High occurrence of shiga-like toxin-producing strains among diarrheagenic Escherichia coli isolated from raw beef products in Rio de Janeiro City, Brazil

Detalhes bibliográficos
Autor(a) principal: Cerqueira, Aloysio MF
Data de Publicação: 1997
Outros Autores: Tibana, Anita, Guth, Beatriz Ernestina Cabilio [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.4315/0362-028X-60.2.177
http://repositorio.unifesp.br/handle/11600/44366
Resumo: Raw beef samples (n=105) were examined for diarrheagenic Escherichia coli (DEC) using standard methods. The isolates obtained (n=1,066) were screened for Shiga-like toxins (SLT-I and SLT-II), cytolethal distending toxin (CLDT), enterotoxins (LT-I and STa), and classical enteropathogenic (EPEC) and enteroinvasive (EIEC) serogroups. Seventy-three (6.8%) DEC isolates representing 42 strains isolated from 34 (32.4%) beef samples were detected. SLT-producing E. coli (SLTEC) was the most frequent DEC category found and corresponded to 21 (50%) of the 42 DEC strains. Several serotypes were detected among the SLTEC and some of them have been found previously in animal and human isolates, but E. coli O157:H7 was not isolated. Other virulence markers found in DEC strains included enterotoxin production (38.1%), CLDT (7.1%), and EPEC serogroups (4.3%). This is the first report of CLDT-producing E. coli (CLDTEC) isolated from food samples in Brazil. Production of both SLT-I and LT-I was found in one E. coli isolate, and 3 beef samples harbored both SLTEC and ETEC strains. Although a high frequency of DEC groups was found in commercial beef samples in Rio de Janeiro City, Brazil, the significance of these strains as agents of human diarrhea remains to be established.
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spelling High occurrence of shiga-like toxin-producing strains among diarrheagenic Escherichia coli isolated from raw beef products in Rio de Janeiro City, Brazilbeef productsdiarrheagenic Escherichia coliShiga like toxinserotypesRaw beef samples (n=105) were examined for diarrheagenic Escherichia coli (DEC) using standard methods. The isolates obtained (n=1,066) were screened for Shiga-like toxins (SLT-I and SLT-II), cytolethal distending toxin (CLDT), enterotoxins (LT-I and STa), and classical enteropathogenic (EPEC) and enteroinvasive (EIEC) serogroups. Seventy-three (6.8%) DEC isolates representing 42 strains isolated from 34 (32.4%) beef samples were detected. SLT-producing E. coli (SLTEC) was the most frequent DEC category found and corresponded to 21 (50%) of the 42 DEC strains. Several serotypes were detected among the SLTEC and some of them have been found previously in animal and human isolates, but E. coli O157:H7 was not isolated. Other virulence markers found in DEC strains included enterotoxin production (38.1%), CLDT (7.1%), and EPEC serogroups (4.3%). This is the first report of CLDT-producing E. coli (CLDTEC) isolated from food samples in Brazil. Production of both SLT-I and LT-I was found in one E. coli isolate, and 3 beef samples harbored both SLTEC and ETEC strains. Although a high frequency of DEC groups was found in commercial beef samples in Rio de Janeiro City, Brazil, the significance of these strains as agents of human diarrhea remains to be established.FED UNIV RIO DE JANEIRO,INST MICROBIOL,RIO JANEIRO,BRAZILUNIV FED SAO PAULO,ESCOLA PAULISTA MED,DISCIPLINA MICROBIOL,SAO PAULO,BRAZILUNIV FED SAO PAULO,ESCOLA PAULISTA MED,DISCIPLINA MICROBIOL,SAO PAULO,BRAZILWeb of ScienceInt Assoc Milk Food Environmental Sanitarians, IncUniversidade Federal do Rio de Janeiro (UFRJ)Universidade Federal de São Paulo (UNIFESP)Cerqueira, Aloysio MFTibana, AnitaGuth, Beatriz Ernestina Cabilio [UNIFESP]2018-06-15T17:58:47Z2018-06-15T17:58:47Z1997-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion177-180http://dx.doi.org/10.4315/0362-028X-60.2.177Journal Of Food Protection. Des Moines: Int Assoc Milk Food Environmental Sanitarians, Inc, v. 60, n. 2, p. 177-180, 1997.10.4315/0362-028X-60.2.1770362-028Xhttp://repositorio.unifesp.br/handle/11600/44366WOS:A1997WJ83300015engJournal Of Food Protectioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-05-02T15:52:24Zoai:repositorio.unifesp.br/:11600/44366Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-05-02T15:52:24Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv High occurrence of shiga-like toxin-producing strains among diarrheagenic Escherichia coli isolated from raw beef products in Rio de Janeiro City, Brazil
