Histological evidence of reparative activity in chorioamniotic membrane following open fetal surgery for myelomeningocele

• Autor(a) principal: Carvalho, Natalia S. [UNIFESP]
• Data de Publicação: 2017
• Outros Autores: Moron, Antonio F. [UNIFESP], Menon, Ramkumar, Cavalheiro, Sergio [UNIFESP], Barbosa, Mauricio M. [UNIFESP], Milani, Herbene J. [UNIFESP], Ishigai, Marcia M. [UNIFESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNIFESP
• Texto Completo: http://dx.doi.org/10.3892/etm.2017.4976; https://repositorio.unifesp.br/handle/11600/57310
• topic: fetal membrane repair; fetal membrane healing; myelomeningocele; open fetal surgery; spina bifida

An increased understanding of the reparative process in fetal membrane following surgical techniques may be helpful to decrease the risks to mother and fetus and avoid adverse pregnancy outcomes. The present study discusses histological evaluation of the fetal membrane following open fetal surgery. Chorioamniotic membranes (n=10) were obtained following birth from pregnancies that underwent open fetal surgery for myelomeningocele. The collagen distribution was quantified using picrosirius-polarization method comparing the suture site with non-suture site. The differences between the collagen fiber percentages at the two sites was evaluated by the paired t-test with P<0.05. The mean gestational age of fetal surgery was 26.09 +/- 0.3 and 33.81 +/- 0.82 weeks at birth. The picrosirius red sign was more intense at the suture site, primarily associated with collagen type 1. Collagen observed in the surgical area was significantly increased (13.22 +/- 2.84%) compared with the non-surgical area (6.16 +/- 1.09%