Hamster (Mesocricetus auratus) cheek pouch as an experimental model to investigate human skin and keloid heterologous graft

Detalhes bibliográficos
Autor(a) principal: Hochman, Bernardo [UNIFESP]
Data de Publicação: 2004
Outros Autores: Ferreira, Lydia Masako [UNIFESP], Bôas, Flaviane Cássia Vilas [UNIFESP], Mariano, Mario [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
dARK ID: ark:/48912/0013000009090
DOI: 10.1590/S0102-86502004000700014
Texto Completo: http://dx.doi.org/10.1590/S0102-86502004000700014
http://repositorio.unifesp.br/handle/11600/2306
Resumo: To describe the integration process of grafts of total human skin and keloid in hamster (Mesocricetus auratus) cheek pouch, whose sub-epithelium is naturally an Immunologically Privileged Site. Fragments of human normal skin and keloid from the breast region of mulatto female patients were transplanted into the cheek pouch subepithelium in situ. Surgical procedure and grafted pouches for microscopic exam at several time points of the transplantation were standardized. The integration of grafted fragments of human skin and keloid was seen in late periods (84 days) since the microscopic assessment showed the presence of blood vases within the conjunctive tissue of grafted fragments. It was also possible to see among the grafted fragments the epithelium, the appearing of early cellular infiltrated, epithelial secretion of keratin, the presence of melanocytes, and delayed changes on the aspect of collagen fibers of conjunctive tissue. Pooled results allow to define hamster cheek pouch sub-epithelium as an experimental model to investigating heterologous graft physiology of human total skin and keloid with epithelium.
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spelling Hamster (Mesocricetus auratus) cheek pouch as an experimental model to investigate human skin and keloid heterologous graftBolsa jugal no hamster (Mesocricetus auratus) como modelo experimental de investigação de enxertos heterólogos de pele humana e quelóideMesocricetusTransplantation, heterologousSkin transplantationKeloidMesocricetusTransplante heterólogoTransplante de peleQuelóideTo describe the integration process of grafts of total human skin and keloid in hamster (Mesocricetus auratus) cheek pouch, whose sub-epithelium is naturally an Immunologically Privileged Site. Fragments of human normal skin and keloid from the breast region of mulatto female patients were transplanted into the cheek pouch subepithelium in situ. Surgical procedure and grafted pouches for microscopic exam at several time points of the transplantation were standardized. The integration of grafted fragments of human skin and keloid was seen in late periods (84 days) since the microscopic assessment showed the presence of blood vases within the conjunctive tissue of grafted fragments. It was also possible to see among the grafted fragments the epithelium, the appearing of early cellular infiltrated, epithelial secretion of keratin, the presence of melanocytes, and delayed changes on the aspect of collagen fibers of conjunctive tissue. Pooled results allow to define hamster cheek pouch sub-epithelium as an experimental model to investigating heterologous graft physiology of human total skin and keloid with epithelium.Descrever a integração dos enxertos de pele total humana e quelóide na bolsa jugal do hamster (Mesocricetus auratus), cujo subepitélio é, naturalmente, um Local de Privilégio Imunológico. Foram transplantados fragmentos, de pele humana normal e de quelóide, obtidos da região mamária de pacientes pardas, no subepitélio da bolsa jugal in situ. O procedimento operatório, e de preparo das bolsas enxertadas para exame microscópico em vários períodos de transplante, foi padronizado. Verificou-se a integração dos fragmentos enxertados de pele humana e de quelóide em períodos tardios (84 dias), uma vez que a avaliação microscópica revelou a presença de vasos sangüíneos no tecido conjuntivo dos fragmentos enxertados. Foi também possível observar, nos fragmentos enxertados, o epitélio, o aparecimento de infiltrado celular incipiente, secreção epitelial de queratina, presença de melanócitos e alterações tardias no aspecto das fibras colágenas do tecido conjuntivo. Os resultados, em conjunto, permitem estabelecer o subepitélio da bolsa jugal do hamster como modelo experimental para investigação da fisiologia de tranplantes heterólogos de pele total humana e quelóide com epitélio.UNIFESP-EPMSão Paulo University Biomedical Sciences Institute Immunology DepartmentUSP Veterinary Medical and Zootechnical School Pathology DepartmentUNIFESP-EPM Immunology DepartmentUNIFESP, EPM, Immunology DepartmentSciELOSociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaUniversidade Federal de São Paulo (UNIFESP)São Paulo University Biomedical Sciences Institute Immunology DepartmentUniversidade de São Paulo (USP)Hochman, Bernardo [UNIFESP]Ferreira, Lydia Masako [UNIFESP]Bôas, Flaviane Cássia Vilas [UNIFESP]Mariano, Mario [UNIFESP]2015-06-14T13:31:21Z2015-06-14T13:31:21Z2004-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion79-88application/pdfhttp://dx.doi.org/10.1590/S0102-86502004000700014Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 19, p. 79-88, 2004.10.1590/S0102-86502004000700014S0102-86502004000700014.pdf0102-8650S0102-86502004000700014http://repositorio.unifesp.br/handle/11600/2306ark:/48912/0013000009090engActa Cirurgica Brasileirainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-04T13:06:07Zoai:repositorio.unifesp.br/:11600/2306Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-12-11T20:06:15.770922Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Hamster (Mesocricetus auratus) cheek pouch as an experimental model to investigate human skin and keloid heterologous graft
