Diffuse and enteroaggregative patterns of adherence of Escherichia coli isolated from stools of children in northeastern Brazil

Detalhes bibliográficos
Autor(a) principal: Scaletsky, Isabel Cristina Affonso [UNIFESP]
Data de Publicação: 2001
Outros Autores: Fabbricotti, Sandra Hilde [UNIFESP], Carvalho, Rozane de Lima Bigelli [UNIFESP], Nunes, Claudia Regina, Maranhão, Helcio de Sousa, Morais, Mauro Batista de [UNIFESP], Riley, Lee, Fagundes-Neto, Ulysses [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1590/S1517-83822001000400012
http://repositorio.unifesp.br/handle/11600/1298
Resumo: Childhood diarrheal diseases remain highly endemic in northeastern Brazil. The attributable fraction of all diarrheal diseases among children less than 2 years of age due to Escherichia coli was examined in a 2-year prospective study in two large urban centers of Brazil. Between May 1997 and June 1999, fecal E. coli isolates from 237 children with diarrhea (217 acute and 20 persistent cases) and 231 children without diarrhea (controls) attending two hospitals in Northeast Brazil were tested for their pattern of adherence to HEp-2 cells and for colony hybridization with DNA probes specific for the six pathotypes of diarrheagenic E. coli. Enteroinvasive E. coli, enterotoxigenic E. coli and enterohemorrhagic E. coli were not isolated from any children. Diffusely adherent E. coli (DAEC) and enteroaggregative E. coli (EAEC) were the most frequent isolates with similar frequencies from children with or without diarrhea. Atypical EPEC (EAF-negative) strains were isolated with similiar frequency from both cases (5.5%) and controls (5.6%). Enteropathogenic E. coli (typical EPEC) strains, characterized by localized adherence pattern of adherence, hybridization with the EAF probe, and belonging to the classical O serogroups, were significantly associated with diarrhea (P = 0.03). These E. coli strains associated with diarrhea accounted for 9% of all children with diarrhea. Collectively, in Northeast Brazil, E. coli strains comprise a small proportion of severe diarrhea prevalence in children.
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spelling Diffuse and enteroaggregative patterns of adherence of Escherichia coli isolated from stools of children in northeastern BrazilIsolamento dos padrões de adesão difusa e agregativa de Escherichia coli nas fezes de crianças da região nordeste do BrasilEscherichia colidiffusely adherent E. colienteroaggregative E. coliadherence patternschildhood diarrheaEscherichia coliE. coli que adere difusamenteE. coli enteroagregativapadrões de adesãodiarréia infantilChildhood diarrheal diseases remain highly endemic in northeastern Brazil. The attributable fraction of all diarrheal diseases among children less than 2 years of age due to Escherichia coli was examined in a 2-year prospective study in two large urban centers of Brazil. Between May 1997 and June 1999, fecal E. coli isolates from 237 children with diarrhea (217 acute and 20 persistent cases) and 231 children without diarrhea (controls) attending two hospitals in Northeast Brazil were tested for their pattern of adherence to HEp-2 cells and for colony hybridization with DNA probes specific for the six pathotypes of diarrheagenic E. coli. Enteroinvasive E. coli, enterotoxigenic E. coli and enterohemorrhagic E. coli were not isolated from any children. Diffusely adherent E. coli (DAEC) and enteroaggregative E. coli (EAEC) were the most frequent isolates with similar frequencies from children with or without diarrhea. Atypical EPEC (EAF-negative) strains were isolated with similiar frequency from both cases (5.5%) and controls (5.6%). Enteropathogenic E. coli (typical EPEC) strains, characterized by localized adherence pattern of adherence, hybridization with the EAF probe, and belonging to the classical O serogroups, were significantly associated with diarrhea (P = 0.03). These E. coli strains associated with diarrhea accounted for 9% of all children with diarrhea. Collectively, in Northeast Brazil, E. coli strains comprise a small proportion of severe diarrhea prevalence in children.Na região nordeste do Brasil a doença diarréica na infância continua sendo altamente endêmica. Com o objetivo de determinar a prevalência