma patients. MYCN amplification appeared in 7 tumor specimens. Interphase and metaphase studies showed a tumor cell population with both forms of amplification, double minutes and homogeneously staining regions, in two patients. These patients showed a smaller tumor cell subpopulation with the presence of more than one homogeneously staining region, suggesting that gene amplification was undergoing karyotype evolution. |
| publishDate |
1999 |
| dc.date.none.fl_str_mv |
1999-11-01 2016-01-24T12:30:54Z 2016-01-24T12:30:54Z |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/S0002-9440(10)65457-0 American Journal of Pathology. Baltimore: Amer Soc Investigative Pathology, Inc, v. 155, n. 5, p. 1439-1443, 1999. 10.1016/S0002-9440(10)65457-0 0002-9440 http://repositorio.unifesp.br/handle/11600/26163 WOS:000083587600007 |
| url |
http://dx.doi.org/10.1016/S0002-9440(10)65457-0 http://repositorio.unifesp.br/handle/11600/26163 |
| identifier_str_mv |
American Journal of Pathology. Baltimore: Amer Soc Investigative Pathology, Inc, v. 155, n. 5, p. 1439-1443, 1999. 10.1016/S0002-9440(10)65457-0 0002-9440 WOS:000083587600007 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
American Journal of Pathology |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
1439-1443 |
| dc.publisher.none.fl_str_mv |
Amer Soc Investigative Pathology, Inc |
| publisher.none.fl_str_mv |
Amer Soc Investigative Pathology, Inc |
| dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
| instname_str |
Universidade Federal de São Paulo (UNIFESP) |
| instacron_str |
UNIFESP |
| institution |
UNIFESP |
| reponame_str |
Repositório Institucional da UNIFESP |
| collection |
Repositório Institucional da UNIFESP |
| repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
| repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
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1814268383307235328 |