Cell proliferation and apoptosis in gastric cancer and intestinal metaplasia

Detalhes bibliográficos
Autor(a) principal: Forones, Nora Manoukian [UNIFESP]
Data de Publicação: 2005
Outros Autores: Carvalho, Ana Paula Souza, Giannotti-Filho, Oswaldo, Lourenço, Laércio Gomes [UNIFESP], Oshima, Celina Tizuko Fujiyama [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
dARK ID: ark:/48912/0013000012kzd
Texto Completo: http://dx.doi.org/10.1590/S0004-28032005000100008
http://repositorio.unifesp.br/handle/11600/2442
Resumo: BACKGROUND: Higher proliferation is commonly observed in cancer cells. Apoptosis can be a useful measure of a tumor cell kinetic. Alteration of the balance between proliferation and apoptosis is associated with cancer. AIM: To study proliferation and apoptosis on gastric cancer and in intestinal metaplasia. METHODOLOGY: Twenty-two samples from gastric adenocarcinomas and 22 biopsies from intestinal metaplasia were studied. The apoptotic bodies in hematoxylin-eosin slides and the expression of p53, bcl-2 and Ki67 were determined by immunohistochemistry. RESULTS: The number of the apoptotic cells was higher in cancer. Ki 67LI increased from intestinal metaplasia to gastric cancer. p53 was positive in 68% of the patients with cancer, more frequently in advanced stage and negative in samples of intestinal metaplasia. Although there was no significant difference between the groups, bcl-2 was positive in 45% of gastric cancer tissue and in 68% of metaplasia. In gastric cancer patients bcl-2 was expressed in early gastric cancer more frequently than in advanced stage. CONCLUSION: The positivity of bcl-2 was higher in metaplasia and probably is involved in the progression of carcinogenesis. p53 was negative in metaplasia and positive in more than half of the gastric cancer, mostly in stage IV, suggesting a late event in gastric cancer.
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spelling Cell proliferation and apoptosis in gastric cancer and intestinal metaplasiaProliferação celular e apoptose em câncer gástrico e metaplasia intestinalApoptosisProtein p53Proto-oncogene proteinsProto-oncogene proteins c-Bcl-2Stomach neoplasmsMetaplasiaApoptoseProteína p53Proteínas proto-oncogênicasProteínas proto-oncogênicas c-Bcl-2Neoplasias gástricasMetaplasiaBACKGROUND: Higher proliferation is commonly observed in cancer cells. Apoptosis can be a useful measure of a tumor cell kinetic. Alteration of the balance between proliferation and apoptosis is associated with cancer. AIM: To study proliferation and apoptosis on gastric cancer and in intestinal metaplasia. METHODOLOGY: Twenty-two samples from gastric adenocarcinomas and 22 biopsies from intestinal metaplasia were studied. The apoptotic bodies in hematoxylin-eosin slides and the expression of p53, bcl-2 and Ki67 were determined by immunohistochemistry. RESULTS: The number of the apoptotic cells was higher in cancer. Ki 67LI increased from intestinal metaplasia to gastric cancer. p53 was positive in 68% of the patients with cancer, more frequently in advanced stage and negative in samples of intestinal metaplasia. Although there was no significant difference between the groups, bcl-2 was positive in 45% of gastric cancer tissue and in 68% of metaplasia. In gastric cancer patients bcl-2 was expressed in early gastric cancer more frequently than in advanced stage. CONCLUSION: The positivity of bcl-2 was higher in metaplasia and probably is involved in the progression of carcinogenesis. p53 was negative in metaplasia and positive in more than half of the gastric cancer, mostly in stage IV, suggesting a late event in gastric cancer.RACIONAL: O aumento da proliferação celular é comumente observado no câncer e a apoptose pode ser medida útil na avaliação da cinética celular do tumor. Alterações do equilíbrio entre proliferação celular e apoptose estão associadas ao câncer. OBJETIVO: Avaliar a proliferação celular e a apoptose no câncer gástrico e na metaplasia intestinal. CASUÍSTICA E MÉTODOS: Vinte e dois tecidos de adenocarcinoma gástrico e 22 biopsias de metaplasia intestinal foram estudados. Os corpos apoptóticos foram estudados em lâminas coradas pela hematoxilina-eosina e a expressão de p53, bcl-2 e Ki67 por imunoistoquímica. RESULTADOS: O número de corpos apoptóticos foi maior no câncer. O Ki67LI aumentou no câncer gástrico em relação ao tecido com metaplasia intestinal. O p53 foi positivo em 68% dos pacientes com câncer, principalmente nos tumores avançados e negativo nos indivíduos com metaplasia intestinal. O bcl-2 foi expresso em 45% dos tecidos com câncer gástrico e em 68% dos tecidos com metaplasia, no entanto, esta diferença não foi significante. Nos doentes com câncer gástrico precoce, o bcl-2 foi expresso com maior freqüência quando comparados aos com câncer em estádio avançado. CONCLUSÃO: A expressão do bcl-2 foi maior no tecido com metaplasia, sugerindo envolvimento desta proteína na evolução da carcinogênese. O p53 foi negativo nos tecidos com metaplasia intestinal e positivo em 50% dos tecidos tumorais, principalmente nos doentes com câncer estádio IV, sugerindo ser um evento tardio no câncer gástrico.IOncology Group Gastroenterology DivisionPathology DepartmentFederal University of São Paulo Surgical Gastroenterology DivisionUNIFESP, Surgical Gastroenterology DivisionSciELOInstituto Brasileiro de Estudos e Pesquisas de Gastroenterologia - IBEPEGE Colégio Brasileiro de Cirurgia Digestiva - CBCD Sociedade Brasileira de Motilidade Digestiva - SBMD Federação Brasileira de Gastroenterologia - FBGSociedade Brasileira de Hepatologia - SBHSociedade Brasileira de Endoscopia Digestiva - SOBEDIOncology Group Gastroenterology DivisionPathology DepartmentUniversidade Federal de São Paulo (UNIFESP)Forones, Nora Manoukian [UNIFESP]Carvalho, Ana Paula SouzaGiannotti-Filho, OswaldoLourenço, Laércio Gomes [UNIFESP]Oshima, Celina Tizuko Fujiyama [UNIFESP]2015-06-14T13:31:29Z2015-06-14T13:31:29Z2005-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion30-34application/pdfhttp://dx.doi.org/10.1590/S0004-28032005000100008Arquivos de Gastroenterologia. Instituto Brasileiro de Estudos e Pesquisas de Gastroenterologia - IBEPEGE Colégio Brasileiro de Cirurgia Digestiva - CBCD Sociedade Brasileira de Motilidade Digestiva - SBMD Federação Brasileira de Gastroenterologia - FBGSociedade Brasileira de Hepatologia - SBHSociedade Brasileira de Endoscopia Digestiva - SOBED, v. 42, n. 1, p. 30-34, 2005.10.1590/S0004-28032005000100008S0004-28032005000100008.pdf0004-2803S0004-28032005000100008http://repositorio.unifesp.br/handle/11600/2442ark:/48912/0013000012kzdengArquivos de Gastroenterologiainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-05T17:16:00Zoai:repositorio.unifesp.br/:11600/2442Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-12-11T20:51:41.576853Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Cell proliferation and apoptosis in gastric cancer and intestinal metaplasia
Proliferação celular e apoptose em câncer gástrico e metaplasia intestinal
title Cell proliferation and apoptosis in gastric cancer and intestinal metaplasia
spellingShingle Cell proliferation and apoptosis in gastric cancer and intestinal metaplasia
Forones, Nora Manoukian [UNIFESP]
Apoptosis
Protein p53
Proto-oncogene proteins
Proto-oncogene proteins c-Bcl-2
Stomach neoplasms
Metaplasia
Apoptose
Proteína p53
Proteínas proto-oncogênicas
Proteínas proto-oncogênicas c-Bcl-2
Neoplasias gástricas
Metaplasia
title_short Cell proliferation and apoptosis in gastric cancer and intestinal metaplasia
title_full Cell proliferation and apoptosis in gastric cancer and intestinal metaplasia
title_fullStr Cell proliferation and apoptosis in gastric cancer and intestinal metaplasia
title_full_unstemmed Cell proliferation and apoptosis in gastric cancer and intestinal metaplasia
title_sort Cell proliferation and apoptosis in gastric cancer and intestinal metaplasia
author Forones, Nora Manoukian [UNIFESP]
author_facet Forones, Nora Manoukian [UNIFESP]
Carvalho, Ana Paula Souza
Giannotti-Filho, Oswaldo
Lourenço, Laércio Gomes [UNIFESP]
Oshima, Celina Tizuko Fujiyama [UNIFESP]
author_role author
author2 Carvalho, Ana Paula Souza
Giannotti-Filho, Oswaldo
Lourenço, Laércio Gomes [UNIFESP]
Oshima, Celina Tizuko Fujiyama [UNIFESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv IOncology Group Gastroenterology Division
Pathology Department
Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Forones, Nora Manoukian [UNIFESP]
Carvalho, Ana Paula Souza
