Diffuse and enteroaggregative patterns of adherence of Escherichia coli isolated from stools of children in northeastern Brazil
Autor(a) principal: | |
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Data de Publicação: | 2001 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://dx.doi.org/10.1590/S1517-83822001000400012 http://repositorio.unifesp.br/handle/11600/1298 |
Resumo: | Childhood diarrheal diseases remain highly endemic in northeastern Brazil. The attributable fraction of all diarrheal diseases among children less than 2 years of age due to Escherichia coli was examined in a 2-year prospective study in two large urban centers of Brazil. Between May 1997 and June 1999, fecal E. coli isolates from 237 children with diarrhea (217 acute and 20 persistent cases) and 231 children without diarrhea (controls) attending two hospitals in Northeast Brazil were tested for their pattern of adherence to HEp-2 cells and for colony hybridization with DNA probes specific for the six pathotypes of diarrheagenic E. coli. Enteroinvasive E. coli, enterotoxigenic E. coli and enterohemorrhagic E. coli were not isolated from any children. Diffusely adherent E. coli (DAEC) and enteroaggregative E. coli (EAEC) were the most frequent isolates with similar frequencies from children with or without diarrhea. Atypical EPEC (EAF-negative) strains were isolated with similiar frequency from both cases (5.5%) and controls (5.6%). Enteropathogenic E. coli (typical EPEC) strains, characterized by localized adherence pattern of adherence, hybridization with the EAF probe, and belonging to the classical O serogroups, were significantly associated with diarrhea (P = 0.03). These E. coli strains associated with diarrhea accounted for 9% of all children with diarrhea. Collectively, in Northeast Brazil, E. coli strains comprise a small proportion of severe diarrhea prevalence in children. |
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Diffuse and enteroaggregative patterns of adherence of Escherichia coli isolated from stools of children in northeastern BrazilIsolamento dos padrões de adesão difusa e agregativa de Escherichia coli nas fezes de crianças da região nordeste do BrasilEscherichia colidiffusely adherent E. colienteroaggregative E. coliadherence patternschildhood diarrheaEscherichia coliE. coli que adere difusamenteE. coli enteroagregativapadrões de adesãodiarréia infantilChildhood diarrheal diseases remain highly endemic in northeastern Brazil. The attributable fraction of all diarrheal diseases among children less than 2 years of age due to Escherichia coli was examined in a 2-year prospective study in two large urban centers of Brazil. Between May 1997 and June 1999, fecal E. coli isolates from 237 children with diarrhea (217 acute and 20 persistent cases) and 231 children without diarrhea (controls) attending two hospitals in Northeast Brazil were tested for their pattern of adherence to HEp-2 cells and for colony hybridization with DNA probes specific for the six pathotypes of diarrheagenic E. coli. Enteroinvasive E. coli, enterotoxigenic E. coli and enterohemorrhagic E. coli were not isolated from any children. Diffusely adherent E. coli (DAEC) and enteroaggregative E. coli (EAEC) were the most frequent isolates with similar frequencies from children with or without diarrhea. Atypical EPEC (EAF-negative) strains were isolated with similiar frequency from both cases (5.5%) and controls (5.6%). Enteropathogenic E. coli (typical EPEC) strains, characterized by localized adherence pattern of adherence, hybridization with the EAF probe, and belonging to the classical O serogroups, were significantly associated with diarrhea (P = 0.03). These E. coli strains associated with diarrhea accounted for 9% of all children with diarrhea. Collectively, in Northeast Brazil, E. coli strains comprise a small proportion of severe diarrhea prevalence in children.Na região nordeste do Brasil a doença diarréica na infância continua sendo altamente endêmica. Com o objetivo de determinar a prevalência das diferentes categorias de E. coli diarreiogênica foi realizado um estudo prospectivo de dois anos em dois grandes centros urbanos dessa região. Entre maio