Murine Retroviruses Re-engineered for Lineage Tracing and Expression of Toxic Genes in the Developing Chick Embryo
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/30974 http://dx.doi.org/10.1002/dvdy.21766 |
Resumo: | We describe two replication incompetent retroviral vectors that co-express green fluorescent protein (GFP) and beta-galactosidase. These vectors incorporate either the avian reticuloendotheliosis (spleen necrosis virus; SNV) promoter or the chick beta-actin promoter, into the backbone of the murine leukemia (MLV) viral vector. the additional promoters drive transgene expression in avian tissue. the remainder of the vector is MLV-like, allowing high titer viral particle production by means of transient transfection. the SNV promoter produces high and early expression of introduced genes, enabling detection of the single copy integrated GFP gene in infected cells and their progeny in vivo. Substitution of the LacZ coding DNA with a relevant gene of interest will enable its co-expression with GFP, thus allowing visualization of the effect of specific and stable changes in gene expression throughout development. As the VSV-G pseudotyped viral vector is replication incompetent, changes in gene expression can be controlled temporally, by altering the timing of introduction. Developmental Dynamics 237.3260-3269, 2008. Published 2008 Wiley-Liss, Inc. |
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Venters, Sara J.Dias-da-Silva, Magnus Régios [UNIFESP]Hyer, JeanetteUniv Calif San FranciscoUniversidade Federal de São Paulo (UNIFESP)2016-01-24T13:51:47Z2016-01-24T13:51:47Z2008-11-01Developmental Dynamics. Hoboken: Wiley-liss, v. 237, n. 11, p. 3260-3269, 2008.1058-8388http://repositorio.unifesp.br/handle/11600/30974http://dx.doi.org/10.1002/dvdy.2176610.1002/dvdy.21766WOS:000260930400016We describe two replication incompetent retroviral vectors that co-express green fluorescent protein (GFP) and beta-galactosidase. These vectors incorporate either the avian reticuloendotheliosis (spleen necrosis virus; SNV) promoter or the chick beta-actin promoter, into the backbone of the murine leukemia (MLV) viral vector. the additional promoters drive transgene expression in avian tissue. the remainder of the vector is MLV-like, allowing high titer viral particle production by means of transient transfection. the SNV promoter produces high and early expression of introduced genes, enabling detection of the single copy integrated GFP gene in infected cells and their progeny in vivo. Substitution of the LacZ coding DNA with a relevant gene of interest will enable its co-expression with GFP, thus allowing visualization of the effect of specific and stable changes in gene expression throughout development. As the VSV-G pseudotyped viral vector is replication incompetent, changes in gene expression can be controlled temporally, by altering the timing of introduction. Developmental Dynamics 237.3260-3269, 2008. Published 2008 Wiley-Liss, Inc.Univ Calif San Francisco, Dept Neurosurg, San Francisco, CA 94143 USAUniversidade Federal de São Paulo, Escola Paulista Med, Dept Bioquim, São Paulo, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Dept Bioquim, São Paulo, BrazilWeb of Science3260-3269engWiley-BlackwellDevelopmental Dynamicshttp://olabout.wiley.com/WileyCDA/Section/id-406071.htmlinfo:eu-repo/semantics/openAccessretrovirusSNVchicken embryogene expressionlineage tracingembryonic developmentMurine Retroviruses Re-engineered for Lineage Tracing and Expression of Toxic Genes in the Developing Chick Embryoinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlereponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP11600/309742022-06-02 09:02:19.345metadata only accessoai:repositorio.unifesp.br:11600/30974Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652022-06-02T12:02:19Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Murine Retroviruses Re-engineered for Lineage Tracing and Expression of Toxic Genes in the Developing Chick Embryo |
title |
Murine Retroviruses Re-engineered for Lineage Tracing and Expression of Toxic Genes in the Developing Chick Embryo |