title High occurrence of shiga-like toxin-producing strains among diarrheagenic Escherichia coli isolated from raw beef products in Rio de Janeiro City, Brazil
spellingShingle High occurrence of shiga-like toxin-producing strains among diarrheagenic Escherichia coli isolated from raw beef products in Rio de Janeiro City, Brazil
Cerqueira, Aloysio MF
beef products
diarrheagenic Escherichia coli
Shiga like toxin
serotypes
title_short High occurrence of shiga-like toxin-producing strains among diarrheagenic Escherichia coli isolated from raw beef products in Rio de Janeiro City, Brazil
title_full High occurrence of shiga-like toxin-producing strains among diarrheagenic Escherichia coli isolated from raw beef products in Rio de Janeiro City, Brazil
title_fullStr High occurrence of shiga-like toxin-producing strains among diarrheagenic Escherichia coli isolated from raw beef products in Rio de Janeiro City, Brazil
title_full_unstemmed High occurrence of shiga-like toxin-producing strains among diarrheagenic Escherichia coli isolated from raw beef products in Rio de Janeiro City, Brazil
title_sort High occurrence of shiga-like toxin-producing strains among diarrheagenic Escherichia coli isolated from raw beef products in Rio de Janeiro City, Brazil
author Cerqueira, Aloysio MF
author_facet Cerqueira, Aloysio MF
Tibana, Anita
Guth, Beatriz Ernestina Cabilio [UNIFESP]
author_role author
author2 Tibana, Anita
Guth, Beatriz Ernestina Cabilio [UNIFESP]
author2_role author
author
dc.contributor.none.fl_str_mv Universidade Federal do Rio de Janeiro (UFRJ)
Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Cerqueira, Aloysio MF
Tibana, Anita
Guth, Beatriz Ernestina Cabilio [UNIFESP]
dc.subject.por.fl_str_mv beef products
diarrheagenic Escherichia coli
Shiga like toxin
serotypes
topic beef products
diarrheagenic Escherichia coli
Shiga like toxin
serotypes
description Raw beef samples (n=105) were examined for diarrheagenic Escherichia coli (DEC) using standard methods. The isolates obtained (n=1,066) were screened for Shiga-like toxins (SLT-I and SLT-II), cytolethal distending toxin (CLDT), enterotoxins (LT-I and STa), and classical enteropathogenic (EPEC) and enteroinvasive (EIEC) serogroups. Seventy-three (6.8%) DEC isolates representing 42 strains isolated from 34 (32.4%) beef samples were detected. SLT-producing E. coli (SLTEC) was the most frequent DEC category found and corresponded to 21 (50%) of the 42 DEC strains. Several serotypes were detected among the SLTEC and some of them have been found previously in animal and human isolates, but E. coli O157:H7 was not isolated. Other virulence markers found in DEC strains included enterotoxin production (38.1%), CLDT (7.1%), and EPEC serogroups (4.3%). This is the first report of CLDT-producing E. coli (CLDTEC) isolated from food samples in Brazil. Production of both SLT-I and LT-I was found in one E. coli isolate, and 3 beef samples harbored both SLTEC and ETEC strains. Although a high frequency of DEC groups was found in commercial beef samples in Rio de Janeiro City, Brazil, the significance of these strains as agents of human diarrhea remains to be established.
publishDate 1997
dc.date.none.fl_str_mv 1997-02-01
2018-06-15T17:58:47Z
2018-06-15T17:58:47Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.4315/0362-028X-60.2.177
Journal Of Food Protection. Des Moines: Int Assoc Milk Food Environmental Sanitarians, Inc, v. 60, n. 2, p. 177-180, 1997.
10.4315/0362-028X-60.2.177
0362-028X
http://repositorio.unifesp.br/handle/11600/44366
WOS:A1997WJ83300015
url http://dx.doi.org/10.4315/0362-028X-60.2.177
http://repositorio.unifesp.br/handle/11600/44366
identifier_str_mv Journal Of Food Protection. Des Moines: Int Assoc Milk Food Environmental Sanitarians, Inc, v. 60, n. 2, p. 177-180, 1997.
10.4315/0362-028X-60.2.177
0362-028X
WOS:A1997WJ83300015
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal Of Food Protection
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 177-180
dc.publisher.none.fl_str_mv Int Assoc Milk Food Environmental Sanitarians, Inc
publisher.none.fl_str_mv Int Assoc Milk Food Environmental Sanitarians, Inc
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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