High occurrence of shiga-like toxin-producing strains among diarrheagenic Escherichia coli isolated from raw beef products in Rio de Janeiro City, Brazil
Autor(a) principal: | |
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Data de Publicação: | 1997 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://dx.doi.org/10.4315/0362-028X-60.2.177 http://repositorio.unifesp.br/handle/11600/44366 |
Resumo: | Raw beef samples (n=105) were examined for diarrheagenic Escherichia coli (DEC) using standard methods. The isolates obtained (n=1,066) were screened for Shiga-like toxins (SLT-I and SLT-II), cytolethal distending toxin (CLDT), enterotoxins (LT-I and STa), and classical enteropathogenic (EPEC) and enteroinvasive (EIEC) serogroups. Seventy-three (6.8%) DEC isolates representing 42 strains isolated from 34 (32.4%) beef samples were detected. SLT-producing E. coli (SLTEC) was the most frequent DEC category found and corresponded to 21 (50%) of the 42 DEC strains. Several serotypes were detected among the SLTEC and some of them have been found previously in animal and human isolates, but E. coli O157:H7 was not isolated. Other virulence markers found in DEC strains included enterotoxin production (38.1%), CLDT (7.1%), and EPEC serogroups (4.3%). This is the first report of CLDT-producing E. coli (CLDTEC) isolated from food samples in Brazil. Production of both SLT-I and LT-I was found in one E. coli isolate, and 3 beef samples harbored both SLTEC and ETEC strains. Although a high frequency of DEC groups was found in commercial beef samples in Rio de Janeiro City, Brazil, the significance of these strains as agents of human diarrhea remains to be established. |
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High occurrence of shiga-like toxin-producing strains among diarrheagenic Escherichia coli isolated from raw beef products in Rio de Janeiro City, Brazilbeef productsdiarrheagenic Escherichia coliShiga like toxinserotypesRaw beef samples (n=105) were examined for diarrheagenic Escherichia coli (DEC) using standard methods. The isolates obtained (n=1,066) were screened for Shiga-like toxins (SLT-I and SLT-II), cytolethal distending toxin (CLDT), enterotoxins (LT-I and STa), and classical enteropathogenic (EPEC) and enteroinvasive (EIEC) serogroups. Seventy-three (6.8%) DEC isolates representing 42 strains isolated from 34 (32.4%) beef samples were detected. SLT-producing E. coli (SLTEC) was the most frequent DEC category found and corresponded to 21 (50%) of the 42 DEC strains. Several serotypes were detected among the SLTEC and some of them have been found previously in animal and human isolates, but E. coli O157:H7 was not isolated. Other virulence markers found in DEC strains included enterotoxin production (38.1%), CLDT (7.1%), and EPEC serogroups (4.3%). This is the first report of CLDT-producing E. coli (CLDTEC) isolated from food samples in Brazil. Production of both SLT-I and LT-I was found in one E. coli isolate, and 3 beef samples harbored both SLTEC and ETEC strains. Although a high frequency of DEC groups was found in commercial beef samples in Rio de Janeiro City, Brazil, the significance of these strains as agents of human diarrhea remains to be established.FED UNIV RIO DE JANEIRO,INST MICROBIOL,RIO JANEIRO,BRAZILUNIV FED SAO PAULO,ESCOLA PAULISTA MED,DISCIPLINA MICROBIOL,SAO PAULO,BRAZILUNIV FED SAO PAULO,ESCOLA PAULISTA MED,DISCIPLINA MICROBIOL,SAO PAULO,BRAZILWeb of ScienceInt Assoc Milk Food Environmental Sanitarians, IncUniversidade Federal do Rio de Janeiro (UFRJ)Universidade Federal de São Paulo (UNIFESP)Cerqueira, Aloysio MFTibana, AnitaGuth, Beatriz Ernestina Cabilio [UNIFESP]2018-06-15T17:58:47Z2018-06-15T17:58:47Z1997-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion177-180http://dx.doi.org/10.4315/0362-028X-60.2.177Journal Of Food Protection. Des Moines: Int Assoc Milk Food Environmental Sanitarians, Inc, v. 60, n. 2, p. 177-180, 1997.10.4315/0362-028X-60.2.1770362-028Xhttp://repositorio.unifesp.br/handle/11600/44366WOS:A1997WJ83300015engJournal Of Food Protectioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-05-02T15:52:24Zoai:repositorio.unifesp.br/:11600/44366Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-05-02T15:52:24Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
