Prebiotic and Synbiotic Modifications of Beta Oxidation and Lipogenic Gene Expression after Experimental Hypercholesterolemia in Rat Liver
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | https://repositorio.unifesp.br/handle/11600/57139 https://dx.doi.org/10.3389/fmicb.2017.02010 |
Resumo: | Background and aims: Non-alcoholic fatty liver disease (NAFLD) is characterized by the presence of fat in hepatocytes because of decreased β-oxidation and increased lipogenesis. Prebiotics, probiotics, and synbiotic have modulatory effects on intestinal microbiota and may influence the gut-liver axis. Our aim was to evaluate the effects of prebiotic, probiotics, and synbiotic on liver histopathology and gene expression related to β-oxidation and lipogenesis after hypercholesterolemia. Methods: Wistar male adult rats (n = 40) were submitted to hypercholesterolemic conditions (HPC) (60 days). On Day 30 of HPC, rats were subdivided in 5 groups: negative control (NC): without HPC + Gv (distilled water); positive control (PC): with HPC + Gv (distilled water); prebiotic (PRE): HPC + Gv with prebiotic (Fiber FOS®); probiotic (PRO): HPC + Gv with probiotic strains Gv (Probiatop®); and synbiotic (SYN): HPC + Gv with synbiotic (Simbioflora®). All rats were sacrificed on Day 30 post-treatment. Blood was collected to verify total serum cholesterol, and liver tissue was sampled to verify histopathological changes and gene expression. Gene expression related to ß-oxidation (PPAR-α and CPT-1) and lipogenesis (SREBP-1c, FAS and ME) was evaluated in liver tissue using RT-qPCR. Results: PC had higher cholesterol levels when compared to NC. PRE and SYN rats had lower cholesterol levels than PC. PC rats showed more histopathological changes than NC rats; PRE and SYN rats showed fewer alterations than PC rats. PPAR-α was expressed at higher levels in SYN and PC rats compared with PRE and PRO rats. CPT-1 expression was similar in all groups. SREBP-1c was expressed at higher levels in PC rats compared with NC rats; levels were lower in SYN rats compared with PRO rats; levels were lower in PRE rats compared with PC and PRO rats. FAS was expressed at lower levels in PRE rats compared with SYN rats. ME expression was lower in PC rats compared with NC rats. Conclusion: Prebiotic and synbiotic supplementation improve hepatic alterations related to hypercholesterolemia. These changes appear to be mediated by altered expression of genes related to β-oxidation and lipogenesis. |
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Alves, Claudia Cristina [UNIFESP]Waitzberg, Dan LinetzkyAndrade, Laila Santos de [UNIFESP]Aguiar, Lais dos Santos [UNIFESP]Reis, Milene Barcelos [UNIFESP]Guanabara, Camila Chaves [UNIFESP]Aguiar, Odair [UNIFESP]Ribeiro, Daniel Araki [UNIFESP]Sala, Priscila2020-08-04T13:39:49Z2020-08-04T13:39:49Z2017Frontiers In Microbiology. Lausanne, v. 8, p. -, 2017.1664-302Xhttps://repositorio.unifesp.br/handle/11600/57139https://dx.doi.org/10.3389/fmicb.2017.02010WOS000413105800001.pdf10.3389/fmicb.2017.02010WOS:000413105800001Background and aims: Non-alcoholic fatty liver disease (NAFLD) is characterized by the presence of fat in hepatocytes because of decreased β-oxidation and increased lipogenesis. Prebiotics, probiotics, and synbiotic have modulatory effects on intestinal microbiota and may influence the gut-liver axis. Our aim was to evaluate the effects of prebiotic, probiotics, and synbiotic on liver histopathology and gene expression related to β-oxidation and lipogenesis after hypercholesterolemia. Methods: Wistar male adult rats (n = 40) were submitted to hypercholesterolemic conditions (HPC) (60 days). On Day 30 of HPC, rats were subdivided in 5 groups: negative control (NC): without HPC + Gv (distilled water); positive control (PC): with HPC + Gv (distilled water); prebiotic (PRE): HPC + Gv with prebiotic (Fiber FOS®); probiotic (PRO): HPC + Gv with probiotic strains Gv (Probiatop®); and synbiotic (SYN): HPC + Gv with synbiotic (Simbioflora®). All rats were sacrificed on Day 30 post-treatment. Blood was collected to verify total serum cholesterol, and liver tissue was sampled to verify histopathological changes and gene expression. Gene expression related to