Flagellar Cap Protein FliD Mediates Adherence of Atypical Enteropathogenic Escherichia coli to Enterocyte Microvilli
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2016 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UNIFESP |
| Texto Completo: | http://dx.doi.org/10.1128/IAI.01001-15 https://repositorio.unifesp.br/handle/11600/56093 |
Resumo: | The expression of flagella correlates with different aspects of bacterial pathogenicity, ranging from adherence to host cells to activation of inflammatory responses by the innate immune system. In the present study, we investigated the role of flagella in the adherence of an atypical enteropathogenic Escherichia coli (aEPEC) strain (serotype O51: H40) to human enterocytes. Accordingly, isogenic mutants deficient in flagellin (FliC), the flagellar structural subunit |
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Flagellar Cap Protein FliD Mediates Adherence of Atypical Enteropathogenic Escherichia coli to Enterocyte MicrovilliThe expression of flagella correlates with different aspects of bacterial pathogenicity, ranging from adherence to host cells to activation of inflammatory responses by the innate immune system. In the present study, we investigated the role of flagella in the adherence of an atypical enteropathogenic Escherichia coli (aEPEC) strain (serotype O51: H40) to human enterocytes. Accordingly, isogenic mutants deficient in flagellin (FliC), the flagellar structural subunitthe flagellar cap protein (FliD)or the MotAB proteins, involved in the control of flagellar motion, were generated and tested for binding to differentiated Caco-2 cells. Binding of the aEPEC strain to enterocytes was significantly impaired in strains with the fliC and fliD genes deleted, both of which could not form flagella on the bacterial surface. A nonmotile but flagellated MotAB mutant also showed impaired adhesion to Caco-2 cells. In accordance with these observations, adhesion of aEPEC strain 1711-4 to Caco-2 cells was drastically reduced after the treatment of Caco-2 cells with purified FliD. In addition, incubation of aEPEC bacteria with specific anti-FliD serum impaired binding to Caco-2 cells. Finally, incubation of Caco-2 cells with purified FliD, followed by immunolabeling, showed that the protein was specifically bound to the microvillus tips of differentiated Caco-2 cells. The aEPEC FliD or anti-FliD serum also reduced the adherence of prototype typical enteropathogenic, enterohemorrhagic, and enterotoxigenic E. coli strains to Caco-2 cells. In conclusion, our findings further strengthened the role of flagella in the adherence of aEPEC to human enterocytes and disclosed the relevant structural and functional involvement of FliD in the adhesion process.Univ Fed Sao Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, Sao Paulo, BrazilUniv Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, BR-05508 Sao Paulo, BrazilUniv Fed Sao Paulo, Escola Paulista Med, Ctr Microscopia Eletron, Sao Paulo, BrazilUniv Sao Paulo, Fac Ciencias Farmaceut, Sao Paulo, BrazilInst Fleury Ensino & Pesquisa, Sao Paulo, BrazilFac Ciencias Med Santa Casa Sao Paulo, Dept Patol, Div Microbiol, Sao Paulo, BrazilUniv Estadual Santa Cruz, Ctr Biotecnol & Genet, Ilheus, Bahia, BrazilUniv Fed Sao Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, Sao Paulo, BrazilUniv Fed Sao Paulo, Escola Paulista Med, Ctr Microscopia Eletron, Sao Paulo, BrazilWeb of ScienceSao Paulo Research Foundation (FAPESP)Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)FAPESP: 2011/12664-5FAPESP: 2009/50399-1CNPq: 150833/2012-1Amer Soc Microbiology2020-07-22T13:23:12Z2020-07-22T13:23:12Z2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion1112-1122http://dx.doi.org/10.1128/IAI.01001-15Infection And Immunity. Washington, v. 84, n. 4, p. 1112-1122, 2016.10.1128/IAI.01001-150019-9567https://repositorio.unifesp.br/handle/11600/56093WOS:000377103600023engInfection And ImmunityWashingtoninfo:eu-repo/semantics/openAccessSampaio, Suely Carlos Ferreira [UNIFESP]Luiz, Wilson B.Vieira, Monica Aparecida Midolli [UNIFESP]Ferreira, Rita C. C.Garcia, Bruna Gil [UNIFESP]Sinigaglia-Coimbra, Rita [UNIFESP]Sampaio, Jorge L. M.Ferreira, Luis C. S.Gomes, Tania Aparecida Tardelli [UNIFESP]reponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2022-02-08T11:55:51Zoai:repositorio.unifesp.br/:11600/56093Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652022-02-08T11:55:51Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
