Specific components found in circulating immune complexes (CIC) in paracoccidioidomycosis

Detalhes bibliográficos
Autor(a) principal: Unterkircher, C. S.
Data de Publicação: 1996
Outros Autores: Yazaki, S. C., Shimizu, M. T., Jorge, AOC, Camargo, Z. P.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1080/02681219680000461
http://repositorio.unifesp.br/handle/11600/44494
Resumo: Circulating immune complexes (CIC) from 15 paracoccidioidomycosis (PCM) patient sera and from 20 healthy control sera were analysed. After CIC precipitation, solubilization and acid treatment, only a little reactivity to P. brasiliensis antigens was found in the free antibodies from PCM-CIC. This result has suggested that there were antibodies with a high affinity bound to fungus components. Dissociated CIC were fractionated in a column of Sephacryl S300 and the fractions that probably contained antigens were pooled and applied to an affinity column, prepared with mouse anti-gp43 monoclonal antibody. Using ECL-Western blotting assay two polypeptide with apparent mass of 43 and 62 kDa were found.
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spelling Specific components found in circulating immune complexes (CIC) in paracoccidioidomycosisCirculating immune complexes (CIC) from 15 paracoccidioidomycosis (PCM) patient sera and from 20 healthy control sera were analysed. After CIC precipitation, solubilization and acid treatment, only a little reactivity to P. brasiliensis antigens was found in the free antibodies from PCM-CIC. This result has suggested that there were antibodies with a high affinity bound to fungus components. Dissociated CIC were fractionated in a column of Sephacryl S300 and the fractions that probably contained antigens were pooled and applied to an affinity column, prepared with mouse anti-gp43 monoclonal antibody. Using ECL-Western blotting assay two polypeptide with apparent mass of 43 and 62 kDa were found.UNIV FED SAO PAULO,ESCOLA PAULISTA MED,DISCIPLINA BIOL CELULAR,BR-04023062 SAO PAULO,BRAZILUNESP,SCH DENT,DEPT PATHOL,SAO PAULO,BRAZILUNIV FED SAO PAULO,ESCOLA PAULISTA MED,DISCIPLINA BIOL CELULAR,BR-04023062 SAO PAULO,BRAZILWeb of ScienceBlackwell Science LtdUniversidade Federal de São Paulo (UNIFESP)UNESPUnterkircher, C. S.Yazaki, S. C.Shimizu, M. T.Jorge, AOCCamargo, Z. P.2018-06-15T18:07:24Z2018-06-15T18:07:24Z1996-07-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion273-277http://dx.doi.org/10.1080/02681219680000461Journal Of Medical And Veterinary Mycology. Oxford: Blackwell Science Ltd, v. 34, n. 4, p. 273-277, 1996.10.1080/026812196800004610268-1218http://repositorio.unifesp.br/handle/11600/44494WOS:A1996VC30200007engJournal Of Medical And Veterinary Mycologyinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-05-02T11:58:59Zoai:repositorio.unifesp.br/:11600/44494Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-05-02T11:58:59Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Specific components found in circulating immune complexes (CIC) in paracoccidioidomycosis
title Specific components found in circulating immune complexes (CIC) in paracoccidioidomycosis
spellingShingle Specific components found in circulating immune complexes (CIC) in paracoccidioidomycosis
Unterkircher, C. S.
title_short Specific components found in circulating immune complexes (CIC) in paracoccidioidomycosis
title_full Specific components found in circulating immune complexes (CIC) in paracoccidioidomycosis
title_fullStr Specific components found in circulating immune complexes (CIC) in paracoccidioidomycosis
title_full_unstemmed Specific components found in circulating immune complexes (CIC) in paracoccidioidomycosis
title_sort Specific components found in circulating immune complexes (CIC) in paracoccidioidomycosis
author Unterkircher, C. S.
author_facet Unterkircher, C. S.
Yazaki, S. C.
Shimizu, M. T.
Jorge, AOC
Camargo, Z. P.
author_role author
author2 Yazaki, S. C.
Shimizu, M. T.
Jorge, AOC
Camargo, Z. P.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
UNESP
dc.contributor.author.fl_str_mv Unterkircher, C. S.
Yazaki, S. C.
Shimizu, M. T.
Jorge, AOC
Camargo, Z. P.
description Circulating immune complexes (CIC) from 15 paracoccidioidomycosis (PCM) patient sera and from 20 healthy control sera were analysed. After CIC precipitation, solubilization and acid treatment, only a little reactivity to P. brasiliensis antigens was found in the free antibodies from PCM-CIC. This result has suggested that there were antibodies with a high affinity bound to fungus components. Dissociated CIC were fractionated in a column of Sephacryl S300 and the fractions that probably contained antigens were pooled and applied to an affinity column, prepared with mouse anti-gp43 monoclonal antibody. Using ECL-Western blotting assay two polypeptide with apparent mass of 43 and 62 kDa were found.
publishDate 1996
dc.date.none.fl_str_mv 1996-07-01
2018-06-15T18:07:24Z
2018-06-15T18:07:24Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1080/02681219680000461
Journal Of Medical And Veterinary Mycology. Oxford: Blackwell Science Ltd, v. 34, n. 4, p. 273-277, 1996.
10.1080/02681219680000461
0268-1218
http://repositorio.unifesp.br/handle/11600/44494
WOS:A1996VC30200007
url http://dx.doi.org/10.1080/02681219680000461
http://repositorio.unifesp.br/handle/11600/44494
identifier_str_mv Journal Of Medical And Veterinary Mycology. Oxford: Blackwell Science Ltd, v. 34, n. 4, p. 273-277, 1996.
10.1080/02681219680000461
0268-1218
WOS:A1996VC30200007
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal Of Medical And Veterinary Mycology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 273-277
dc.publisher.none.fl_str_mv Blackwell Science Ltd
publisher.none.fl_str_mv Blackwell Science Ltd
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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