Genotypic and phenotypic analysis of diarrheagenic Escherichia coli strains isolated from Brazilian children living in low socioeconomic level communities
Autor(a) principal: | |
---|---|
Data de Publicação: | 2013 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/36746 http://dx.doi.org/10.1186/1471-2334-13-418 |
Resumo: | Background: Childhood diarrheal diseases remain highly endemic in developing areas of Brazil. the importance of Escherichia coli among children with diarrhea in these areas was unknown. This study determined the prevalence of different E. coli categories in symptomatic and asymptomatic children from low socioeconomic level rural communities in southeastern Brazil.Methods: A total of 560 stool samples were collected from 141 children with diarrhea (< 10 years) and 419 apparently healthy controls who resided in 23 communities. E. coli isolates (n = 1943) were subjected to two multiplex PCRs developed for the detection of enteropathogenic E. coli (EPEC), enteroaggregative E. coli (EAEC), diffusely adherent E. coli (DAEC), enterotoxigenic E. coli (ETEC), enteroinvasive E. coli (EIEC), and Shiga toxin-producing E. coli (STEC). Strains were also examined for the presence of EPEC, EAEC, and DAEC by assays of adhesion to HEp-2 cells and by hybridization with specific DNA probes.Results: Diarrheagenic E. coli strains were isolated from 253 (45.2%) children, and were associated with diarrhea in children aged < 5 years (p < 0.001). EAEC (20.9%), DAEC (11.6%), EPEC (9.3%) were the most frequent pathotypes, followed by ETEC (2.7%), EIEC (0.5%), and STEC (0.2%). Depending of the assay, EPEC, EAEC, and DAEC (collectively termed enteroadherent E. coli) strains were isolated in 45% to 56% of diarrhea cases, a significantly higher incidence than in controls (P < 0.05). Individually, only DAEC showed significant association with diarrhea (p < 0.05), particularly in children aged 2-5 years.Conclusion: This study indicates that enteroadherent E. coli is an important cause of diarrhea in children living in low socioeconomic level communities in southeastern Brazil. Our results reveal that the PCR1 assay is an excellent tool for the identification of EAEC and DAEC. |
id |
UFSP_6362a8fcc0e787c971df8f732b989758 |
---|---|
oai_identifier_str |
oai:repositorio.unifesp.br:11600/36746 |
network_acronym_str |
UFSP |
network_name_str |
Repositório Institucional da UNIFESP |
repository_id_str |
3465 |
spelling |
Lozer, Diego M.Souza, Tamara B. [UNIFESP]Monfardini, Mariane V.Vicentini, FernandoKitagawa, Sonia S.Scaletsky, Isabel Cristina Affonso [UNIFESP]Spano, Liliana C.Univ Fed Espirito SantoUniversidade Federal de São Paulo (UNIFESP)2016-01-24T14:34:24Z2016-01-24T14:34:24Z2013-09-08Bmc Infectious Diseases. London: Biomed Central Ltd, v. 13, 6 p., 2013.1471-2334http://repositorio.unifesp.br/handle/11600/36746http://dx.doi.org/10.1186/1471-2334-13-418WOS000324264400002.pdf10.1186/1471-2334-13-418WOS:000324264400002Background: Childhood diarrheal diseases remain highly endemic in developing areas of Brazil. the importance of Escherichia coli among children with diarrhea in these areas was unknown. This study determined the prevalence of different E. coli categories in symptomatic and asymptomatic children from low socioeconomic level rural communities in southeastern Brazil.Methods: A total of 560 stool samples were collected from 141 children with diarrhea (< 10 years) and 419 apparently healthy controls who resided in 23 communities. E. coli isolates (n = 1943) were subjected to two multiplex PCRs developed for the detection of enteropathogenic E. coli (EPEC), enteroaggregative E. coli (EAEC), diffusely adherent E. coli (DAEC), enterotoxigenic E. coli (ETEC), enteroinvasive E. coli (EIEC), and Shiga toxin-producing E. coli (STEC). Strains were also examined for the presence of EPEC, EAEC, and DAEC by assays of