Clonal relationships among Escherichia coli serogroup O6 isolates based on RAPD

Detalhes bibliográficos
Autor(a) principal: Pacheco, Ana Beatriz Furlanetto
Data de Publicação: 1997
Outros Autores: Guth, Beatriz Ernestina Cabilio [UNIFESP], Soares, Kellen C C, Almeida, Darcy Fontoura de, Ferreira, Luís Carlos de Souza
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1016/S0378-1097(97)00045-1
http://repositorio.unifesp.br/handle/11600/25704
Resumo: The genetic diversity in a group of Escherichia coli strains belonging to serogroup O6 but expressing different H antigens was investigated by random amplification of polymorphic DNA (RAPD). Isolates of serotypes H16, H1, H31, and non-motile (NM) strains were typed using a set of 3 primers with different G+C contents. the amplified band arrays allowed the identification of 3 main clonal clusters corresponding to each O:H serotype analyzed. Based on their RAPD profiles NEVI strains could be assigned to either H1 or H31 serotypes. the results indicate that the flagellar antigen and the RAPD fingerprint represent reliable clonal markers in this E. coli group.
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spelling Clonal relationships among Escherichia coli serogroup O6 isolates based on RAPDEscherichia coliRAPD typingclonal groupingserotypeThe genetic diversity in a group of Escherichia coli strains belonging to serogroup O6 but expressing different H antigens was investigated by random amplification of polymorphic DNA (RAPD). Isolates of serotypes H16, H1, H31, and non-motile (NM) strains were typed using a set of 3 primers with different G+C contents. the amplified band arrays allowed the identification of 3 main clonal clusters corresponding to each O:H serotype analyzed. Based on their RAPD profiles NEVI strains could be assigned to either H1 or H31 serotypes. the results indicate that the flagellar antigen and the RAPD fingerprint represent reliable clonal markers in this E. coli group.Universidade Federal de São Paulo,DEPT MICROBIOL IMMUNOL & PARASITOL,ESCOLA PAULISTA MED,BR-04023062 São Paulo,BRAZILUniversidade Federal de São Paulo,DEPT MICROBIOL IMMUNOL & PARASITOL,ESCOLA PAULISTA MED,BR-04023062 São Paulo,BRAZILWeb of ScienceElsevier B.V.Universidade Federal de São Paulo (UNIFESP)Pacheco, Ana Beatriz FurlanettoGuth, Beatriz Ernestina Cabilio [UNIFESP]Soares, Kellen C CAlmeida, Darcy Fontoura deFerreira, Luís Carlos de Souza2016-01-24T12:30:19Z2016-01-24T12:30:19Z1997-03-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion255-260http://dx.doi.org/10.1016/S0378-1097(97)00045-1Fems Microbiology Letters. Amsterdam: Elsevier B.V., v. 148, n. 2, p. 255-260, 1997.10.1016/S0378-1097(97)00045-10378-1097http://repositorio.unifesp.br/handle/11600/25704WOS:A1997WP01700022engFems Microbiology Lettersinfo:eu-repo/semantics/openAccesshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policyreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2016-01-24T10:30:19Zoai:repositorio.unifesp.br/:11600/25704Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652016-01-24T10:30:19Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Clonal relationships among Escherichia coli serogroup O6 isolates based on RAPD
title Clonal relationships among Escherichia coli serogroup O6 isolates based on RAPD
spellingShingle Clonal relationships among Escherichia coli serogroup O6 isolates based on RAPD
Pacheco, Ana Beatriz Furlanetto
Escherichia coli
RAPD typing
clonal grouping
serotype
title_short Clonal relationships among Escherichia coli serogroup O6 isolates based on RAPD
title_full Clonal relationships among Escherichia coli serogroup O6 isolates based on RAPD
title_fullStr Clonal relationships among Escherichia coli serogroup O6 isolates based on RAPD
title_full_unstemmed Clonal relationships among Escherichia coli serogroup O6 isolates based on RAPD
title_sort Clonal relationships among Escherichia coli serogroup O6 isolates based on RAPD
author Pacheco, Ana Beatriz Furlanetto
author_facet Pacheco, Ana Beatriz Furlanetto
Guth, Beatriz Ernestina Cabilio [UNIFESP]
Soares, Kellen C C
Almeida, Darcy Fontoura de
Ferreira, Luís Carlos de Souza
author_role author
author2 Guth, Beatriz Ernestina Cabilio [UNIFESP]
Soares, Kellen C C
Almeida, Darcy Fontoura de
Ferreira, Luís Carlos de Souza
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Pacheco, Ana Beatriz Furlanetto
Guth, Beatriz Ernestina Cabilio [UNIFESP]
Soares, Kellen C C
Almeida, Darcy Fontoura de
Ferreira, Luís Carlos de Souza
dc.subject.por.fl_str_mv Escherichia coli
RAPD typing
clonal grouping
serotype
topic Escherichia coli
RAPD typing
clonal grouping
serotype
description The genetic diversity in a group of Escherichia coli strains belonging to serogroup O6 but expressing different H antigens was investigated by random amplification of polymorphic DNA (RAPD). Isolates of serotypes H16, H1, H31, and non-motile (NM) strains were typed using a set of 3 primers with different G+C contents. the amplified band arrays allowed the identification of 3 main clonal clusters corresponding to each O:H serotype analyzed. Based on their RAPD profiles NEVI strains could be assigned to either H1 or H31 serotypes. the results indicate that the flagellar antigen and the RAPD fingerprint represent reliable clonal markers in this E. coli group.
publishDate 1997
dc.date.none.fl_str_mv 1997-03-15
2016-01-24T12:30:19Z
2016-01-24T12:30:19Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/S0378-1097(97)00045-1
Fems Microbiology Letters. Amsterdam: Elsevier B.V., v. 148, n. 2, p. 255-260, 1997.
10.1016/S0378-1097(97)00045-1
0378-1097
http://repositorio.unifesp.br/handle/11600/25704
WOS:A1997WP01700022
url http://dx.doi.org/10.1016/S0378-1097(97)00045-1
http://repositorio.unifesp.br/handle/11600/25704
identifier_str_mv Fems Microbiology Letters. Amsterdam: Elsevier B.V., v. 148, n. 2, p. 255-260, 1997.
10.1016/S0378-1097(97)00045-1
0378-1097
WOS:A1997WP01700022
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Fems Microbiology Letters
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
eu_rights_str_mv openAccess
rights_invalid_str_mv http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.format.none.fl_str_mv 255-260
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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