Insights into the origin of the high variability of multivalent-meiotic associations in holocentric chromosomes of Tityus (Archaeotityus) scorpions
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | https://repositorio.unifesp.br/handle/11600/54115 http://dx.doi.org/10.1371/journal.pone.0192070 |
Resumo: | Scorpions represent an intriguing group of animals characterized by a high incidence of heterozygous chromosomal rearrangements. In this work, we examined six species of Tityus (Archaeotityus) from Brazilian fauna with a particular focus on elucidating the rearrangements responsible for the intraspecific variability of diploid number and the presence of long chromosomal chains in meiosis. To access any interpopulation diversity, we also studied individuals from four species representing distinct localities. Most species demonstrated intraspecific polymorphism in diploid number (2n = 19 and 2n = 20 in T. clathratus, T. mattogrossensis, and T. pusillus, 2n = 16, 2n = 17 and 2n = 18 in T. paraguayensis, and 2n = 16 and 2n = 24 in T. silvestris) and multi-chromosomal associations during meiosis I, which differed even among individuals with the same chromosome number. In some species, the heterozygous rearrangements were not fixed, resulting such as in Tityus clathatrus, in 11 different chromosomal configurations recognized within a same population. Based on meiotic chromosome behaviour, we suggested that independent rearrangements (fusion/fission and reciprocal translocations), occurring in different combinations, originated the multi-chromosomal chains. To evaluate the effects of these chromosome chains on meiotic segregation, we applied the chi-square test in metaphase II cells. The non-significant occurrence of aneuploid nuclei indicated that non-disjunction was negligible in specimens bearing heterozygous rearrangements. Finally, based on our analysis of many chromosome characteristics, e.g., holocentricity, achiasmate meiosis, endopolyploidy, ability to segregate heterosynaptic or unsynapsed chromosomes, (TTAGG) n sequence located in terminal regions of rearranged chromosomes, we suggest that the maintenance of multi-chromosomal associations may be evolutionarily advantageous for these species. |
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Mattos, Viviane FagundesCarvalho Currency, Leonardo SousaCarvalho, Marcos AndreSchneider, Marielle Cristina [UNIFESP]2020-07-08T13:09:39Z2020-07-08T13:09:39Z2018Plos One. San Francisco, v. 13, n. 2, p. -, 2018.1932-6203https://repositorio.unifesp.br/handle/11600/54115http://dx.doi.org/10.1371/journal.pone.0192070WOS000425604300027.pdf10.1371/journal.pone.0192070WOS:000425604300027Scorpions represent an intriguing group of animals characterized by a high incidence of heterozygous chromosomal rearrangements. In this work, we examined six species of Tityus (Archaeotityus) from Brazilian fauna with a particular focus on elucidating the rearrangements responsible for the intraspecific variability of diploid number and the presence of long chromosomal chains in meiosis. To access any interpopulation diversity, we also studied individuals from four species representing distinct localities. Most species demonstrated intraspecific polymorphism in diploid number (2n = 19 and 2n = 20 in T. clathratus, T. mattogrossensis, and T. pusillus, 2n = 16, 2n = 17 and 2n = 18 in T. paraguayensis, and 2n = 16 and 2n = 24 in T. silvestris) and multi-chromosomal associations during meiosis I, which differed even among individuals with the same chromosome number. In some species, the heterozygous rearrangements were not fixed, resulting such as in Tityus clathatrus, in 11 different chromosomal configurations recognized within a same population. Based on meiotic chromosome behaviour, we suggested that independent rearrangements (fusion/fission and reciprocal translocations), occurring in different combinations, originated the multi-chromosomal chains. To evaluate the effects of these chromosome chains on meiotic segregation, we applied the chi-square test in metaphase II cells. The non-significant occurrence of aneuploid nuclei indicated that non-disjunction was negligible in specimens bearing heterozygous rearrangements. Finally, based on our analysis of many chromosome characteristics, e.g., holocentricity, achiasmate meiosis, endopolyploidy, ability to segregate heterosynaptic or unsynapsed chromosomes, (TTAGG) n sequence located in terminal regions of rearranged chromosomes, we suggest that the maintenance of multi-chromosomal associations may be evolutionarily advantageous for these species.Fundacao de Amparo a Pesquisa do Estado de Sao PauloUniv Estadual Paulista, UNESP, Dept Biol, Sao Paulo, BrazilUniv Fed Piaui, UFPI, Campus Almicar Ferreira Sobral Floriano, Piaui, BrazilUniv Fed Mato Grosso, Dept Biol & Zool, Cuiaba, Mato Grosso, BrazilUniv Fed Sao Paulo, UNIFESP, Dept Ecol & Biol Evolut, Sao Paulo, BrazilUniv Fed Minas Gerais, Posgrad Zool, Belo Horizonte, MG, BrazilUniv Fed Sao Paulo, UNIFESP, Dept Ecol & Biol Evolut, Sao Paulo, BrazilFAPESP: 2011/21643-1, 2013/11840-0Web of Science-engPublic Library SciencePlos OneInsights into the origin of the high variability of multivalent-meiotic associations in holocentric chromosomes of Tityus (Archaeotityus) scorpionsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleSan Francisco132info:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000425604300027.pdfapplication/pdf15936724${dspace.ui.url}/bitstream/11600/54115/1/WOS000425604300027.pdfcb19f5ccddcdcc56ed7e51aaa0accaf4MD51open accessTEXTWOS000425604300027.pdf.txtWOS000425604300027.pdf.txtExtracted texttext/plain71350${dspace.ui.url}/bitstream/11600/54115/8/WOS000425604300027.pdf.txt3bce119fe2feffa636841a057e5b8022MD58open accessTHUMBNAILWOS000425604300027.pdf.jpgWOS000425604300027.pdf.jpgIM Thumbnailimage/jpeg7609${dspace.ui.url}/bitstream/11600/54115/10/WOS000425604300027.pdf.jpg174a53de5242ccc3df9db15913c0c513MD510open access11600/541152023-06-05 19:32:05.211open accessoai:repositorio.unifesp.br:11600/54115Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-06-05T22:32:05Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Insights into the origin of the high variability of multivalent-meiotic associations in holocentric chromosomes of Tityus (Archaeotityus) scorpions |