de 1997 a junho de 1999, foram examinadas amostras de Escherichia coli isoladas de 237 fezes de crianças com diarréia (217 aguda e 20 persistente casos) e 231 de crianças sem diarréia (controles) atendidas em dois hospitais na região nordeste do Brasil quanto a adesão a células HEp-2 e hibridização com sondas genéticas especîficas para as seis categorias de E. coli diarreiogênica. E. coli que adere difusamente (DAEC) e E. coli enteroagregativa (EAEC) foram as categorias mais frequentemente isoladas tanto em casos como em controles. E. coli enteropatogênica atípica (EPEC) foi isolada tanto em casos (5.5%) como em controles (5.6%). Amostras de E. coli enteropatogênica (EPEC típica), caracterizadas pelo padrão de adesão localizada, hibridização positiva com a sonda EAF e pertencentes aos sorogrupos clássicos foram mais frequentes em casos do que em controles, significantemente relacionadas com diarréia (P = 0.03). Essas amostras constituíram o terceiro patógeno, depois de rotavirus e Shigella spp, com predomínio significantemente maior entre os casos. Concluindo, na região nordeste do Brasil as EPEC respondem por 9% dos casos de diarréia grave em crianças menores de dois anos.Universidade Federal de São Paulo (UNIFESP) Departamento de Microbiologia, Imunologia e ParasitologiaUniversidade Federal de São Paulo (UNIFESP) Disciplina de Gastroenterologia PediátricaHospital Universitário Materno-InfantilUniversidade Federal do Rio Grande do Norte Departamento de PediatriaSchool of Public HealthUNIFESP, Depto. de Microbiologia, Imunologia e ParasitologiaUNIFESP, Disciplina de Gastroenterologia PediátricaSciELOFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Sociedade Brasileira de MicrobiologiaUniversidade Federal de São Paulo (UNIFESP)Hospital Universitário Materno-InfantilUniversidade Federal do Rio Grande do Norte Departamento de PediatriaSchool of Public HealthScaletsky, Isabel Cristina Affonso [UNIFESP]Fabbricotti, Sandra Hilde [UNIFESP]Carvalho, Rozane de Lima Bigelli [UNIFESP]Nunes, Claudia ReginaMaranhão, Helcio de SousaMorais, Mauro Batista de [UNIFESP]Riley, LeeFagundes-Neto, Ulysses [UNIFESP]2015-06-14T13:29:33Z2015-06-14T13:29:33Z2001-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion313-319application/pdfhttp://dx.doi.org/10.1590/S1517-83822001000400012Brazilian Journal of Microbiology. Sociedade Brasileira de Microbiologia, v. 32, n. 4, p. 313-319, 2001.10.1590/S1517-83822001000400012S1517-83822001000400012.pdf1517-8382S1517-83822001000400012http://repositorio.unifesp.br/handle/11600/1298WOS:000176404800012engBrazilian Journal of Microbiologyinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-07-30T01:52:21Zoai:repositorio.unifesp.br/:11600/1298Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-07-30T01:52:21Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
Diffuse and enteroaggregative patterns of adherence of Escherichia coli isolated from stools of children in northeastern Brazil Isolamento dos padrões de adesão difusa e agregativa de Escherichia coli nas fezes de crianças da região nordeste do Brasil |
title |
Diffuse and enteroaggregative patterns of adherence of Escherichia coli isolated from stools of children in northeastern Brazil |
spellingShingle |
Diffuse and enteroaggregative patterns of adherence of Escherichia coli isolated from stools of children in northeastern Brazil Scaletsky, Isabel Cristina Affonso [UNIFESP] Escherichia coli diffusely adherent E. coli enteroaggregative E. coli adherence patterns childhood diarrhea Escherichia coli E. coli que adere difusamente E. coli enteroagregativa padrões de adesão diarréia infantil |
title_short |
Diffuse and enteroaggregative patterns of adherence of Escherichia coli isolated from stools of children in northeastern Brazil |
title_full |
Diffuse and enteroaggregative patterns of adherence of Escherichia coli isolated from stools of children in northeastern Brazil |
title_fullStr |
Diffuse and enteroaggregative patterns of adherence of Escherichia coli isolated from stools of children in northeastern Brazil |
title_full_unstemmed |
Diffuse and enteroaggregative patterns of adherence of Escherichia coli isolated from stools of children in northeastern Brazil |
