The role of kinin B-1 and B-2 receptors in the scratching behaviour induced by proteinase-activated receptor-2 agonists in mice

• Autor(a) principal: Costa, Robson
• Data de Publicação: 2010
• Outros Autores: Manjavachi, Marianne N., Motta, Emerson M., Marotta, Denise M., Juliano, Luiz [UNIFESP], Torres, Hugo Arruda de Moura [UNIFESP], Pesquero, João Bosco [UNIFESP], Calixto, Joao B.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNIFESP
• Texto Completo: http://dx.doi.org/10.1111/j.1476-5381.2009.00571.x; http://repositorio.unifesp.br/handle/11600/32190
• topic: PAR-2; scratching behaviour; kinins; kinin B-2 and B-1 receptors

Background and purpose:Activation of the proteinase-activated receptor-2 (PAR-2) induces scratching behaviour in mice. Here, we have investigated the role of kinin B-1 and B-2 receptors in the pruritogenic response elicited by activators of PAR-2.Experimental approach:Scratching was induced by an intradermal (i.d.) injection of trypsin or the selective PAR-2 activating peptide SLIGRL-NH2 at the back of the mouse neck. the animals were observed for 40 min and their scratching response was quantified.Key results:I.d. injection of trypsin or SLIGRL-NH2 evoked a scratching behaviour, dependent on PAR-2 activation. Mice genetically deficient in kinin B-1 or B-2 receptors exhibited reduced scratching behaviour after i.d. injection of trypsin or SLIGRL-NH2. Treatment (i.p.) with the non-peptide B-1 or B-2 receptor antagonists SSR240612 and FR173657, respectively, prevented the scratching behaviour caused by trypsin or SLIGRL-NH2. Nonetheless, only treatment i.p. with the peptide B-2 receptor antagonist, Hoe 140, but not the B-1 receptor antagonist (DALBK), inhibited the pruritogenic response to trypsin. Hoe 140 was also effective against SLIGRL-NH2-induced scratching behaviour when injected by i.d. or intrathecal (i.t.) routes. Also, the response to SLIGRL-NH2 was inhibited by i.t. (but not by i.d.) treatment with DALBK. Conversely, neither Hoe 140 nor DALBK were able to inhibit SLIGRL-NH2-induced scratching behaviour when given intracerebroventricularly (i.c.v.).Conclusions and implications:The present results demonstrated that kinins acting on both B-1 and B-2 receptors played a crucial role in controlling the pruriceptive signalling triggered by PAR-2 activation in mice.