Bolsa jugal no hamster (Mesocricetus auratus) como modelo experimental de investigação de enxertos heterólogos de pele humana e quelóide
title Hamster (Mesocricetus auratus) cheek pouch as an experimental model to investigate human skin and keloid heterologous graft
spellingShingle Hamster (Mesocricetus auratus) cheek pouch as an experimental model to investigate human skin and keloid heterologous graft
Hamster (Mesocricetus auratus) cheek pouch as an experimental model to investigate human skin and keloid heterologous graft
Hochman, Bernardo [UNIFESP]
Mesocricetus
Transplantation, heterologous
Skin transplantation
Keloid
Mesocricetus
Transplante heterólogo
Transplante de pele
Quelóide
Hochman, Bernardo [UNIFESP]
Mesocricetus
Transplantation, heterologous
Skin transplantation
Keloid
Mesocricetus
Transplante heterólogo
Transplante de pele
Quelóide
title_short Hamster (Mesocricetus auratus) cheek pouch as an experimental model to investigate human skin and keloid heterologous graft
title_full Hamster (Mesocricetus auratus) cheek pouch as an experimental model to investigate human skin and keloid heterologous graft
title_fullStr Hamster (Mesocricetus auratus) cheek pouch as an experimental model to investigate human skin and keloid heterologous graft
Hamster (Mesocricetus auratus) cheek pouch as an experimental model to investigate human skin and keloid heterologous graft
title_full_unstemmed Hamster (Mesocricetus auratus) cheek pouch as an experimental model to investigate human skin and keloid heterologous graft
Hamster (Mesocricetus auratus) cheek pouch as an experimental model to investigate human skin and keloid heterologous graft
title_sort Hamster (Mesocricetus auratus) cheek pouch as an experimental model to investigate human skin and keloid heterologous graft
author Hochman, Bernardo [UNIFESP]
author_facet Hochman, Bernardo [UNIFESP]
Hochman, Bernardo [UNIFESP]
Ferreira, Lydia Masako [UNIFESP]
Bôas, Flaviane Cássia Vilas [UNIFESP]
Mariano, Mario [UNIFESP]
Ferreira, Lydia Masako [UNIFESP]
Bôas, Flaviane Cássia Vilas [UNIFESP]
Mariano, Mario [UNIFESP]
author_role author
author2 Ferreira, Lydia Masako [UNIFESP]
Bôas, Flaviane Cássia Vilas [UNIFESP]
Mariano, Mario [UNIFESP]
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
São Paulo University Biomedical Sciences Institute Immunology Department
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Hochman, Bernardo [UNIFESP]
Ferreira, Lydia Masako [UNIFESP]
Bôas, Flaviane Cássia Vilas [UNIFESP]
Mariano, Mario [UNIFESP]
dc.subject.por.fl_str_mv Mesocricetus
Transplantation, heterologous
Skin transplantation
Keloid
Mesocricetus
Transplante heterólogo
Transplante de pele
Quelóide
topic Mesocricetus
Transplantation, heterologous
Skin transplantation
Keloid
Mesocricetus
Transplante heterólogo
Transplante de pele
Quelóide
description To describe the integration process of grafts of total human skin and keloid in hamster (Mesocricetus auratus) cheek pouch, whose sub-epithelium is naturally an Immunologically Privileged Site. Fragments of human normal skin and keloid from the breast region of mulatto female patients were transplanted into the cheek pouch subepithelium in situ. Surgical procedure and grafted pouches for microscopic exam at several time points of the transplantation were standardized. The integration of grafted fragments of human skin and keloid was seen in late periods (84 days) since the microscopic assessment showed the presence of blood vases within the conjunctive tissue of grafted fragments. It was also possible to see among the grafted fragments the epithelium, the appearing of early cellular infiltrated, epithelial secretion of keratin, the presence of melanocytes, and delayed changes on the aspect of collagen fibers of conjunctive tissue. Pooled results allow to define hamster cheek pouch sub-epithelium as an experimental model to investigating heterologous graft physiology of human total skin and keloid with epithelium.
publishDate 2004
dc.date.none.fl_str_mv 2004-12-01
2015-06-14T13:31:21Z
2015-06-14T13:31:21Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0102-86502004000700014
Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 19, p. 79-88, 2004.
10.1590/S0102-86502004000700014
S0102-86502004000700014.pdf
0102-8650
S0102-86502004000700014
http://repositorio.unifesp.br/handle/11600/2306
dc.identifier.dark.fl_str_mv ark:/48912/0013000009090
url http://dx.doi.org/10.1590/S0102-86502004000700014
http://repositorio.unifesp.br/handle/11600/2306
identifier_str_mv Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 19, p. 79-88, 2004.
10.1590/S0102-86502004000700014
S0102-86502004000700014.pdf
0102-8650
S0102-86502004000700014
ark:/48912/0013000009090
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Acta Cirurgica Brasileira
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 79-88
application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
_version_ 1822183947423973376
dc.identifier.doi.none.fl_str_mv 10.1590/S0102-86502004000700014

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