High occurrence of shiga-like toxin-producing strains among diarrheagenic Escherichia coli isolated from raw beef products in Rio de Janeiro City, Brazil |
title |
High occurrence of shiga-like toxin-producing strains among diarrheagenic Escherichia coli isolated from raw beef products in Rio de Janeiro City, Brazil |
spellingShingle |
High occurrence of shiga-like toxin-producing strains among diarrheagenic Escherichia coli isolated from raw beef products in Rio de Janeiro City, Brazil Cerqueira, Aloysio MF beef products diarrheagenic Escherichia coli Shiga like toxin serotypes |
title_short |
High occurrence of shiga-like toxin-producing strains among diarrheagenic Escherichia coli isolated from raw beef products in Rio de Janeiro City, Brazil |
title_full |
High occurrence of shiga-like toxin-producing strains among diarrheagenic Escherichia coli isolated from raw beef products in Rio de Janeiro City, Brazil |
title_fullStr |
High occurrence of shiga-like toxin-producing strains among diarrheagenic Escherichia coli isolated from raw beef products in Rio de Janeiro City, Brazil |
title_full_unstemmed |
High occurrence of shiga-like toxin-producing strains among diarrheagenic Escherichia coli isolated from raw beef products in Rio de Janeiro City, Brazil |
title_sort |
High occurrence of shiga-like toxin-producing strains among diarrheagenic Escherichia coli isolated from raw beef products in Rio de Janeiro City, Brazil |
author |
Cerqueira, Aloysio MF |
author_facet |
Cerqueira, Aloysio MF Tibana, Anita Guth, Beatriz Ernestina Cabilio [UNIFESP] |
author_role |
author |
author2 |
Tibana, Anita Guth, Beatriz Ernestina Cabilio [UNIFESP] |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade Federal do Rio de Janeiro (UFRJ) Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Cerqueira, Aloysio MF Tibana, Anita Guth, Beatriz Ernestina Cabilio [UNIFESP] |
dc.subject.por.fl_str_mv |
beef products diarrheagenic Escherichia coli Shiga like toxin serotypes |
topic |
beef products diarrheagenic Escherichia coli Shiga like toxin serotypes |
description |
Raw beef samples (n=105) were examined for diarrheagenic Escherichia coli (DEC) using standard methods. The isolates obtained (n=1,066) were screened for Shiga-like toxins (SLT-I and SLT-II), cytolethal distending toxin (CLDT), enterotoxins (LT-I and STa), and classical enteropathogenic (EPEC) and enteroinvasive (EIEC) serogroups. Seventy-three (6.8%) DEC isolates representing 42 strains isolated from 34 (32.4%) beef samples were detected. SLT-producing E. coli (SLTEC) was the most frequent DEC category found and corresponded to 21 (50%) of the 42 DEC strains. Several serotypes were detected among the SLTEC and some of them have been found previously in animal and human isolates, but E. coli O157:H7 was not isolated. Other virulence markers found in DEC strains included enterotoxin production (38.1%), CLDT (7.1%), and EPEC serogroups (4.3%). This is the first report of CLDT-producing E. coli (CLDTEC) isolated from food samples in Brazil. Production of both SLT-I and LT-I was found in one E. coli isolate, and 3 beef samples harbored both SLTEC and ETEC strains. Although a high frequency of DEC groups was found in commercial beef samples in Rio de Janeiro City, Brazil, the significance of these strains as agents of human diarrhea remains to be established. |
publishDate |
1997 |
dc.date.none.fl_str_mv |
1997-02-01 2018-06-15T17:58:47Z 2018-06-15T17:58:47Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.4315/0362-028X-60.2.177 Journal Of Food Protection. Des Moines: Int Assoc Milk Food Environmental Sanitarians, Inc, v. 60, n. 2, p. 177-180, 1997. 10.4315/0362-028X-60.2.177 0362-028X http://repositorio.unifesp.br/handle/11600/44366 WOS:A1997WJ83300015 |
url |
http://dx.doi.org/10.4315/0362-028X-60.2.177 http://repositorio.unifesp.br/handle/11600/44366 |
identifier_str_mv |
Journal Of Food Protection. Des Moines: Int Assoc Milk Food Environmental Sanitarians, Inc, v. 60, n. 2, p. 177-180, 1997. 10.4315/0362-028X-60.2.177 0362-028X WOS:A1997WJ83300015 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal Of Food Protection |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
177-180 |
dc.publisher.none.fl_str_mv |
Int Assoc Milk Food Environmental Sanitarians, Inc |
publisher.none.fl_str_mv |
Int Assoc Milk Food Environmental Sanitarians, Inc |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
_version_ |
1814268313992167424 |