Evaluation of the nitric oxide production in rat renal artery smooth muscle cells culture exposed to radiocontrast agents
Autor(a) principal: | |
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Data de Publicação: | 2004 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/27596 http://dx.doi.org/10.1111/j.1523-1755.2004.00408.x |
Resumo: | Background. Radiocontrast agents (RC), substances largely used in diagnostic procedures, present the nephrotoxicity as one of its major side effects, which could be due to an altered synthesis of vasodilators. the aim of the present study was to evaluate the nitric oxide (NO) production in rat renal artery smooth muscle cells primary culture (rVSMC) exposed to RC.Methods. the cells were treated for 72 hours with mannitol at 10% (MT10; 600 mOsm/kg H2O) or 35% (MT35; 2100 mOsm/kg H2O), with the nonionic iobitridol (IBT), the low-osmolality ioxaglate (IXG), the high-osmolality ioxitalamate (IXT), the nonionic, iso-osmolar iodixanol (IDX), and with lipopolysaccharide (LPS). We determined the NO and osmolality in the cell culture media and the cellular viability.Results. By the Griess and chemiluminescence methods, the NO was not different in MT10 and IDX, but decreased in MT35, IBT, IXG, and IXT when compared with the control; it was increased in LPS and also decreased in all RC + LPS when compared with LPS. MT35, IXT, and IXT + LPS decreased the cellular viability, and the media osmolality was increased in MT35 and IXT compared with the control.Conclusion. the RC (except IDX) significantly reduced NO in rVSMC, which was more pronounced after IXT treatment (57.3%). This was not related to the reduced cell viability (15.8%) or to its high osmolality, because in MT35, with similar osmolality as IXT, NO decreased only 11.0% relatively to the control. Neither the media osmolality nor the cell viability was altered by IXG or IBT. the decreased NO could explain the vasoconstriction and, therefore, the acute renal failure by RC. |
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Ribeiro, LucianeSilva, Fábio Assunção e [UNIFESP]Kurihara, Rose SaemiSchor, Nestor [UNIFESP]Higa, Elisa Mieko Suemitsu [UNIFESP]Universidade Federal de São Paulo (UNIFESP)2016-01-24T12:34:15Z2016-01-24T12:34:15Z2004-02-01Kidney International. Malden: Blackwell Publishing Inc, v. 65, n. 2, p. 589-596, 2004.0085-2538http://repositorio.unifesp.br/handle/11600/27596http://dx.doi.org/10.1111/j.1523-1755.2004.00408.x10.1111/j.1523-1755.2004.00408.xWOS:000187919500024Background. Radiocontrast agents (RC), substances largely used in diagnostic procedures, present the nephrotoxicity as one of its major side effects, which could be due to an altered synthesis of vasodilators. the aim of the present study was to evaluate the nitric oxide (NO) production in rat renal artery smooth muscle cells primary culture (rVSMC) exposed to RC.Methods. the cells were treated for 72 hours with mannitol at 10% (MT10; 600 mOsm/kg H2O) or 35% (MT35; 2100 mOsm/kg H2O), with the nonionic iobitridol (IBT), the low-osmolality ioxaglate (IXG), the high-osmolality ioxitalamate (IXT), the nonionic, iso-osmolar iodixanol (IDX), and with lipopolysaccharide (LPS). We determined the NO and osmolality in the cell culture media and the cellular viability.Results. By the Griess and chemiluminescence methods, the NO was not different in MT10 and IDX, but decreased in MT35, IBT, IXG, and IXT when compared with the control; it was increased in LPS and also decreased in all RC + LPS when compared with LPS. MT35, IXT, and IXT + LPS decreased the cellular viability, and the media osmolality was increased in MT35 and IXT compared with the control.Conclusion. the RC (except IDX) significantly reduced NO in rVSMC, which was more pronounced after IXT treatment (57.3%). This was not related to the reduced cell viability (15.8%) or to its high osmolality, because in MT35, with similar osmolality as IXT, NO decreased only 11.0% relatively to the control. Neither the media osmolality nor the cell viability was altered by IXG or IBT. the decreased NO could explain the vasoconstriction and, therefore, the acute renal failure by RC.Universidade Federal de São Paulo, Escola Paulista Med, Nephrol & Emergency Div, São Paulo, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Nephrol & Emergency Div, São Paulo, BrazilWeb of Science589-596engBlackwell Publishing IncKidney Internationalradiocontrastnitric oxiderenal arteryvascular smooth muscle cells culturenephrotoxic drugsacute renal failureEvaluation of the nitric oxide production in rat renal artery smooth muscle cells culture exposed to radiocontrast agentsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP11600/275962023-01-30 22:17:47.186metadata only accessoai:repositorio.unifesp.br:11600/27596Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-01-31T01:17:47Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Evaluation of the nitric oxide production in rat renal artery smooth muscle cells culture exposed to radiocontrast agents |
