Platelet-derived exosomes from septic shock patients induce myocardial dysfunction

Detalhes bibliográficos
Autor(a) principal: Azevedo, Luciano Cesar Pontes
Data de Publicação: 2007
Outros Autores: Janiszewski, Mariano, Pontieri, Vera, Pedro, Marcelo de Almeida, Bassi, Estevao, Tucci, Paulo Jose Ferreira [UNIFESP], Laurindo, Francisco Rafael Martins
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1186/cc6176
http://repositorio.unifesp.br/handle/11600/29345
Resumo: Introduction Mechanisms underlying inotropic failure in septic shock are incompletely understood. We previously identified the presence of exosomes in the plasma of septic shock patients. These exosomes are released mainly by platelets, produce superoxide, and induce apoptosis in vascular cells by a redox-dependent pathway. We hypothesized that circulating platelet-derived exosomes could contribute to inotropic dysfunction of sepsis.Methods We collected blood samples from 55 patients with septic shock and 12 healthy volunteers for exosome separation. Exosomes from septic patients and healthy individuals were investigated concerning their myocardial depressant effect in isolated heart and papillary muscle preparations.Results Exosomes from the plasma of septic patients significantly decreased positive and negative derivatives of left ventricular pressure in isolated rabbit hearts or developed tension and its first positive derivative in papillary muscles. Exosomes from healthy individuals decreased these variables non-significantly. in hearts from rabbits previously exposed to endotoxin, septic exosomes decreased positive and negative derivatives of ventricular pressure. This negative inotropic effect was fully reversible upon withdrawal of exosomes. Nitric oxide ( NO) production from exosomes derived from septic shock patients was demonstrated by fluorescence. Also, there was an increase in myocardial nitrate content after exposure to septic exosomes.Conclusion Circulating platelet-derived exosomes from septic patients induced myocardial dysfunction in isolated heart and papillary muscle preparations, a phenomenon enhanced by previous in vivo exposure to lipopolysaccharide. the generation of NO by septic exosomes and the increased myocardial nitrate content after incubation with exosomes from septic patients suggest an NO-dependent mechanism that may contribute to myocardial dysfunction of sepsis.
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spelling Platelet-derived exosomes from septic shock patients induce myocardial dysfunctionIntroduction Mechanisms underlying inotropic failure in septic shock are incompletely understood. We previously identified the presence of exosomes in the plasma of septic shock patients. These exosomes are released mainly by platelets, produce superoxide, and induce apoptosis in vascular cells by a redox-dependent pathway. We hypothesized that circulating platelet-derived exosomes could contribute to inotropic dysfunction of sepsis.Methods We collected blood samples from 55 patients with septic shock and 12 healthy volunteers for exosome separation. Exosomes from septic patients and healthy individuals were investigated concerning their myocardial depressant effect in isolated heart and papillary muscle preparations.Results Exosomes from the plasma of septic patients significantly decreased positive and negative derivatives of left ventricular pressure in isolated rabbit hearts or developed tension and its first positive derivative in papillary muscles. Exosomes from healthy individuals decreased these variables non-significantly. in hearts from rabbits previously exposed to endotoxin, septic exosomes decreased positive and negative derivatives of ventricular pressure. This negative inotropic effect was fully reversible upon withdrawal of exosomes. Nitric oxide ( NO) production from exosomes derived from septic shock patients was demonstrated by fluorescence. Also, there was an increase in myocardial nitrate content after exposure to septic exosomes.Conclusion Circulating platelet-derived exosomes from septic patients induced myocardial dysfunction in isolated heart and papillary muscle preparations, a phenomenon enhanced by previous in vivo exposure to lipopolysaccharide. the generation of NO by septic exosomes and the increased myocardial nitrate content after incubation with exosomes from septic patients suggest an NO-dependent mechanism that may contribute to myocardial dysfunction of sepsis.Univ São Paulo, Sch Med, Emergency Med Res Lab, BR-05403900 São Paulo, BrazilHosp Sirio Libanes, Res & Educ Inst, BR-01308060 São Paulo, BrazilUniv São Paulo, Inst Biomed Sci, Dept Pharm, BR-05508900 São Paulo, BrazilUniv São Paulo, Heart Inst InCor, Vasc Biol Lab, BR-05403000 São Paulo, BrazilUniversidade Federal de São Paulo, Cardiovasc Physiol Lab, BR-04022000 São Paulo, BrazilUniversidade Federal de São Paulo, Cardiovasc Physiol Lab, BR-04022000 São Paulo, BrazilWeb of ScienceBiomed Central LtdUniversidade de São Paulo (USP)Hosp Sirio LibanesUniversidade Federal de São Paulo (UNIFESP)Azevedo, Luciano