Expression of HLA-DR in pheripheral nerve of amyotrophic lateral sclerosis

• Autor(a) principal: Oliveira, Acary Souza Bulle [UNIFESP]
• Data de Publicação: 1994
• Outros Autores: Isozaki, E., Younger, D., Gabbai, Alberto Alain [UNIFESP], Hays, A.p.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNIFESP
• dARK ID: ark:/48912/001300000mbvr
• DOI: 10.1590/S0004-282X1994000400007
• Texto Completo: http://dx.doi.org/10.1590/S0004-282X1994000400007; http://repositorio.unifesp.br/handle/11600/357
• topic: amyotrophic lateral sclerosis; peripheral nerve; histocompatibity antigen; esclerose lateral amiotrófica; nervo periférico; antígeno de histocompatibilidade

To investigate the possibility of local antigen presentation within the peripheral nerve in amyotrophic lateral sclerosis (ALS), cryostat sections of 83 peripheral nerve biopsies were stained for the demonstration of HLA-DR using a monoclonal antibody. Forty samples showed increased expression of HLA-DR in endoneurium. The phenotypic characteristics of the HLA-DR positive cells are chiefly Schwann cells, using S-100 protein as a marker. We did not detect any co-expression between HLA-DR and NF (axons) and HLA-DR and myelin marker. We also detected co-expression between HLA-DR and NGFr in a majority of HLA-DR positive cells. Inflammatory cells were infrequent, being detected only in 11 cases, predominantly around epineurial blood vessels. Motor and sensory nerve biopsies performed simultaneously showed higher expression of HLA-DR in motor nerves in 2 out of 4 patients. The significance of these findings is not clear. The presence of endoneurial cells expressing HLA-DR suggests that an autoimmune mechanism may be involved in ALS having Schwann as the main target.