Torque Teno Virus (TTV) is Prevalent in Brazilian Non-human Primates and Chickens (Gallus gallus domesticus)

• Autor(a) principal: Catroxo, Marcia Helena Braga [UNIFESP]
• Data de Publicação: 2008
• Outros Autores: Nishiya, Anna Shoko [UNIFESP], Sabino, Ester Cerdeira [UNIFESP], Teixeira, P. S., Petrella, S., Milanelo, L., Vieira, J. C. F. [UNIFESP], Diaz, Ricardo Sobhie [UNIFESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNIFESP
• Texto Completo: http://dx.doi.org/10.4067/S0717-95022008000200018; http://repositorio.unifesp.br/handle/11600/44057
• topic: Torque Teno Virus; Torque Teno Minivirus; Non-humans primates; Untranslated region; Nucleotide sequencing

Torque teno virus (TTV) is an infectious agent of worldwide distribution isolated by the first time as the agent of an acute post-transfusion hepatitis in a patient in Japan. It has been classified into a new floating genus called Anellovirus. Recent studies showed that TTV can also be identified in serum specimens obtained from domesticated farm animals and from non-human primates. To better understand the relationship between TTV and their hosts, a study to detect virus in the serum and whole blood of Brazilian non-human primates and in the plasm of chickens was performed by applying the PCR-UTR-A technique, followed by a genomic sequence and phylogenetic analysis. By nested-PCR-UTR, the DNA of TTV was detected in sera from 4 (5.3%) of 75 Cebus apella, 2 (40%) of 5 Alouata fusca, 1 (20%) of 5 Alouata caraya, 1 (5.2%) of 19 Callithrix penicilata, 1 (4%) of 25 Callithrix jacchus, 1 ( 20%) of 5 Saimiri sciureus and 1 (25%) of 4 Leontopithecus chrysomelas. Phylogenetic analysis revealed that sequences detected in 8 samples clustered with TTV sequences So-TTV2 (Saguinus oedipus) and At-TTV3 (Aotes Trivirgatus). Three sequences showed similarity with a human Torque Teno Minivirus (TLMV). TTV ORF2 DNA was detected in one sera sample and one whole blood sample of non-human primates and in one plasm sample of chicken. Phylogenetic analysis revealed that the sequences amplified by the ORF2 region show no difference between human, non-human primates and chicken. This is the first report of TTV in brazilian new world non-human primates and chicken.