adhesion to HEp-2 cells and by hybridization with specific DNA probes.Results: Diarrheagenic E. coli strains were isolated from 253 (45.2%) children, and were associated with diarrhea in children aged < 5 years (p < 0.001). EAEC (20.9%), DAEC (11.6%), EPEC (9.3%) were the most frequent pathotypes, followed by ETEC (2.7%), EIEC (0.5%), and STEC (0.2%). Depending of the assay, EPEC, EAEC, and DAEC (collectively termed enteroadherent E. coli) strains were isolated in 45% to 56% of diarrhea cases, a significantly higher incidence than in controls (P < 0.05). Individually, only DAEC showed significant association with diarrhea (p < 0.05), particularly in children aged 2-5 years.Conclusion: This study indicates that enteroadherent E. coli is an important cause of diarrhea in children living in low socioeconomic level communities in southeastern Brazil. Our results reveal that the PCR1 assay is an excellent tool for the identification of EAEC and DAEC.Fundacao de Amparo a Pesquisa do Estado de Espirito Santo (FAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Fed Espirito Santo, Dept Patol, Espirito Santo, BrazilUniversidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, BR-04023062 São Paulo, BrazilUniv Fed Espirito Santo, Dept Ciencias Saude, Espirito Santo, BrazilUniversidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, BR-04023062 São Paulo, BrazilWeb of Science6engBiomed Central LtdBmc Infectious DiseasesGenotypic and phenotypic analysis of diarrheagenic Escherichia coli strains isolated from Brazilian children living in low socioeconomic level communitiesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000324264400002.pdfapplication/pdf191687${dspace.ui.url}/bitstream/11600/36746/1/WOS000324264400002.pdf539f902608176af0c34b0e3bf8f16e22MD51open accessTEXTWOS000324264400002.pdf.txtWOS000324264400002.pdf.txtExtracted texttext/plain27724${dspace.ui.url}/bitstream/11600/36746/2/WOS000324264400002.pdf.txtf85ebc0cbc6b4c2c6e4e60d6f45e7cb9MD52open access11600/367462022-09-27 10:14:41.601open accessoai:repositorio.unifesp.br:11600/36746Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-05-25T12:24:49.891628Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Genotypic and phenotypic analysis of diarrheagenic Escherichia coli strains isolated from Brazilian children living in low socioeconomic level communities |
title |
Genotypic and phenotypic analysis of diarrheagenic Escherichia coli strains isolated from Brazilian children living in low socioeconomic level communities |
spellingShingle |
Genotypic and phenotypic analysis of diarrheagenic Escherichia coli strains isolated from Brazilian children living in low socioeconomic level communities Lozer, Diego M. |
title_short |
Genotypic and phenotypic analysis of diarrheagenic Escherichia coli strains isolated from Brazilian children living in low socioeconomic level communities |
title_full |
Genotypic and phenotypic analysis of diarrheagenic Escherichia coli strains isolated from Brazilian children living in low socioeconomic level communities |
title_fullStr |
Genotypic and phenotypic analysis of diarrheagenic Escherichia coli strains isolated from Brazilian children living in low socioeconomic level communities |
title_full_unstemmed |
Genotypic and phenotypic analysis of diarrheagenic Escherichia coli strains isolated from Brazilian children living in low socioeconomic level communities |
title_sort |
Genotypic and phenotypic analysis of diarrheagenic Escherichia coli strains isolated from Brazilian children living in low socioeconomic level communities |
author |
Lozer, Diego M. |
author_facet |
Lozer, Diego M. Souza, Tamara B. [UNIFESP] Monfardini, Mariane V. Vicentini, Fernando Kitagawa, Sonia S. Scaletsky, Isabel Cristina Affonso [UNIFESP] Spano, Liliana C. |
author_role |
author |
author2 |
