Vitiligo: analysis of grafting versus curettage alone, using melanocyte morphology and reverse transcriptase polymerase chain reaction for tyrosinase mRNA

Detalhes bibliográficos
Autor(a) principal: Machado Filho, Carlos D'Apparecida Santos [UNIFESP]
Data de Publicação: 2005
Outros Autores: Almeida, Fernando Augusto [UNIFESP], Proto, Rodrigo Sestito, Landman, Gilles [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1590/S1516-31802005000400006
http://repositorio.unifesp.br/handle/11600/2366
Resumo: CONTEXT AND OBJECTIVE: Recent studies have indicated that vitiligo areas contain inactive or dormant melanocytes. Melanin synthesis is related to tyrosinase presence and indicative of active metabolic state. The aim of this study was to compare repigmentation, epidermal melanocyte distribution and tyrosinase mRNA detection through reverse transcriptase polymerase chain reaction, in tissue samples of vitiligo, before and after curettage, with or without subsequent autologous skin graft using a new method. DESIGN AND SETTING: Prospective, in the Department of Dermatology, Faculdade de Medicina do ABC, Santo André. METHODS: Two vitiligo areas were curetted. One subsequently received grafted normal sacral autologous skin, whereas the other had no further treatment. The curetted areas were examined after 30 days, to evaluate the degree of repigmentation. The melanocyte percentages and tyrosinase mRNA presence in normal skin and vitiligo areas, before and after curettage and grafting, were compared. RESULTS: Complete repigmentation was seen in all grafted areas, whereas non-grafted curetted vitiligo presented partial repigmentation. The melanocyte percentage in grafted areas was greater than in non-treated vitiligo skin (p = 0.01) and skin with curettage alone (p = 0.015). Tyrosinase mRNA was negative in 93.75% of non-treated vitiligo areas. After treatment (curettage alone or curettage and grafting), all lesions became positive for tyrosinase mRNA. CONCLUSION: Metabolically inactive or dormant melanocytes are probably present within vitiligo areas, and may be activated by exogenous or endogenous stimuli.
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spelling Vitiligo: analysis of grafting versus curettage alone, using melanocyte morphology and reverse transcriptase polymerase chain reaction for tyrosinase mRNAVitiligo: análise da área enxertada comparada com curetagem isolada através de morfologia celular e transcriptase reversa da reação de polimerase em cadeia do RNA mensageiro da tirosinaseVitiligoTransplantsTyrosinasePolymerase chain reactionPathologyVitiligoTransplantesTirosinaseReação em cadeia de polimerasePatologiaCONTEXT AND OBJECTIVE: Recent studies have indicated that vitiligo areas contain inactive or dormant melanocytes. Melanin synthesis is related to tyrosinase presence and indicative of active metabolic state. The aim of this study was to compare repigmentation, epidermal melanocyte distribution and tyrosinase mRNA detection through reverse transcriptase polymerase chain reaction, in tissue samples of vitiligo, before and after curettage, with or without subsequent autologous skin graft using a new method. DESIGN AND SETTING: Prospective, in the Department of Dermatology, Faculdade de Medicina do ABC, Santo André. METHODS: Two vitiligo areas were curetted. One subsequently received grafted normal sacral autologous skin, whereas the other had no further treatment. The curetted areas were examined after 30 days, to evaluate the degree of repigmentation. The melanocyte percentages and tyrosinase mRNA presence in normal skin and vitiligo areas, before and after curettage and grafting, were compared. RESULTS: Complete repigmentation was seen in all grafted areas, whereas non-grafted curetted vitiligo presented partial repigmentation. The melanocyte percentage in grafted areas was greater than in non-treated vitiligo skin (p = 0.01) and skin with curettage alone (p = 0.015). Tyrosinase mRNA was negative in 93.75% of non-treated vitiligo areas. After treatment (curettage alone or curettage and grafting), all lesions became positive for tyrosinase mRNA. CONCLUSION: Metabolically inactive or dormant melanocytes are probably present within vitiligo areas, and may be activated by exogenous