ß-oxidation (PPAR-α and CPT-1) and lipogenesis (SREBP-1c, FAS and ME) was evaluated in liver tissue using RT-qPCR. Results: PC had higher cholesterol levels when compared to NC. PRE and SYN rats had lower cholesterol levels than PC. PC rats showed more histopathological changes than NC rats; PRE and SYN rats showed fewer alterations than PC rats. PPAR-α was expressed at higher levels in SYN and PC rats compared with PRE and PRO rats. CPT-1 expression was similar in all groups. SREBP-1c was expressed at higher levels in PC rats compared with NC rats; levels were lower in SYN rats compared with PRO rats; levels were lower in PRE rats compared with PC and PRO rats. FAS was expressed at lower levels in PRE rats compared with SYN rats. ME expression was lower in PC rats compared with NC rats. Conclusion: Prebiotic and synbiotic supplementation improve hepatic alterations related to hypercholesterolemia. These changes appear to be mediated by altered expression of genes related to β-oxidation and lipogenesis.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Fed Sao Paulo, Dept Biosci, Santos, BrazilUniv Sao Paulo, Dept Gastroenterol, Sch Med, Sao Paulo, BrazilUniv Fed Sao Paulo, Nutr Grad, Santos, BrazilUniv Fed Sao Paulo, Dept Biosci, Santos, BrazilUniv Fed Sao Paulo, Nutr Grad, Santos, BrazilFAPESP: 2011/50289-1Web of Science-engFrontiers Media SaFrontiers In MicrobiologyCholesterol dietSteatosisRatsPrebioticProbioticSynbioticGene expressionPrebiotic and Synbiotic Modifications of Beta Oxidation and Lipogenic Gene Expression after Experimental Hypercholesterolemia in Rat Liverinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleLausanne8info:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000413105800001.pdfapplication/pdf1075533${dspace.ui.url}/bitstream/11600/57139/1/WOS000413105800001.pdf5add3722f8ae0fc8a8e143c579171316MD51open accessTEXTWOS000413105800001.pdf.txtWOS000413105800001.pdf.txtExtracted texttext/plain46589${dspace.ui.url}/bitstream/11600/57139/5/WOS000413105800001.pdf.txt2ad233c73da95e9931346daac4ae863bMD55open accessTHUMBNAILWOS000413105800001.pdf.jpgWOS000413105800001.pdf.jpgIM Thumbnailimage/jpeg7536${dspace.ui.url}/bitstream/11600/57139/7/WOS000413105800001.pdf.jpg5feeced93d3f4ed587cacbe0134cf2f8MD57open access11600/571392023-06-05 19:13:53.256open accessoai:repositorio.unifesp.br:11600/57139Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-06-05T22:13:53Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Prebiotic and Synbiotic Modifications of Beta Oxidation and Lipogenic Gene Expression after Experimental Hypercholesterolemia in Rat Liver |
title |
Prebiotic and Synbiotic Modifications of Beta Oxidation and Lipogenic Gene Expression after Experimental Hypercholesterolemia in Rat Liver |
spellingShingle |
Prebiotic and Synbiotic Modifications of Beta Oxidation and Lipogenic Gene Expression after Experimental Hypercholesterolemia in Rat Liver Alves, Claudia Cristina [UNIFESP] Cholesterol diet Steatosis Rats Prebiotic Probiotic Synbiotic Gene expression |
title_short |
Prebiotic and Synbiotic Modifications of Beta Oxidation and Lipogenic Gene Expression after Experimental Hypercholesterolemia in Rat Liver |
title_full |
Prebiotic and Synbiotic Modifications of Beta Oxidation and Lipogenic Gene Expression after Experimental Hypercholesterolemia in Rat Liver |
title_fullStr |
Prebiotic and Synbiotic Modifications of Beta Oxidation and Lipogenic Gene Expression after Experimental Hypercholesterolemia in Rat Liver |
title_full_unstemmed |
Prebiotic and Synbiotic Modifications of Beta Oxidation and Lipogenic Gene Expression after Experimental Hypercholesterolemia in Rat Liver |
title_sort |
Prebiotic and Synbiotic Modifications of Beta Oxidation and Lipogenic Gene Expression after Experimental Hypercholesterolemia in Rat Liver |
author |
Alves, Claudia Cristina [UNIFESP] |
author_facet |
Alves, Claudia Cristina [UNIFESP] Waitzberg, Dan Linetzky Andrade, Laila Santos de [UNIFESP] Aguiar, Lais dos Santos [UNIFESP] Reis, Milene Barcelos [UNIFESP] Guanabara, Camila Chaves [UNIFESP] Aguiar, Odair [UNIFESP] Ribeiro, Daniel Araki [UNIFESP] Sala, Priscila |
author_role |
author |
author2 |