Giannotti-Filho, Oswaldo
Lourenço, Laércio Gomes [UNIFESP]
Oshima, Celina Tizuko Fujiyama [UNIFESP]
dc.subject.por.fl_str_mv Apoptosis
Protein p53
Proto-oncogene proteins
Proto-oncogene proteins c-Bcl-2
Stomach neoplasms
Metaplasia
Apoptose
Proteína p53
Proteínas proto-oncogênicas
Proteínas proto-oncogênicas c-Bcl-2
Neoplasias gástricas
Metaplasia
topic Apoptosis
Protein p53
Proto-oncogene proteins
Proto-oncogene proteins c-Bcl-2
Stomach neoplasms
Metaplasia
Apoptose
Proteína p53
Proteínas proto-oncogênicas
Proteínas proto-oncogênicas c-Bcl-2
Neoplasias gástricas
Metaplasia
description BACKGROUND: Higher proliferation is commonly observed in cancer cells. Apoptosis can be a useful measure of a tumor cell kinetic. Alteration of the balance between proliferation and apoptosis is associated with cancer. AIM: To study proliferation and apoptosis on gastric cancer and in intestinal metaplasia. METHODOLOGY: Twenty-two samples from gastric adenocarcinomas and 22 biopsies from intestinal metaplasia were studied. The apoptotic bodies in hematoxylin-eosin slides and the expression of p53, bcl-2 and Ki67 were determined by immunohistochemistry. RESULTS: The number of the apoptotic cells was higher in cancer. Ki 67LI increased from intestinal metaplasia to gastric cancer. p53 was positive in 68% of the patients with cancer, more frequently in advanced stage and negative in samples of intestinal metaplasia. Although there was no significant difference between the groups, bcl-2 was positive in 45% of gastric cancer tissue and in 68% of metaplasia. In gastric cancer patients bcl-2 was expressed in early gastric cancer more frequently than in advanced stage. CONCLUSION: The positivity of bcl-2 was higher in metaplasia and probably is involved in the progression of carcinogenesis. p53 was negative in metaplasia and positive in more than half of the gastric cancer, mostly in stage IV, suggesting a late event in gastric cancer.
publishDate 2005
dc.date.none.fl_str_mv 2005-03-01
2015-06-14T13:31:29Z
2015-06-14T13:31:29Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0004-28032005000100008
Arquivos de Gastroenterologia. Instituto Brasileiro de Estudos e Pesquisas de Gastroenterologia - IBEPEGE Colégio Brasileiro de Cirurgia Digestiva - CBCD Sociedade Brasileira de Motilidade Digestiva - SBMD Federação Brasileira de Gastroenterologia - FBGSociedade Brasileira de Hepatologia - SBHSociedade Brasileira de Endoscopia Digestiva - SOBED, v. 42, n. 1, p. 30-34, 2005.
10.1590/S0004-28032005000100008
S0004-28032005000100008.pdf
0004-2803
S0004-28032005000100008
http://repositorio.unifesp.br/handle/11600/2442
dc.identifier.dark.fl_str_mv ark:/48912/0013000012kzd
url http://dx.doi.org/10.1590/S0004-28032005000100008
http://repositorio.unifesp.br/handle/11600/2442
identifier_str_mv Arquivos de Gastroenterologia. Instituto Brasileiro de Estudos e Pesquisas de Gastroenterologia - IBEPEGE Colégio Brasileiro de Cirurgia Digestiva - CBCD Sociedade Brasileira de Motilidade Digestiva - SBMD Federação Brasileira de Gastroenterologia - FBGSociedade Brasileira de Hepatologia - SBHSociedade Brasileira de Endoscopia Digestiva - SOBED, v. 42, n. 1, p. 30-34, 2005.
10.1590/S0004-28032005000100008
S0004-28032005000100008.pdf
0004-2803
S0004-28032005000100008
ark:/48912/0013000012kzd
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Arquivos de Gastroenterologia
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 30-34
application/pdf
dc.publisher.none.fl_str_mv Instituto Brasileiro de Estudos e Pesquisas de Gastroenterologia - IBEPEGE Colégio Brasileiro de Cirurgia Digestiva - CBCD Sociedade Brasileira de Motilidade Digestiva - SBMD Federação Brasileira de Gastroenterologia - FBGSociedade Brasileira de Hepatologia - SBHSociedade Brasileira de Endoscopia Digestiva - SOBED
publisher.none.fl_str_mv Instituto Brasileiro de Estudos e Pesquisas de Gastroenterologia - IBEPEGE Colégio Brasileiro de Cirurgia Digestiva - CBCD Sociedade Brasileira de Motilidade Digestiva - SBMD Federação Brasileira de Gastroenterologia - FBGSociedade Brasileira de Hepatologia - SBHSociedade Brasileira de Endoscopia Digestiva - SOBED
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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