title_sort |
Diffuse and enteroaggregative patterns of adherence of Escherichia coli isolated from stools of children in northeastern Brazil |
author |
Scaletsky, Isabel Cristina Affonso [UNIFESP] |
author_facet |
Scaletsky, Isabel Cristina Affonso [UNIFESP] Fabbricotti, Sandra Hilde [UNIFESP] Carvalho, Rozane de Lima Bigelli [UNIFESP] Nunes, Claudia Regina Maranhão, Helcio de Sousa Morais, Mauro Batista de [UNIFESP] Riley, Lee Fagundes-Neto, Ulysses [UNIFESP] |
author_role |
author |
author2 |
Fabbricotti, Sandra Hilde [UNIFESP] Carvalho, Rozane de Lima Bigelli [UNIFESP] Nunes, Claudia Regina Maranhão, Helcio de Sousa Morais, Mauro Batista de [UNIFESP] Riley, Lee Fagundes-Neto, Ulysses [UNIFESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Hospital Universitário Materno-Infantil Universidade Federal do Rio Grande do Norte Departamento de Pediatria School of Public Health |
dc.contributor.author.fl_str_mv |
Scaletsky, Isabel Cristina Affonso [UNIFESP] Fabbricotti, Sandra Hilde [UNIFESP] Carvalho, Rozane de Lima Bigelli [UNIFESP] Nunes, Claudia Regina Maranhão, Helcio de Sousa Morais, Mauro Batista de [UNIFESP] Riley, Lee Fagundes-Neto, Ulysses [UNIFESP] |
dc.subject.por.fl_str_mv |
Escherichia coli diffusely adherent E. coli enteroaggregative E. coli adherence patterns childhood diarrhea Escherichia coli E. coli que adere difusamente E. coli enteroagregativa padrões de adesão diarréia infantil |
topic |
Escherichia coli diffusely adherent E. coli enteroaggregative E. coli adherence patterns childhood diarrhea Escherichia coli E. coli que adere difusamente E. coli enteroagregativa padrões de adesão diarréia infantil |
description |
Childhood diarrheal diseases remain highly endemic in northeastern Brazil. The attributable fraction of all diarrheal diseases among children less than 2 years of age due to Escherichia coli was examined in a 2-year prospective study in two large urban centers of Brazil. Between May 1997 and June 1999, fecal E. coli isolates from 237 children with diarrhea (217 acute and 20 persistent cases) and 231 children without diarrhea (controls) attending two hospitals in Northeast Brazil were tested for their pattern of adherence to HEp-2 cells and for colony hybridization with DNA probes specific for the six pathotypes of diarrheagenic E. coli. Enteroinvasive E. coli, enterotoxigenic E. coli and enterohemorrhagic E. coli were not isolated from any children. Diffusely adherent E. coli (DAEC) and enteroaggregative E. coli (EAEC) were the most frequent isolates with similar frequencies from children with or without diarrhea. Atypical EPEC (EAF-negative) strains were isolated with similiar frequency from both cases (5.5%) and controls (5.6%). Enteropathogenic E. coli (typical EPEC) strains, characterized by localized adherence pattern of adherence, hybridization with the EAF probe, and belonging to the classical O serogroups, were significantly associated with diarrhea (P = 0.03). These E. coli strains associated with diarrhea accounted for 9% of all children with diarrhea. Collectively, in Northeast Brazil, E. coli strains comprise a small proportion of severe diarrhea prevalence in children. |
publishDate |
2001 |
dc.date.none.fl_str_mv |
2001-12-01 2015-06-14T13:29:33Z 2015-06-14T13:29:33Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1517-83822001000400012 Brazilian Journal of Microbiology. Sociedade Brasileira de Microbiologia, v. 32, n. 4, p. 313-319, 2001. 10.1590/S1517-83822001000400012 S1517-83822001000400012.pdf 1517-8382 S1517-83822001000400012 http://repositorio.unifesp.br/handle/11600/1298 WOS:000176404800012 |
url |
http://dx.doi.org/10.1590/S1517-83822001000400012 http://repositorio.unifesp.br/handle/11600/1298 |
identifier_str_mv |
Brazilian Journal of Microbiology. Sociedade Brasileira de Microbiologia, v. 32, n. 4, p. 313-319, 2001. 10.1590/S1517-83822001000400012 S1517-83822001000400012.pdf 1517-8382 S1517-83822001000400012 WOS:000176404800012 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Journal of Microbiology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
313-319 application/pdf |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
_version_ |
1814268318172839936 |