Comparison among the BED capture enzyme immunoassay test and AxSYM avidity index assay for determining recent HIV infection and incidence in two Voluntary Counselling and Testing Centres in Northeast Brazil
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/8492 http://dx.doi.org/10.1016/j.bjid.2014.03.001 |
Resumo: | The aims of this study were to compare the automated AxSYM avidity assay index with the BED capture enzyme immunoassay test and to calculate the HIV-1 incidence using the BED capture enzyme immunoassay and AxSYM avidity assay index algorithms within a population seeking the Voluntary Counselling and Testing Centres in two municipalities in the Metropolitan Region of Recife, Northeast of Brazil. An analysis was conducted in 365 samples that tested positive for HIV infection from frozen serum collected during the period 2006-2009. There was a similar proportion of males and females; most patients were heterosexual (86%) with a median age of 29 years. Of the 365 samples, 102 (28%) and 66 (18.1%) were identified as recent infections by BED capture enzyme immunoassay and AxSYM avidity assay index, respectively. The HIV-1 total incidence in the BED capture enzyme immunoassay and AxSYM avidity assay index algorithms were: 0.79 (95% CI: 0.60-0.98) and 0.34 (95% CI: -0.04 to 0.72), respectively. Incidence was higher among men. There was good agreement between the tests, with a kappa of 0.654 and a specificity of 95.8%. AxSYM avidity assay index may be helpful in improving the quality of the estimates of recent HIV infection and incidence, particularly when used in a combined algorithm with BED capture enzyme immunoassay. |
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Salustiano, Daniela MedeirosLima, Kledoaldo Oliveira deCavalcanti, Ana Maria SalustianoDiaz, Ricardo Sobhie [UNIFESP]Lacerda, Heloisa RamosThe Herbert de Souza Counselling and Testing CentreUniversidade Federal de PernambucoThe Paulista Counselling and Testing CentreLaboratório Central de Pernambuco Department of VirologyUniversidade Federal de São Paulo (UNIFESP)2015-06-14T13:47:12Z2015-06-14T13:47:12Z2014-07-01Brazilian Journal of Infectious Diseases. Brazilian Society of Infectious Diseases, v. 18, n. 4, p. 449-453, 2014.1413-8670http://repositorio.unifesp.br/handle/11600/8492http://dx.doi.org/10.1016/j.bjid.2014.03.001S1413-86702014000400449.pdfS1413-8670201400040044910.1016/j.bjid.2014.03.001WOS:000341171200015The aims of this study were to compare the automated AxSYM avidity assay index with the BED capture enzyme immunoassay test and to calculate the HIV-1 incidence using the BED capture enzyme immunoassay and AxSYM avidity assay index algorithms within a population seeking the Voluntary Counselling and Testing Centres in two municipalities in the Metropolitan Region of Recife, Northeast of Brazil. An analysis was conducted in 365 samples that tested positive for HIV infection from frozen serum collected during the period 2006-2009. There was a similar proportion of males and females; most patients were heterosexual (86%) with a median age of 29 years. Of the 365 samples, 102 (28%) and 66 (18.1%) were identified as recent infections by BED capture enzyme immunoassay and AxSYM avidity assay index, respectively. The HIV-1 total incidence in the BED capture enzyme immunoassay and AxSYM avidity assay index algorithms were: 0.79 (95% CI: 0.60-0.98) and 0.34 (95% CI: -0.04 to 0.72), respectively. Incidence was higher among men. There was good agreement between the tests, with a kappa of 0.654 and a specificity of 95.8%. AxSYM avidity assay index may be helpful in improving the quality of the estimates of recent HIV infection and incidence, particularly when used in a combined algorithm with BED capture enzyme immunoassay.Programa Nacional de Cooperação AcadêmicaAção Novas Fronteiras (Procad - NF)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)The Herbert de Souza Counselling and Testing CentreUniversidade Federal de PernambucoThe Paulista Counselling and Testing CentreLaboratório Central de Pernambuco Department of VirologyUniversidade Federal de São Paulo (UNIFESP)UNIFESP, EPMSciELO449-453engBrazilian Society of Infectious DiseasesBrazilian Journal of Infectious DiseasesHIVRecent infectionAvidity index assayBED-CEIAComparison among the BED capture enzyme immunoassay test and AxSYM avidity index assay for determining recent HIV infection and incidence in two Voluntary Counselling and Testing Centres in Northeast Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALS1413-86702014000400449.pdfapplication/pdf479059${dspace.ui.url}/bitstream/11600/8492/1/S1413-86702014000400449.pdf7c4040627ad407f5f33857c8fb7fb3ccMD51open accessTEXTS1413-86702014000400449.pdf.txtS1413-86702014000400449.pdf.txtExtracted texttext/plain25241${dspace.ui.url}/bitstream/11600/8492/2/S1413-86702014000400449.pdf.txtb47c5d876a02c9c851feab1c99e29977MD52open access11600/84922022-07-08 10:54:12.075open accessoai:repositorio.unifesp.br:11600/8492Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652022-07-08T13:54:12Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Comparison among the BED capture enzyme immunoassay test and AxSYM avidity index assay for determining recent HIV infection and incidence in two Voluntary Counselling and Testing Centres in Northeast Brazil |
title |