das diferentes categorias de E. coli diarreiogênica foi realizado um estudo prospectivo de dois anos em dois grandes centros urbanos dessa região. Entre maio de 1997 a junho de 1999, foram examinadas amostras de Escherichia coli isoladas de 237 fezes de crianças com diarréia (217 aguda e 20 persistente casos) e 231 de crianças sem diarréia (controles) atendidas em dois hospitais na região nordeste do Brasil quanto a adesão a células HEp-2 e hibridização com sondas genéticas especîficas para as seis categorias de E. coli diarreiogênica. E. coli que adere difusamente (DAEC) e E. coli enteroagregativa (EAEC) foram as categorias mais frequentemente isoladas tanto em casos como em controles. E. coli enteropatogênica atípica (EPEC) foi isolada tanto em casos (5.5%) como em controles (5.6%). Amostras de E. coli enteropatogênica (EPEC típica), caracterizadas pelo padrão de adesão localizada, hibridização positiva com a sonda EAF e pertencentes aos sorogrupos clássicos foram mais frequentes em casos do que em controles, significantemente relacionadas com diarréia (P = 0.03). Essas amostras constituíram o terceiro patógeno, depois de rotavirus e Shigella spp, com predomínio significantemente maior entre os casos. Concluindo, na região nordeste do Brasil as EPEC respondem por 9% dos casos de diarréia grave em crianças menores de dois anos.Universidade Federal de São Paulo (UNIFESP) Departamento de Microbiologia, Imunologia e ParasitologiaUniversidade Federal de São Paulo (UNIFESP) Disciplina de Gastroenterologia PediátricaHospital Universitário Materno-InfantilUniversidade Federal do Rio Grande do Norte Departamento de PediatriaSchool of Public HealthUNIFESP, Depto. de Microbiologia, Imunologia e ParasitologiaUNIFESP, Disciplina de Gastroenterologia PediátricaSciELOFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Sociedade Brasileira de MicrobiologiaUniversidade Federal de São Paulo (UNIFESP)Hospital Universitário Materno-InfantilUniversidade Federal do Rio Grande do Norte Departamento de PediatriaSchool of Public HealthScaletsky, Isabel Cristina Affonso [UNIFESP]Fabbricotti, Sandra Hilde [UNIFESP]Carvalho, Rozane de Lima Bigelli [UNIFESP]Nunes, Claudia ReginaMaranhão, Helcio de SousaMorais, Mauro Batista de [UNIFESP]Riley, LeeFagundes-Neto, Ulysses [UNIFESP]2015-06-14T13:29:33Z2015-06-14T13:29:33Z2001-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion313-319application/pdfhttp://dx.doi.org/10.1590/S1517-83822001000400012Brazilian Journal of Microbiology. Sociedade Brasileira de Microbiologia, v. 32, n. 4, p. 313-319, 2001.10.1590/S1517-83822001000400012S1517-83822001000400012.pdf1517-8382S1517-83822001000400012http://repositorio.unifesp.br/handle/11600/1298WOS:000176404800012engBrazilian Journal of Microbiologyinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-07-30T01:52:21Zoai:repositorio.unifesp.br/:11600/1298Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-07-30T01:52:21Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Diffuse and enteroaggregative patterns of adherence of Escherichia coli isolated from stools of children in northeastern Brazil
Isolamento dos padrões de adesão difusa e agregativa de Escherichia coli nas fezes de crianças da região nordeste do Brasil
title Diffuse and enteroaggregative patterns of adherence of Escherichia coli isolated from stools of children in northeastern Brazil
spellingShingle Diffuse and enteroaggregative patterns of adherence of Escherichia coli isolated from stools of children in northeastern Brazil
Scaletsky, Isabel Cristina Affonso [UNIFESP]
Escherichia coli
diffusely adherent E. coli
enteroaggregative E. coli
adherence patterns
childhood diarrhea
Escherichia coli
E. coli que adere difusamente
E. coli enteroagregativa
padrões de adesão
diarréia infantil
title_short Diffuse and enteroaggregative patterns of adherence of Escherichia coli isolated from stools of children in northeastern Brazil
title_full Diffuse and enteroaggregative patterns of adherence of Escherichia coli isolated from stools of children in northeastern Brazil
title_fullStr Diffuse and enteroaggregative patterns of adherence of Escherichia coli isolated from stools of children in northeastern Brazil