Waitzberg, Dan Linetzky Andrade, Laila Santos de [UNIFESP] Aguiar, Lais dos Santos [UNIFESP] Reis, Milene Barcelos [UNIFESP] Guanabara, Camila Chaves [UNIFESP] Aguiar, Odair [UNIFESP] Ribeiro, Daniel Araki [UNIFESP] Sala, Priscila |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Alves, Claudia Cristina [UNIFESP] Waitzberg, Dan Linetzky Andrade, Laila Santos de [UNIFESP] Aguiar, Lais dos Santos [UNIFESP] Reis, Milene Barcelos [UNIFESP] Guanabara, Camila Chaves [UNIFESP] Aguiar, Odair [UNIFESP] Ribeiro, Daniel Araki [UNIFESP] Sala, Priscila |
dc.subject.eng.fl_str_mv |
Cholesterol diet Steatosis Rats Prebiotic Probiotic Synbiotic Gene expression |
topic |
Cholesterol diet Steatosis Rats Prebiotic Probiotic Synbiotic Gene expression |
description |
Background and aims: Non-alcoholic fatty liver disease (NAFLD) is characterized by the presence of fat in hepatocytes because of decreased β-oxidation and increased lipogenesis. Prebiotics, probiotics, and synbiotic have modulatory effects on intestinal microbiota and may influence the gut-liver axis. Our aim was to evaluate the effects of prebiotic, probiotics, and synbiotic on liver histopathology and gene expression related to β-oxidation and lipogenesis after hypercholesterolemia. Methods: Wistar male adult rats (n = 40) were submitted to hypercholesterolemic conditions (HPC) (60 days). On Day 30 of HPC, rats were subdivided in 5 groups: negative control (NC): without HPC + Gv (distilled water); positive control (PC): with HPC + Gv (distilled water); prebiotic (PRE): HPC + Gv with prebiotic (Fiber FOS®); probiotic (PRO): HPC + Gv with probiotic strains Gv (Probiatop®); and synbiotic (SYN): HPC + Gv with synbiotic (Simbioflora®). All rats were sacrificed on Day 30 post-treatment. Blood was collected to verify total serum cholesterol, and liver tissue was sampled to verify histopathological changes and gene expression. Gene expression related to ß-oxidation (PPAR-α and CPT-1) and lipogenesis (SREBP-1c, FAS and ME) was evaluated in liver tissue using RT-qPCR. Results: PC had higher cholesterol levels when compared to NC. PRE and SYN rats had lower cholesterol levels than PC. PC rats showed more histopathological changes than NC rats; PRE and SYN rats showed fewer alterations than PC rats. PPAR-α was expressed at higher levels in SYN and PC rats compared with PRE and PRO rats. CPT-1 expression was similar in all groups. SREBP-1c was expressed at higher levels in PC rats compared with NC rats; levels were lower in SYN rats compared with PRO rats; levels were lower in PRE rats compared with PC and PRO rats. FAS was expressed at lower levels in PRE rats compared with SYN rats. ME expression was lower in PC rats compared with NC rats. Conclusion: Prebiotic and synbiotic supplementation improve hepatic alterations related to hypercholesterolemia. These changes appear to be mediated by altered expression of genes related to β-oxidation and lipogenesis. |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017 |
dc.date.accessioned.fl_str_mv |
2020-08-04T13:39:49Z |
dc.date.available.fl_str_mv |
2020-08-04T13:39:49Z |
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info:eu-repo/semantics/publishedVersion |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
Frontiers In Microbiology. Lausanne, v. 8, p. -, 2017. |
dc.identifier.uri.fl_str_mv |
https://repositorio.unifesp.br/handle/11600/57139 https://dx.doi.org/10.3389/fmicb.2017.02010 |
dc.identifier.issn.none.fl_str_mv |
1664-302X |
dc.identifier.file.none.fl_str_mv |
WOS000413105800001.pdf |
dc.identifier.doi.none.fl_str_mv |
10.3389/fmicb.2017.02010 |
dc.identifier.wos.none.fl_str_mv |
WOS:000413105800001 |
identifier_str_mv |
Frontiers In Microbiology. Lausanne, v. 8, p. -, 2017. 1664-302X WOS000413105800001.pdf 10.3389/fmicb.2017.02010 WOS:000413105800001 |
url |
https://repositorio.unifesp.br/handle/11600/57139 https://dx.doi.org/10.3389/fmicb.2017.02010 |
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eng |
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Frontiers In Microbiology |
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Frontiers Media Sa |
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Frontiers Media Sa |
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