Development of a rabbit's urethral sphincter deficiency animal model for anatomical-functional evaluation
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/6966 http://dx.doi.org/10.1590/S1677-55382012000100003 |
Resumo: | OBJECTIVE: The aim of the study was to develop a new durable animal model (using rabbits) for anatomical-functional evaluation of urethral sphincter deficiency. MATERIALS AND METHODS: A total of 40 New Zealand male rabbits, weighting 2.500 kg to 3.100 kg, were evaluated to develop an incontinent animal model. Thirty-two animals underwent urethrolysis and 8 animals received sham operation. Before and at 2, 4, 8 and 12 weeks after urethrolysis or sham operation, it was performed cystometry and leak point pressure (LPP) evaluation with different bladder distension volumes (10, 20, 30 mL). In each time point, 10 animals (8 from the study group and 2 from the sham group) were sacrificed to harvest the bladder and urethra. The samples were evaluated by H&E and Masson's Trichrome to determine urethral morphology and collagen/smooth muscle density. RESULTS: Twelve weeks after urethrolysis, it was observed a significant decrease in LPP regardless the bladder volume (from 33.7 ± 6.6 to 12.8 ± 2.2 cmH2O). The histological analysis evidenced a decrease of 22% in smooth muscle density with a proportional increase in the collagen, vessels and elastin density (p < 0.01). CONCLUSIONS: Transabdominal urethrolysis develops urethral sphincter insufficiency in rabbits, with significant decrease in LPP associated with decrease of smooth muscle fibers and increase of collagen density. This animal model can be used to test autologous cell therapy for stress urinary incontinence treatment. |
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Skaff, M. [UNIFESP]Pinto, E.r.s. [UNIFESP]Leite, Kátia Ramos Moreira [UNIFESP]Almeida, F.g. [UNIFESP]Universidade Federal de São Paulo (UNIFESP)2015-06-14T13:43:36Z2015-06-14T13:43:36Z2012-02-01International braz j urol. Sociedade Brasileira de Urologia, v. 38, n. 1, p. 17-24, 2012.1677-5538http://repositorio.unifesp.br/handle/11600/6966http://dx.doi.org/10.1590/S1677-55382012000100003S1677-55382012000100003.pdfS1677-5538201200010000310.1590/S1677-55382012000100003WOS:000301487700005OBJECTIVE: The aim of the study was to develop a new durable animal model (using rabbits) for anatomical-functional evaluation of urethral sphincter deficiency. MATERIALS AND METHODS: A total of 40 New Zealand male rabbits, weighting 2.500 kg to 3.100 kg, were evaluated to develop an incontinent animal model. Thirty-two animals underwent urethrolysis and 8 animals received sham operation. Before and at 2, 4, 8 and 12 weeks after urethrolysis or sham operation, it was performed cystometry and leak point pressure (LPP) evaluation with different bladder distension volumes (10, 20, 30 mL). In each time point, 10 animals (8 from the study group and 2 from the sham group) were sacrificed to harvest the bladder and urethra. The samples were evaluated by H&E and Masson's Trichrome to determine urethral morphology and collagen/smooth muscle density. RESULTS: Twelve weeks after urethrolysis, it was observed a significant decrease in LPP regardless the bladder volume (from 33.7 ± 6.6 to 12.8 ± 2.2 cmH2O). The histological analysis evidenced a decrease of 22% in smooth muscle density with a proportional increase in the collagen, vessels and elastin density (p < 0.01). CONCLUSIONS: Transabdominal urethrolysis develops urethral sphincter insufficiency in rabbits, with significant decrease in LPP associated with decrease of smooth muscle fibers and increase of collagen density. This animal model can be used to test autologous cell therapy for stress urinary incontinence treatment.Federal University of São Paulo School of Medicine Department of UrologyUNIFESP, EPM, Department of UrologySciELO17-24engSociedade Brasileira de UrologiaInternational braz j urolStem cellstissue engineeringurinary incontinenceanimal modeltransabdominal urethrolysisurethral sphincter deficiencyDevelopment of a rabbit's urethral sphincter deficiency animal model for anatomical-functional evaluationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALS1677-55382012000100003.pdfapplication/pdf403529${dspace.ui.url}/bitstream/11600/6966/1/S1677-55382012000100003.pdf31a2672dddfe4053d7c6e115d455b8d6MD51open accessTEXTS1677-55382012000100003.pdf.txtS1677-55382012000100003.pdf.txtExtracted texttext/plain25769${dspace.ui.url}/bitstream/11600/6966/2/S1677-55382012000100003.pdf.txt0d85fea2ce0cfaf7008bd11b53188f6cMD52open access11600/69662023-01-30 22:19:12.177open accessoai:repositorio.unifesp.br:11600/6966Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-01-31T01:19:12Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Development of a rabbit's urethral sphincter deficiency animal model for anatomical-functional evaluation |
