Hpv genotyping and p16 expression in xingu indigenous park, brazil

• Autor(a) principal: Freitas, V. G. [UNIFESP]
• Data de Publicação: 2016
• Outros Autores: Focchi, G. R. [UNIFESP], Pereira, E. R. [UNIFESP], Levi, J. E., Speck, N. M. G. [UNIFESP], Ribalta, J. C. [UNIFESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNIFESP
• Texto Completo: http://dx.doi.org/10.4238/gmr.15036840; http://repositorio.unifesp.br/handle/11600/49389
• topic: P16 Protein; P16 Expression; Human Papillomavirus; Indigenous; Xingu; GenotypeCervical Intraepithelial Neoplasia; Human-Papillomavirus Genotypes; Cancer; Women; Infection; Lesions; Diagnosis; Risk; Prevalence; Prevention

The association between high-risk human papillomavirus (HPV) genotypes and p16 expression in indigenous women from the Xingu Indigenous Park, Brazil, was unknown. This study evaluated p16 expression in women with a histological diagnosis of cervical intraepithelial neoplasia (CIN) 3 or higher and correlated this expression with HPV genotypes to determine possible discrepancies in the expression of this marker. We evaluated 37 previously collected samples with different HPV genotypes and high-grade lesions diagnosed based on cytology, histology, and colposcopy. Immunohistochemical analysis was performed using paraffin-embedded tissue sections and the CINtec (R) Histology Kit. p16 protein expression was investigated by immunostaining with an anti-p16 antibody. HPV genotyping was performed by reverse hybridization. The age of the study population ranged from 22-75 years (43.81 +/- 15.89 years) and parity ranged from 1-11 (5.92 +/- 2.58). Thirteen different HPV genotypes were found using the INNO-LiPA kit. Single and multiple infections by HPV were found with prevalence of single infections (P = 0.029). Comparison between HPV genotype and simple or multiple infections was highly significant