| dc.title.none.fl_str_mv |
Flagellar Cap Protein FliD Mediates Adherence of Atypical Enteropathogenic Escherichia coli to Enterocyte Microvilli |
| title |
Flagellar Cap Protein FliD Mediates Adherence of Atypical Enteropathogenic Escherichia coli to Enterocyte Microvilli |
| spellingShingle |
Flagellar Cap Protein FliD Mediates Adherence of Atypical Enteropathogenic Escherichia coli to Enterocyte Microvilli Sampaio, Suely Carlos Ferreira [UNIFESP] |
| title_short |
Flagellar Cap Protein FliD Mediates Adherence of Atypical Enteropathogenic Escherichia coli to Enterocyte Microvilli |
| title_full |
Flagellar Cap Protein FliD Mediates Adherence of Atypical Enteropathogenic Escherichia coli to Enterocyte Microvilli |
| title_fullStr |
Flagellar Cap Protein FliD Mediates Adherence of Atypical Enteropathogenic Escherichia coli to Enterocyte Microvilli |
| title_full_unstemmed |
Flagellar Cap Protein FliD Mediates Adherence of Atypical Enteropathogenic Escherichia coli to Enterocyte Microvilli |
| title_sort |
Flagellar Cap Protein FliD Mediates Adherence of Atypical Enteropathogenic Escherichia coli to Enterocyte Microvilli |
| author |
Sampaio, Suely Carlos Ferreira [UNIFESP] |
| author_facet |
Sampaio, Suely Carlos Ferreira [UNIFESP] Luiz, Wilson B. Vieira, Monica Aparecida Midolli [UNIFESP] Ferreira, Rita C. C. Garcia, Bruna Gil [UNIFESP] Sinigaglia-Coimbra, Rita [UNIFESP] Sampaio, Jorge L. M. Ferreira, Luis C. S. Gomes, Tania Aparecida Tardelli [UNIFESP] |
| author_role |
author |
| author2 |
Luiz, Wilson B. Vieira, Monica Aparecida Midolli [UNIFESP] Ferreira, Rita C. C. Garcia, Bruna Gil [UNIFESP] Sinigaglia-Coimbra, Rita [UNIFESP] Sampaio, Jorge L. M. Ferreira, Luis C. S. Gomes, Tania Aparecida Tardelli [UNIFESP] |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Sampaio, Suely Carlos Ferreira [UNIFESP] Luiz, Wilson B. Vieira, Monica Aparecida Midolli [UNIFESP] Ferreira, Rita C. C. Garcia, Bruna Gil [UNIFESP] Sinigaglia-Coimbra, Rita [UNIFESP] Sampaio, Jorge L. M. Ferreira, Luis C. S. Gomes, Tania Aparecida Tardelli [UNIFESP] |
| description |
The expression of flagella correlates with different aspects of bacterial pathogenicity, ranging from adherence to host cells to activation of inflammatory responses by the innate immune system. In the present study, we investigated the role of flagella in the adherence of an atypical enteropathogenic Escherichia coli (aEPEC) strain (serotype O51: H40) to human enterocytes. Accordingly, isogenic mutants deficient in flagellin (FliC), the flagellar structural subunit |
| publishDate |
2016 |
| dc.date.none.fl_str_mv |
2016 2020-07-22T13:23:12Z 2020-07-22T13:23:12Z |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1128/IAI.01001-15 Infection And Immunity. Washington, v. 84, n. 4, p. 1112-1122, 2016. 10.1128/IAI.01001-15 0019-9567 https://repositorio.unifesp.br/handle/11600/56093 WOS:000377103600023 |
| url |
http://dx.doi.org/10.1128/IAI.01001-15 https://repositorio.unifesp.br/handle/11600/56093 |
| identifier_str_mv |
Infection And Immunity. Washington, v. 84, n. 4, p. 1112-1122, 2016. 10.1128/IAI.01001-15 0019-9567 WOS:000377103600023 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Infection And Immunity |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
1112-1122 |
| dc.coverage.none.fl_str_mv |
Washington |
| dc.publisher.none.fl_str_mv |
Amer Soc Microbiology |
| publisher.none.fl_str_mv |
Amer Soc Microbiology |
| dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
| instname_str |
Universidade Federal de São Paulo (UNIFESP) |
| instacron_str |
UNIFESP |
| institution |
UNIFESP |
| reponame_str |
Repositório Institucional da UNIFESP |
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Repositório Institucional da UNIFESP |
| repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
| repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
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1814268432585064448 |