Structural and functional changes in the alveolar bone osteoclasts of estrogen-treated rats
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/34355 http://dx.doi.org/10.1111/j.1469-7580.2011.01449.x |
Resumo: | This study investigated structural and functional features of apoptotic alveolar bone osteoclasts in estrogen-treated rats. for this purpose, 15 female rats 22 days old were divided into three groups: Estrogen (EG), Sham (SG) and Control (CG). the rats of EG received daily intramuscular injection of estrogen for 7 days. the SG received only the oil vehicle. Maxillary fragments containing alveolar bone were removed and processed for light and transmission electron microscopy. Area (OcA) and number of nuclei (OcN) and bone resorption surface per TRAP-positive osteoclasts (BS/OC) were obtained. Vimentin, caspase-3 and MMP-9 immunoreactions, TUNEL/TRAP and MMP-9/TUNEL combined reactions were performed. in EG, the OcA, OcN and BS/Oc were reduced. Moreover, osteoclasts showed cytoplasm immunolabelled by caspase-3 and a different pattern of vimentin expression in comparison with CG and SG. MMP-9 expression was not affected by estrogen and the TUNEL-positive osteoclasts were MMP-9-immunolabelled. in EG, ultrastructural images showed that apoptotic osteoclasts did not exhibit ruffled borders or clear zones and were shedding mononucleated portions. TRAP-positive structures containing irregular and dense chromatin were partially surrounded by fibroblast-like cells. in conclusion, the reduction in the BS/Oc may be due to reduction in OcA and OcN; these effects seem to be related to vimentin disarrangement rather than to an interference of estrogen with osteoclast MMP-9 expression. Osteoclast apoptosis involves caspase-3 activity and vimentin degradation; these cells release portions containing one apoptotic nucleus and, subsequently, undergo fragmentation, giving rise to apoptotic bodies. |
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Faloni, Ana Paula de Souza [UNIFESP]Sasso-Cerri, Estela [UNIFESP]Godoy Rocha, Fernanda ReginaKatchburian, Eduardo [UNIFESP]Cerri, Paulo Sergio [UNIFESP]UNESP Univ Estadual PaulistaUniversidade Federal de São Paulo (UNIFESP)2016-01-24T14:17:36Z2016-01-24T14:17:36Z2012-01-01Journal of Anatomy. Malden: Wiley-Blackwell, v. 220, n. 1, p. 77-85, 2012.0021-8782http://repositorio.unifesp.br/handle/11600/34355http://dx.doi.org/10.1111/j.1469-7580.2011.01449.x10.1111/j.1469-7580.2011.01449.xWOS:000297947100008This study investigated structural and functional features of apoptotic alveolar bone osteoclasts in estrogen-treated rats. for this purpose, 15 female rats 22 days old were divided into three groups: Estrogen (EG), Sham (SG) and Control (CG). the rats of EG received daily intramuscular injection of estrogen for 7 days. the SG received only the oil vehicle. Maxillary fragments containing alveolar bone were removed and processed for light and transmission electron microscopy. Area (OcA) and number of nuclei (OcN) and bone resorption surface per TRAP-positive osteoclasts (BS/OC) were obtained. Vimentin, caspase-3 and MMP-9 immunoreactions, TUNEL/TRAP and MMP-9/TUNEL combined reactions were performed. in EG, the OcA, OcN and BS/Oc were reduced. Moreover, osteoclasts showed cytoplasm immunolabelled by caspase-3 and a different pattern of vimentin expression in comparison with CG and SG. MMP-9 expression was not affected by estrogen and the TUNEL-positive osteoclasts were MMP-9-immunolabelled. in EG, ultrastructural images showed that apoptotic osteoclasts did not exhibit ruffled borders or clear zones and were shedding mononucleated portions. TRAP-positive structures containing irregular and dense chromatin were partially surrounded by fibroblast-like cells. in conclusion, the reduction in the BS/Oc may be due to reduction in OcA and OcN; these effects seem to be related to vimentin disarrangement rather than to an interference of estrogen with osteoclast MMP-9 expression. Osteoclast apoptosis involves caspase-3 activity and vimentin degradation; these cells release portions containing one apoptotic nucleus and, subsequently, undergo fragmentation, giving rise to apoptotic bodies.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação para o Desenvolvimento da UNESP (FUNDUNESP)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)UNESP Univ Estadual Paulista, Dept Morphol, Sch Dent, Lab Histol & Embryol, BR-14801903 São Paulo, BrazilFed Univ São Paulo UNIFESP, Dept Morphol & Genet, São Paulo, BrazilFed Univ São Paulo UNIFESP, Dept Morphol & Genet, São Paulo, BrazilFAPESP: 04/09898-0Web of Science77-85engWiley-BlackwellJournal of Anatomyhttp://olabout.wiley.com/WileyCDA/Section/id-406071.htmlinfo:eu-repo/semantics/openAccessapoptosisestradiolMMP-9osteoclastvimentinStructural and functional changes in the alveolar bone osteoclasts of estrogen-treated ratsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlereponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP11600/343552022-09-27 09:59:59.306metadata only accessoai:repositorio.unifesp.br:11600/34355Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652022-09-27T12:59:59Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Structural and functional changes in the alveolar bone osteoclasts of estrogen-treated rats |