title |
Development of a rabbit's urethral sphincter deficiency animal model for anatomical-functional evaluation |
spellingShingle |
Development of a rabbit's urethral sphincter deficiency animal model for anatomical-functional evaluation Skaff, M. [UNIFESP] Stem cells tissue engineering urinary incontinence animal model transabdominal urethrolysis urethral sphincter deficiency |
title_short |
Development of a rabbit's urethral sphincter deficiency animal model for anatomical-functional evaluation |
title_full |
Development of a rabbit's urethral sphincter deficiency animal model for anatomical-functional evaluation |
title_fullStr |
Development of a rabbit's urethral sphincter deficiency animal model for anatomical-functional evaluation |
title_full_unstemmed |
Development of a rabbit's urethral sphincter deficiency animal model for anatomical-functional evaluation |
title_sort |
Development of a rabbit's urethral sphincter deficiency animal model for anatomical-functional evaluation |
author |
Skaff, M. [UNIFESP] |
author_facet |
Skaff, M. [UNIFESP] Pinto, E.r.s. [UNIFESP] Leite, Kátia Ramos Moreira [UNIFESP] Almeida, F.g. [UNIFESP] |
author_role |
author |
author2 |
Pinto, E.r.s. [UNIFESP] Leite, Kátia Ramos Moreira [UNIFESP] Almeida, F.g. [UNIFESP] |
author2_role |
author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Skaff, M. [UNIFESP] Pinto, E.r.s. [UNIFESP] Leite, Kátia Ramos Moreira [UNIFESP] Almeida, F.g. [UNIFESP] |
dc.subject.eng.fl_str_mv |
Stem cells tissue engineering urinary incontinence animal model transabdominal urethrolysis urethral sphincter deficiency |
topic |
Stem cells tissue engineering urinary incontinence animal model transabdominal urethrolysis urethral sphincter deficiency |
description |
OBJECTIVE: The aim of the study was to develop a new durable animal model (using rabbits) for anatomical-functional evaluation of urethral sphincter deficiency. MATERIALS AND METHODS: A total of 40 New Zealand male rabbits, weighting 2.500 kg to 3.100 kg, were evaluated to develop an incontinent animal model. Thirty-two animals underwent urethrolysis and 8 animals received sham operation. Before and at 2, 4, 8 and 12 weeks after urethrolysis or sham operation, it was performed cystometry and leak point pressure (LPP) evaluation with different bladder distension volumes (10, 20, 30 mL). In each time point, 10 animals (8 from the study group and 2 from the sham group) were sacrificed to harvest the bladder and urethra. The samples were evaluated by H&E and Masson's Trichrome to determine urethral morphology and collagen/smooth muscle density. RESULTS: Twelve weeks after urethrolysis, it was observed a significant decrease in LPP regardless the bladder volume (from 33.7 ± 6.6 to 12.8 ± 2.2 cmH2O). The histological analysis evidenced a decrease of 22% in smooth muscle density with a proportional increase in the collagen, vessels and elastin density (p < 0.01). CONCLUSIONS: Transabdominal urethrolysis develops urethral sphincter insufficiency in rabbits, with significant decrease in LPP associated with decrease of smooth muscle fibers and increase of collagen density. This animal model can be used to test autologous cell therapy for stress urinary incontinence treatment. |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012-02-01 |
dc.date.accessioned.fl_str_mv |
2015-06-14T13:43:36Z |
dc.date.available.fl_str_mv |
2015-06-14T13:43:36Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
International braz j urol. Sociedade Brasileira de Urologia, v. 38, n. 1, p. 17-24, 2012. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/6966 http://dx.doi.org/10.1590/S1677-55382012000100003 |
dc.identifier.issn.none.fl_str_mv |
1677-5538 |
dc.identifier.file.none.fl_str_mv |
S1677-55382012000100003.pdf |
dc.identifier.scielo.none.fl_str_mv |
S1677-55382012000100003 |
dc.identifier.doi.none.fl_str_mv |
10.1590/S1677-55382012000100003 |
dc.identifier.wos.none.fl_str_mv |
WOS:000301487700005 |
identifier_str_mv |
International braz j urol. Sociedade Brasileira de Urologia, v. 38, n. 1, p. 17-24, 2012. 1677-5538 S1677-55382012000100003.pdf S1677-55382012000100003 10.1590/S1677-55382012000100003 WOS:000301487700005 |
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http://repositorio.unifesp.br/handle/11600/6966 http://dx.doi.org/10.1590/S1677-55382012000100003 |
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17-24 |
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Sociedade Brasileira de Urologia |
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Sociedade Brasileira de Urologia |
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