Lipid bilayer pre-transition as the beginning of the melting process
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://dx.doi.org/10.1016/j.bbamem.2009.01.007 http://repositorio.unifesp.br/handle/11600/31463 |
Resumo: | We investigate the bilayer pre-transition exhibited by some lipids at temperatures below their main phase transition, and which is generally associated to the formation of periodic ripples in the membrane. Experimentally we focus on the anionic lipid dipalmytoylphosphatidylglycerol (DPPG) at different ionic strengths, and on the neutral lipid dipalmytoylphosphatidylcholine (DPPC). From the analysis of differential scanning calorimetry traces of the two lipids we find that both pre- and main transitions are part of the same melting process. Electron spin resonance of spin labels and excitation generalized polarization of Laurdan reveal the coexistence of gel and fluid domains at temperatures between the pre- and main transitions of both lipids, reinforcing the first finding. Also, the melting process of DPPG at low ionic strength is found to be less cooperative than that of DPPC. From the theoretical side, we introduce a statistical model in which a next-nearest-neighbor competing interaction is added to the usual two-state model. for the first time, modulated phases (ordered and disordered lipids periodically aligned) emerge between the gel and fluid phases as a natural consequence of the competition between lipid-lipid interactions. (C) 2009 Elsevier B.V. All rights reserved. |
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Lipid bilayer pre-transition as the beginning of the melting processRipple phaseDSCESRLaurdan fluorescenceStatistical modelMonte Carlo simulationsWe investigate the bilayer pre-transition exhibited by some lipids at temperatures below their main phase transition, and which is generally associated to the formation of periodic ripples in the membrane. Experimentally we focus on the anionic lipid dipalmytoylphosphatidylglycerol (DPPG) at different ionic strengths, and on the neutral lipid dipalmytoylphosphatidylcholine (DPPC). From the analysis of differential scanning calorimetry traces of the two lipids we find that both pre- and main transitions are part of the same melting process. Electron spin resonance of spin labels and excitation generalized polarization of Laurdan reveal the coexistence of gel and fluid domains at temperatures between the pre- and main transitions of both lipids, reinforcing the first finding. Also, the melting process of DPPG at low ionic strength is found to be less cooperative than that of DPPC. From the theoretical side, we introduce a statistical model in which a next-nearest-neighbor competing interaction is added to the usual two-state model. for the first time, modulated phases (ordered and disordered lipids periodically aligned) emerge between the gel and fluid phases as a natural consequence of the competition between lipid-lipid interactions. (C) 2009 Elsevier B.V. All rights reserved.Univ São Paulo, Inst Fis, BR-05315970 São Paulo, BrazilWeb of ScienceFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)DFGElsevier B.V.Universidade de São Paulo (USP)Universidade Federal de São Paulo (UNIFESP)Riske, Karin Amaral [UNIFESP]Barroso, Rafael P.Vequi-Suplicy, Cíntia CristinaGermano, RenatoHenriques, Vera B.Lamy, Maria Teresa2016-01-24T13:52:27Z2016-01-24T13:52:27Z2009-05-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion954-963application/pdfhttp://dx.doi.org/10.1016/j.bbamem.2009.01.007Biochimica Et Biophysica Acta-biomembranes. Amsterdam: Elsevier B.V., v. 1788, n. 5, p. 954-963, 2009.10.1016/j.bbamem.2009.01.007WOS000266190600006.pdf0005-2736http://repositorio.unifesp.br/handle/11600/31463WOS:000266190600006engBiochimica Et Biophysica Acta-biomembranesinfo:eu-repo/semantics/openAccesshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policyreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-07-31T09:04:55Zoai:repositorio.unifesp.br/:11600/31463Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-07-31T09:04:55Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
Lipid bilayer pre-transition as the beginning of the melting process |
title |
Lipid bilayer pre-transition as the beginning of the melting process |
spellingShingle |
Lipid bilayer pre-transition as the beginning of the melting process Riske, Karin Amaral [UNIFESP] Ripple phase DSC ESR Laurdan fluorescence Statistical model Monte Carlo simulations |
title_short |
Lipid bilayer pre-transition as the beginning of the melting process |
title_full |
Lipid bilayer pre-transition as the beginning of the melting process |
title_fullStr |
Lipid bilayer pre-transition as the beginning of the melting process |
title_full_unstemmed |
Lipid bilayer pre-transition as the beginning of the melting process |
title_sort |
Lipid bilayer pre-transition as the beginning of the melting process |
author |
Riske, Karin Amaral [UNIFESP] |
author_facet |
Riske, Karin Amaral [UNIFESP] Barroso, Rafael P. Vequi-Suplicy, Cíntia Cristina Germano, Renato Henriques, Vera B. Lamy, Maria Teresa |
author_role |
author |
author2 |
Barroso, Rafael P. Vequi-Suplicy, Cíntia Cristina Germano, Renato Henriques, Vera B. Lamy, Maria Teresa |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Riske, Karin Amaral [UNIFESP] Barroso, Rafael P. Vequi-Suplicy, Cíntia Cristina Germano, Renato Henriques, Vera B. Lamy, Maria Teresa |
dc.subject.por.fl_str_mv |
Ripple phase DSC ESR Laurdan fluorescence Statistical model Monte Carlo simulations |
topic |
Ripple phase DSC ESR Laurdan fluorescence Statistical model Monte Carlo simulations |
description |
We investigate the bilayer pre-transition exhibited by some lipids at temperatures below their main phase transition, and which is generally associated to the formation of periodic ripples in the membrane. Experimentally we focus on the anionic lipid dipalmytoylphosphatidylglycerol (DPPG) at different ionic strengths, and on the neutral lipid dipalmytoylphosphatidylcholine (DPPC). From the analysis of differential scanning calorimetry traces of the two lipids we find that both pre- and main transitions are part of the same melting process. Electron spin resonance of spin labels and excitation generalized polarization of Laurdan reveal the coexistence of gel and fluid domains at temperatures between the pre- and main transitions of both lipids, reinforcing the first finding. Also, the melting process of DPPG at low ionic strength is found to be less cooperative than that of DPPC. From the theoretical side, we introduce a statistical model in which a next-nearest-neighbor competing interaction is added to the usual two-state model. for the first time, modulated phases (ordered and disordered lipids periodically aligned) emerge between the gel and fluid phases as a natural consequence of the competition between lipid-lipid interactions. (C) 2009 Elsevier B.V. All rights reserved. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-05-01 2016-01-24T13:52:27Z 2016-01-24T13:52:27Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.bbamem.2009.01.007 Biochimica Et Biophysica Acta-biomembranes. Amsterdam: Elsevier B.V., v. 1788, n. 5, p. 954-963, 2009. 10.1016/j.bbamem.2009.01.007 WOS000266190600006.pdf 0005-2736 http://repositorio.unifesp.br/handle/11600/31463 WOS:000266190600006 |
url |
http://dx.doi.org/10.1016/j.bbamem.2009.01.007 http://repositorio.unifesp.br/handle/11600/31463 |
identifier_str_mv |
Biochimica Et Biophysica Acta-biomembranes. Amsterdam: Elsevier B.V., v. 1788, n. 5, p. 954-963, 2009. 10.1016/j.bbamem.2009.01.007 WOS000266190600006.pdf 0005-2736 WOS:000266190600006 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Biochimica Et Biophysica Acta-biomembranes |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy |
dc.format.none.fl_str_mv |
954-963 application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
_version_ |
1814268396984860672 |