title |
Evaluation of the nitric oxide production in rat renal artery smooth muscle cells culture exposed to radiocontrast agents |
spellingShingle |
Evaluation of the nitric oxide production in rat renal artery smooth muscle cells culture exposed to radiocontrast agents Ribeiro, Luciane radiocontrast nitric oxide renal artery vascular smooth muscle cells culture nephrotoxic drugs acute renal failure |
title_short |
Evaluation of the nitric oxide production in rat renal artery smooth muscle cells culture exposed to radiocontrast agents |
title_full |
Evaluation of the nitric oxide production in rat renal artery smooth muscle cells culture exposed to radiocontrast agents |
title_fullStr |
Evaluation of the nitric oxide production in rat renal artery smooth muscle cells culture exposed to radiocontrast agents |
title_full_unstemmed |
Evaluation of the nitric oxide production in rat renal artery smooth muscle cells culture exposed to radiocontrast agents |
title_sort |
Evaluation of the nitric oxide production in rat renal artery smooth muscle cells culture exposed to radiocontrast agents |
author |
Ribeiro, Luciane |
author_facet |
Ribeiro, Luciane Silva, Fábio Assunção e [UNIFESP] Kurihara, Rose Saemi Schor, Nestor [UNIFESP] Higa, Elisa Mieko Suemitsu [UNIFESP] |
author_role |
author |
author2 |
Silva, Fábio Assunção e [UNIFESP] Kurihara, Rose Saemi Schor, Nestor [UNIFESP] Higa, Elisa Mieko Suemitsu [UNIFESP] |
author2_role |
author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Ribeiro, Luciane Silva, Fábio Assunção e [UNIFESP] Kurihara, Rose Saemi Schor, Nestor [UNIFESP] Higa, Elisa Mieko Suemitsu [UNIFESP] |
dc.subject.eng.fl_str_mv |
radiocontrast nitric oxide renal artery vascular smooth muscle cells culture nephrotoxic drugs acute renal failure |
topic |
radiocontrast nitric oxide renal artery vascular smooth muscle cells culture nephrotoxic drugs acute renal failure |
description |
Background. Radiocontrast agents (RC), substances largely used in diagnostic procedures, present the nephrotoxicity as one of its major side effects, which could be due to an altered synthesis of vasodilators. the aim of the present study was to evaluate the nitric oxide (NO) production in rat renal artery smooth muscle cells primary culture (rVSMC) exposed to RC.Methods. the cells were treated for 72 hours with mannitol at 10% (MT10; 600 mOsm/kg H2O) or 35% (MT35; 2100 mOsm/kg H2O), with the nonionic iobitridol (IBT), the low-osmolality ioxaglate (IXG), the high-osmolality ioxitalamate (IXT), the nonionic, iso-osmolar iodixanol (IDX), and with lipopolysaccharide (LPS). We determined the NO and osmolality in the cell culture media and the cellular viability.Results. By the Griess and chemiluminescence methods, the NO was not different in MT10 and IDX, but decreased in MT35, IBT, IXG, and IXT when compared with the control; it was increased in LPS and also decreased in all RC + LPS when compared with LPS. MT35, IXT, and IXT + LPS decreased the cellular viability, and the media osmolality was increased in MT35 and IXT compared with the control.Conclusion. the RC (except IDX) significantly reduced NO in rVSMC, which was more pronounced after IXT treatment (57.3%). This was not related to the reduced cell viability (15.8%) or to its high osmolality, because in MT35, with similar osmolality as IXT, NO decreased only 11.0% relatively to the control. Neither the media osmolality nor the cell viability was altered by IXG or IBT. the decreased NO could explain the vasoconstriction and, therefore, the acute renal failure by RC. |
publishDate |
2004 |
dc.date.issued.fl_str_mv |
2004-02-01 |
dc.date.accessioned.fl_str_mv |
2016-01-24T12:34:15Z |
dc.date.available.fl_str_mv |
2016-01-24T12:34:15Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Kidney International. Malden: Blackwell Publishing Inc, v. 65, n. 2, p. 589-596, 2004. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/27596 http://dx.doi.org/10.1111/j.1523-1755.2004.00408.x |
dc.identifier.issn.none.fl_str_mv |
0085-2538 |
dc.identifier.doi.none.fl_str_mv |
10.1111/j.1523-1755.2004.00408.x |
dc.identifier.wos.none.fl_str_mv |
WOS:000187919500024 |
identifier_str_mv |
Kidney International. Malden: Blackwell Publishing Inc, v. 65, n. 2, p. 589-596, 2004. 0085-2538 10.1111/j.1523-1755.2004.00408.x WOS:000187919500024 |
url |
http://repositorio.unifesp.br/handle/11600/27596 http://dx.doi.org/10.1111/j.1523-1755.2004.00408.x |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Kidney International |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
589-596 |
dc.publisher.none.fl_str_mv |
Blackwell Publishing Inc |
publisher.none.fl_str_mv |
Blackwell Publishing Inc |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
|
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1802764278921428992 |