title |
Insights into the origin of the high variability of multivalent-meiotic associations in holocentric chromosomes of Tityus (Archaeotityus) scorpions |
spellingShingle |
Insights into the origin of the high variability of multivalent-meiotic associations in holocentric chromosomes of Tityus (Archaeotityus) scorpions Mattos, Viviane Fagundes |
title_short |
Insights into the origin of the high variability of multivalent-meiotic associations in holocentric chromosomes of Tityus (Archaeotityus) scorpions |
title_full |
Insights into the origin of the high variability of multivalent-meiotic associations in holocentric chromosomes of Tityus (Archaeotityus) scorpions |
title_fullStr |
Insights into the origin of the high variability of multivalent-meiotic associations in holocentric chromosomes of Tityus (Archaeotityus) scorpions |
title_full_unstemmed |
Insights into the origin of the high variability of multivalent-meiotic associations in holocentric chromosomes of Tityus (Archaeotityus) scorpions |
title_sort |
Insights into the origin of the high variability of multivalent-meiotic associations in holocentric chromosomes of Tityus (Archaeotityus) scorpions |
author |
Mattos, Viviane Fagundes |
author_facet |
Mattos, Viviane Fagundes Carvalho Currency, Leonardo Sousa Carvalho, Marcos Andre Schneider, Marielle Cristina [UNIFESP] |
author_role |
author |
author2 |
Carvalho Currency, Leonardo Sousa Carvalho, Marcos Andre Schneider, Marielle Cristina [UNIFESP] |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Mattos, Viviane Fagundes Carvalho Currency, Leonardo Sousa Carvalho, Marcos Andre Schneider, Marielle Cristina [UNIFESP] |
description |
Scorpions represent an intriguing group of animals characterized by a high incidence of heterozygous chromosomal rearrangements. In this work, we examined six species of Tityus (Archaeotityus) from Brazilian fauna with a particular focus on elucidating the rearrangements responsible for the intraspecific variability of diploid number and the presence of long chromosomal chains in meiosis. To access any interpopulation diversity, we also studied individuals from four species representing distinct localities. Most species demonstrated intraspecific polymorphism in diploid number (2n = 19 and 2n = 20 in T. clathratus, T. mattogrossensis, and T. pusillus, 2n = 16, 2n = 17 and 2n = 18 in T. paraguayensis, and 2n = 16 and 2n = 24 in T. silvestris) and multi-chromosomal associations during meiosis I, which differed even among individuals with the same chromosome number. In some species, the heterozygous rearrangements were not fixed, resulting such as in Tityus clathatrus, in 11 different chromosomal configurations recognized within a same population. Based on meiotic chromosome behaviour, we suggested that independent rearrangements (fusion/fission and reciprocal translocations), occurring in different combinations, originated the multi-chromosomal chains. To evaluate the effects of these chromosome chains on meiotic segregation, we applied the chi-square test in metaphase II cells. The non-significant occurrence of aneuploid nuclei indicated that non-disjunction was negligible in specimens bearing heterozygous rearrangements. Finally, based on our analysis of many chromosome characteristics, e.g., holocentricity, achiasmate meiosis, endopolyploidy, ability to segregate heterosynaptic or unsynapsed chromosomes, (TTAGG) n sequence located in terminal regions of rearranged chromosomes, we suggest that the maintenance of multi-chromosomal associations may be evolutionarily advantageous for these species. |
publishDate |
2018 |
dc.date.issued.fl_str_mv |
2018 |
dc.date.accessioned.fl_str_mv |
2020-07-08T13:09:39Z |
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2020-07-08T13:09:39Z |
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article |
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dc.identifier.citation.fl_str_mv |
Plos One. San Francisco, v. 13, n. 2, p. -, 2018. |
dc.identifier.uri.fl_str_mv |
https://repositorio.unifesp.br/handle/11600/54115 http://dx.doi.org/10.1371/journal.pone.0192070 |
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1932-6203 |
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WOS000425604300027.pdf |
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10.1371/journal.pone.0192070 |
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Plos One. San Francisco, v. 13, n. 2, p. -, 2018. 1932-6203 WOS000425604300027.pdf 10.1371/journal.pone.0192070 WOS:000425604300027 |
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https://repositorio.unifesp.br/handle/11600/54115 http://dx.doi.org/10.1371/journal.pone.0192070 |
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