spellingShingle |
Murine Retroviruses Re-engineered for Lineage Tracing and Expression of Toxic Genes in the Developing Chick Embryo Venters, Sara J. retrovirus SNV chicken embryo gene expression lineage tracing embryonic development |
title_short |
Murine Retroviruses Re-engineered for Lineage Tracing and Expression of Toxic Genes in the Developing Chick Embryo |
title_full |
Murine Retroviruses Re-engineered for Lineage Tracing and Expression of Toxic Genes in the Developing Chick Embryo |
title_fullStr |
Murine Retroviruses Re-engineered for Lineage Tracing and Expression of Toxic Genes in the Developing Chick Embryo |
title_full_unstemmed |
Murine Retroviruses Re-engineered for Lineage Tracing and Expression of Toxic Genes in the Developing Chick Embryo |
title_sort |
Murine Retroviruses Re-engineered for Lineage Tracing and Expression of Toxic Genes in the Developing Chick Embryo |
author |
Venters, Sara J. |
author_facet |
Venters, Sara J. Dias-da-Silva, Magnus Régios [UNIFESP] Hyer, Jeanette |
author_role |
author |
author2 |
Dias-da-Silva, Magnus Régios [UNIFESP] Hyer, Jeanette |
author2_role |
author author |
dc.contributor.institution.none.fl_str_mv |
Univ Calif San Francisco Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Venters, Sara J. Dias-da-Silva, Magnus Régios [UNIFESP] Hyer, Jeanette |
dc.subject.eng.fl_str_mv |
retrovirus SNV chicken embryo gene expression lineage tracing embryonic development |
topic |
retrovirus SNV chicken embryo gene expression lineage tracing embryonic development |
description |
We describe two replication incompetent retroviral vectors that co-express green fluorescent protein (GFP) and beta-galactosidase. These vectors incorporate either the avian reticuloendotheliosis (spleen necrosis virus; SNV) promoter or the chick beta-actin promoter, into the backbone of the murine leukemia (MLV) viral vector. the additional promoters drive transgene expression in avian tissue. the remainder of the vector is MLV-like, allowing high titer viral particle production by means of transient transfection. the SNV promoter produces high and early expression of introduced genes, enabling detection of the single copy integrated GFP gene in infected cells and their progeny in vivo. Substitution of the LacZ coding DNA with a relevant gene of interest will enable its co-expression with GFP, thus allowing visualization of the effect of specific and stable changes in gene expression throughout development. As the VSV-G pseudotyped viral vector is replication incompetent, changes in gene expression can be controlled temporally, by altering the timing of introduction. Developmental Dynamics 237.3260-3269, 2008. Published 2008 Wiley-Liss, Inc. |
publishDate |
2008 |
dc.date.issued.fl_str_mv |
2008-11-01 |
dc.date.accessioned.fl_str_mv |
2016-01-24T13:51:47Z |
dc.date.available.fl_str_mv |
2016-01-24T13:51:47Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Developmental Dynamics. Hoboken: Wiley-liss, v. 237, n. 11, p. 3260-3269, 2008. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/30974 http://dx.doi.org/10.1002/dvdy.21766 |
dc.identifier.issn.none.fl_str_mv |
1058-8388 |
dc.identifier.doi.none.fl_str_mv |
10.1002/dvdy.21766 |
dc.identifier.wos.none.fl_str_mv |
WOS:000260930400016 |
identifier_str_mv |
Developmental Dynamics. Hoboken: Wiley-liss, v. 237, n. 11, p. 3260-3269, 2008. 1058-8388 10.1002/dvdy.21766 WOS:000260930400016 |
url |
http://repositorio.unifesp.br/handle/11600/30974 http://dx.doi.org/10.1002/dvdy.21766 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Developmental Dynamics |
dc.rights.driver.fl_str_mv |
http://olabout.wiley.com/WileyCDA/Section/id-406071.html info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://olabout.wiley.com/WileyCDA/Section/id-406071.html |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
3260-3269 |
dc.publisher.none.fl_str_mv |
Wiley-Blackwell |
publisher.none.fl_str_mv |
Wiley-Blackwell |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
|
_version_ |
1802764129364082688 |