Lipid bilayer pre-transition as the beginning of the melting process

Detalhes bibliográficos
Autor(a) principal: Riske, Karin Amaral [UNIFESP]
Data de Publicação: 2009
Outros Autores: Barroso, Rafael P., Vequi-Suplicy, Cíntia Cristina, Germano, Renato, Henriques, Vera B., Lamy, Maria Teresa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1016/j.bbamem.2009.01.007
http://repositorio.unifesp.br/handle/11600/31463
Resumo: We investigate the bilayer pre-transition exhibited by some lipids at temperatures below their main phase transition, and which is generally associated to the formation of periodic ripples in the membrane. Experimentally we focus on the anionic lipid dipalmytoylphosphatidylglycerol (DPPG) at different ionic strengths, and on the neutral lipid dipalmytoylphosphatidylcholine (DPPC). From the analysis of differential scanning calorimetry traces of the two lipids we find that both pre- and main transitions are part of the same melting process. Electron spin resonance of spin labels and excitation generalized polarization of Laurdan reveal the coexistence of gel and fluid domains at temperatures between the pre- and main transitions of both lipids, reinforcing the first finding. Also, the melting process of DPPG at low ionic strength is found to be less cooperative than that of DPPC. From the theoretical side, we introduce a statistical model in which a next-nearest-neighbor competing interaction is added to the usual two-state model. for the first time, modulated phases (ordered and disordered lipids periodically aligned) emerge between the gel and fluid phases as a natural consequence of the competition between lipid-lipid interactions. (C) 2009 Elsevier B.V. All rights reserved.
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spelling Lipid bilayer pre-transition as the beginning of the melting processRipple phaseDSCESRLaurdan fluorescenceStatistical modelMonte Carlo simulationsWe investigate the bilayer pre-transition exhibited by some lipids at temperatures below their main phase transition, and which is generally associated to the formation of periodic ripples in the membrane. Experimentally we focus on the anionic lipid dipalmytoylphosphatidylglycerol (DPPG) at different ionic strengths, and on the neutral lipid dipalmytoylphosphatidylcholine (DPPC). From the analysis of differential scanning calorimetry traces of the two lipids we find that both pre- and main transitions are part of the same melting process. Electron spin resonance of spin labels and excitation generalized polarization of Laurdan reveal the coexistence of gel and fluid domains at temperatures between the pre- and main transitions of both lipids, reinforcing the first finding. Also, the melting process of DPPG at low ionic strength is found to be less cooperative than that of DPPC. From the theoretical side, we introduce a statistical model in which a next-nearest-neighbor competing interaction is added to the usual two-state model. for the first time, modulated phases (ordered and disordered lipids periodically aligned) emerge between the gel and fluid phases as a natural consequence of the competition between lipid-lipid interactions. (C) 2009 Elsevier B.V. All rights reserved.Univ São Paulo, Inst Fis, BR-05315970 São Paulo, BrazilWeb of ScienceFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)DFGElsevier B.V.Universidade de São Paulo (USP)Universidade Federal de São Paulo (UNIFESP)Riske, Karin Amaral [UNIFESP]Barroso, Rafael P.Vequi-Suplicy, Cíntia CristinaGermano, RenatoHenriques, Vera B.Lamy, Maria Teresa2016-01-24T13:52:27Z2016-01-24T13:52:27Z2009-05-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion954-963application/pdfhttp://dx.doi.org/10.1016/j.bbamem.2009.01.007Biochimica Et Biophysica Acta-biomembranes. Amsterdam: Elsevier B.V., v. 1788, n. 5, p. 954-963, 2009.10.1016/j.bbamem.2009.01.007WOS000266190600006.pdf0005-2736http://repositorio.unifesp.br/handle/11600/31463WOS:000266190600006engBiochimica Et Biophysica Acta-biomembranesinfo:eu-repo/semantics/openAccesshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policyreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-07-31T09:04:55Zoai:repositorio.unifesp.br/:11600/31463Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-07-31T09:04:55Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Lipid bilayer pre-transition as the beginning of the melting process
title Lipid bilayer pre-transition as the beginning of the melting process
spellingShingle Lipid bilayer pre-transition as the beginning of the melting process
Riske, Karin Amaral [UNIFESP]
Ripple phase
DSC
ESR
Laurdan fluorescence
Statistical model
Monte Carlo simulations
title_short Lipid bilayer pre-transition as the beginning of the melting process
title_full Lipid bilayer pre-transition as the beginning of the melting process
title_fullStr Lipid bilayer pre-transition as the beginning of the melting process
title_full_unstemmed Lipid bilayer pre-transition as the beginning of the melting process
title_sort Lipid bilayer pre-transition as the beginning of the melting process
author Riske, Karin Amaral [UNIFESP]
author_facet Riske, Karin Amaral [UNIFESP]
Barroso, Rafael P.