Comparison among the BED capture enzyme immunoassay test and AxSYM avidity index assay for determining recent HIV infection and incidence in two Voluntary Counselling and Testing Centres in Northeast Brazil |
spellingShingle |
Comparison among the BED capture enzyme immunoassay test and AxSYM avidity index assay for determining recent HIV infection and incidence in two Voluntary Counselling and Testing Centres in Northeast Brazil Salustiano, Daniela Medeiros HIV Recent infection Avidity index assay BED-CEIA |
title_short |
Comparison among the BED capture enzyme immunoassay test and AxSYM avidity index assay for determining recent HIV infection and incidence in two Voluntary Counselling and Testing Centres in Northeast Brazil |
title_full |
Comparison among the BED capture enzyme immunoassay test and AxSYM avidity index assay for determining recent HIV infection and incidence in two Voluntary Counselling and Testing Centres in Northeast Brazil |
title_fullStr |
Comparison among the BED capture enzyme immunoassay test and AxSYM avidity index assay for determining recent HIV infection and incidence in two Voluntary Counselling and Testing Centres in Northeast Brazil |
title_full_unstemmed |
Comparison among the BED capture enzyme immunoassay test and AxSYM avidity index assay for determining recent HIV infection and incidence in two Voluntary Counselling and Testing Centres in Northeast Brazil |
title_sort |
Comparison among the BED capture enzyme immunoassay test and AxSYM avidity index assay for determining recent HIV infection and incidence in two Voluntary Counselling and Testing Centres in Northeast Brazil |
author |
Salustiano, Daniela Medeiros |
author_facet |
Salustiano, Daniela Medeiros Lima, Kledoaldo Oliveira de Cavalcanti, Ana Maria Salustiano Diaz, Ricardo Sobhie [UNIFESP] Lacerda, Heloisa Ramos |
author_role |
author |
author2 |
Lima, Kledoaldo Oliveira de Cavalcanti, Ana Maria Salustiano Diaz, Ricardo Sobhie [UNIFESP] Lacerda, Heloisa Ramos |
author2_role |
author author author author |
dc.contributor.institution.none.fl_str_mv |
The Herbert de Souza Counselling and Testing Centre Universidade Federal de Pernambuco The Paulista Counselling and Testing Centre Laboratório Central de Pernambuco Department of Virology Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Salustiano, Daniela Medeiros Lima, Kledoaldo Oliveira de Cavalcanti, Ana Maria Salustiano Diaz, Ricardo Sobhie [UNIFESP] Lacerda, Heloisa Ramos |
dc.subject.eng.fl_str_mv |
HIV Recent infection Avidity index assay BED-CEIA |
topic |
HIV Recent infection Avidity index assay BED-CEIA |
description |
The aims of this study were to compare the automated AxSYM avidity assay index with the BED capture enzyme immunoassay test and to calculate the HIV-1 incidence using the BED capture enzyme immunoassay and AxSYM avidity assay index algorithms within a population seeking the Voluntary Counselling and Testing Centres in two municipalities in the Metropolitan Region of Recife, Northeast of Brazil. An analysis was conducted in 365 samples that tested positive for HIV infection from frozen serum collected during the period 2006-2009. There was a similar proportion of males and females; most patients were heterosexual (86%) with a median age of 29 years. Of the 365 samples, 102 (28%) and 66 (18.1%) were identified as recent infections by BED capture enzyme immunoassay and AxSYM avidity assay index, respectively. The HIV-1 total incidence in the BED capture enzyme immunoassay and AxSYM avidity assay index algorithms were: 0.79 (95% CI: 0.60-0.98) and 0.34 (95% CI: -0.04 to 0.72), respectively. Incidence was higher among men. There was good agreement between the tests, with a kappa of 0.654 and a specificity of 95.8%. AxSYM avidity assay index may be helpful in improving the quality of the estimates of recent HIV infection and incidence, particularly when used in a combined algorithm with BED capture enzyme immunoassay. |
publishDate |
2014 |
dc.date.issued.fl_str_mv |
2014-07-01 |
dc.date.accessioned.fl_str_mv |
2015-06-14T13:47:12Z |
dc.date.available.fl_str_mv |
2015-06-14T13:47:12Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Brazilian Journal of Infectious Diseases. Brazilian Society of Infectious Diseases, v. 18, n. 4, p. 449-453, 2014. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/8492 http://dx.doi.org/10.1016/j.bjid.2014.03.001 |
dc.identifier.issn.none.fl_str_mv |
1413-8670 |
dc.identifier.file.none.fl_str_mv |
S1413-86702014000400449.pdf |
dc.identifier.scielo.none.fl_str_mv |
S1413-86702014000400449 |
dc.identifier.doi.none.fl_str_mv |
10.1016/j.bjid.2014.03.001 |
dc.identifier.wos.none.fl_str_mv |
WOS:000341171200015 |
identifier_str_mv |
Brazilian Journal of Infectious Diseases. Brazilian Society of Infectious Diseases, v. 18, n. 4, p. 449-453, 2014. 1413-8670 S1413-86702014000400449.pdf S1413-86702014000400449 10.1016/j.bjid.2014.03.001 WOS:000341171200015 |
url |
http://repositorio.unifesp.br/handle/11600/8492 http://dx.doi.org/10.1016/j.bjid.2014.03.001 |
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eng |
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dc.relation.ispartof.none.fl_str_mv |
Brazilian Journal of Infectious Diseases |
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openAccess |
dc.format.none.fl_str_mv |
449-453 |
dc.publisher.none.fl_str_mv |
Brazilian Society of Infectious Diseases |
publisher.none.fl_str_mv |
Brazilian Society of Infectious Diseases |
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