title_full_unstemmed Diffuse and enteroaggregative patterns of adherence of Escherichia coli isolated from stools of children in northeastern Brazil
title_sort Diffuse and enteroaggregative patterns of adherence of Escherichia coli isolated from stools of children in northeastern Brazil
author Scaletsky, Isabel Cristina Affonso [UNIFESP]
author_facet Scaletsky, Isabel Cristina Affonso [UNIFESP]
Fabbricotti, Sandra Hilde [UNIFESP]
Carvalho, Rozane de Lima Bigelli [UNIFESP]
Nunes, Claudia Regina
Maranhão, Helcio de Sousa
Morais, Mauro Batista de [UNIFESP]
Riley, Lee
Fagundes-Neto, Ulysses [UNIFESP]
author_role author
author2 Fabbricotti, Sandra Hilde [UNIFESP]
Carvalho, Rozane de Lima Bigelli [UNIFESP]
Nunes, Claudia Regina
Maranhão, Helcio de Sousa
Morais, Mauro Batista de [UNIFESP]
Riley, Lee
Fagundes-Neto, Ulysses [UNIFESP]
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
Hospital Universitário Materno-Infantil
Universidade Federal do Rio Grande do Norte Departamento de Pediatria
School of Public Health
dc.contributor.author.fl_str_mv Scaletsky, Isabel Cristina Affonso [UNIFESP]
Fabbricotti, Sandra Hilde [UNIFESP]
Carvalho, Rozane de Lima Bigelli [UNIFESP]
Nunes, Claudia Regina
Maranhão, Helcio de Sousa
Morais, Mauro Batista de [UNIFESP]
Riley, Lee
Fagundes-Neto, Ulysses [UNIFESP]
dc.subject.por.fl_str_mv Escherichia coli
diffusely adherent E. coli
enteroaggregative E. coli
adherence patterns
childhood diarrhea
Escherichia coli
E. coli que adere difusamente
E. coli enteroagregativa
padrões de adesão
diarréia infantil
topic Escherichia coli
diffusely adherent E. coli
enteroaggregative E. coli
adherence patterns
childhood diarrhea
Escherichia coli
E. coli que adere difusamente
E. coli enteroagregativa
padrões de adesão
diarréia infantil
description Childhood diarrheal diseases remain highly endemic in northeastern Brazil. The attributable fraction of all diarrheal diseases among children less than 2 years of age due to Escherichia coli was examined in a 2-year prospective study in two large urban centers of Brazil. Between May 1997 and June 1999, fecal E. coli isolates from 237 children with diarrhea (217 acute and 20 persistent cases) and 231 children without diarrhea (controls) attending two hospitals in Northeast Brazil were tested for their pattern of adherence to HEp-2 cells and for colony hybridization with DNA probes specific for the six pathotypes of diarrheagenic E. coli. Enteroinvasive E. coli, enterotoxigenic E. coli and enterohemorrhagic E. coli were not isolated from any children. Diffusely adherent E. coli (DAEC) and enteroaggregative E. coli (EAEC) were the most frequent isolates with similar frequencies from children with or without diarrhea. Atypical EPEC (EAF-negative) strains were isolated with similiar frequency from both cases (5.5%) and controls (5.6%). Enteropathogenic E. coli (typical EPEC) strains, characterized by localized adherence pattern of adherence, hybridization with the EAF probe, and belonging to the classical O serogroups, were significantly associated with diarrhea (P = 0.03). These E. coli strains associated with diarrhea accounted for 9% of all children with diarrhea. Collectively, in Northeast Brazil, E. coli strains comprise a small proportion of severe diarrhea prevalence in children.
publishDate 2001
dc.date.none.fl_str_mv 2001-12-01
2015-06-14T13:29:33Z
2015-06-14T13:29:33Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1517-83822001000400012
Brazilian Journal of Microbiology. Sociedade Brasileira de Microbiologia, v. 32, n. 4, p. 313-319, 2001.
10.1590/S1517-83822001000400012
S1517-83822001000400012.pdf
1517-8382
S1517-83822001000400012
http://repositorio.unifesp.br/handle/11600/1298
WOS:000176404800012
url http://dx.doi.org/10.1590/S1517-83822001000400012
http://repositorio.unifesp.br/handle/11600/1298
identifier_str_mv Brazilian Journal of Microbiology. Sociedade Brasileira de Microbiologia, v. 32, n. 4, p. 313-319, 2001.
10.1590/S1517-83822001000400012
S1517-83822001000400012.pdf
1517-8382
S1517-83822001000400012
WOS:000176404800012
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Brazilian Journal of Microbiology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 313-319
application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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