Vequi-Suplicy, Cíntia Cristina
Germano, Renato
Henriques, Vera B.
Lamy, Maria Teresa
author_role author
author2 Barroso, Rafael P.
Vequi-Suplicy, Cíntia Cristina
Germano, Renato
Henriques, Vera B.
Lamy, Maria Teresa
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Riske, Karin Amaral [UNIFESP]
Barroso, Rafael P.
Vequi-Suplicy, Cíntia Cristina
Germano, Renato
Henriques, Vera B.
Lamy, Maria Teresa
dc.subject.por.fl_str_mv Ripple phase
DSC
ESR
Laurdan fluorescence
Statistical model
Monte Carlo simulations
topic Ripple phase
DSC
ESR
Laurdan fluorescence
Statistical model
Monte Carlo simulations
description We investigate the bilayer pre-transition exhibited by some lipids at temperatures below their main phase transition, and which is generally associated to the formation of periodic ripples in the membrane. Experimentally we focus on the anionic lipid dipalmytoylphosphatidylglycerol (DPPG) at different ionic strengths, and on the neutral lipid dipalmytoylphosphatidylcholine (DPPC). From the analysis of differential scanning calorimetry traces of the two lipids we find that both pre- and main transitions are part of the same melting process. Electron spin resonance of spin labels and excitation generalized polarization of Laurdan reveal the coexistence of gel and fluid domains at temperatures between the pre- and main transitions of both lipids, reinforcing the first finding. Also, the melting process of DPPG at low ionic strength is found to be less cooperative than that of DPPC. From the theoretical side, we introduce a statistical model in which a next-nearest-neighbor competing interaction is added to the usual two-state model. for the first time, modulated phases (ordered and disordered lipids periodically aligned) emerge between the gel and fluid phases as a natural consequence of the competition between lipid-lipid interactions. (C) 2009 Elsevier B.V. All rights reserved.
publishDate 2009
dc.date.none.fl_str_mv 2009-05-01
2016-01-24T13:52:27Z
2016-01-24T13:52:27Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.bbamem.2009.01.007
Biochimica Et Biophysica Acta-biomembranes. Amsterdam: Elsevier B.V., v. 1788, n. 5, p. 954-963, 2009.
10.1016/j.bbamem.2009.01.007
WOS000266190600006.pdf
0005-2736
http://repositorio.unifesp.br/handle/11600/31463
WOS:000266190600006
url http://dx.doi.org/10.1016/j.bbamem.2009.01.007
http://repositorio.unifesp.br/handle/11600/31463
identifier_str_mv Biochimica Et Biophysica Acta-biomembranes. Amsterdam: Elsevier B.V., v. 1788, n. 5, p. 954-963, 2009.
10.1016/j.bbamem.2009.01.007
WOS000266190600006.pdf
0005-2736
WOS:000266190600006
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Biochimica Et Biophysica Acta-biomembranes
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
eu_rights_str_mv openAccess
rights_invalid_str_mv http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.format.none.fl_str_mv 954-963
application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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