Souza, Tamara B. [UNIFESP] Monfardini, Mariane V. Vicentini, Fernando Kitagawa, Sonia S. Scaletsky, Isabel Cristina Affonso [UNIFESP] Spano, Liliana C. |
author2_role |
author author author author author author |
dc.contributor.institution.none.fl_str_mv |
Univ Fed Espirito Santo Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Lozer, Diego M. Souza, Tamara B. [UNIFESP] Monfardini, Mariane V. Vicentini, Fernando Kitagawa, Sonia S. Scaletsky, Isabel Cristina Affonso [UNIFESP] Spano, Liliana C. |
description |
Background: Childhood diarrheal diseases remain highly endemic in developing areas of Brazil. the importance of Escherichia coli among children with diarrhea in these areas was unknown. This study determined the prevalence of different E. coli categories in symptomatic and asymptomatic children from low socioeconomic level rural communities in southeastern Brazil.Methods: A total of 560 stool samples were collected from 141 children with diarrhea (< 10 years) and 419 apparently healthy controls who resided in 23 communities. E. coli isolates (n = 1943) were subjected to two multiplex PCRs developed for the detection of enteropathogenic E. coli (EPEC), enteroaggregative E. coli (EAEC), diffusely adherent E. coli (DAEC), enterotoxigenic E. coli (ETEC), enteroinvasive E. coli (EIEC), and Shiga toxin-producing E. coli (STEC). Strains were also examined for the presence of EPEC, EAEC, and DAEC by assays of adhesion to HEp-2 cells and by hybridization with specific DNA probes.Results: Diarrheagenic E. coli strains were isolated from 253 (45.2%) children, and were associated with diarrhea in children aged < 5 years (p < 0.001). EAEC (20.9%), DAEC (11.6%), EPEC (9.3%) were the most frequent pathotypes, followed by ETEC (2.7%), EIEC (0.5%), and STEC (0.2%). Depending of the assay, EPEC, EAEC, and DAEC (collectively termed enteroadherent E. coli) strains were isolated in 45% to 56% of diarrhea cases, a significantly higher incidence than in controls (P < 0.05). Individually, only DAEC showed significant association with diarrhea (p < 0.05), particularly in children aged 2-5 years.Conclusion: This study indicates that enteroadherent E. coli is an important cause of diarrhea in children living in low socioeconomic level communities in southeastern Brazil. Our results reveal that the PCR1 assay is an excellent tool for the identification of EAEC and DAEC. |
publishDate |
2013 |
dc.date.issued.fl_str_mv |
2013-09-08 |
dc.date.accessioned.fl_str_mv |
2016-01-24T14:34:24Z |
dc.date.available.fl_str_mv |
2016-01-24T14:34:24Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Bmc Infectious Diseases. London: Biomed Central Ltd, v. 13, 6 p., 2013. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/36746 http://dx.doi.org/10.1186/1471-2334-13-418 |
dc.identifier.issn.none.fl_str_mv |
1471-2334 |
dc.identifier.file.none.fl_str_mv |
WOS000324264400002.pdf |
dc.identifier.doi.none.fl_str_mv |
10.1186/1471-2334-13-418 |
dc.identifier.wos.none.fl_str_mv |
WOS:000324264400002 |
identifier_str_mv |
Bmc Infectious Diseases. London: Biomed Central Ltd, v. 13, 6 p., 2013. 1471-2334 WOS000324264400002.pdf 10.1186/1471-2334-13-418 WOS:000324264400002 |
url |
http://repositorio.unifesp.br/handle/11600/36746 http://dx.doi.org/10.1186/1471-2334-13-418 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Bmc Infectious Diseases |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
6 |
dc.publisher.none.fl_str_mv |
Biomed Central Ltd |
publisher.none.fl_str_mv |
Biomed Central Ltd |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
bitstream.url.fl_str_mv |
${dspace.ui.url}/bitstream/11600/36746/1/WOS000324264400002.pdf ${dspace.ui.url}/bitstream/11600/36746/2/WOS000324264400002.pdf.txt |
bitstream.checksum.fl_str_mv |
539f902608176af0c34b0e3bf8f16e22 f85ebc0cbc6b4c2c6e4e60d6f45e7cb9 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
|
_version_ |
1783460287800672256 |