title |
Structural and functional changes in the alveolar bone osteoclasts of estrogen-treated rats |
spellingShingle |
Structural and functional changes in the alveolar bone osteoclasts of estrogen-treated rats Faloni, Ana Paula de Souza [UNIFESP] apoptosis estradiol MMP-9 osteoclast vimentin |
title_short |
Structural and functional changes in the alveolar bone osteoclasts of estrogen-treated rats |
title_full |
Structural and functional changes in the alveolar bone osteoclasts of estrogen-treated rats |
title_fullStr |
Structural and functional changes in the alveolar bone osteoclasts of estrogen-treated rats |
title_full_unstemmed |
Structural and functional changes in the alveolar bone osteoclasts of estrogen-treated rats |
title_sort |
Structural and functional changes in the alveolar bone osteoclasts of estrogen-treated rats |
author |
Faloni, Ana Paula de Souza [UNIFESP] |
author_facet |
Faloni, Ana Paula de Souza [UNIFESP] Sasso-Cerri, Estela [UNIFESP] Godoy Rocha, Fernanda Regina Katchburian, Eduardo [UNIFESP] Cerri, Paulo Sergio [UNIFESP] |
author_role |
author |
author2 |
Sasso-Cerri, Estela [UNIFESP] Godoy Rocha, Fernanda Regina Katchburian, Eduardo [UNIFESP] Cerri, Paulo Sergio [UNIFESP] |
author2_role |
author author author author |
dc.contributor.institution.none.fl_str_mv |
UNESP Univ Estadual Paulista Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Faloni, Ana Paula de Souza [UNIFESP] Sasso-Cerri, Estela [UNIFESP] Godoy Rocha, Fernanda Regina Katchburian, Eduardo [UNIFESP] Cerri, Paulo Sergio [UNIFESP] |
dc.subject.eng.fl_str_mv |
apoptosis estradiol MMP-9 osteoclast vimentin |
topic |
apoptosis estradiol MMP-9 osteoclast vimentin |
description |
This study investigated structural and functional features of apoptotic alveolar bone osteoclasts in estrogen-treated rats. for this purpose, 15 female rats 22 days old were divided into three groups: Estrogen (EG), Sham (SG) and Control (CG). the rats of EG received daily intramuscular injection of estrogen for 7 days. the SG received only the oil vehicle. Maxillary fragments containing alveolar bone were removed and processed for light and transmission electron microscopy. Area (OcA) and number of nuclei (OcN) and bone resorption surface per TRAP-positive osteoclasts (BS/OC) were obtained. Vimentin, caspase-3 and MMP-9 immunoreactions, TUNEL/TRAP and MMP-9/TUNEL combined reactions were performed. in EG, the OcA, OcN and BS/Oc were reduced. Moreover, osteoclasts showed cytoplasm immunolabelled by caspase-3 and a different pattern of vimentin expression in comparison with CG and SG. MMP-9 expression was not affected by estrogen and the TUNEL-positive osteoclasts were MMP-9-immunolabelled. in EG, ultrastructural images showed that apoptotic osteoclasts did not exhibit ruffled borders or clear zones and were shedding mononucleated portions. TRAP-positive structures containing irregular and dense chromatin were partially surrounded by fibroblast-like cells. in conclusion, the reduction in the BS/Oc may be due to reduction in OcA and OcN; these effects seem to be related to vimentin disarrangement rather than to an interference of estrogen with osteoclast MMP-9 expression. Osteoclast apoptosis involves caspase-3 activity and vimentin degradation; these cells release portions containing one apoptotic nucleus and, subsequently, undergo fragmentation, giving rise to apoptotic bodies. |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012-01-01 |
dc.date.accessioned.fl_str_mv |
2016-01-24T14:17:36Z |
dc.date.available.fl_str_mv |
2016-01-24T14:17:36Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Journal of Anatomy. Malden: Wiley-Blackwell, v. 220, n. 1, p. 77-85, 2012. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/34355 http://dx.doi.org/10.1111/j.1469-7580.2011.01449.x |
dc.identifier.issn.none.fl_str_mv |
0021-8782 |
dc.identifier.doi.none.fl_str_mv |
10.1111/j.1469-7580.2011.01449.x |
dc.identifier.wos.none.fl_str_mv |
WOS:000297947100008 |
identifier_str_mv |
Journal of Anatomy. Malden: Wiley-Blackwell, v. 220, n. 1, p. 77-85, 2012. 0021-8782 10.1111/j.1469-7580.2011.01449.x WOS:000297947100008 |
url |
http://repositorio.unifesp.br/handle/11600/34355 http://dx.doi.org/10.1111/j.1469-7580.2011.01449.x |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Journal of Anatomy |
dc.rights.driver.fl_str_mv |
http://olabout.wiley.com/WileyCDA/Section/id-406071.html info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://olabout.wiley.com/WileyCDA/Section/id-406071.html |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
77-85 |
dc.publisher.none.fl_str_mv |
Wiley-Blackwell |
publisher.none.fl_str_mv |
Wiley-Blackwell |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
|
_version_ |
1802764165917442048 |