Cesar PontesJaniszewski, MarianoPontieri, VeraPedro, Marcelo de AlmeidaBassi, EstevaoTucci, Paulo Jose Ferreira [UNIFESP]Laurindo, Francisco Rafael Martins2016-01-24T12:41:42Z2016-01-24T12:41:42Z2007-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion10application/pdfhttp://dx.doi.org/10.1186/cc6176Critical Care. London: Biomed Central Ltd, v. 11, n. 6, 10 p., 2007.10.1186/cc6176WOS000253286500004.pdf1466-609Xhttp://repositorio.unifesp.br/handle/11600/29345WOS:000253286500004engCritical Careinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-07T14:12:08Zoai:repositorio.unifesp.br/:11600/29345Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-07T14:12:08Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Platelet-derived exosomes from septic shock patients induce myocardial dysfunction
title Platelet-derived exosomes from septic shock patients induce myocardial dysfunction
spellingShingle Platelet-derived exosomes from septic shock patients induce myocardial dysfunction
Azevedo, Luciano Cesar Pontes
title_short Platelet-derived exosomes from septic shock patients induce myocardial dysfunction
title_full Platelet-derived exosomes from septic shock patients induce myocardial dysfunction
title_fullStr Platelet-derived exosomes from septic shock patients induce myocardial dysfunction
title_full_unstemmed Platelet-derived exosomes from septic shock patients induce myocardial dysfunction
title_sort Platelet-derived exosomes from septic shock patients induce myocardial dysfunction
author Azevedo, Luciano Cesar Pontes
author_facet Azevedo, Luciano Cesar Pontes
Janiszewski, Mariano
Pontieri, Vera
Pedro, Marcelo de Almeida
Bassi, Estevao
Tucci, Paulo Jose Ferreira [UNIFESP]
Laurindo, Francisco Rafael Martins
author_role author
author2 Janiszewski, Mariano
Pontieri, Vera
Pedro, Marcelo de Almeida
Bassi, Estevao
Tucci, Paulo Jose Ferreira [UNIFESP]
Laurindo, Francisco Rafael Martins
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
Hosp Sirio Libanes
Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Azevedo, Luciano Cesar Pontes
Janiszewski, Mariano
Pontieri, Vera
Pedro, Marcelo de Almeida
Bassi, Estevao
Tucci, Paulo Jose Ferreira [UNIFESP]
Laurindo, Francisco Rafael Martins
description Introduction Mechanisms underlying inotropic failure in septic shock are incompletely understood. We previously identified the presence of exosomes in the plasma of septic shock patients. These exosomes are released mainly by platelets, produce superoxide, and induce apoptosis in vascular cells by a redox-dependent pathway. We hypothesized that circulating platelet-derived exosomes could contribute to inotropic dysfunction of sepsis.Methods We collected blood samples from 55 patients with septic shock and 12 healthy volunteers for exosome separation. Exosomes from septic patients and healthy individuals were investigated concerning their myocardial depressant effect in isolated heart and papillary muscle preparations.Results Exosomes from the plasma of septic patients significantly decreased positive and negative derivatives of left ventricular pressure in isolated rabbit hearts or developed tension and its first positive derivative in papillary muscles. Exosomes from healthy individuals decreased these variables non-significantly. in hearts from rabbits previously exposed to endotoxin, septic exosomes decreased positive and negative derivatives of ventricular pressure. This negative inotropic effect was fully reversible upon withdrawal of exosomes. Nitric oxide ( NO) production from exosomes derived from septic shock patients was demonstrated by fluorescence. Also, there was an increase in myocardial nitrate content after exposure to septic exosomes.Conclusion Circulating platelet-derived exosomes from septic patients induced myocardial dysfunction in isolated heart and papillary muscle preparations, a phenomenon enhanced by previous in vivo exposure to lipopolysaccharide. the generation of NO by septic exosomes and the increased myocardial nitrate content after incubation with exosomes from septic patients suggest an NO-dependent mechanism that may contribute to myocardial dysfunction of sepsis.
publishDate 2007
dc.date.none.fl_str_mv 2007-01-01
2016-01-24T12:41:42Z
2016-01-24T12:41:42Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1186/cc6176
Critical Care. London: Biomed Central Ltd, v. 11, n. 6, 10 p., 2007.
10.1186/cc6176
WOS000253286500004.pdf
1466-609X
http://repositorio.unifesp.br/handle/11600/29345
WOS:000253286500004
url http://dx.doi.org/10.1186/cc6176
http://repositorio.unifesp.br/handle/11600/29345
identifier_str_mv Critical Care. London: Biomed Central Ltd, v. 11, n. 6, 10 p., 2007.
10.1186/cc6176
WOS000253286500004.pdf
1466-609X
WOS:000253286500004
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Critical Care
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 10
application/pdf
dc.publisher.none.fl_str_mv Biomed Central Ltd
publisher.none.fl_str_mv Biomed Central Ltd
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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