or endogenous stimuli.CONTEXTO E OBJETIVO: Estudos recentes têm indicado que áreas de vitiligo contêm melanócitos inativos ou dormentes. A síntese de melanina está relacionada com a presença de tirosinase é indicadora de estado metabólico ativo. O escopo deste artigo é comparar repigmentação, distribuição epidérmica de melanócitos e detecção do RNAm de tirosinase através da reação de polimerase em cadeia por transcriptase reversa, em amostras de tecido de vitiligo, antes e após curetagem, seguido ou não por uma nova técnica de enxerto autólogo de pele. TIPO DE ESTUDO E LOCAL: Prospectivo, na Disciplina de Dermatologia da Faculdade de Medicina do ABC, Santo André. MÉTODOS: Duas áreas de vitiligo foram curetadas. Uma foi subseqüentemente enxertada com pele autóloga sacral normal, enquanto a outra não recebeu nenhum enxerto. As áreas curetadas foram examinadas após 30 dias, para avaliar o grau de repigmentação. A porcentagem de melanócitos e a presença de RNAm de tirosinase de pele normal e de pele de vitiligo (antes e após curetagem apenas e antes e após curetagem seguida de enxertia) foram comparadas. RESULTADOS: Repigmentação completa foi observada em todas as áreas enxertadas, enquanto as áreas de vitiligo apenas curetadas apresentaram repigmentação parcial. A porcentagem de melanócitos nas áreas enxertadas excederam as áreas de vitiligo não tratados (p = 0.01) e as áreas tratadas com curetagem simples (p = 0,015). RNAm de tirosinase foi negativo em 93.75% das áreas não tratadas de vitiligo. Após tratamento (somente curetagem ou curetagem seguida de enxerto), todas as lesões tornaram-se positivas para RNAm de tirosinase. CONCLUSÃO: Melanócitos metabolicamente inativos ou dormentes estão provavelmente presentes dentro das áreas de vitiligo, e podem ser ativados por estímulos endógenos ou exógenos.Fundação do ABC Faculdade de Medicina Department of DermatologyUniversidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Department of DermatologyHospital do Câncer Treatment and Research CenterUNIFESP, EPM, Department of DermatologySciELOAssociação Paulista de Medicina - APMFundação do ABC Faculdade de Medicina Department of DermatologyUniversidade Federal de São Paulo (UNIFESP)Hospital do Câncer Treatment and Research CenterMachado Filho, Carlos D'Apparecida Santos [UNIFESP]Almeida, Fernando Augusto [UNIFESP]Proto, Rodrigo SestitoLandman, Gilles [UNIFESP]2015-06-14T13:31:24Z2015-06-14T13:31:24Z2005-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion187-191application/pdfhttp://dx.doi.org/10.1590/S1516-31802005000400006São Paulo Medical Journal. Associação Paulista de Medicina - APM, v. 123, n. 4, p. 187-191, 2005.10.1590/S1516-31802005000400006S1516-31802005000400006.pdf1516-3180S1516-31802005000400006http://repositorio.unifesp.br/handle/11600/2366engSão Paulo Medical Journalinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-07-30T00:55:48Zoai:repositorio.unifesp.br/:11600/2366Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-07-30T00:55:48Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Vitiligo: analysis of grafting versus curettage alone, using melanocyte morphology and reverse transcriptase polymerase chain reaction for tyrosinase mRNA
Vitiligo: análise da área enxertada comparada com curetagem isolada através de morfologia celular e transcriptase reversa da reação de polimerase em cadeia do RNA mensageiro da tirosinase
title Vitiligo: analysis of grafting versus curettage alone, using melanocyte morphology and reverse transcriptase polymerase chain reaction for tyrosinase mRNA
spellingShingle Vitiligo: analysis of grafting versus curettage alone, using melanocyte morphology and reverse transcriptase polymerase chain reaction for tyrosinase mRNA
Machado Filho, Carlos D'Apparecida Santos [UNIFESP]
Vitiligo
Transplants
Tyrosinase
Polymerase chain reaction
Pathology
Vitiligo
Transplantes
Tirosinase
Reação em cadeia de polimerase
Patologia
title_short Vitiligo: analysis of grafting versus curettage alone, using melanocyte morphology and reverse transcriptase polymerase chain reaction for tyrosinase mRNA
title_full Vitiligo: analysis of grafting versus curettage alone, using melanocyte morphology and reverse transcriptase polymerase chain reaction for tyrosinase mRNA
title_fullStr Vitiligo: analysis of grafting versus curettage alone, using melanocyte morphology and reverse transcriptase polymerase chain reaction for tyrosinase mRNA
title_full_unstemmed Vitiligo: analysis of grafting versus curettage alone, using melanocyte morphology and reverse transcriptase polymerase chain reaction for tyrosinase mRNA
title_sort Vitiligo: analysis of grafting versus curettage alone, using melanocyte morphology and reverse transcriptase polymerase chain reaction for tyrosinase mRNA
author Machado Filho, Carlos D'Apparecida Santos [UNIFESP]
author_facet Machado Filho, Carlos D'Apparecida Santos [UNIFESP]
Almeida, Fernando Augusto [UNIFESP]
Proto, Rodrigo Sestito
Landman, Gilles [UNIFESP]
author_role author
author2 Almeida, Fernando Augusto [UNIFESP]
Proto, Rodrigo Sestito
Landman, Gilles [UNIFESP]
author2_role author
author
author
dc.contributor.none.fl_str_mv Fundação do ABC Faculdade de Medicina Department of Dermatology
Universidade Federal de São Paulo (UNIFESP)
Hospital do Câncer Treatment and Research Center
dc.contributor.author.fl_str_mv Machado Filho, Carlos D'Apparecida Santos [UNIFESP]
Almeida, Fernando Augusto [UNIFESP]
Proto, Rodrigo Sestito
Landman, Gilles [UNIFESP]
dc.subject.por.fl_str_mv Vitiligo
Transplants
Tyrosinase
Polymerase chain reaction
Pathology
Vitiligo
Transplantes
Tirosinase
Reação em cadeia de polimerase
Patologia
topic Vitiligo
Transplants
Tyrosinase
Polymerase chain reaction
Pathology
Vitiligo
Transplantes
Tirosinase
Reação em cadeia de polimerase
Patologia
description CONTEXT AND OBJECTIVE: Recent studies have indicated that vitiligo areas contain inactive or dormant melanocytes. Melanin synthesis is related to tyrosinase presence and indicative of active metabolic state. The aim of this study was to compare repigmentation, epidermal melanocyte distribution and tyrosinase mRNA detection through reverse transcriptase polymerase chain reaction, in tissue samples of vitiligo, before and after curettage, with or without subsequent autologous skin graft using a new method. DESIGN AND SETTING: Prospective, in the Department of Dermatology, Faculdade de Medicina do ABC, Santo André. METHODS: Two vitiligo areas were curetted. One subsequently received grafted normal sacral autologous skin, whereas the other had no further treatment. The curetted areas were examined after 30 days, to evaluate the degree of repigmentation. The melanocyte percentages and tyrosinase mRNA presence in normal skin and vitiligo areas, before and after curettage and grafting, were compared. RESULTS: Complete repigmentation was seen in all grafted areas, whereas non-grafted curetted vitiligo presented partial repigmentation. The melanocyte percentage in grafted areas was greater than in non-treated vitiligo skin (p = 0.01) and skin with curettage alone (p = 0.015). Tyrosinase mRNA was negative in 93.75% of non-treated vitiligo areas. After treatment (curettage alone or curettage and grafting), all lesions became positive for tyrosinase mRNA. CONCLUSION: Metabolically inactive or dormant melanocytes are probably present within vitiligo areas, and may be activated by exogenous or endogenous stimuli.
publishDate 2005
dc.date.none.fl_str_mv 2005-01-01
2015-06-14T13:31:24Z
2015-06-14T13:31:24Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1516-31802005000400006
São Paulo Medical Journal. Associação Paulista de Medicina - APM, v. 123, n. 4, p. 187-191, 2005.
10.1590/S1516-31802005000400006
S1516-31802005000400006.pdf
1516-3180
S1516-31802005000400006
http://repositorio.unifesp.br/handle/11600/2366
url http://dx.doi.org/10.1590/S1516-31802005000400006
http://repositorio.unifesp.br/handle/11600/2366
identifier_str_mv São Paulo Medical Journal. Associação Paulista de Medicina - APM, v. 123, n. 4, p. 187-191, 2005.
10.1590/S1516-31802005000400006
S1516-31802005000400006.pdf
1516-3180
S1516-31802005000400006
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv São Paulo Medical Journal
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 187-191
application/pdf
dc.publisher.none.fl_str_mv Associação Paulista de Medicina - APM
publisher.none.fl_str_mv Associação Paulista de Medicina - APM
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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